难忘的起步阶段

时光似箭,日月如梭。转眼,中国饲料工业协会成立20周年了,中国饲料工业也走过了从起步到腾飞的光辉历程。1985年我开始接触这个新兴行业直到1995年退休。作为一名具体工作的行业管理工作者,我度过了从艰难创业到蓬勃发展的不平凡的十年。这其中有丰收的喜悦,也有创业的艰辛;有成功的快乐,也有阻力和冷漠带来的苦涩。回忆起步阶段的十年,酸甜苦辣,百感交集。从创业到蓬勃发展1985年,山东省政府决定全省饲料工业的统筹管理由省计委移交省经委后,我开始踏入这个新的战斗行列。省政府对饲料工业是重视的。早在1985年前就成立了全省饲料工业领导…     

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.     

Culture of Differentiated Adult Rabbit Auricular Chondrocytes

Chondrocytes dedifferentiate to a fibroblast‐like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured embedded in gels as alginate, agarose or collagen. Chondrocytes cultured in suspension on a non‐adherent surface are also known to form aggregates of differentiated cells. The knowledge of chondrocyte behavior in culture is relevant for tissue engineering purposes. In this report we describe a simple method to culture differentiated or redifferentiated rabbit auricular chondrocytes on plastic surfaces with a stable phenotype. When chondrocyte aggregates formed in suspension are next seeded on plastic surfaces, most of them attach to the plastic as round or polygonal cells, and this morphological differentiation, confirmed by the presence of type II collagen, is stable for long culture periods. We also report that the addition of aggregates to monolayer cultures of dedifferentiated chondrocytes results in their redifferentiation, as is shown by their morphological changes and the synthesis of type II collagen. Therefore, this simple method can be useful for the study of chondrocyte behavior on plastic surfaces and for redifferentiating previously proliferated chondrocytes in tissue engineering techniques. Furthermore, these results demonstrate that, in addition to culture conditions such as cell isolation method or cell‐density, chondrocyte behavior on plastic depends on the presence or absence of aggregates resulting from the dissociation process.     

Insulin induced LPL mRNA expression in THP-1 macrophage and acceleration of transferring the macrophage to foam cell

AIM:To investigate the effect of insulin on ox-LDL transferring the THP-1 cells to foam cells and influencing the LPL mRNA expression in THP-1 cells.METHODS:THP-1 cells were incubated with 50 mg/Lox-LDL and insulin at concentrations of 10 mU/L, 100 mU/L, 1 000 mU/L and 10 000 mU/L, respectively. The expression of LPL mRNA in cells was detected by RT-PCR. Lipoprotein lipase of THP-1 cells was presented by no-specific lipase staining. THP-1 cells were stained with oil red O. Accumulation of total cholesterol (TC) in THP-1 cells was determined with oxidase assay.RESULTS:In 100 mU/L、1 000 mU/L、10 000 mU/L insulin groups, LPL mRNA expression increased 2 times, the average cell perilength was longer, the percentage of positive oil red O staining cells was significant higher, the content of cholesterol in THP-1 cells was higher than in ox-LDL control (P＜0.05).CONCLUSION:Insulin accelerates transferring of THP-1 cells to foam cell with exposed to ox-LDL because LPL mRNA expression increased in the cells.     

利用游离小孢子培养育成早熟大白菜新品种‘豫新5号’

 ‘豫新5号’是采用游离小孢子培养双单倍体育种技术选育的一代杂种，该品种生育期60 d，单球质量23 kg，球形指数1.35，高抗病毒病、霜霉病和软腐病，净菜产量56.7 t·hm^-2，可兼作早熟、晚熟栽培。     

Effect of tea-polyphenols on the activation of NF-κB and the expression of TGF-β1 mRNA in THP-1 cells incubated with VLDL,LDL or ox-LDL

AIM:To investigate the effect of tea-polyphenols (TP) on the activation of NF-κB and the expression of TGF-β₁ mRNA in THP-1 cells (a human acute monocytic leukemia cell line). METHODS:THP-1 cells were incubated with the different concentrations of TP, VLDL, LDL or ox-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-κB was detected with immunohistochemistry technique, the positive index of the TGF-β₁ mRNA expression was detected by hybridization in situ, and accumulation of total cholesterol (TC) in cells incubated with 0.4-40 μg/L TP was determined with oxidase assay. RESULTS:The nuclear malposition rate of NF-κB, the positive index of the TGF-β₁ mRNA expression and TC in THP-1 cells incubated with 0.4-40 μg/L of TP were lower than those with 0 μg/L of TP in TP-V group, TP-L group and TP-O (P＜0.05). The differences of these markers in THP-1 cells incubated with more than 40 μg/L TP in TP-V group, TP-L group and TP-O were not statistically significant, compared with TP-C group (P＞0.05). CONCLUSION:TP inhibited the activation of NF-κB, the expression of TGF-β₁ mRNA and the foam cell formation in the mono-macrophage.     

藜蒿玉米螟发生与防治技术的初报

亚洲玉米螟 Ostrinia furnacalis( Guenee)属鳞翅目 ,螟蛾科害虫 ,在我省玉米上普遍发生 ,为害严重 ,是重要害虫之一。该虫在我省余干县2 0 0 1年已见零星发生为害藜蒿 ,2 0 0 2年新建县铁河乡方洲村 2 hm2 藜蒿基地 ,6月中旬见幼虫发生与为害 ,7～ 8月份在藜蒿上暴发成灾 ,1 0月份还有大量的幼虫为害 ,各虫态并存 ,以高龄幼虫为主。据笔者于 2 0 0 2年 1 0月 1 1日在铁河乡方洲村调查 ,虫株率低的为 50 % ,高的达 1 0 0 % ,少数每株有幼虫 1～ 2条 ,多数每株有幼虫 3～ 6条 ,一般可减产 2 0 %～ 3 0 %、重达 50 %以上。1　寄主作物与为害…     

晚稻稻瘟病BP神经网络分区预报

应用相关分析方法分析了浙江省19个县1988～1999年晚稻稻瘟病发病与有关环境因子的关系,筛选了8个气象因子用于晚稻稻瘟病发生程度长期预报。根据各预报因子与稻瘟病发病程度相关性,采用邻接二维图论聚类分析法,将19个点(县)划分为4个生态区。每个生态区内运用BP神经网络技术建立模型,并进行拟合和试报。1997～1999年试报验证,在划分稻瘟病生态区的基础上,应用BP神经网络模型对稻瘟病进行长期预测预报是可行的,3年试报成功率分别是78.95％、84.21％和78.95％。文中还对该方法与过去常用的预报方法的试报结果作了比较。     

日本金龟子在中国适生区的预测

日本金龟子PopilliajaponicaNewman是一种重要的害虫 ,主要危害草坪和观赏植物。日本金龟子原产日本 ,现在已经分布于许多国家 ,并且造成了严重的经济损失。目前中国尚未有该虫分布。本文通过以计算机为基础的生态气候模型—CLIMEX ,利用气候数据来预测日本金龟子适合的地理分布区 ,预测结果是日本金龟子可以在中国大约 31个市、县地区生存 ,主要是沿海地区和长江流域 ,尤其是中国的中东部地区     

转 Bt基因抗虫棉对棉大卷叶螟抗性的研究

:转Bt基因抗虫棉对棉铃虫有很好的抗性,对棉大卷叶螟(SyleptaderogataFabricius)的抗性未见报道。作者研究表明,棉大卷叶螟在棉田为聚集分布型,低龄幼虫有集中为害习性;抗虫棉对其有很好的抗性,平均虫株率为2.2%,较常规棉中棉所12降低96.6%;百株虫量为133.8头,较常规棉降低88.4%。