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11.
Sijin Hang Hui Chen Wenhui Wu Shiyi Wang Yiwen Fang Ruilong Sheng Qidong Tu Ruihua Guo 《Marine drugs》2022,20(6)
Compound 1 (SMTP-7, also FGFC1), an isoindolone alkaloid from marine fungi Starchbotrys longispora FG216 and fungi Stachybotrys microspora IFO 30018, possessed diverse bioactivities such as thrombolysis, anti-inflammatory and anti-oxidative properties, and so on. It may be widely used for the treatment of various diseases, including cerebral infarction, stroke, ischemia/reperfusion damage, acute kidney injury, etc. Especially in cerebral infarction, compound 1 could reduce hemorrhagic transformation along with thrombolytic therapy, as the traditional therapies are accompanied with bleeding risks. In the latest studies, compound 1 selectively inhibited the growth of NSCLC cells with EGFR mutation, thus demonstrating its excellent anti-cancer activity. Herein, we summarized pharmacological activities, preparation of staplabin congeners—especially compound 1—and the mechanism of compound 1, with potential therapeutic applications. 相似文献
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Nadezhda E. Ustyuzhanina Maria I. Bilan Andrey S. Dmitrenok Eugenia A. Tsvetkova Sofya P. Nikogosova Cao Thi Thuy Hang Pham Duc Thinh Dinh Thanh Trung Tran Thi Thanh Van Alexander S. Shashkov Anatolii I. Usov Nikolay E. Nifantiev 《Marine drugs》2022,20(6)
Fucosylated chondroitin sulfates (FCSs) FCS-BA and FCS-HS, as well as fucan sulfates (FSs) FS-BA-AT and FS-HS-AT were isolated from the sea cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera, respectively. Purification of the polysaccharides was carried out by anion-exchange chromatography on DEAE-Sephacel column. Structural characterization of polysaccharides was performed in terms of monosaccharide and sulfate content, as well as using a series of non-destructive NMR spectroscopic methods. Both FCSs were shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n bearing sulfated fucosyl branches at O-3 of every GlcA residue in the chain. These fucosyl residues were different in pattern of sulfation: FCS-BA contained Fuc2S4S, Fuc3S4S and Fuc4S at a ratio of 1:8:2, while FCS-HS contained these residues at a ratio of 2:2:1. Polysaccharides differed also in content of GalNAc4S6S and GalNAc4S units, the ratios being 14:1 for FCS-BA and 4:1 for FCS-HS. Both FCSs demonstrated significant anticoagulant activity in clotting time assay and potentiated inhibition of thrombin, but not of factor Xa. FS-BA-AT was shown to be a regular linear polymer of 4-linked α-L-fucopyranose 3-sulfate, the structure being confirmed by NMR spectra of desulfated polysaccharide. In spite of considerable sulfate content, FS-BA-AT was practically devoid of anticoagulant activity. FS-HS-AT cannot be purified completely from contamination of some FCS. Its structure was tentatively represented as a mixture of chains identical with FS-BA-AT and other chains built up of randomly sulfated alternating 4- and 3-linked α-L-fucopyranose residues. 相似文献
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【目的】利用套算法评定不同来源菜籽粕的鹅代谢能及营养物质利用率。【方法】选取健康、体重接近的200日龄成年公鹅30只,随机分为5组(Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组),每组6个重复,每个重复1只鹅。4种菜籽粕分别由加拿大进口油菜籽及江苏省苏州市、宿迁市、盐城市本地生产的油菜籽榨油而成。采用强饲法进行代谢试验,强饲量80 g,试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组分别以4种菜籽粕替代30%基础饲粮,试验Ⅴ组强饲基础饲料,并收集内源粪便,做内源校正。【结果】(1)4种菜籽粕中的总能(GE)、干物质(DM)、粗蛋白质(CP)、粗脂肪(EE)、粗灰分(Ash)、酸性洗涤纤维(ADF)、中性洗涤纤维(NDF)、钙(Ca)、总磷(TP)含量的平均值分别是17.48 MJ/kg、88.95%、42.22%、2.47%、7.93%、22.42%、37.81%、0.83%和1.09%。(2)鹅对4种菜籽粕的表观代谢能(AME)、真代谢能(TME)平均值分别为12.27和13.05 MJ/kg,且AME、TME存在显著的组间差异(P<0.05);鹅对4种菜籽粕CP真利用率平均值为44.84%,对DM、EE、Ash、ADF、NDF... 相似文献
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In an attempt to find the biorational pesticides, we synthesized 12 pyridinyl derivatives of podophyllotoxin (PPT) and 4′-demthylepipodophylltoxin (4′-DMEP) in this study. Their structures and the α/β substitution at C-4 were confirmed by 1H NMR, IR, MS spectral analyses and elemental analysis. The insecticidal activities were tested against fifth-instar larvae of Pieris rapae and the third-instar larvae of Cullex pipiens pallens at concentrations of 250 and 10 μg ml−1. Four derivatives of PPT, 4.1, 4.2, 4.3 and 4.5, showed higher insecticidal activities against P. rapae than PPT, while three derivatives of PPT, 4.4, 4.5 and 4.6, displayed higher mosquito larvicidal activity than PPT, with LC50 values of 1.66, 3.96 and 1.54 mg l−1, respectively. Interestingly, we also found that the pyridine ring derivatives of PPT showed delayed insecticidal activity, which is different from traditional neurotic insecticides. The results suggest that 4′-OCH3 in the PPT derivatives is essential to keep the insecticidal activity and the insecticidal activities of pyridine ring derivatives of PPT are higher than that of the derivatives of 4′-DMEP, supporting PPT has the potential to be a lead structure of semi-synthetic insecticides. 相似文献
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建立猫疱疹病毒Ⅰ型(FHV-1)PCR检测方法,应用于临床样品中FHV-1的快速检测。根据猫疱疹病毒Ⅰ型的gI基因序列设计了一对特异性引物,以疑似猫疱疹病毒Ⅰ型感染猫的眼鼻分泌物总DNA为模板,建立了PCR检测方法,对所建立的方法进行特异性、敏感性和重复性验证,并对26份临床样本进行检测。结果表明,所建立的PCR诊断方法与犬细小病毒(CPV)、犬瘟热病毒(CDV)、绵羊肺炎支原体(M.ovis)、牛支原体(M.bovis)、牛疱疹病毒Ⅰ型(BHV-1)均无交叉性反应,最低检测DNA模板浓度为3.23×10~3 copies/μL。对26份临床病例进行检测,阳性率61.54%。说明建立的FHV-1PCR检测方法具有特异性强、敏感度高、方便快捷等优点,适合于临床FHV-1感染的快速检测。 相似文献
19.
H-Y抗血清的制备及检测研究 总被引:1,自引:0,他引:1
选用8 ~12 周龄纯系的 B A L B/ C 母鼠, 用同日龄的 B A L B/ C 公鼠采取皮肤移植和脾细胞注射2 种方式免疫, 制备 H Y 抗血清, 经精子微量细胞毒性试验, 筛选抗血清。皮肤移植5 只小鼠中只有1 只产生较好的抗血清, 脾细胞注射的4 只小鼠都能产生较好的抗血清, 产生抗血清的小鼠占55 % 。抗血清与昆明鼠正常8cell 早期桑椹胚培养5 ~6 、18 和24 h 后, 退化率达433 % , 经 X2 检验差异不显著( P > 005) , 符合自然性比例。 相似文献
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