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101.
The synergistic activity of tiamulin and chlortetracycline: in-feed treatment of bacterially complicated enzootic pneumonia in fattening pigs 总被引:2,自引:0,他引:2
The antibacterial effects of a combination of tiamulin and chlortetracycline in vitro against a number of field isolates of Pasteurella multocida, Haemophilus pleuropneumoniae and Bordetella bronchiseptica were examined. There was a marked synergism between the two antibiotics against all eight isolates of P multocida, against seven of nine isolates of H pleuropneumoniae and against the single strain of B bronchiseptica tested. Two field trials were carried out on a herd with a history of complicated enzootic pneumonia where the presence of Mycoplasma hyopneumoniae and P multocida had been established and subsequently the presence of H pleuropneumoniae was discovered. Feed containing tiamulin at 100 ppm combined with chlortetracycline at 300 ppm was given for seven days to pigs affected with pneumonia, and the results were compared with untreated controls and pigs receiving chlortetracycline at 300 ppm. There was a follow-up observation period of three weeks when all groups received unmedicated feed. During the medication period the combination treated groups showed a statistically significant increase in average daily weight gain of 156 g (20.4 per cent) and in feed conversion efficiency of 0.576 (20.8 per cent) and a numerical improvement in average disease score in comparison with the untreated controls. These improvements were approximately double those observed in the groups treated with 300 ppm chlortetracycline which showed improvements of 93 g (12.2 per cent) in average daily gain and 0.301 (10.9 per cent) in feed conversion efficiency. During the following three weeks most of the initial gains were lost, probably owing to the reinfection of the treated groups by the untreated controls. 相似文献
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104.
Jones S Prosperi-Porta G Dawe S Blackbourn J Taylor K Lowe G Osborn A 《Folia parasitologica》2004,51(2-3):221-227
An unidentified myxosporean parasite (CKX) is described from the kidney of approximately 80% of spawning coho salmon Oncorhynchus kisutch (Walbaum) in British Columbia, Canada and Washington, United States of America. Morphological features were described using light and electron microscopy. Sequencing of polymerase chain reaction (PCR) amplified 18S ribosomal RNA gene and in situ hybridisation were used to further characterise CKX. The parasite occurred with a focal distribution within tubule epithelial cells, the tubule lumen and the interstitium as primary cells containing from one to at least 16 secondary cells. Luminal stages were degenerate and sporogony was not observed. In situ hybridisation using a digoxygenin-labelled DNA probe confirmed CKX to be the source of DNA used in PCR analyses. CKX 18S rDNA sequences were most similar (97%) to those of Sphaerospora oncorhynchi. Phylogenetic analysis revealed similarities among the 18S rDNA sequences of CKX, S. oncorhynchi and Myxidium lieberkuehni. CKX is hypothesised to be the abortive extrasporogonic development of a Sphaerospora sp. or Myxidium sp. occurring in immune-incompetent spawning and senescent salmon. 相似文献
105.
Rice Pi-ta gene Confers Resistance to the Major Pathotypes of the Rice Blast Fungus in the United States 总被引:1,自引:0,他引:1
Jia Y Wang Z Fjellstrom RG Moldenhauer KA Azam MA Correll J Lee FN Xia Y Rutger JN 《Phytopathology》2004,94(3):296-301
ABSTRACT The Pi-ta gene in rice prevents the infection by Magnaporthe grisea strains containing the AVR-Pita avirulence gene. The presence of Pi-ta in rice cultivars was correlated completely with resistance to two major pathotypes, IB-49 and IC-17, common in the U.S. blast pathogen population. The inheritance of resistance to IC-17 was investigated further using a marker for the resistant Pi-ta allele in an F(2) population of 1,345 progeny from a cross of cv. Katy with experimental line RU9101001 possessing and lacking, respectively, the Pi-ta resistance gene. Resistance to IC-17 was conferred by a single dominant gene and Pi-ta was not detected in susceptible individuals. A second F(2) population of 377 individuals from a reciprocal cross between Katy and RU9101001 was used to verify the conclusion that resistance to IC-17 was conferred by a single dominant gene. In this cross, individuals resistant to IC-17 also were resistant to IB-49. The presence of Pi-ta and resistance to IB-49 also was correlated with additional crosses between 'Kaybonnet' and 'M-204', which also possess and lack Pi-ta, respectively. A pair of primers that specifically amplified a susceptible pi-ta allele was developed to verify the absence of Pi-ta. We suggest that Pi-ta is responsible for resistance to IB-49 and IC-17 and that both races contain AVR-Pita genes. 相似文献
106.
Schuler LA Khaitsa ML Dyer NW Stoltenow CL 《Journal of the American Veterinary Medical Association》2004,225(7):1084-1089
OBJECTIVE: To characterize an outbreak of West Nile virus (WNV) infection in horses in North Dakota in 2002, evaluate vaccine effectiveness, and determine horse characteristics and clinical signs associated with infection. DESIGN: Retrospective study. ANIMALS: 569 horses. PROCEDURE: Data were obtained from veterinary laboratory records, and a questionnaire was mailed to veterinarians of affected horses. RESULTS: Affected horses were defined as horses with typical clinical signs and seroconversion or positive results of virus isolation; affected horses were detected in 52 of the 53 counties and concentrated in the eastern and northeastern regions of the state. Among affected horses, 27% (n = 152) were vaccinated against WNV, 54% (309) were not, and 19% (108) had unknown vaccination status; 61 % (345) recovered, 22% (126) died, and 17% (98) had unknown outcome. The odds of death among nonvaccinated horses were 3 and 16 times the odds among horses that received only 1 or 2 doses of vaccine and horses that were vaccinated according to manufacturer's recommendations, respectively. Horses with recumbency, caudal paresis, and age > 5 years had higher odds of death, whereas horses with incoordination had lower odds of death, compared with affected horses without these characteristics. CONCLUSIONS AND CLINICAL RELEVANCE: Vaccination appears to have beneficial effects regarding infection and death caused by WNV. 相似文献
107.
Sugerman BE Ercolano B Barlow MJ Tielens AG Clayton GC Zijlstra AA Meixner M Speck A Gledhill TM Panagia N Cohen M Gordon KD Meyer M Fabbri J Bowey JE Welch DL Regan MW Kennicutt RC 《Science (New York, N.Y.)》2006,313(5784):196-200
We present late-time optical and mid-infrared observations of the Type II supernova 2003gd in the galaxy NGC 628. Mid-infrared excesses consistent with cooling dust in the ejecta are observed 499 to 678 days after outburst and are accompanied by increasing optical extinction and growing asymmetries in the emission-line profiles. Radiative-transfer models show that up to 0.02 solar masses of dust has formed within the ejecta, beginning as early as 250 days after outburst. These observations show that dust formation in supernova ejecta can be efficient and that massive-star supernovae could have been major dust producers throughout the history of the universe. 相似文献
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109.
Hale G 《The Veterinary record》2006,158(3):108; author reply 108
110.
Pruritus is an important clinical sign and quality-of-life measure in canine dermatology, but can be difficult to assess objectively. Several studies in humans have used activity monitors to measure nocturnal scratching in patients with atopic dermatitis. The results correlate with observation of scratching, scoring in atopic dermatitis indices and levels of inflammatory chemokines. The aim of this study was to examine whether an activity monitor could be used to detect elevated interexercise (i.e. 'resting') activity in atopic dogs compared to healthy dogs. Five healthy dogs and six dogs with atopic dermatitis were fitted with a collar-mounted activity monitor (Actiwatch) that recorded the piezo-electric voltage generated over 15-s epochs for 7 days. Data from defined periods of exercise, playing, etc., were disregarded. Within each group, median (+/- interquartile range) epoch activity was similar during the day (atopic 21.0 [9.8-24.8]; healthy 5.1 [4.6-6.0]) and evening (atopic 19.1 [10.9-25.2]; healthy 5.8 [5.3-11.7]), and significantly lower overnight (atopic 5.8 [4.1-15.7]; healthy 2.5 [1.6-4.4]) (Mann-Whitney test; P < 0.05). The mean epoch activity, however, was significantly higher in atopic dogs compared to healthy dogs for all three time periods (P < 0.05). This study provides preliminary evidence that activity monitors could objectively assess canine pruritus in the normal home environment. 相似文献