Study of Semen Cryopreservation for Banna Mini-pig Inbred Line

In this study, different concentrations of glycerol were added to semen cryoprotective extenders of Banna Mini-pig inbred line (BMI), different thawing solutions and thawing methods were studied. After thawing, the motility, the rates of plasma integrity and acrosome integrity were compared to optimize the cryopreservation method of BMI semen. The results showed that the motility, the rates of plasma integrity and acrosome integrity were significantly higher in 2% and 3% glycerol groups than those in 1%, 4% and 5% glycerol groups (P<0.05);The motility, the rates of plasma integrity and acrosome integrity were significantly higher in thawing solution Ⅰ than those in thawing solution Ⅱ, Ⅲ and Ⅳ (P<0.05); Through comparing the motility, the rates of plasma integrity and acrosome integrity, the thawing effect in 40℃ 6 s and 50℃ 6 s groups were better than that in 38℃ 30 s (P<0.05). In conclusion, the optimal semen cryoprotective method for BMI was 2% or 3% glycerol as cryoprotectant, using thawing solution Ⅰ to thaw at 40℃ 6 s or 50℃ 6 s.     

民办高校图书馆进行大学生文化素质教育方式探析——基于黑龙江省民办高校图书馆的调查与分析

提高大学生的文化素质教育已成为高等教育改革面临的一项紧迫任务,民办院校的任务尤为沉重.在对哈尔滨市3所民办院校1 000名在校大学生进行问卷调查和分析的基础上,深入剖析图书馆开展大学生文化素质教育的现状,探讨如何利用大学图书馆的教育职能,充分发掘民办高校图书馆开展文化素质教育的作用和优势,通过实践摸索找到适合民办高校图书馆开展大学生文化素质教育培养的切实可行的有效途径和方式.     

农杆菌菌株AGL1抑菌剂的抑菌效果及其对纤维亚麻下胚轴不定芽分化率的影响

研究了几种抗生素及AgNO3的抑菌效果和对纤维亚麻下胚轴不定芽分化率的影响,选出抑菌抗生素头孢噻肟钠的效果最好,头孢曲松钠次之,两者的有效抑菌浓度分别为200mg/l和300mg/l,抑菌时间均超过10d,此浓度下纤维亚麻品种双亚5号下胚轴的不定芽分化率分别为93.6%和91.3%,鉴于两者的使用效果相近而头孢噻肟钠的成本较高,确定首选头孢曲松钠为农杆菌菌株AGL1的抑菌剂。同时,试验数据显示用头孢曲松钠（300mg/l）作为抑菌剂对纤维亚麻品种双亚5号、双亚7号和双亚10号进行遗传转化时,三者的不定芽分化率无明显差异。因此,头孢曲松钠适合作为这三个品种应用AGL1菌株进行遗传转化的首选抑菌剂,抑菌浓度为300mg/l。     

玉米、豆粕中胆碱生物学效价的研究

用肉仔鸡进行为期1周(8～14日龄)的生长反应试验 ,用标准曲线及斜率比法测定了玉米、豆粕中胆碱生物学效价。按单因子试验设计 ,用艾维因商品代8日龄肉仔鸡160只 ,随机分为10个处理组 ,每组4个重复、每个重复4只鸡。通过在玉米淀粉酪蛋白纯合饲粮中分别添加胆碱(由氯化胆碱提供)0、300、600和900mg/kg,作出生长反应曲线。用玉米分别占42.21 %、72.24 %的两个玉米半纯合饲粮和豆粕比例分别为16.50 %、24.83 %的两个豆粕半纯合饲粮饲喂肉仔鸡 ,测出玉米和豆粕中胆碱生物学效价分别为57.47 %和45.97 %。用两个玉米 +豆粕混合日粮作加性效应检验 ,证明玉米、豆粕中胆碱生物学效价可加性良好     

浅谈信息时代的农业科研管理

本文论述了信息化对农业科研管理工作的重要性，就目前农业科研管理工作的现状，提出信息时代农业科研管理工作的建议。     

A fine map for maternal lineage analysis by mitochondrial hypervariable region in 12 Chinese goat breeds

As the fast pace of genomic research continues to identify mitochondrial lineages in animals, it has become apparent that many independent studies are needed to support a robust phylogenetic inference. The aim of this study was thus to further characterize the maternal lineage, proposed to originate in southwestern region of China, using a wider survey of diverse goat breeds in China. To this end, we sequenced the mitochondrial hypervariable region 1 (HVR1) of the mtDNA control region in 145 goats of 12 Chinese breeds. Phylogenetic analysis revealed that Chinese goats were classified into four distinct lineages (A, B, C and D) as previously reported. A Mantel test and the analysis of Analysis of Molecular Variance (ANOVA) indicated that there was not an obvious geographic structure among Chinese goat breeds. Population expansion analysis based on mismatch distribution and Fu's Fs statistic indicate that two expansion events in Chinese goats occurred respectively at about 11 and 29 mutational time units ago, revealing two star-like subclades in lineage B corresponding to two population expansion events. Moreover, lineage B sequences were presented only in the breeds of southwestern or surrounding regions of China. Multiple lines of evidence from this study and previous studies indicate that for Chinese goats mtDNA lineage B originated from the southwestern region of China.     

6种野生草莓基因组大小估算

【目的】估算野生草苺的基因组大小。【方法】以拟南芥(Arabidopsis thaliana)为内标,利用流式细胞仪测定了6个野生草莓种7个待测样品的基因组大小。【结果】6个野生草莓种的基因组大小分别为:黄毛草莓(274±3.80)Mb,五叶草莓(红果类型)(268±3.73)Mb,五叶草莓(白果类型)(258±5.40)Mb,西南草莓(245±3.28)Mb,东北草莓(241±4.28)Mb,森林草莓(231±3.19)Mb,绿色草莓(230±2.83)Mb。【结论】野生草莓基因组大小的测定为草莓属植物基因组文库的建立及其基因组进化等研究提供了基础数据。     

外源水杨酸对盐胁迫下苦菜抗氧化系统的影响

以苦菜为试验材料,研究了5 mmol/L水杨酸（SA）预处理后,苦菜幼苗分别在0.6%、0.8%、1.0%NaCl溶液胁迫下5、10、15、20、25 d时抗氧化系统的变化。研究结果表明,5 mmol/L SA处理提高了盐胁迫下苦菜幼苗细胞SOD、POD的活性,抑制了CAT的活性;在盐胁迫处理后10、15 d,苦菜细胞内脯氨酸和H2O2含量分别达到峰值,并显著高于清水对照,说明SA通过诱导H2O2含量增加,提高抗氧化酶活力,缓解盐胁迫伤害,提高苦菜耐盐性。     

蔬菜根结线虫生防细菌的筛选及菌株HJT2-1种类的初步鉴定

以天津地区7个区县的蔬菜大棚土壤中分离出的306株细菌菌株为试材,采用亚甲基蓝染色法,研究306株细菌菌株发酵液对南方根结线虫2龄幼虫的影响。结果表明:细菌菌株HJT2-1发酵液对南方根结线虫2龄幼虫的校正死亡率较高为81.3%,其温室盆栽防效为55.6%,略低于阿维菌素防效。采用对细菌核糖体DNA的内转录间隔区进行分析的方法将菌株HJT2-1初步鉴定为假单胞菌属细菌。该试验获得了对根结线虫2龄幼虫具有较高致死率的细菌菌株HJT2-1,为开发防治根结线虫的生防制剂奠定了基础。