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71.
Characterization of field‐evolved resistance to Bacillus thuringiensis‐derived Cry1F δ‐endotoxin in Spodoptera frugiperda populations from Argentina
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Desmi I Chandrasena Ana M Signorini Gustavo Abratti Nicholas P Storer Magdalena L Olaciregui Analiza P Alves Clinton D Pilcher 《Pest management science》2018,74(3):746-754
BACKGROUND
Transgenic maize (Zea mays L.) event TC1507 (Herculex® I insect protection), expressing Cry1F δ‐endotoxin derived from Bacillus thuringiensis var. aizawai, was commercialized in 2003 in the Americas. Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) susceptibility to Cry1F was monitored annually across several regions in Argentina using diagnostic concentration bioassays. Reduced performance of TC1507 maize against S. frugiperda was reported in 2013. A resistant population was established in the laboratory and the dominance of Cry1F resistance was characterized.RESULTS
During 2012–2015, high‐survivorship of several populations was observed in the resistance monitoring program. Reciprocal crosses of a Cry1F‐resistant population with a Cry1F‐susceptible population were evaluated to calculate effective dominance (DML) based on mortality levels observed at 100 µg/ml Cry1F. Two additional dominance levels (DLC and DEC) were calculated using lethal (LC50) or effective concentration (EC50) derived from concentration–response bioassays. Estimates indicated that Cry1F resistance in S. frugiperda in Argentina was either highly recessive (DML = 0.005) or incompletely recessive (DLC < 0.26 and DEC < 0.19).CONCLUSION
This study is the first documented confirmation and characterization of S. frugiperda Cry1F field‐evolved resistance in Argentina. The resistance to Cry1F in S. frugiperda populations collected in Argentina, is autosomal and incompletely recessive similar to the resistance reported in Brazil. © 2017 The Authors. Pest Management Science published by John Wiley © Sons Ltd on behalf of Society of Chemical Industry. 相似文献72.
73.
Ghasem Ali Parad Masoud Tabari Kouchaksaraei Gustavo Gabriel Striker Seyed Ehsan Sadati Kazem Nourmohammadi 《Scandinavian Journal of Forest Research》2016,31(5):458-466
Tolerance to flooding is crucial when thinking in promissory species for restoration of ecosystems prone to suffer soil water excess. In this study, we tested the flooding tolerance of two-years-old seedlings of Chestnut-leaved oak (Quercus castaneifolia C.A.Mey.) to determine whether it can be recommended for use in wetland restoration programs. Seedlings of Q. castaneifolia were subjected to three treatments: (1) control (C), (2) flooding for 60 days followed by a 42-day recovery period (F?+?R) and (3) continuous flooding for 102 days (F). Physiological performance, plant morphological changes and biomass accumulation were assessed. Results showed that, although net photosynthetic rates, stomatal conductance and transpiration decreased with prolonged flooding, when flood waters were removed, plants were able to recover their physiological activity (49–80% compared to controls). By contrast, when plants were continually flooded, their physiological activity decreased as well as the leaves experienced precocious senescence and wilting. Biomass responses paralleled physiological responses: leaf and root biomass were 42–49% higher under F?+?R treatment than under continuous flooding, and all plants under the F?+?R survived. Therefore, Q. castaneifolia appears as a promising species to be further studied when thinking re-vegetation of riverine areas and other temporarily flooded wetlands. 相似文献
74.
Carlos Gustavo Tornquist Joo Mielniczuk Carlos Eduardo Pellegrino Cerri 《Soil & Tillage Research》2009,105(1):33-43
Introduction of conservation practices in degraded agricultural land will generally recuperate soil quality, especially by increasing soil organic matter. This aspect of soil organic C (SOC) dynamics under distinct cropping and management systems can be conveniently analyzed with ecosystem models such as the Century Model. In this study, Century was used to simulate SOC stocks in farm fields of the Ibirubá region of north central Rio Grande do Sul state in Southern Brazil. The region, where soils are predominantly Oxisols, was originally covered with subtropical woodlands and grasslands. SOC dynamics was simulated with a general scenario developed with historical data on soil management and cropping systems beginning with the onset of agriculture in 1900. From 1993 to 2050, two contrasting scenarios based on no-tillage soil management were established: the “status quo” scenario, with crops and agricultural inputs as currently practiced in the region and the “high biomass” scenario with increased frequency of corn in the cropping system, resulting in about 80% higher biomass addition to soils. Century simulations were in close agreement with SOC stocks measured in 2005 in the Oxisols with finer texture surface horizon originally under woodlands. However, simulations in the Oxisols with loamy surface horizon under woodlands and in the grassland soils were not as accurate. SOC stock decreased from 44% to 50% in fields originally under woodland and from 20% to 27% in fields under grasslands with the introduction of intensive annual grain crops with intensive tillage and harrowing operations. The adoption of conservation practices in the 1980s led to a stabilization of SOC stocks followed by a partial recovery of native stocks. Simulations to 2050 indicate that maintaining “status quo” would allow SOC stocks to recover from 81% to 86% of the native stocks under woodland and from 80% to 91% of the native stocks under grasslands. Adoption of a “high biomass” scenario would result in stocks from 75% to 95% of the original stocks under woodlands and from 89% to 102% in the grasslands by 2050. These simulations outcomes underline the importance of cropping system yielding higher biomass to further increase SOC content in these Oxisols. This application of the Century Model could reproduce general trends of SOC loss and recovery in the Oxisols of the Ibirubá region. Additional calibration and validation should be conducted before extensive usage of Century as a support tool for soil carbon sequestration projects in this and other regions can be recommended. 相似文献
75.
Gustavo A. Ramírez Jaume Altimira Beatriz García Marcos Fernández† Miquel Vilafranca 《Veterinary dermatology》2009,20(1):35-41
Straelensiosis is a relatively new disease described in dogs and produced by a trombiculid larva known as Straelensia cynotis . Few cases have been reported in the English literature. Straelensiosis has been observed in Southern France and Northern Portugal causing a distinctive nodular dermatitis. The present study describes the clinical, histopathological and epidemiological features of 19 cases diagnosed as straelensiosis in the north of Spain and Portugal (2003–2007). A follow-up of the animals after diagnosis was carried out. Differences with previously described cases were observed, especially concerning epidemiology and histopathology. The grade of response to different treatments and new microscopic features are discussed. The study concludes that S. cynotis can cause a nodular dermatitis in rural and hunting dogs in Spain and Portugal and is associated with particular seasonal and geographical factors. 相似文献
76.
Ieda D.P. Blanco DVM MSc Liane G. Devito DVM MSc Heder N. Ferreira DVM MSc Gustavo H.M. Araujo DVM MSc Claudia B. Fernandes DVM PhD Marco A. Alvarenga DVM PhD Fernanda C. Landim-Alvarenga DVM PhD 《Journal of Equine Veterinary Science》2009,29(8):613-617
This study examined the effect of treating mares with equine pituitary extract (EPE) in combination with human chorionic gonadotropin (hCG; EPE/hCG) on the recovery rate of immature oocytes by ovum pick-up (OPU) and on oocyte morphology. Ten mares were subjected to each of two treatments in a random sequence: superstimulated with EPE (25 mg, twice daily) and treated with hCG (2,500 IU) or control (no exogenous treatment). The cytoplasmic morphology of oocytes recovered was evaluated through transmission electron microscopy. Follicular fluid was collected at aspiration for progesterone analysis, which was performed by radioimmunoassay. The EPE/hCG did not increase the oocyte recovery rate from immature follicles when compared with the controls (15.5% and 16.7%, respectively). A significantly higher oocyte recovery rate per mare was observed (70% versus 50%). However, precocious granulosa cell expansion was observed with EPE/hCG treatment in contrast to the control (64.4% and 33% of follicles with expanded cumulus, respectively), and increased intrafollicular progesterone concentration was also seen (158.80 ng/mL versus 82.05 ng/mL). The ultrastructural analysis of oocytes from both groups showed morphologic features related to immaturity. Numerous vesicles containing cortical granules were found, distributed in clusters into the cytoplasm, and junctional complexes were still seen between oocyte and granulosa cells. In conclusion, EPE/hCG treatment induced some follicular modifications, but the recovery rate was not increased. All oocytes examined presented signs of immaturity. 相似文献
77.
Baroni MV Nores ML Díaz Mdel P Chiabrando GA Fassano JP Costa C Wunderlin DA 《Journal of agricultural and food chemistry》2006,54(19):7235-7241
We report the evaluation of the floral origin of honey by analysis of its volatile organic compounds (VOCs) profile, joined with the use of combined pattern recognition techniques. Honey samples, from five floral origins, were analyzed by headspace solid-phase microextraction-gas chromatography-mass spectrometry, selecting 35 VOCs out of the entire profiles, which were analyzed by hierarchical cluster analysis (HCA), stepwise discriminant analysis (SDA), and K-nearest-neighbor (KNN). Both HCA and SDA were used as exploratory tools to select a group of VOCs representing similitude and differences among studied origins. Thus, six out of 35 VOCs were selected, verifying their discriminating power by KNN, which afforded 93% correct classification. Therefore, we drastically reduced the amount of compounds under consideration but kept a good differentiation between floral origins. Selected compounds were identified as octanal, benzeneacetaldehyde, 1-octanol, 2-methoxyphenol, nonanal, and 2-H-1-benzopyran-2-one. The analysis of VOC profiles, coupled to HCA, SDA, and KNN, provides a feasible alternative to evaluate the botanical source of honey. 相似文献
78.
Guimes Rodrigues Filho Sabrina Dias RibeiroCarla da Silva Meireles Leandro Gustavo da SilvaReinaldo Ruggiero Moacir Fernandes Ferreira JuniorDaniel Alves Cerqueira Rosana Maria Nascimento de AssunçãoMara Zeni Patricia Polleto 《Industrial Crops and Products》2011,33(3):566-571
Cellulose acetate is one of the components employed in drug controlled-release systems in the form of membranes. The aim of this study was to examine the controlled-release of doxycycline employing cellulose acetate symmetric and asymmetric membranes as matrices. The cellulose triacetate was produced from sugarcane bagasse through a homogeneous acetylation reaction, using acetic acid as the solvent, acetic anhydride as the acetylating agent and sulfuric acid as the catalyst. The viscosity average molecular weight of the cellulose acetate produced was 39,000 g mol−1. The symmetric membranes were produced using a system solvent of dichloromethane/ethanol (9:1, v/v) and the asymmetric membranes were produced from the same solvent system and 10% of water. For the formulation of both, 5% of doxycycline was used. The membranes were characterized by thermal analysis (DSC and TGA) and scanning electron microscopy SEM. The release of doxycycline through cellulose triacetate matrices was examined using spectrophotometric analysis in the ultraviolet-visible region, at 275 nm. The results revealed that asymmetric membranes release 80% of the drug in 100 min, while symmetric membranes release 14% of the drug during the same time interval. 相似文献
79.
Henry Zúñiga-Benítez Román Sánchez-Monsalve Gustavo A. Peñuela 《Water, air, and soil pollution》2018,229(10):332
This paper presents the main results obtained using the heterogeneous photocatalysis with TiO2 technology in the removal of the emerging contaminant benzophenone-3 at pilot plant scale in a cylinder parabolic solar collector. In this sense, the effects of the operational parameters, pH and catalyst concentration, were analyzed, and after the use of the response surface methodology, the conditions that, under the experimental range, conduct to a higher removal of the analyte were selected. Additionally, analysis of the total organic carbon content and the biodegradability index (relationship between BDO5 and COD) of the treated samples were carried out. In general, results allowed us to infer that this kind of treatment is effective in removing a considerable percentage of the substrate, and that it could become an effective alternative to water treatment and decontamination. 相似文献
80.
Synthesis,theoretical studies,and effect on the photosynthetic electron transport of trifluoromethyl arylamides
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