卤鸭肝肫软罐头的研制

本产品以鸭肝肫为原料，经卤制、切片、灭菌等加工工艺制成鸭肝肫软罐头，该产品开袋即食，营养丰富，风味独特。     

分布式人才管理决策支持系统的设计与实现

根据当前人才工程与现代科学人事制度需要，提出建立分布式多层人才管理决策支持系统作为管理科学化，民主化的有利保证，该系统综合利用ＷＷＷ技术、ＣＯＲＢＡ技术及ＯＬＥ／ＣＯＭ技术为广大分布用户提供分布决策支持；同时，为决策系统提供了集成一些新技术的有利手段；并且决策中定性推量和定量推理能够相辅相成协同完成多级决策技术。     

In vitro blastogenic responses of peripheral lymphocytes from marmosets to phytohemagglutinin and to autologous lymphocytes transformed by Epstein-Barr virus

White-lipped marmosets were evaluated for their cell mediated immune (CMI) response to EBV to determine the feasibility of CMI studies in marmoset models for EBV oncogenesis. The mitogen, cell concentrations, the length of incubation period and serum requirements were defined for in vitro lymphocyte stimulation tests. The level of response of each animal was dependent on the concentration of phytohemagglutinin-P (PHA-P) and was independent of cell densities employed. The rate of tritiated-thymidine incorporation by mononuclear cells due to PHA-P increased exponentially between 2–4 days. This test was reproducible for a given batch of PHA-P when the cells were cultured in the presence of 10% heat inactivated fetal bovine serum. The five white-lipped marmosets were seronegative for EBV antigens and did not show lymphocyte stimulation with EBV particles and EBV soluble antigen, but two of these animals exhibited significant stimulation with autologous lymphocytes transformed in vitro by B95-8 virus. Despite the limited amount of blood (3–4 ml) that could be obtained from each animal in a single bleeding, it was possible to perform multiple lymphocyte stimulation assays with the protocol used.     

Competence of Aedes albopictus and Culex quinquefasciatus as vector of Dirofilaria immitis after blood meal with different microfilarial density

Aedes albopictus and Culex quinquefasciatus were fed canine blood with different microfilarial density of Dirofilaria immitis ranging from 2500 to 25,000 mff/ml. Larval development in these two mosquito species did not differ significantly. Although C. quinquefasciatus ingested more microfilariae, the number of larvae which developed in A. albopictus was invariably greater than in C. quinquefasciatus. Mortality of the engorged A. albopictus was significantly greater than that of C. quinquefasciatus, and higher microfilarial density raised the mortality in both species. The vector efficiency index of A. albopictus was greater than C. quinquefasciatus at all microfilarial densities, but its survival time was much reduced. Thus, dogs with low microfilarial density are implicated as the main source for the transmission of D. immitis from dogs to mosquitoes.     

热带亚热带优质高产牧草矮象草的试验研究

10年的系统研究结果表明,矮象草具有适应性与抗逆性强、产量较高质量好、粗蛋白质含量与消化率高、叶量大利用率高、动物利用增产效果显著的特点,是适于热带亚热带地区种植的优良牧草品种.     

黑龙江野生大豆优异资源筛选、评价及利用的研究

通过近25年的评价与利用,筛选出高蛋白含量48%以上的49份;抗孢囊线虫3号种质8份;抗花叶病毒种质5份;多花荚、多分枝、抗逆性强等种质9份;双高(高油、高蛋白)66.16%的品系1份;并通过种间创新获得百粒重在6￣20g,蛋白质含量中间种质材料,丰富了大豆遗传基础。     

Study on the porcinophilic foot-and-mouth disease virus I. production and characterization of monoclonal antibodies against VP1

Monoclonal antibodies (MAbs) reported here were produced against the porcinophilic foot-and-mouth disease virus (FMDV) that caused the devastating swine disease on 1997 in Taiwan. A panel (25) of MAbs were found to react with VP1 of O/Taiwan/97 (O/97) by ELISA with various potencies. The biological identities of these VP1 reacting MAbs, such as neutralization activity, isotype and capability to distinguish between two serotype O FMDVs, O/97 and O/Taiwan/KM1/99 (O/99), were further analyzed. Eleven out of the total eighteen O/97 neutralizing MAbs were able to neutralize heterologous O/99. Eight O/97 neutralizing and five non-neutralizing MAbs could differentiate two serotype O FMDVs by immunofluorescence assay (IFA) implied that these thirteen MAbs recognized O/97 specific epitope(s). Furthermore, reactivities of the VP1 reacting MAbs with a 29 amino acids synthetic peptide (P29) representing the betaG-betaH loop of VP1 were analyzed by ELISA and fourteen were found positive. MAb clone Q10E-3 reacting strongest with VP1 and P29, neutralizing both but not differentiating two serotype O viruses suggested that the antibody binding site might involve the RGD motif and its C terminal conserved region on betaG-betaH loop. MAbs with diverse characters presented in this study were the first raised against porcinophilic FMDV. The complete set of MAbs may be used for further studies of vaccine, diagnostic methods, prophylaxis, etiological and immunological researches on FMDV.     

新型杀菌化合物靶标识别及其靶向候选药剂设计概述

新型杀菌剂的创制在农业可持续发展和克服抗药性中具有重要作用。由于杀菌剂作用机制或靶标是新杀菌剂分子设计合成和创制开发的重要基础,因此作用机制或靶标研究的滞后会阻碍新先导化合物的发现或新品种的创制。鉴于此,本文对目前杀菌剂作用靶标的识别及靶向杀菌剂的分子设计进行了概述。首先阐述了基于转录组学、蛋白质组学的研究和药物亲和层析技术在靶标识别中的应用;其次概述了多学科交叉的方法在分子靶标验证中的应用;最后以strobilurin A为先导的Qo位点抑制剂、以肉桂酸为先导的卵菌抑制剂、以萎锈灵为先导的琥珀酸脱氢酶和植物免疫激活剂等不同类型的靶向杀菌剂为例进行了举例说明。     

对拖拉机变型产品技术条件的探讨

运输型或多功能拖拉机产品从 90年代中期以来进入了一个快速发展的时期。介绍了拖拉机变型产品的发展过程和作用 ,叙述了目前该产品的技术条件、行业标准的形成过程、作用和意义 ,分析了该类产品现有行业标准存在的问题 ,并提出了拖拉机变型产品技术条件行业标准的修订建议 ,重点探讨了四轮拖拉机变型产品技术条件中的一些关键技术指标     

蒙古马血液蛋白(酶)遗传多态性及聚类分析

采用垂直平板不连续聚丙烯酰胺凝胶电泳技术对221匹蒙古马（包括乌珠穆沁马、乌审马、锡尼河马和巴尔虎马）的8种血液蛋白进行多态性检测。计算了各位点的基因型频率、等位基因频率、遗传杂合度，并通过聚类分析探讨了群体间的亲缘关系和遗传距离。结果表明：除α1-B糖蛋白（A1B）位点呈现为单态外，其余7个蛋白（酶）位点均在不同程度上表现出多态。8个蛋白（酶）位点的平均杂合度分别为：乌珠穆沁马0．3296，乌审马0．3056，锡尼河马0．3783和巴尔虎马0．3666。对Nei氏标准遗传距离进行聚类分析，发现巴尔虎马和锡尼河马之间的遗传距离最近，它们与乌珠穆沁马和乌审马的遗传距离相对较远。