Analysis of polar lipids in the serum from rats fed shiitake by liquid chromatography-mass spectrometry/mass spectrometry

Consumption of a shiitake mushroom diet has been reported to have effects on serum phospholipids. However, much less is known about the effect on serum polar lipids including lysophospholipids and free fatty acids. In the present study, the effects of a shiitake diet were evaluated on the basis of identification and quantification of individual polar lipid components in rat serum using liquid chromatography-mass spectrometry/mass spectrometry. By comparison with standards and published data, 50 lysophospholipids and 32 free fatty acids were identified, and the concentrations of 27 polar lipids in rat serum were determined. Shiitake diets decreased the levels of all individual polar lipid components in the serum of male rat. The total level of serum polar lipids in males fed 4% shiitake diets (1365.71 mol/L) was significantly lower than that of the control (2270.26 mol/L). However, shiitake diets did not significantly affect the levels of serum polar lipids in female rats.     

内陆河湿地芦苇叶功能性状特征及其对土壤环境因子的响应

植物功能性状是连接植物与外界环境的重要桥梁，其在不同环境梯度下的变化表现出了对异质环境的适应机制。本文以敦煌西湖国家级自然保护区克隆植物芦苇（Phragmites australis）为研究对象，设置了未退化、轻度退化、中度退化和重度退化4个梯度样地，研究环境异质条件下芦苇叶功能性状特征及其对土壤环境因子的响应。结果表明：① 各环境梯度下，芦苇叶功能性状特征变化显著（P<0.05），叶长、叶宽、叶面积、叶干重随环境梯度退化呈下降趋势，比叶面积随梯度退化呈上升趋势；② 芦苇各叶功能性状间呈协同变化的特征，叶长、叶宽、叶面积、叶干重之间呈极显著正相关（P<0.01），比叶面积与叶长、叶干重呈显著负相关（P<0.05）；③ 叶长、叶宽、叶面积、叶干重与土壤表层（0~30 cm）、中层（30~60 cm）水分呈显著正相关（P<0.05），而与土壤表层、中层含盐量呈显著负相关（P<0.05），说明土壤表层与中层的水分与盐分是芦苇叶功能性状最主要的驱动力。研究结果对干旱区内陆河湿地的保护、生态恢复及管理具有重要的指导意义。     

肉鸭经松香脱毛后表皮松香残留的薄层色谱检测研究

为建立鸭表皮残留松香的检测方法,以模拟鸭厂生产条件对肉鸭进行松香脱毛处理、取样,采用固相萃取(SPE)法对样品进行纯化与富集,以枞酸为松香的标记物质,通过薄层色谱法(TLC)对样品进行鉴定。结果表明:此方法能在鸭表皮检出残留的松香。     

醉酒与解酒的研究

概述酒精在体内的吸收与代谢、醉酒的定义、醉酒机理及解酒原理,介绍主要解酒产品的分类,分析解酒产品市场存在的问题及市场前景,旨在为解酒产品开发提供理论依据。     

聚丙烯酰胺凝胶电泳的一种辅助辨型方法

聚丙烯酰胺凝胶电泳（PAGE）是实验室常用的基本鉴定方法之一。但由于做胶方式以及各实验室仪器设备良莠不齐，经过一系列纷繁复杂的操作，电泳结果未必尽如人意。其中凝胶带型的判断就是一个较为困惑的问题，一般的聚丙烯酰胺胶跑出的条带总体上都有弧度，越是靠近凝胶两侧越是严重。在本实验中，我们设计了一种较为简单易行的方案，即将难以区分的条带样品做成混合样marker来辅助辨型，通过调整电压和凝胶浓度来达到区分条带的目的。我们用该方案可以将相差0～3bp大小的条带区分开来。     

Effects of silicon application on drought resistance of cucumber plants

A study on the effects of silicon supply on the resistance to drought in cucumber plants was conducted in pot experiments. The results suggested that in the absence of stress, silicon slightly enhanced the net photosynthetic rate, but significantly decreased the transpiration rate and stomatal conductance in cucumber plants. Silicon enhanced the net photosynthetic rate of cucumber plants under drought stress. Since silicon decreased the stomatal conductance, enhanced the capacity of holding water, and kept the transpiration rate at a relatively steady rate during drought stress, the photosynthesis of the cucumber plants was sustained. And under drought stress, silicon increased the biomass and water content of leaves in cucumber plants. Silicon decreased the decomposition of chlorophyll in cucumber plants under drought stress, limited the increase of the plasma membrane permeability and malondialdehyde (MDA) content in leaves, alleviated the physiological response of peroxidase (POD) to drought stress, maintained the superoxide dismutase (SOD) normal adaptation, and increased the activity of catalase (CAT). Under severe stress, these physiological biochemical reactions showed positive correlations with the amount of silicon supply. These findings demonstrated that silicon enhanced the resistance of the cucumber plants to drought. Statistical analysis indicated that under drought stress the cumulative value of biomass showed a highly significant correlation with the cumulative value of diurnal photosynthesis (r = 0.9812, p < 0.01), and was significantly correlated with the water content of leaves (r = 0.8650, p < 0.05). These results demonstrated that under drought stress the first factor responsible for the effects of silicon application on the cumulative value of biomass was the increase of photosynthesis, and the second factor was the enhancement of the water holding capacity. Based on these facts, it was concluded that silicon enhanced the resistance to drought mainly by taking part in the metabolism of plants.     

圈养蓝鹇和白鹇繁殖行为的时间节律观察

采用瞬时取样法对圈养的2对蓝鹇和2组白鹇繁殖期行为的时间节律进行了初步研究,分别选择蓝鹇和白鹇繁殖行为较为明显的时期,各记录15 d,即蓝鹇为2008年3月30日～4月15日(12、13日因故未观察),白鹇为2008年4月16～30日;每天观察12 h,即从7:00～19:00。结果表明第一组蓝鹇和白鹇的繁殖行为都明显少于第二组蓝鹇和白鹇,蓝鹇和白鹇的繁殖行为的发生次数随日期出现波动,两物种繁殖行为的高峰多在早晚时段。蓝鹇和白鹇的产蛋周期分别为3～4 d和1～3 d;两物种雌性个体在产蛋前均有较多繁殖行为发生;两物种的繁殖对配对个体日间发情的同时性要求均不高,白鹇的繁殖与配对个体繁殖行为发生的绝对频次多少也无关,蓝鹇的繁殖与配对个体繁殖行为发生绝对频次多少的关系尚不明确。     

基于PLC的设施蔬菜环境监控系统设计与应用

针对设施蔬菜环境难以把握、难以调控的问题,采用工控化思想,综合利用自动化、通讯工程等技术,设计一套基于PLC的环境监控系统,实现了设施蔬菜环境监测信息化、环境调控自动化.实践证明该系统能降低病虫害发生概率、降低水肥药投入成本和人力管理成本,是实现设施蔬菜优质、高产、高效的有效技术手段.     

一种商业银行贷款项目绩效评估新模型

国内外对商业银行经营绩效评价方法主要体现在指标体系,但在模型上并没有重视适用性,造成商业银行贷款项目进行绩效评估时缺少适合的模型.根据商业银行贷款项目进行绩效评估的特点,利用灰色关联分析法、模糊评判综合法和逐步判别分析法各自优点结合形成一种GFS新算法,对其新的贷款项目进行判别分析.实践结果表明,GFS新算法评估效果好,能为银行高层管理者在贷款项目决策中提供依据.     

Investigation on Evolutionary Relationships of the Subgenomes in Interspecific Triploid Cotton via Meiotic FISH

We report in this paper primary studies on interspecific species of cotton vis GISH (genomic in situ hybridization).We use interspecific triploid hybrids (F1 from hybridization of allotetraploid cultivated species with diploid A,D,or C genome species) and two cultivated tetraploids to study the chromosome paring during meiosis of pollen mother cellls (PMCs) and to estimate the consequences on synapsis between these three subgenomes after synthetic polyploid formation.