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171.
Genome‐Wide Identification of MicroRNAs Responsive to High Temperature in Rice (Oryza sativa) by High‐Throughput Deep Sequencing 下载免费PDF全文
J. Li L.‐Q. Wu W.‐Y. Zheng R.‐F. Wang L.‐X. Yang 《Journal of Agronomy and Crop Science》2015,201(5):379-388
MicroRNAs (miRNAs) are known to play important roles in plant growth and stress response. Heat stress is a severe abiotic stresses by adversely affecting plant growth and yield. To identify heat‐responsive miRNAs at the genome‐wide level in rice (Oryza sativa), we constructed two small RNA libraries from young panicles treated or not with heat conditions. Ion torrent sequencing of the two libraries identified 294 known miRNAs and 539 novel miRNAs. Differential expression analysis showed that 26 miRNAs were downregulated and 21 miRNAs were upregulated in response to heat stress. Among them, five heat‐responsive miRNAs, including miR162b, miR529a‐p5, PC‐5P‐62245‐9, miR171b and miR169n, were validated by quantitative real‐time polymerase chain reaction. A total of 44 target genes of the differentially expressed miRNAs were predicted. These target genes are most significantly overrepresented in the cell growth process. The results demonstrated that rice miRNAs play critical roles in the heat stress response. This study opens up a new avenue for understanding the regulatory mechanisms of miRNAs involvement in the heat stress response in rice. 相似文献
172.
Quinoa is a potential new seed crop for protein feed and human consumption in Europe, with tolerance to a range of abiotic stresses. For this purpose the study was planned to analyse the effect of important agronomic strategies like nitrogen level, N application strategy, row spacing and harvest time on yield and quality of quinoa. The experiments took place in the field of the experimental station of the Faculty of Science, University of Copenhagen. Three levels of organic nitrogen from slurry was used (60, 120 and 180 kg N ha?1), supplied either all at once at sowing, or split between sowing and beginning of the reproductive phase. The effect of row spacing and harvest time was studied by harvesting seeds at seed maturity, which occurred 2–3 weeks prior to the mechanical harvest by threshing, and a couple of months after. Yield increased significantly (P ≤ 0.05) with an application up to 180 kg N ha?1, reaching 2200 kg ha?1. Increasing N also caused a significantly increased seed weight (up to 3.3 mg) and protein content (up to 17 %). N level did not affect number and amount of weeds. Split application with part of the N applied at bud formation did not have a significant effect on yield. Delayed harvest had a negative influence on seed weight, whereas protein content was stable after harvesting even a month after seed maturity. A late harvest significantly reduced seed germination, being reduced by 50 % after a 2‐month delay. A conclusion from this study is that both yield and protein content of seed can be manipulated by N level and application strategy. Harvest time is important for securing a high seed quality measured as seed germination, seed weight and protein content. A fast germination of quinoa is an important characteristic demonstrating that the crop has good possibilities for being well‐established in the field when free from weeds at the time of sowing. The choice of row spacing is important and depends on weed control method. Weed control strategy should be developed based on modern precision tools. 相似文献
173.
Morphological and Physiological Responses of Plantain (Plantago lanceolata) and Chicory (Cichorium intybus) to Water Stress and Defoliation Frequency 下载免费PDF全文
L. M. Cranston P. R. Kenyon S. T. Morris N. Lopez‐Villalobos P. D. Kemp 《Journal of Agronomy and Crop Science》2016,202(1):13-24
Plants are often subjected to periods of water stress. There are little data examining the effect of water stress on the forage species Plantago lanceolata and Cichorium intybus. In two pot experiments with P. lanceolata and C. intybus, morphological responses under optimum, dry, and very‐dry water treatments with weekly, fortnightly and 3‐weekly defoliation intervals and physiological responses under optimum and very‐dry water treatments were measured. A third experiment compared the rooting depths of P. lanceolata and C. intybus under field conditions. These findings suggest that both P. lanceolata and C. intybus can survive and continue to grow under water stress conditions with the main differences between the two species being attributable to morphological characteristics (root mass, taproot diameter and shoot mass fraction) rather than differences at a physiological level. Overall, the results suggest plantain may be more productive under moderate drought due to its greater shoot mass fraction, whereas chicory may be more productive and persistent under severe drought due to its greater root mass, taproot diameter and root depth under field conditions. 相似文献
174.
针对油罐内浮盘密封失效问题逐渐凸显的现状,分析了内浮盘静、动密封与油气空间的关系,总结了油罐内浮盘密封失效的主要表现,静密封失效包括:内浮盘盖板搭接面间出现缝隙孔洞,密封胶囊或舌形密封带与内浮盘外边缘间的挟紧部位出现缝隙,防旋导管与内浮盘盖板间不密封,浮盘自动通气孔、人孔与盖板间不密封;动密封失效包括:密封胶囊或舌形密封带与罐内壁间密封性能差,量油导管与量油导管护筒盖间的密封性能差,浮盘人孔盖与人孔座间的密封性能差,防旋转钢丝绳松紧度不满足浮盘安全运行要求。基于设计、施工、使用、检修4个环节分析归纳了内浮盘密封失效的原因,提出了优化设计、控制施工质量、控制内浮盘制造安装质量、内浮盘定期检测与管理、规范检修作业等预防措施。 相似文献
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667m2用15.00%安打SC16mL和3.00%阿维菌素EC100mL处理,对水稻高龄纵卷叶螟杀虫效果最好,其防效在97.00%以上。 相似文献
177.
178.
以郑州市公园绿地、附属绿地中的120个人工植物群落为研究对象,调查其群落结构和物种多样性,利用Margalef丰富度指数、Shannon-Wiener多样性指数、Simpson多样性指数和Pielou均匀度指数对郑州市城市绿地人工群落的物种多样性进行研究,结果表明郑州市城市绿地人工群落的丰富度指数均处于较高水平,植物多样性丰富,其中公园绿地丰富度指数最高,道路附属绿地次之,社区附属绿地最低。 相似文献
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180.