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991.
R J Matulis F K McKeith D B Faulkner L L Berger P George 《Journal of animal science》1987,65(3):669-674
Cull cows (N = 48) of similar age from Angus and Hereford breed types were assigned to one of four feeding periods (0, 28, 56 or 84 d) and subdivided into two groups, one implanted with Synovex-H and a non-implanted control. Cows were fed a high energy diet and slaughtered at the end of each feeding period. Average daily gain and feed efficiency were evaluated on each animal during the trial. The following carcass traits were evaluated: USDA yield and quality grade characteristics, boning yield, muscle weights, bone weights, pH and Warner-Bratzler shear force of the longissimus muscle. The left side of each carcass was boned and proximate analysis was determined. No significant implant effects were encountered for any of the traits evaluated; therefore, data were pooled and evaluated by feeding period only. Carcass weight and boneless forequarter and hindquarter tissue weights increased significantly between each feeding period; percent kidney, pelvic and heart fat (KPH) and yield grade did not differ (P greater than .05) until d 56 and 84, respectively. Quality grade and marbling scores improved (P less than .05) between 28 and 56 d of feeding. Biceps femoris weight changed during the 84-d experiment from 4.1 kg on d 0 to 5.3 kg on d 84 (P less than .05). Proximate analysis of the boneless tissue indicated an increase (P less than .05) in extractable fat of approximately 6% for each feeding period (6.3% fat on d 0 to 25.4% on d 84).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
992.
Droplets of one liquid suspended in a second, immiscible liquid move through a microfluidic device in which a channel splits into two branches that reconnect downstream. The droplets choose a path based on the number of droplets that occupy each branch. The interaction among droplets in the channels results in complex sequences of path selection. The linearity of the flow through the microchannels, however, ensures that the behavior of the system can be reversed. This reversibility makes it possible to encrypt and decrypt signals coded in the intervals between droplets. The encoding/decoding device is a functional microfluidic system that requires droplets to navigate a network in a precise manner without the use of valves, switches, or other means of external control. 相似文献
993.
Heinrich MC Corless CL Duensing A McGreevey L Chen CJ Joseph N Singer S Griffith DJ Haley A Town A Demetri GD Fletcher CD Fletcher JA 《Science (New York, N.Y.)》2003,299(5607):708-710
Most gastrointestinal stromal tumors (GISTs) have activating mutations in the KIT receptor tyrosine kinase, and most patients with GISTs respond well to Gleevec, which inhibits KIT kinase activity. Here we show that approximately 35% (14 of 40) of GISTs lacking KIT mutations have intragenic activation mutations in the related receptor tyrosine kinase, platelet-derived growth factor receptor alpha (PDGFRA). Tumors expressing KIT or PDGFRA oncoproteins were indistinguishable with respect to activation of downstream signaling intermediates and cytogenetic changes associated with tumor progression. Thus, KIT and PDGFRA mutations appear to be alternative and mutually exclusive oncogenic mechanisms in GISTs. 相似文献
994.
Schotte F Lim M Jackson TA Smirnov AV Soman J Olson JS Phillips GN Wulff M Anfinrud PA 《Science (New York, N.Y.)》2003,300(5627):1944-1947
We report picosecond time-resolved x-ray diffraction from the myoglobin (Mb) mutant in which Leu29 is replaced by Phe (L29Fmutant). The frame-by-frame structural evolution, resolved to 1.8 angstroms, allows one to literally "watch" the protein as it executes its function. Time-resolved mid-infrared spectroscopy of flash-photolyzed L29F MbCO revealed a short-lived CO intermediate whose 140-ps lifetime is shorter than that found in wild-type protein by a factor of 1000. The electron density maps of the protein unveil transient conformational changes far more dramatic than the structural differences between the carboxy and deoxy states and depict the correlated side-chain motion responsible for rapidly sweeping CO away from its primary docking site. 相似文献
995.
Daley GQ Ahrlund Richter L Auerbach JM Benvenisty N Charo RA Chen G Deng HK Goldstein LS Hudson KL Hyun I Junn SC Love J Lee EH McLaren A Mummery CL Nakatsuji N Racowsky C Rooke H Rossant J Schöler HR Solbakk JH Taylor P Trounson AO Weissman IL Wilmut I Yu J Zoloth L 《Science (New York, N.Y.)》2007,315(5812):603-604
996.
Demyelination results in severe disability in many neurodegenerative diseases and nervous system infections, and it is typically mediated by inflammatory responses. Mycobacterium leprae, the causative organism of leprosy, induced rapid demyelination by a contact-dependent mechanism in the absence of immune cells in an in vitro nerve tissue culture model and in Rag1-knockout (Rag1-/-) mice, which lack mature B and T lymphocytes. Myelinated Schwann cells were resistant to M. leprae invasion but undergo demyelination upon bacterial attachment, whereas nonmyelinated Schwann cells harbor intracellular M. leprae in large numbers. During M. leprae-induced demyelination, Schwann cells proliferate significantly both in vitro and in vivo and generate a more nonmyelinated phenotype, thereby securing the intracellular niche for M. leprae. 相似文献
997.
Baumeister W Bachmair A Chau V Cohen R Coffino P Demartino G Deshaies R Dohmen J Emr S Finley D Hampton R Hill C Hochstrasser M Huber R Jackson P Jentsch S Johnson E Kwon YT Pagano M Pickart C Rechsteiner M Scheffner M Sommer T Tansey W Tyers M Vierstra R Weissman A Wilkinson KD Wolf D 《Science (New York, N.Y.)》2004,306(5700):1290-1292
998.
George S Rochford JJ Wolfrum C Gray SL Schinner S Wilson JC Soos MA Murgatroyd PR Williams RM Acerini CL Dunger DB Barford D Umpleby AM Wareham NJ Davies HA Schafer AJ Stoffel M O'Rahilly S Barroso I 《Science (New York, N.Y.)》2004,304(5675):1325-1328
Inherited defects in signaling pathways downstream of the insulin receptor have long been suggested to contribute to human type 2 diabetes mellitus. Here we describe a mutation in the gene encoding the protein kinase AKT2/PKBbeta in a family that shows autosomal dominant inheritance of severe insulin resistance and diabetes mellitus. Expression of the mutant kinase in cultured cells disrupted insulin signaling to metabolic end points and inhibited the function of coexpressed, wild-type AKT. These findings demonstrate the central importance of AKT signaling to insulin sensitivity in humans. 相似文献
999.