全文获取类型
收费全文 | 333篇 |
免费 | 25篇 |
专业分类
林业 | 44篇 |
农学 | 6篇 |
53篇 | |
综合类 | 6篇 |
农作物 | 10篇 |
水产渔业 | 40篇 |
畜牧兽医 | 182篇 |
园艺 | 5篇 |
植物保护 | 12篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 5篇 |
2019年 | 8篇 |
2018年 | 7篇 |
2017年 | 17篇 |
2016年 | 13篇 |
2015年 | 8篇 |
2014年 | 11篇 |
2013年 | 27篇 |
2012年 | 29篇 |
2011年 | 27篇 |
2010年 | 9篇 |
2009年 | 9篇 |
2008年 | 18篇 |
2007年 | 21篇 |
2006年 | 16篇 |
2005年 | 15篇 |
2004年 | 13篇 |
2003年 | 21篇 |
2002年 | 14篇 |
2001年 | 10篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 7篇 |
1997年 | 1篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 6篇 |
1990年 | 4篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1983年 | 2篇 |
1980年 | 1篇 |
1975年 | 2篇 |
排序方式: 共有358条查询结果,搜索用时 31 毫秒
301.
302.
303.
304.
Atsushi Okazawa Hiroaki Samejima Shigeru Kitani Yukihiro Sugimoto Daisaku Ohta 《Journal of Pesticide Science》2021,46(2):242
Damage caused by Orobanchaceae root parasitic weeds is a substantial agricultural problem for global food security. Many studies have been conducted to establish practical methods of control, but efforts are still required for successful management. Seed germination of root parasitic weeds requires host-derived germination stimulants including strigolactones (SLs). Studies on SLs have revealed that a butenolide ring is the essential moiety for SL activity as a germination stimulant. Interestingly, recent studies have revealed that butenolide hormones regulate the biosynthesis of secondary metabolites and mediate communication in actinomycete bacteria. Because of the structural similarity between SLs and the bacterial butenolides, we evaluated the germination stimulatory activity of butenolides isolated from Streptomyces albus J1074 on root parasitic weeds. These butenolides were found to specifically induce seed germination of Orobanche minor. Our findings contribute to understanding the molecular mechanisms of germination stimulant perception and to the development of a method for their biological control. 相似文献
305.
Constancio A. Asis Jr. Masatsugu Kubota Hiroyuki Ohta Yasuhiro Arima Vladimir K. Chebotar Ken-ichi Tsuchiya 《Soil Science and Plant Nutrition》2013,59(3):759-765
Endophytic bacteria were isolated from the juice of a 6 month-old sugarcane cv. NiF-8, which was collected from Miyako, Okinawa and planted in a field lysimeter in Tsukuba, Ibaraki, Japan. The most probable number of N2-fixing endophytes was 4.5 × 105 cells per gram of fresh weight sample and 21 isolates have a positive reaction for ARA in an N2-free semi-solid medium with 10% crystallized sugarcane sugar and 0.5% sugarcane juice. Analyses of some of the biochemical properties of the N2-fixing isolates indicated that 13 isolates were putative strains of Acetobacter diazotrophicus, 4 isolates showed similar characteristics to those of Herbaspirillum seropedicae, and 4 isolates consisted of Herbaspirillum rubribalbicans-like strains. This study confirmed the existence of N2-fixing endophytic bacteria in the Japanese sugarcane cv. NiF-8. 相似文献
306.
Yasushi KAWAI Kensuke ARAKAWA Ayako ITOH Boku SAITOH Yasuyuki ISHII Junko NISHIMURA Haruki KITAZAWA Takatoshi ITOH Tadao SAITO 《Animal Science Journal》2003,74(1):45-51
Gassericin A, a bacteriocin produced by Lactobacillus gasseri LA39, has a cyclic structure linking N‐ and C‐terminal amino acids. Gassericin A was expressed in Escherichia coli JM109 as a biotinylated fusion protein on the basis of the DNA sequence of mature bacteriocin. A positive clone accumulated the bacteriocin, with no activity, as a soluble fusion protein in the cytoplasm. After release of an N‐terminal tag with factor Xa protease, gassericin A was converted into an active peptide having N‐ and C‐termini. The total amount of purified bacteriocins (expressed and native) was 480 µg/L and 370 µg/L, respectively. However, the specific activity of expressed gassericin A was 15 AU/mg lower than that of native bacteriocin (2600 AU/mg). Although the actual Mr (molecular weight) of the expressed bacteriocin should be 5666, the peptide showed the same mobility (Mr 3800) in sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) as native cyclic gassericin A, suggesting that the expressed peptide retains compact folding of the molecule similar to that of native gassericin A. 相似文献
307.
Y Yamamura M Tamano T Iguchi Y Ohta 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(6):687-689
In various human tumors, a metal binding protein, metallothionein (MT) is reported to play an important role in carcinogenesis. In the present preliminary study, MT expression and tumor growth were investigated in transplantable pregnancy-independent mammary tumors (TPIMT) derived from pregnancy-independent mammary tumors (PIMT) in GR/A mice, in order to study the possible role of MT in mammary carcinogenesis. TPIMT as well as PIMT showed MT expression in tumor cells in all of the successive transplantations. A negative correlation was observed between MT expression in transplanted tumor tissues and their growth in the hosts (r=-0.53, p<0.05). The present study indicates that MT is a useful marker of tumor progression in TPIMT. 相似文献
308.
Haruki Watanabe Makoto Kondo Tomomi Ban‐Tokuda Hiroki Matsui 《Animal Science Journal》2016,87(9):1188-1192
The purpose of this study was to investigate the diversity and fluctuation in the ciliate protozoan population in the rumen of cattle. DNA was extracted from the rumen of three ruminally cannulated, crossbred cattle and a polymerase chain reaction (PCR)‐derived clone library was constructed, using a specific primer set targeting 18S ribosomal RNA genes of ciliate protozoa. DNA fragments of seven selected clones were validated for standard DNA of the protozoa‐specific real‐time PCR assay. Furthermore, population fluctuation of ciliate protozoa and methanogens in the cattle rumen was determined by real‐time PCR. A total of 60 clones were sequenced, phylogenetically analyzed, and classified into 24 operational taxonomic units (OTUs) based on a 99% similarity criterion. More than 80% sequences were phylogenetically placed in the genus Entodinium. The rest of the sequences were placed in the genus Diploplastron (5%), Dasytricha (8.3%) and Isotricha (3.3%). The results suggest that Entodinium was the dominant group in the rumen of cattle used in this study. The ciliate protozoan population showed no significant change in numbers during the monitoring period and reached a peak at 3 h after feeding. Changes in the protozoa population were lower than those of the methanogens. 相似文献
309.
To clarify the inducing mechanism of kerf deviation caused by unsymmetrical set of the handsaw, we hypothesized that the moment
on the bottom edge of the saw blade, which is generated by the unsymmetrical set on handsaw teeth, bends the saw blade and
this deformation should be one factor of the kerf deviation. To verify this hypothesis, the bend of a saw blade was examined
by experiment and by calculation. In the experiments, a model tooth was attached to an actual handsaw. In the calculations,
a saw model with a rectangular prism as the model tooth was analyzed by the finite element method. Loads were applied on the
model tooth or the rectangular prism. From both the experiments and the calculations, the following results were obtained:
(1) the deformation of the handsaw was caused by the unsymmetrical set in the length direction and the height direction of
the saw blade; (2) the deformation and the inclination of the blade was larger at the front part of the saw than at the butt
part; and (3) comparing the saw handle fixing conditions, the kerf deviation in the sawing line was supposed to be larger
in the free handle condition and that kerf deviation in the depth direction was larger in the fixed handle condition. Based
on the above results, it is apparent that one of the kerf deviation mechanisms originated from unsymmetrical set on the saw
teeth.
Part of this report was presented at the 54th Annual Meeting of the Japan Wood Research Society, Sapporo, Japan, August 2004. 相似文献
310.
Quantitative Contrast‐enhanced Ultrasonographic Assessment of Naturally Occurring Pancreatitis in Dogs
下载免费PDF全文
![点击此处可从《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》网站下载免费的PDF全文](/ch/ext_images/free.gif)
S.Y. Lim K. Nakamura K. Morishita N. Sasaki M. Murakami T. Osuga N. Yokoyama H. Ohta M. Yamasaki M. Takiguchi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(1):71-78