河北省近35年（1965—1999年）参考作物蒸散量的时空变化

在气候变化大背景下,分析河北省参考作物蒸散的时空变化趋势和影响因素,为农作物水分管理提供指导.根据FAO推荐的彭曼-蒙蒂斯方程,利用河北省1965-1999年85个地面气象站资料,计算并分析了河北省参考作物蒸散量的时空分布变化及其与气候变化的关系.结果表明:河北省春、夏、秋、冬四季和年的参考蒸散量序列变化呈现下降趋势,并达到显著水平(α=0.01).其中,春季下降最快,夏季次之,秋季较慢,冬季下降最慢;年参考蒸散量减少速率达43.58mm/10a.在空间上,全省参考作物蒸散量在不同地区减少幅度不同.其中,廊坊及以南地区下降较为突出,减少速率一般均在40mm/10a以上,而北部地区减少较为缓慢,一般均在35mm/10a以下,秦皇岛虽然有所减少,但未达到显著水平(α＞0.05).通过参考作物蒸散与气候要素相关分析表明:影响河北省参考作物蒸散变化的主要因子是风速和日照时数(即太阳辐射).根据能量平衡原理,风速减小和日照时数减少是河北省参考作物蒸散下降的主要原因,而气温升高对其影响作用不显著.     

雨季不同时段蒋家沟泥石流侵蚀产沙特征的分析

根据雨季不同时段,降雨入渗后的土壤含水状况观测以及泉水季节性变化,详细分析了蒋家沟1987-2001年雨季不同时段的167场泥石流侵蚀产沙的特点。结果表明:在雨季中期(7-8月),无论是泥石流暴发频率、产沙量还是规模都是最高值,分别占总样本的70.65%和占总产沙量的78.88%。雨季初期(5-6月)和雨季后期(9-10月)的泥石流,分别占总产沙量的16.16%和4.97%。雨季初期5月的雨沙比(R/s,mm/万m3)高达82.26,6月为3.49;而雨季中期的7月R/s仅为1.32,8月为1.67。这一研究可提高雨季不同时段泥石流降水警戒值确定的精度。     

蔬菜中克百威残留的ELISA法测定技术研究

以合成人工抗原复合物免疫新西兰大白兔,制备了特异性的兔抗BFNH多克隆抗血清。用间接ELISA法测得抗体血清的最高效价可达1:32000。并以BFNH-卵清蛋白(OVA)复合物,作为抗原包被96孔板,建立了间接竞争ELISA法,用于蔬菜中的克百威残留分析。通过实验确定该方法的工作条件和标准曲线,并测定了抗体血清与其它各种氨基甲酸酯类农药的交叉反应率。实验结果表明,抗体血清对其它氨基甲酸酯类农药的交叉反应率均小于10%。该方法对克百威纯品的检测限为0.1μg·ml-1,线性检测范围为102～10-3μg·ml-1。该方法测定结果与高效液相色谱测定结果相一致。     

格氏栲天然林主要种群直径分布结构特征

应用 5种概率分布律对格氏栲林 8个主要树木种群的直径结构进行拟合 ,比较不同类型的拟合效果 ,探索各种群直径分布结构特征 .结果表明 ,β分布函数较好表达格氏栲林主要树木种群的直径分布结构 ,拟合效果最好 ,正态分布函数次之 ;不同群落类型的格氏栲种群直径分布均符合β分布 ,偏度大部分呈正偏 ,峰度均为负 ,表明优势种格氏栲种群直径分布范围较大、中径材占多数 .同时格氏栲种群直径 β分布参数与林分平均胸径等特征因子存在显著关系 .其研究对格氏栲林的合理经营及森林调查 ,均具有重要意义     

自然植被净第一性生产力模型的改进

在Miami模型和Thornthwaite模型的基础上，采用直接搜索法改进了自然植被净第一性生产力模型，该模型优于Miami模型和Thornthwaite模型，便于估算自然植被的净第一性生产力，为合理利用气候资源，充分发挥气候生产潜力，最大限度地提高植物的产量提供了理论依据。     

CALIS与高校图书馆文献资源建设

简介CALIS概况,并就CALIS与文献资源建设关系,以及高校图书馆在CALIS网络下做好:1)以重点学科建设发展为依据,突出馆藏特色;2)明确目标,增加采集渠道;3)调整馆藏结构,合理收藏各类型出版物;4)适当引进全文数据库,实现采访人员专业化等方面措施进行较详论述.     

杉木与七种阔叶树混交造林效果的初步研究

通过对7种优良乡土阔叶树种与杉木混交林的调查研究,结果表明:杉阔混交林提高了林分蓄积量,促进杉木和阔叶树种成林、成材。混交林中的杉木普遍比杉木纯林生长好,只有杉檫混交林例外。杉木和木兰科观光木、乳源木莲、壳斗科红锥、格氏栲以及樟科的檫树、樟树混交造林,有利于发展珍贵阔叶树种种群规模,具有推广应用前景。处理好种间关系是营造混交林成败的关键。     

论食用菌菌种生产与经营法律规定

食用菌产业的发展具有极大的发展前景,对于食用菌生产也提出了越来越严格的要求。食用菌产业的发展在为农民提供了谋生的途径的同时,还需要加强食用菌菌种监督与管理,层层把关。     

Heme oxygenase-1 protects renal function in septic rats via influencing expression of thrombomodulin

AIM: To investigate the protective effect of heme oxygenase-1 (HO-1) on the kidney of septic rats and the influence of HO-1 on the expression of thrombomodulin (TM) in the kidney. METHODS: Sepsis in rats was developed with cecal ligation and puncture (CLP). The septic rats were randomly divided into sham group, CLP group, CLP+HO-1 inducer group and CLP+HO-1 inhibitor group (n=18). The plasma levels of creatinine (Cr), cystatin-C (Cys-C), carboxyhemoglobin (COHb), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and TM, and the changes of prothrombin time (PT) and activated partial thromboplastin time (APTT) in each group were measured. Histopathological examination was performed in the kidney. The expression of TM in the kidney tissue was detected by Western blot. RESULTS: Compared with sham group, significantly elevated plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), shortened PT and APTT (P<0.05), significantly increased microthrombus formation, and lowered TM expression in the kidney (P<0.05) of CLP group were observed. The administration of hemin lowered the plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), prolonged PT and APTT (P<0.05), attenuated microthrombus formation, and up-regulated the expression of TM in the kidney (P<0.05). In contrast, ZnPP had the opposite effects. CONCLUSION: HO-1 increases the expression of TM in the kidney and exerts anticoagulatory and antiinflammatory effects, thereby improving renal function in the septic rats.     

Effect of xeroderma pigmentosum group D gene on proliferation of human umbilical arterial smooth muscle cells induced by Ox-LDL

AIM: To investigate the effects of xeroderma pigmentosum group D (XPD) gene on the proliferation of human umbilical arterial smooth muscle cells (HUASMCs) induced by oxidized low-density lipoprotein (Ox-LDL). METHODS: The recombinant plasmid pEGFP-N2/XPD was transfected into HUASMCs by liposome. The cells were divided into blank control group, pEGFP-N2 group, pEGFP-N2/XPD group, Ox-LDL group, Ox-LDL+pEGFP-N2 group and Ox-LDL+pEGFP-N2/XPD group. The proliferation rate of the cells was detected by MTT and EdU assays. The apoptotic rate and cell cycle distribution were analyzed by flow cytometry. The protein levels of XPD, caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with blank control group, the expression of XPD was increased in pEGFP-N2/XPD group (P<0.05). According to the results of MTT and EdU assays, the cell proliferation in pEGFP-N2/XPD group was reduced compared with blank control group (P<0.05). Compared with Ox-LDL group, the cell proliferation in Ox-LDL+pEGFP-N2/XPD group was significantly inhibited (P<0.05). According to the results of flow cytometry, the cell proportion of S phase decreased and the G₀/G₁-phase cell proportion increased significantly in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group compared with blank control group and Ox-LDL group, repectively (P<0.05). Compared with blank control group and Ox-LDL group, the protein level of Bcl-2 decreased and the protein levels of Bax and cleaved caspase-3 increased in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group, respectively (P<0.05). CONCLUSION: XPD inhibits the proliferation of HUASMCs and promotes their apoptosis, and reduces the promoting effect of Ox-LDL on the proliferation of HUVSMCs. XPD may be the target for treatment of atherosclerosis.