电机护环钢在硝酸盐溶液中应力腐蚀开裂的控制过程研究

本文在文献［１］基础上，运用金相，电化学方法研究了５０Ｍｎ１８Ｃｒ４电机护环钢依照ＱＨＪ－７９试验标准在硝酸盐介质中应力腐蚀开裂（即ＳＣＣ）的性质，结果指出，此体系中ＳＣＣ的控制过程为阳极溶解。     

抗绵羊进行性肺炎病毒单克隆抗体杂交瘤细胞株的建立

用提纯的OPPV细胞抗原免疫BALB/c小鼠脾脏与SP2/0小鼠骨髓细胞融合,融合率为70%,以ELISA双抗体夹心法进行检测,阳性率为20%,经过有限稀释法克隆出1A₆、2B₈、1G₃、2G₄4株分泌抗OPPV抗原的单克隆体杂交瘤细胞株.选择抗体分泌滴度高的1A₆细胞进行了较详细的研究,结果表明,其核内染色体数为96～110条,抗体属性为IgG1,轻链为K链,IFA试验表明产生的McAb对OPPV有特异性,细胞传30代并在液氮中保存半年后复苏仍能稳定地分泌抗OPPV McAb,细胞分泌抗体的ELISA滴度腹水为6.4×10⁴,上清为640,McAb经硫基乙醇还原后在SDS-PAG上呈现2条区带,一条为重链部分,MW50000,另一条为轻链部分,MW为25000.     

Insertion of a transtympanic ventilation tube for the treatment of otitis media with effusion

In this paper, a case of otitis media with effusion (‘glue ear’) is described in a Cavalier King Charles spaniel. Its presentation, diagnosis and surgical management by the insertion of a trans-tympanic ventilation tube (grommet) is discussed.     

Genetics of Body Color in Tilapia mossambica

Gold, bronze, and black (normal pigmentation) body colors in Tilapia mossambica are controlled by a single autosomal gene with incomplete dominant gene action: GG fish are black gg fish are gold; Gg fish are bronze. Because gold body color is produced by the recessive genotype, it is easy to produce and to maintain a truebreeding population of gold T. mossambica for commercial purposes. If all other colors are culled, the population will breed true, because gold × gold will always produce 100% gold offspring. The G gene will be a valuable genetic marker for many genetic studies, such as the production of gynogenetic T. mossambica .     

Cellular injury and lipid peroxidation induced by hexavalent chromium in isolated rat hepatocytes

In order to elucidate the relationship between cellular injury and lipid peroxidation induced by hexavalent chromium (CrVI), isolated rat hepatocytes treated with any one of scavengers of active oxygen species, antioxidants or antichromium agent were incubated with K2Cr2O7 as CrVI (1 mM Cr). After the incubation, the development of lipid peroxidation was determined as thiobarbituric acid (TBA)-reacting materials in total lipid extracts from the incubated hepatocytes. Cellular injury was observed as a leakage of lactate dehydrogenase (LDH) from hepatocytes into incubation medium. The contents of reduced glutathione (GSH) in hepatocytes were also assessed. Results obtained were as follows: (1) CrVI facilitated lipid peroxidation in isolated hepatocytes after 20 min of incubation. On the other hand, the cellular injury induced by CrVI was barely observed even after 60 min of incubation. (2) The CrVI-induced lipid peroxidation was inhibited by catalase and mannitol as scavengers of active oxygen species, or N,N'-diphenyl-p-phenylenediamine and alpha-tocopherol as antioxidants. However the cytotoxicity of CrVI could not be prevented by these chemicals. (3) CrVI depleted the contents of intracellular GSH and diminished the activities of glutathione reductase (GR) and glutathione-S-transferase (GST) except glutathione peroxidase. (4) The scavengers of active oxygen species and the antioxidants could not prevent the depletion of intracellular GSH induced by CrVI. (6) Ascorbic acid, antichromium agent, prevented all of the lipid peroxidation, the cellular injury and intracellular GSH depletion induced by CrVI.(ABSTRACT TRUNCATED AT 250 WORDS)     

Reproductive performance of three fat-tailed breeds of sheep mated to native,Corriedale and Targhee rams

Summary Reproduction and lamb production of three fat-tailed, carpet-wool Iranian breeds of sheep; Karakul (130 ewes), Mehraban (103) and Baluchi (119) were studied. The ewes were mated either as purebreds or with Corriedale or Targhee rams. The overall least-squares means for ewes mated (showed oestrus) or ewes exposed, ewes lambed or ewes mated, lambs born of ewes lambing (prolificacy), lambs born alive of total lambs born, lambs weaned of live lambs born, lambs weaned (75 days of age) of ewes mated, kg lamb weaned per ewe mated and kg lamb weaned per 100 kg ewe mated were 98.3%, 93.5%, 103.1%, 94.9%, 97.8%, 90.0%, 18.2 kg and 38.4 kg respectively. The native breeds were not different for any of the traits studied except for prolificacy which was higher in Mehraban than in Karakul. Purebred matings did not differ from the crossbred matings for any of the traits concerning the number of lambs but crossbreeding improved kg lamb weaned per ewe mated (9.2%, P=0.08) and kg lamb weaned per 100 kg ewe mated (10.0%, P<0.05). Among the three native breeds Karakul showed the largest improvement when crossed with the exotic rams for the traits concerning kg lambs weaned. Corriedale and Targhee rams had similar effects on all the traits studied. A lower percentage of virgin ewes showed oestrus, had a lower twinning rate and productivity compared with the mature ones. Sex was not a significant source of variation in lamb livability.

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Desempeño Reproductivo De Tres Razas Cola Gruesa De Ovejas Apareadas Con Moruecos Nativos, Corridale Y Targhee

Resumen Se estudiaron la reproducción y producción de corderos iraníes de tres razas fat-tailed (Cola gruesa o grasosa) productores de lana para carpetas: Karakul (130 ovejas), Mehraban (103) y Baluchi (119). Las ovejas fueron apareadas como puras, o con moruecos Corridale o Targhee. Las medias mínimas cuadradas para ovejas apareadas (que mostraron estro) de ovejas expuestas, ovejas paridas de ovejas apareadas, corderos nacidos de ovejas paridas (prolificidad), corderos nacidos vivos del total de corderos nacidos, corderos destetados de corderos nacidos vivos, corderos destetados (75 días de edad) de ovejas apareadas, kg de cordero destetado por oveja apareada y kg de cordero destetado por 100 kg de oveja apareada, fueron: 98.3%, 93.5%, 103.1%, 94.9%, 97.8%, 90.0%, 18.2 kg y 38.4 kg respectivamente. Las razas nativas no fueron diferentes en ninguna de las características estudiadas, excepto por le prolificidad la cual fue mayor en Mehraban, que en Karakul. El apareamiento de razas puras, no difirió de los apareamientos cruzados para cualquiera de las características estudiadas concernientes con el número de corderos, pero el cruzamiento mejoró los kg. de cordero destetado por oveja apareada (9.2%,p<0.08) y kg de cordero destetado por 100 kg de oveja apareada (10.0%,P<0.05). De las tres razas nativas, la Karakul fue superior cuando se cruzó con las razas exóticas en lo concerniente a las características kg de cordero destetado. Las razas Corriedale y Targhee tuvieron efectos similares en todas las características estudiadas. Un bajo porcentaje de ovejas vírgenes ciclaron regularmente, tuvieron un porcentaje bajo de mellizos y productividad en general, comparada con las ovejas maduras. El sexo no fue una fuente significativa de variabilidad, en la supervivencia de los corderos.

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Performances De Reproduction De Trois Races De Moutons À Queue Grasse Accouplées À Des Locales, Corriedale Et Targhee

Résumé La reproduction et la production d'agneaux de 3 races iranienes à queue grasse élevées pour la production de laine à tapis: karakul (130 brebis), Mehraban (103) et Baluchi (119) furent étudiées; Les brebis furent accouplées soit en race pure soit avec des béliers Corriedale ou Targhee; Les moyennes estimées par la méthode des moindres carrés de l'ensemble des brebis accouplées (oestrus visible) par rapport aux brebis exposées; des brebis agnelant par rapport aux brebis accouplées, des agneaux nés par rapport aux brebis agnelant (prolificité); des agneaux nés vivants sur les nés totaux, des agneaux sevrés sur les nés vivants, des agneaux sevrés par brebis accouplée, et du kg d'agneau sevré par 100 kg de brebis accouplées étaient 98,3%; 93,5%, 103,1%, 94,9%, 97,8%, 90,0%, 18; 2 kg et 38,4 kg respectivement. Les races natives n'étaient pas différentes pour les autres caractères étudiés excepté pour la prolificité qui était plus élevée en Mehraban qu'en Karakul. Les accouplements en race pure ne différaient pas des croisements pour tous les caractères concernant le nombre d'agneaux mais le croisement améliorait les kg d'agneaux sevrés par 100 kg de brebis accouplée (9,2%P=0,08) et les kg sevrés par 100 kg de brebis accouplée (10,0%P<0,05). Parmi les 3 races natives, la Karakul montrait l'amélioration la plus grande lorsque croisée avec les béliers exotiques pour les caractères concernant les kg d'agneaux sevrés. Les béliers Corriedale et Targhee avaient des effets similaires sur tous les caractères étudiés. Un plus faible pourcentage de brebis vierges à oestrus visible, avait un plus faible taux de jumeaux et de productivité comparé aux brebis matures. Le sexe n'était pas une source significative de variation dans la viabilité des agneaux.

     

白枕鹤精液冷冻技术的研究

白枕鹤（ＧＲＵＳＶ　ＩＰＩＯ）羽毛青灰，红面，体型健美优雅，具有很高的观赏价值，已被国家列为二级保护动物。对白枕鹤类的人工授精、孵化、育雏以及冷冻精液技术的研究，可促进人工饲养条件下鹤类的增殖和种群的繁衍。本研究采用Ｂｅｌｔｓｖｉｌｌｅ稀释液做为基础液，４—６％ＤＭＳＯ做为冷冻保护剂，按常规冷冻法冻精配种受胎率达４０％，此项研究填补了我国在鹤类精液冷冻技术方面的空白。