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511.
C Canton L Ceballos MP Domínguez C Fiel JP Lirón L Moreno 《New Zealand veterinary journal》2020,68(3):187-192
ABSTRACTAims: The main goal of the current study was to evaluate, on a commercial beef cattle farm, the impact of infection with gastrointestinal nematodes resistant to both ivermectin (IVM) and moxidectin (MXD) on the productivity of calves.Methods: Male Aberdeen Angus calves, aged 9–11 months, with faecal nematode egg counts (FEC) ≥200 epg and body weight ≥190?kg, were allocated to two herds. Herd A (n?=?90) grazed a maize-winter forage crop rotation and Herd B (n?=?90) grazed a 2-year-old Agropyrum pasture. On Day 0 in each herd, calves were randomly allocated into five groups (n?=?18), which were treated with 0.2?mg/kg IVM; 0.2?mg/kg MXD; 3.75?mg/kg ricobendazole (RBZ), both IVM and RBZ, or remained untreated. Faecal samples collected on Days ?1 and 19 were used to determine the percentage reduction in FEC, and genera of the nematodes were determined by the identification of the third-stage larvae recovered from faecal cultures. Total weight gain was determined from body weights recorded on Days ?1 and 91.Results: Overall mean reduction in FEC was 42% for IVM, 67% for MXD, 97% for RBZ and 99% for IVM?+?RBZ. The reduction in FEC for Cooperia spp. was ≤78% for IVM and MXD, and for Haemonchus spp. was 0 and 36% for IVM and MXD, respectively, confirming the presence of parasites resistant to both anthelmintics. Only IVM?+?RBZ treatment resulted in 100% efficacy against Haemonchus spp. The overall estimated mean total weight gain for calves treated with IVM was 15.7 (95% CI?=?11.9–19.7) kg and for calves treated with IVM?+?RBZ was 28.8 (95% CI?=?25–32.5) kg (p?<?0.001). Mean total weight gain for calves treated with MXD was 23.5 (95% CI?=?19.7–27.2) kg.Conclusions and clinical relevance: In calves naturally infected with resistant nematodes, under the production system assessed here, weight gains were lower in calves treated with anthelmintics that were moderately or highly ineffective compared to those treated with highly effective anthelmintics. These results demonstrate to farmers and veterinarians the importance of a sustainable and effective nematode control under field conditions. 相似文献
512.
Francisco Joel Jahuey‐Martínez Gaspar Manuel Parra‐Bracamonte Ana María Sifuentes‐Rincn Victor Ricardo Moreno‐Medina 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2019,136(5):378-389
Charolais cattle are one of the most important breeds for meat production worldwide; in México, its selection is mainly made by live weight traits. One strategy for mapping important genomic regions that might influence productive traits is the identification of signatures of selection. This type of genomic features contains loci with extended linkage disequilibrium (LD) and homozygosity patterns that are commonly associated with sites of quantitative trait locus (QTL). Therefore, the objective of this study was to identify the signatures of selection in Charolais cattle genotyped with the GeneSeek Genomic Profiler Bovine HD panel consisting of 77 K single nucleotide polymorphisms (SNPs). A total 61,311 SNPs and 819 samples were used for the analysis. Identification of signatures of selection was carried out using the integrated haplotype score (iHS) methodology implemented in the rehh R package. The top ten SNPs with the highest piHS values were located on BTA 4, 5, 6 and 14. By identifying markers in LD with top ten SNPs, the candidate regions defined were mapped to 52.8–59.3 Mb on BTA 4; 67.5–69.3 on BTA 5; 39.5–41.0 Mb on BTA 6; and 26.4–29.6 Mb on BTA 14. The comparison of these candidate regions with the bovine QTLdb effectively confirmed the association (p < 0.05) with QTL related to growth traits and other important productive traits. The genomic regions identified in this study indicated selection for growth traits on the Charolais population via the conservation of haplotypes on various chromosomes. These genomic regions and their associated genes could serve as the basis for haplotype association studies and for the identification of causal genes related to growth traits. 相似文献
513.
Cristina Martín‐Moreno Jos Francisco Martín Duque Jos Manuel Nicolau Ibarra Nstor Hernando Rodríguez Miguel ngel Sanz Santos Lzaro Snchez Castillo 《Land Degradation \u0026amp; Development》2016,27(2):145-159
Mining reclamation tries to reduce environmental impacts, including accelerated runoff, erosion and sediment load in the nearby fluvial networks and their ecosystems. This study compares the effects of topography and surface soil cover on erosion on man‐made slopes coming from surface mining reclamation in Central Spain. Two topographic profiles, linear and concave, with two surface soil covers, subsoil and topsoil, were monitored for two hydrologic years. Sediment load, rill development and plant colonization from the four profiles were measured under field conditions. The results show that, in the case of this experiment, a thick and non‐compacted topsoil cover on a linear slope yielded less sediment than carbonate colluvium or topsoil cover on a concave slope. This study also shows that vegetation establishment, which plays an important role in erosion control, depends on topography. Plant cover was more widespread and more homogeneous on linear profiles with topsoil cover. On concave slopes, plant establishment was severely limited on the steepest upper part and favoured in the bottom. This study suggests that management of topography and surface soil cover should be approached systematically, taking three outcomes into consideration: (i) topsoil can lead to a successful mining reclamation regardless of topography, (ii) created concave slopes can lead to a successful mining reclamation and (iii) topography determines the vegetation colonization pattern. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
514.
M. P. Eichhorn P. Paris F. Herzog L. D. Incoll F. Liagre K. Mantzanas M. Mayus G. Moreno V. P. Papanastasis D. J. Pilbeam A. Pisanelli C. Dupraz 《Agroforestry Systems》2006,67(1):29-50
Mixed systems of agriculture incorporating combinations of trees and crops have formed key elements of the landscape of Europe
throughout historical times, and many such systems continue to function in the present day. In many cases they represent formerly
widespread traditional systems in decline and a number have already become extinct or exist only in a threatened state. The
causes are both practical and economic. The agricultural subsidy regime within the European Union is presently unfavourable
towards silvoarable practices, which has been a major factor in their recent decline. The silvoarable systems of Europe can
be split into two classes according to location – northern Europe and the Mediterranean. The latter contains not only a greater
area of silvoarable cultivation, but also a greater diversity of systems due to the broader range of commercial tree and crop
species grown. In general, the systems of northern Europe are limited by light, whilst those of the Mediterranean are limited
by the availability of water. Mixed systems of agriculture present an opportunity for future European rural development and
have the potential to contribute towards the increased sustainability of agriculture and enhancement of biodiversity, whilst
preserving landscapes that are both culturally important and aesthetically pleasing. A better understanding of the legacy
of traditional silvoarable systems, combined with the formulation of a consistent definition and specific European policy
towards them will be invaluable in ensuring that the benefits of mixed agriculture are fully exploited in the future. 相似文献
515.
Moreno MJ Abad A Pelegrí R Marínez MJ Sáez A Gamón M Montoya A 《Journal of agricultural and food chemistry》2001,49(4):1713-1719
The N-methylcarbamate pesticide carbofuran is a very important insecticide used worldwide. In the present work, the validation of a monoclonal antibody-based enzyme immunoassay (ELISA) to determine this compound in fruits and vegetables is described. The immunoassay is a competitive heterologous ELISA in the antibody-coated format, with an I(50) value for standards in buffer of 740 ng/L and with a dynamic range between 200 and 3100 ng/L. For recovery studies, peppers, cucumbers, strawberries, tomatoes, potatoes, oranges, and apples were spiked with carbofuran at 10, 50, and 200 ppb. After liquid extraction, analyses were performed by ELISA on extracts purified on solid-phase extraction (SPE) columns and crude, nonpurified extracts. Depending on the crop, mean recoveries in the 43.9--90.7% range were obtained for purified samples and in the 90.1--121.6% range for crude extracts. The carbofuran immunoassay performance was further validated with respect to high-performance liquid chromatography (HPLC) with postcolumn derivatization and fluorescence detection (EPA Method 531.1). Samples were spiked with carbofuran at several concentrations and analyzed as blind samples by ELISA and HPLC after SPE cleanup. The correlation between methods was very good (y = 0.90x + 2.66, r(2)() = 0.958, n = 25), with HPLC being more precise than ELISA (mean coefficients of variation of 4.1 and 11.5%, respectively). The immunoassay was then applied to the analysis of nonpurified extracts of the same samples. Results also compared very well with those obtained by HPLC on purified samples (y = 1.02x + 10.44, r(2)() = 0.933, n = 29). Therefore, the developed immunoassay is a suitable method for the quantitative and reliable determination of carbofuran in fruits and vegetables even without sample cleanup, which saves time and money and considerably increases the sample throughput. 相似文献
516.
Mangas JJ Moreno J Rodríguez R Picinelli A Suárez B 《Journal of agricultural and food chemistry》1999,47(1):152-156
A feasible spectrophotometric method for determining acidic and neutral polysaccharides in cider is described, and the advantages of this method are examined with respect to precision, accuracy, and detection and quantitation limits. The concentration of nonvolatile and volatile components in cider, together with chemometric techniques such as principal component analysis (PCA), soft independent modeling of class analogy (SIMCA), and partial least squares (PLS), allowed us to typify the ciders on the basis of their foaming properties. Acidic polysaccharide and 1-propanol were the most relevant variables for this purpose. 相似文献
517.
Macario?Bacilio Hilda?Rodriguez Manuel?Moreno Juan-Pablo?Hernandez Yoav?BashanEmail author 《Biology and Fertility of Soils》2004,40(3):188-193
Root colonization and mitigation of NaCl stress on wheat seedlings were studied by inoculating seeds with Azospirillum lipoferum JA4ngfp15 tagged with the green fluorescent protein gene (gfp). Colonization of wheat roots under 80 and 160 mM NaCl stress was similar to root colonization with this bacterial species under non-saline conditions, that is, single cells and small aggregates were mainly located in the root hair zone. These salt concentrations had significant inhibitory effects on development of seedlings, but not on growth in culture of gfp-A. lipoferum JA4ngfp15. Reduced plant growth (height and dry weight of leaves and roots) under continuous irrigation with 160 mM NaCl was ameliorated by bacterial inoculation with gfp-A. lipoferum JA4ngfp15. Inoculation of plants subjected to continuous irrigation with 80 mM NaCl or to a single application of either NaCl concentration (80 or 160 mM NaCl) did not mitigate salt stress. This study indicates that, under high NaCl concentration, inoculation with modified A. lipoferum
reduced the deleterious effects of NaCl; colonization patterns on roots were unaffected and the genetic marker did not induce undesirable effects on the interaction between the bacterium and the plants. 相似文献
518.
Moreno José L. Pérez Aurelia Aliaga Asunción Hernández Teresa 《Water, air, and soil pollution》2003,143(1-4):289-300
Nickel pollution may affect microbial communitydevelopment and its activity in soil and, therefore, itsfertility, while organic amendment may affect Ni mobilityand bioavailability. The aim of this research was toascertain the extent to which the addition of sewage sludgeto soil may affect Ni toxicity and to establish theecological dose of this heavy metal. Unamended and amendedsoils with sewage sludge were spiked with differentconcentrations of Ni (0–8000 mg kg-1) and then incubatedfor 3 hours, 12 days and 40 days. Soil dehydrogenaseactivity, ATP content, microbial respiration and microbialbiomass C were measured in the samples containing differentNi concentrations. A mathematical model which describe theinhibition of these soil parameters were used to calculatethe ecological dose of Ni: ED50. Soil microbialbiomass C measurements of all treatments better fitted themodel (r2 = 0.95–0.69 with p < 0.05) than the otherparameters studied; but the ED50 values predicted by the modelhad a high coefficient of variation. For both ATP contentand microbial biomass C, the ED50 values calculatedfor the amended soil were higher than for the unamendedsoil and so that the effect of Ni toxicity on theseparameters of microbial activity may be considered lower inthe former. In this study, an increase in ED50 valueswith the time elapsed was also observed. 相似文献
519.
M. V. Martinez-Toledo J. Gonzalez-Lopez T. de la Rubia J. Moreno A. Ramos-Cormenzana 《Biology and Fertility of Soils》1988,6(4):352-353
Summary Field experiments were conducted during 1985 and 1986 to examine the effect of Azotobacter chroococcum on the grain yield of maize. Application of 40 kg N ha–1 plus A. chroococcum caused a significant increase in maize yield. Azotobacter inoculation was more efficient at lower doses (40 kg N ha–1) than at high doses (80 kg N ha–1) of urea. 相似文献
520.
Muñoz D Peinado RA Medina M Moreno J 《Journal of agricultural and food chemistry》2005,53(13):5258-5264
Sherry wines obtained after biological aging for an average of 0, 2, and 4 years were inoculated separately with the flor yeast strains Saccharomyces cerevisiae var. capensis and Saccharomyces bayanus and subjected to short, periodic microaeration to a dissolved oxygen concentration of 4 mg L(-1) after formation of the yeast film. A principal component analysis with the acetaldehyde, ethanol, volatile acidity, and glycerol concentrations obtained was performed. The first principal component was found to account for 49.5% of the overall variance and to be defined mainly by glycerol and ethanol. The second component accounted for 38.8% of the variance and was defined by volatile acidity and acetaldehyde. The conditions used in the tests allowed the biological aging of the wines to be substantially shortened. Thus, 42 days after flor-film formation by S. cerevisiae var. capensis, 0- and 2-year-old wines exhibited parameter values similar to those obtained for the wine aged for 4 years. The wines inoculated with S. bayanus exhibited high acetaldehyde concentrations and ethanol levels above 15% (v/v)-sherry wines with alcohol concentrations below 14.5% are undesirable-, so one need not exclude the sequential or simultaneous inoculation of S. bayanus together with S. cerevisiae var. capensis in order to improve the biological aging process. 相似文献