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141.
Monoclonal antibody-directed radioimmunoassay detects cytochrome P-450 in human placenta and lymphocytes 总被引:2,自引:0,他引:2
B J Song H V Gelboin S S Park G C Tsokos F K Friedman 《Science (New York, N.Y.)》1985,228(4698):490-492
A multiplicity of cytochromes P-450 is responsible for the detoxification and activation of xenobiotics such as drugs and carcinogens. Individual differences in sensitivity to these agents may reside in the cytochrome P-450 phenotype. A monoclonal antibody-directed radioimmunoassay was developed that detects epitope-specific cytochromes P-450 in human placentas and peripheral lymphocytes. Placentas from women who smoked cigarettes contained greater amounts of cytochrome P-450 with the monoclonal antibody-specific epitope than placentas from nonsmokers. The amount of this cytochrome P-450 in human peripheral lymphocytes increased after treatment of the mitogenized lymphocytes with the cytochrome P-450 inducer benz[a]anthracene. 相似文献
142.
Gaudi BS Bennett DP Udalski A Gould A Christie GW Maoz D Dong S McCormick J Szymanski MK Tristram PJ Nikolaev S Paczynski B Kubiak M Pietrzynski G Soszynski I Szewczyk O Ulaczyk K Wyrzykowski L;OGLE Collaboration Depoy DL Han C Kaspi S Lee CU Mallia F Natusch T Pogge RW Park BG;MuFUN Collaboration Abe F Bond IA Botzler CS Fukui A Hearnshaw JB Itow Y Kamiya K Korpela AV Kilmartin PM Lin W Masuda K Matsubara Y Motomura M Muraki Y Nakamura S Okumura T Ohnishi K Rattenbury NJ Sako T Saito T Sato S 《Science (New York, N.Y.)》2008,319(5865):927-930
Searches for extrasolar planets have uncovered an astonishing diversity of planetary systems, yet the frequency of solar system analogs remains unknown. The gravitational microlensing planet search method is potentially sensitive to multiple-planet systems containing analogs of all the solar system planets except Mercury. We report the detection of a multiple-planet system with microlensing. We identify two planets with masses of approximately 0.71 and approximately 0.27 times the mass of Jupiter and orbital separations of approximately 2.3 and approximately 4.6 astronomical units orbiting a primary star of mass approximately 0.50 solar mass at a distance of approximately 1.5 kiloparsecs. This system resembles a scaled version of our solar system in that the mass ratio, separation ratio, and equilibrium temperatures of the planets are similar to those of Jupiter and Saturn. These planets could not have been detected with other techniques; their discovery from only six confirmed microlensing planet detections suggests that solar system analogs may be common. 相似文献
143.
担子菌中牛磺酸的检测 总被引:2,自引:0,他引:2
Shim.M.J Park.T 《安徽农业大学学报》1999,26(3):302-305
利用基于离子交换色谱原理氨基酸自动分析仪检测了一些常见食用菌中牛磺酸的含量。牛磺酸在磷酸丝氨酸及磷酸乙醇胺洗脱液中经色谱分析可得到清晰的对称峰。灵芝的牛磺酸含量为每100g干重含3.7~83umol。火菇在所检蘑菇中牛磺酸含量高,每100g湿重含(83.2±6.6)umol,与陆生植物相近。双孢蘑菇与香菇的牛磺酸含量相对较高,每100g湿重含量约49~55umol,牡蛎蘑菇及糙皮侧耳的鲜重含量分别为(8.9±0.712)umol、(3.7±0.3)umol,木耳及甘露地衣的干重含量分别为(19.5±2.1)umol、(9.3±1.5)umol。高牛磺酸、无胆固醇的蘑菇有望作为低胆甾醇类食物的替代品。 相似文献
144.
Magnetospheric effects of power line radiation 总被引:1,自引:0,他引:1
Radiation from electrical power lines leaks into the magnetosphere and stimulates strong very-low-frequency wave activity out to many earth radii. Observations in Antarctica show that wave activity induced by power lines tends to occur during the daytime when power consumption is high in the source region in eastern Canada. The wave frequency ranges from 1 to 8 kilohertz. This man-made wave activity may have significant effects on energetic electrons trapped in the earth's radiation belts. 相似文献
145.
146.
Park HG Kim SH Kwon SH Ju YG Yang JK Baek JH Kim SB Lee YH 《Science (New York, N.Y.)》2004,305(5689):1444-1447
We report the experimental demonstration of an electrically driven, single-mode, low threshold current (approximately 260 microA) photonic band gap laser operating at room temperature. The electrical current pulse is injected through a sub-micrometer-sized semiconductor wire at the center of the mode with minimal degradation of the quality factor. The actual mode of interest operates in a nondegenerate monopole mode, as evidenced through the comparison of the measurement with the computation based on the actual fabricated structural parameters. As a small step toward a thresholdless laser or a single photon source, this wavelength-size photonic crystal laser may be of interest to photonic crystals, cavity quantum electrodynamics, and quantum information communities. 相似文献
147.
Sang-Don Lee Elizabeth R. Ellwood Se-young Park Richard B. Primack 《Biological conservation》2011,(9):2182-2187
Barn swallows (Hirundo rustica) are arriving later in the spring than they did 30–40 years ago at numerous sites in Korea. In some cases their arrival times are later by more than 1 month. This result is perplexing as spring activities of plants and animals are generally getting earlier due to warming temperatures. The first arrival dates of swallows are not related to temperature, suggesting that another factor is involved. On the basis of a questionnaire, a large majority of long-term observers are confident that there has been a moderate to severe decline in swallow populations at their field site over the study period. The greatest delays in arrival times are associated with sites with more severe reported declines in population size. Simulations using trapping data of large migratory bird populations from the United States, consisting of hundreds of individuals, suggest that severe population declines of 99% can result in delays of 10–12 days in arrival times. In summary, our results suggest that the large delays in arrival time of Korean swallows are due, at least in part, to severe reductions of more than 99% in what were formerly very large populations. Significant delays in spring phenology over time during a period of climatic warming may indicate population decline, though alternative explanations, such as changes in range or migration path or changing number of broods per season, should also be investigated. Delays in first arrival data can provide a valuable new tool to conservation biologists by indicating declines in a population that would otherwise go unnoticed. This can, in turn, lead to efforts by researchers to verify the dynamics of a population and draw attention to the conservation needs of the species. 相似文献
148.
Arash Minai-Tehrani Young-Chan Park Soon-Kyung Hwang Jung-Taek Kwon Seung-Hee Chang Sung-Jin Park Kyeong-Nam Yu Ji-Eun Kim Ji-Young Shin Ji-Hye Kim Bitna Kang Seong-Ho Hong Myung-Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):309-317
Conventional lung cancer therapies are associated with poor survival rates; therefore, new approaches such as gene therapy are required for treating cancer. Gene therapies for treating lung cancer patients can involve several approaches. Among these, aerosol gene delivery is a potentially more effective approach. In this study, Akt1 kinase-deficient (KD) and wild-type (WT) Akt1 were delivered to the lungs of CMV-LucR-cMyc-IRES-LucF dual reporter mice through a nose only inhalation system using glucosylated polyethylenimine and naphthalene was administrated to the mice via intraperitoneal injection. Aerosol delivery of Akt1 WT and naphthalene treatment increased protein levels of downstream substrates of Akt signaling pathway while aerosol delivery of Akt1 KD did not. Our results showed that naphthalene affected extracellular signal-regulated kinase (ERK) protein levels, ERK-related signaling, and induced Clara cell injury. However, Clara cell injury induced by naphthalene was considerably attenuated in mice exposed to Akt1 KD. Furthermore, a dual luciferase activity assay showed that aerosol delivery of Akt1 WT and naphthalene treatment enhanced cap-dependent protein translation, while reduced cap-dependent protein translation was observed after delivering Akt1 KD. These studies demonstrated that our aerosol delivery is compatible for in vivo gene delivery. 相似文献
149.
Ji Hyung Kim Sun Young Hwang Jee Soo Son Jee Eun Han Jin Woo Jun Sang Phil Shin Casiano Choresca Jr Yun Jaie Choi Yong Ho Park Se Chang Park 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):41-48
The antibiotic resistance of 16 Aeromonas (A.) salmonicida strains isolated from diseased fish and environmental samples in Korea from 2006 to 2009 were investigated in this study. Tetracycline or quinolone resistance was observed in eight and 16 of the isolates, respectively, based on the measured minimal inhibitory concentrations. Among the tetracycline-resistant strains, seven of the isolates harbored tetA gene and one isolate harbored tetE gene. Additionally, quinolone-resistance determining regions (QRDRs) consisting of the gyrA and parC genes were amplified and sequenced. Among the quinolone-resistant A. salmonicida strains, 15 harbored point mutations in the gyrA codon 83 which were responsible for the corresponding amino acid substitutions of Ser83→Arg83 or Ser83→Asn83. We detected no point mutations in other QRDRs, such as gyrA codons 87 and 92, and parC codons 80 and 84. Genetic similarity was assessed via pulsed-field gel electrophoresis, and the results indicated high clonality among the Korean antibiotic-resistant strains of A. salmonicida. 相似文献
150.
Dong-Kun Yang Young-Nam Park Gyeong-Soo Hong Hee-Kyung Kang Yoon-I Oh Soo-Dong Cho Jae-Young Song 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):57-63
The nucleoprotein (N) and glycoprotein (G) of 11 Korean rabies virus (RABV) isolates collected from animals diagnosed with rabies between 2008 and 2009 were subjected to molecular and phylogenetic analyses. Six isolates originated from domestic animals (cattle and dogs) and five were obtained from wild free-ranging raccoon dogs. The similarities in the nucleotide sequences of the N gene among all Korean isolates ranged from 98.1 to 99.8%, while those of the G gene ranged from 97.9 to 99.3%. Based on the nucleotide analysis of the N and G genes, the Korean RABV isolates were confirmed as genotype I of Lyssavirus and classified into four distinct subgroups with high similarity. Phylogenetic analysis showed that the Korean isolates were most closely related to the non-Korean NeiMeng1025B and 857r strains, which were isolated from rabid raccoon dogs in Eastern China and Russia, respectively. These findings suggest that the Korean RABV isolates originated from a rabid raccoon dog in Northeastern Asia. Genetic analysis of the Korean RABV isolates revealed no substitutions at several antigenic sites, indicating that the isolates circulating in Korea may be pathogenic in several hosts. 相似文献