Structure of the protease domain of memapsin 2 (beta-secretase) complexed with inhibitor

Memapsin 2 (beta-secretase) is a membrane-associated aspartic protease involved in the production of beta-amyloid peptide in Alzheimer's disease and is a major target for drug design. We determined the crystal structure of the protease domain of human memapsin 2 complexed to an eight-residue inhibitor at 1.9 angstrom resolution. The active site of memapsin 2 is more open and less hydrophobic than that of other human aspartic proteases. The subsite locations from S4 to S2' are well defined. A kink of the inhibitor chain at P2' and the change of chain direction of P3' and P4' may be mimicked to provide inhibitor selectivity.     

Development of an agricultural crops spectral library and classification of crops at cultivar level using hyperspectral data

In the context of a growing interest in remote sensing for precision agriculture applications, the utility of space-borne hyperspectral imaging for the development of a crop-specific spectral library and automatic identification and classification of three cultivars for each of rice (Oryza sativa L.), chilli (Capsicum annuum L.), sugarcane (Saccharum officinarum L.) and cotton (Gossipium hirsutum L.) crops have been investigated in this study. The classification of crops at cultivar level using two spectral libraries developed using hyperspectral reflectance data at canopy scale (in-situ hyperspectral measurements) and at pixel scale (Hyperion data) has shown promising results with 86.5 and 88.8% overall classification accuracy, respectively. This observation highlights the possible integration of in-situ hyperspectral measurements with space-borne hyperspectral remote sensing data for automatic identification and discrimination of various crop cultivars. However, considerable spectral similarity is observed between cultivars of rice and sugarcane crops which may pose problems in the accurate identification of various crop cultivars.     

Ameliorative influence of sesame lignans on lipid profile and lipid peroxidation in induced diabetic rats

Sesame lignans are working as antioxidants in various physiological functions. In the present study, the antioxidative effect of sesame lignans is examined in chemically induced diabetes mellitus (DM) in rats against lipid profile and lipid peroxidations. DM was induced in four groups of rats by injection of alloxan. The control groups (non-diabetic and diabetic) received a diet containing sunflower oil while the rest of the three experimental diabetic groups received a diet containing 0.25% alpha-tocopherol (D-Toc), 0.5% sesame lignan (D-SL), and 0.25% alpha-tocopherol+0.25% sesame lignan (D-Toc-SL) in sunflower oil for 4 weeks. Lipid profile and lipid peroxidations of plasma, erythrocyte membrane (EM), and liver tissues were measured. The total cholesterol, non-HDL cholesterol, plasma lipid peroxidation, and also LDL-peroxidation decreased, and HDL cholesterol increased significantly (P<0.05) in all the experimental groups as compared to the control diabetic sunflower oil group. The triacylglycerol (TAG) level in plasma decreased significantly in the D-SL and D-Toc-SL groups as compared to control diabetic group. Significant decrease in TAG level was observed in the D-SL group as compared to the D-Toc group. LDL peroxidation also decreased significantly in the D-Toc-SL group as compared to the D-Toc group. EM lipid peroxidation and liver lipid peroxidation decreased significantly in the D-Toc, D-SL, and D-Toc-SL groups as compared to the control diabetic group. Liver TAG level decreased more significantly in the D-SL and D-Toc-SL groups than in the control diabetic group. So, sesame lignans at 0.5% level and sesame lignan + alpha-tocopherol significantly ameliorate the alteration in lipid profile and the adverse free radical generative influence of DM induced by alloxan.     

Effect of Compost and Hydroabsorbent Polymer on Tree Growth and Soil Properties in a Tropical Urban Environment

Horticultural application of hydroabsorbent polymer (HP) has drawn research attention due to its perceived benefits to plant growth. Few studies have compared the use of compost and HP amendments on tree establishment in tropical urban environments. An experiment was conducted to assess the effect of compost (40% v v^–1) and HP (3 and 5 kg m^–3) on the growth of two native tree saplings (Calophyllum soulattri and Syzygium myrtifolium) in loamy and sandy soils. The HP treatments significantly affected soil pH and extractable phosphorus (P) and potassium (K), whereas combined application of compost and HP (5 kg m^–3) resulted in significantly higher nitrogen (N) in both soil types. Plant diameter and height were significantly higher following HP application. This experiment demonstrates the efficacy of HP that can cater the plant requirements at the initial growth stages which are crucial for their successful establishment in tropic urban degraded soils.     

Water transmission characteristics of a Vertisol and water use efficiency of rainfed soybean (Glycine max (L.) Merr.) under subsoiling and manuring

In Vertisols of central India erratic rainfall and prevalence of drought during crop growth, low infiltration rates and the consequent ponding of water at the surface during the critical growth stages are suggested as possible reasons responsible for poor yields (<1 t ha⁻¹) of soybean (Glycine max (L.) Merr.). Ameliorative tillage practices particularly deep tillage (subsoiling with chisel plough) can improve the water storage of soil by facilitating infiltration, which may help in minimizing water stress in this type of soil. In a 3-year field experiment (2000–2002) carried out in a Vertisol during wet seasons at Bhopal, Madhya Pradesh, India, we determined infiltration rate, root length and mass densities, water use efficiency and productivity of rainfed soybean under three tillage treatments consisting of conventional tillage (two tillage by sweep cultivator for topsoil tillage) (S₁), conventional tillage + subsoiling in alternate years using chisel plough (S₂), and conventional tillage + subsoiling in every year (S₃) as main plot. The subplot consisted of three nutrient treatments, viz., 0% NPK (N₀), 100% NPK (N₁) and 100% NPK + farmyard manure (FYM) at 4 t ha⁻¹ (N₂). S₃ registered a significantly lower soil penetration resistance by 22%, 28% and 20%, respectively, at the 17.5, 24.5 and 31.5 cm depths over S₁ and the corresponding decrease over S₂ were 17%, 19% and 13%, respectively. Bulk density after 15 days of tillage operation was significantly low in subsurface (15–30 cm depth) in S₃ (1.39 mg m⁻³) followed by S₂ (1.41 mg m⁻³) and S₁ (1.58 mg m⁻³). Root length density (RLD) and root mass density (RMD) of soybean at 0–15 cm soil depth were greater following subsoiling in every year. S₃ recorded significantly greater RLD (1.04 cm cm⁻³) over S₂ (0.92 cm cm⁻³) and S₁ (0.65 cm cm⁻³) at 15–30 cm depth under this study. The basic infiltration rate was greater after subsoiling in every year (5.65 cm h⁻¹) in relation to conventional tillage (1.84 cm h⁻¹). Similar trend was also observed in water storage characteristics (0–90 cm depth) of the soil profile. The faster infiltration rate and water storage of the profile facilitated higher grain yield and enhanced water use efficiency for soybean under subsoiling than conventional tillage. S₃ registered significantly higher water use efficiency (17 kg ha⁻¹ cm⁻¹) over S₂ (16 kg ha⁻¹ cm⁻¹) and S₁ (14 kg ha⁻¹ cm⁻¹). On an average subsoiling recorded 20% higher grain yield of soybean over conventional tillage but the yield did not vary significantly due to S₃ and S₂. Combined application of 100% NPK and 4 t farmyard manure (FYM) ha⁻¹ in N₂ resulted in a larger RLD, RMD, grain yield and water use efficiency than N₁ or the control (N₀). N₂ registered significantly higher yield of soybean (1517 kg ha⁻¹) over purely inorganic (N₁) (1392 kg ha⁻¹) and control (N₀) (898 kg ha⁻¹). The study indicated that in Vertisols, enhanced productivity of soybean can be achieved by subsoiling in alternate years and integrated with the use of 100% NPK (30 kg N, 26 kg P and 25 kg K) and 4 t FYM ha⁻¹.     

Pruned tea bushes secrete more root exudates to influence microbiological properties in soil

Pruning is adopted at 3–4 years interval as an agronomic practice during tea cultivation. It was hypothesized that biomass loss during pruning will imply stress on tea bushes. The aim of this study was to quantify changes in different parameters (labile organic carbon fractions, phosphatase activity, microbial biomass and microbial respiration) in tea rhizosphere due to pruning by collecting soil samples from the rhizosphere of ten of each pruned and un-pruned tea bushes. Hot-water extractable and dissolved organic C contents in rhizosphere soil of pruned tea were significantly (P ≤ 0.05) higher than those in the soil of un-pruned tea bushes. Analysis of phospholipid fatty acids (PLFA) revealed that the rhizosphere of pruned tea plants had higher population of Gram (+) and Gram (-) bacteria, fungi, actinomycetes and lower denitrifying bacterial population as compared to un-pruned tea plants. Activity of acid phosphatase enzyme in soil was also increased due to pruning. A separate study revealed that de-centering may induce production of up to 50% more labile organic carbon compounds by young tea as compared to un-pruned plants. Therefore, it could be concluded that pruned tea bushes secrete more root exudates to influence microbiological and biochemical properties in rhizosphere.     

Hydrous Ferric Oxide (HFO)—a Scavenger for Fluoride from Contaminated Water

Groundwaters contaminated with excess fluoride (above 1.5 mg/L) posed some risks to the public health in India. Methods available for fluoride contaminated water treatment are cogaulation–filtration using alum + lime + bleaching powder (Nalgonda technique) and adsorption using activated alumina. Use of aluminium compounds for water treatment purpose needs replacement on neurological health problem. Objective to this, the synthesis and fluoride adsorption behavior of hydrous ferric oxide (HFO) are reported here. It is seen that fluoride adsorption density varies as a function of pH, contact time, aging time, drying temperature and particle size of HFO. Highest adsorption density for fluoride is found to be at pH 4.0. Effects of competing anions in removing fluoride from solution were tested. Arsenite, arsenate, phosphate and sulfate show strong interfering effect at high anions to fluoride molar ratio in solution. Adsorption of fluoride on HFO follows the Freundlich isotherm and the Lagergren first-order kinetic model. It was also determined that HFO is a better adsorbent in removing fluoride from high fluoride groundwater than some other adsorbents. Regeneration of fluoride-rich HFO results showed that 1.0 M NaOH solution could be used up to a maximum of 75% regeneration.     

Studies on performance of rice as influenced by drainage in Eastern India

Field experiments were conducted in WTCER research farm, Mendhasal and in the farmer's field at Bishwanathpur, Orissa, India, during two crop years to assess the performance of rice as influenced by drainage at different growth stages. In the first experiment, scented rice variety CR-689-113 was tested with drainage at different growth stages in the main plot and nitrogen levels in the sub plots. In the second experiment, rice variety Swarna was investigated and drainage at different growth stages was provided under the best nitrogen level. The results revealed that drainage at the tillering stage recorded significantly higher grain yield than drainage at all other crop stages. The grain yield of rice was found to be increased by 19–22% when drainage was provided at the tillering stage for an 8–10 day period relative to that of no drainage treatment. However, drainage at the panicle initiation stage recorded the lowest grain yield. Nitrogen at 60 kg ha^?1 resulted in higher grain yield. Crop growth rate and nitrogen use efficiency were found to be higher when drainage was given at the tillering stage.     

Nitrous oxide emissions from kharif and rabi legumes grown on an alluvial soil

Nitrous oxide (N₂O) emissions were monitored for a period of 60 days in a pot culture study, from two kharif (June-September) and two rabi (October-March) season legumes, which were grown on a Typic Ustochrept, alluvial sandy loam soil. Black gram (Vigna mungo L. Hepper), var. T-9, and soybean (Glycine max L. Merril), var. Punjab 1, were taken up in kharif season whereas lentil (Lens esculenta Moench), var. JLS-1, and Bengal gram (Cicer arietinum L.), var. BGD-86, were grown in rabi season. All the crops were grown with and without urea and one pot (containing soil but with no fertilizer or crop) was used as a control. Nitrous oxide emissions were significantly higher in unfertilized cropped soil than in the control, while the addition of urea to the crops further increased the emissions. Significant emissions occurred during third and seventh week after sowing for all the treatments in both kharif and rabi seasons. In kharif, soil cropped with soybean had higher total N₂O-N emission than soil sown with black gram both under fertilized and unfertilized conditions; while in rabi, lentil had a higher total N₂O-N emission than Bengal gram under both fertilized and unfertilized conditions. In kharif, total N₂O-N emissions ranged from 0.53 (control) to 3.84 kg ha^-1 (soybean+urea), while in rabi it ranged from 0.45 (control) to 3.06 kg ha^-1 (lentil+urea). Higher N₂O-N emissions in kharif than in rabi was probably due to the favorable effect of temperature on nitrification and denitrification in the former season. The results of the study indicated that legume crops may lead to an increase in N₂O formation and emission from soils, the extent of which varies from crop to crop.