“西育1号”芹菜周年栽培技术

"西育1号"芹菜新品种于2009年10月通过陕西省农作物品种审定委员会登记。经过在西安三桥农场、西安市现代农业科技展示中心多年的多茬口栽培试验以及高陵、阎良、周至等区县的试验示范,总结形成了一套适合关中地区"西育1号"芹菜的优质高效周年栽培技术,以期为该品种的推广提供技术指导。     

Real-time Vehicle Scheduling

The traditional scheduling for vehicle is option in the fixed network. But it can't satisfy the real time environment. The demand of order is changeable and uncertain in real environment. It is convenient for scheduling transforming it into static data, but can't get the advantage on time. This paper proposes a real time vehicle scheduling on the study of traditional scheduling and discusses the model structure and the key technology for the real time system that establishes the base for develop teal time system.     

旱田中耕节水灌溉设备的研究

分析了节水灌溉现状；以玉米苗期为研究对象，结合黑龙江省旱作农业实际，提出了一种与中耕相结合的灌溉方式，为采用机械化灌溉方法解决作物苗期干旱问题提供参考。     

饲用复合酶对蛋用种鸡日粮磷的利用和生产性能的影响

本试验旨在研究低磷日粮添加复合酶制剂对蛋用种鸡磷的利用和生产性能的影响。选用972只24周龄罗曼褐父母代种鸡,分为3个处理,每处理6个重复,每重复54只种鸡。采用玉米-豆粕-杂粕型基础饲粮,正对照组含总磷0.55%,非植酸磷0.40%,不加酶;负对照组含总磷0.40%,非植酸磷0.21%,不加酶;试验组含总磷0.40%,非植酸磷0.21%,加酶制剂。试验期18周。结果表明:在低非植酸磷的日粮中添加复合酶,粪便中磷含量极显著低于正对照组(P<0.01);粪便中粗蛋白质含量显著低于正对照组和负对照组(P<0.05);显著改善蛋黃顔色(P<0.05);不影响生产性能、骨骼发育和蛋壳质量。添加200 g/t复合酶替代罗曼褐蛋用种鸡日粮中75%的磷酸氢钙能够降低饲料成本,提高养殖企业的经济效益,对罗曼褐蛋用种鸡生产性能无不利影响。     

地理信息系统在森林分类经营中的应用

以黑龙江省大兴安岭林管局新林林业局的森林资源为研究对象,以森林资源的分类经营为研究目标,通过对各种二类调查因子(如海拔、坡度、地类、地被物等)的分析,提出森林分类经营的区划依据,并通过地理信息系统软件ARCVIEW 3.0实现了这一区划,最后提出塔源林场的森林分类经营区划图。     

艾纳香脱氢酶基因家族的生物信息学分析

采用RT-PCR和RACE（Rapid amplification of cDNA ends）技术,从艾纳香（Blumea balsamifera (L.) DC.）叶片中克隆到4条编码艾纳香脱氢酶（BbADH1、BbADH2、BbADH3、BbADH4）基因的cDNA序列,并对4条核苷酸及其编码的氨基酸序列进行生物信息学分析。结果显示:4条艾纳香脱氢酶序列开放阅读框均在900 bp左右,蛋白质等电点（pI）值在5.0~9.0之间,含量最多的氨基酸为亮氨酸（Leu）,最少的为色氨酸（Try）,具有明显的疏水区和亲水区,N端未发现信号肽,且无跨膜区;同源性比对结果显示,艾纳香BbADH蛋白与其他植物中ADH蛋白具有高度的相似性,且具有脱氢酶的特征功能域;系统发育分析表明,艾纳香（B. balsamifera (L.) DC.）BbADH1和BbADH3同处于一个分支,且与胡杨（Populus euphratica Oliv.）PeADH物种亲缘关系较近,而艾纳香BbADH4和BbADH2处于不同分支,且分别与葡萄（Vitis vinifera）VvADH和烟草（Nicotiana tabacum）NtADH物种亲缘关系较近。     

辣木的染色体制片优化及核型分析

以辣木分生组织为材料,采用酶解去壁低渗法制片,探讨不同取材部位、预处理方式和酶解时间对辣木染色体制片的影响,并对其进行核型分析,以期为辣木的起源、演化及遗传育种提供一定理论依据。结果表明:以辣木新枝茎尖为最佳取材部位,用饱和对二氯苯预处理2 h,再用4%纤维素酶和5%果胶酶混合物酶解4 h,制片所得染色体效果最佳。核型分析表明,辣木染色体属于小染色体,有28条,核型公式为2n=2x=2n=28m,属于1B类型,核型不对称系数60.29%,核型对称程度较高,这表明辣木在进化中可能处于比较原始类型。     

Mechanism of elemene enhancing radiosensitivity of human glioma U251 cells

AIM To investigate the effect of elemene on the radiosensitivity of human glioma U251 cells and its mechanism. METHODS The U251 cells were used as a glioma model in vitro, and were exposed to different concentrations of elemene and different doses of radiation. The cell viability was measured by MTT assay, the apoptosis and cell cycle distribution were analyzed by flow cytometry, and the related protein levels were determined by Western blot. RESULTS Elemene inhibited the viability of U251 cells in vitro and enhanced the radiosensitivity of the cells. The cells in radiotherapy combined with elemene group had higher rates of early apoptosis, secondary necrosis and total cell death than those in radiation group. Elemene induced G₂/M phase arrest in the U251 cells. Elemene reduced the protein expression of cell division cycle protein 2 （Cdc2）, which resulted in the decrease in cyclin B1 expression induced by radiotherapy, thereby inhibiting the formation of cyclin B-Cdc2 complex. Elemene reduced Cdc2 activity by inhibiting the phosphorylation of Cdc2 protein at threonine 161, thereby inducing G₂/M phase arrest in the cells. It also mediated apoptosis by down-regulating survivin expression. CONCLUSION Elemene may increase the sensitivity of U251 cells to radiotherapy by down-regulating Cdc2 protein, decreasing cyclin B1 expression, inhibiting the formation of cylcin B-Cdc2 complex and down-regulating the expression of survivin.