多媒体技术在《动物微生物》教学中的应用

多媒体技术在现代教学中应用广泛,成效也日益显著,在《动物微生物》教学中应用多媒体技术进行教学,既可丰富学生的理论知识,又能促进学生动手能力的提高。     

基于遥感和GIS的黄河三角洲淤蚀动态研究

采用遥感和地理信息系统 (GIS)技术 ,研究黄河三角洲地区 1976 - 0 6～ 1998- 0 5淤积与侵蚀面积的动态变化 ,通过对不同时相遥感图像的解译提取其水陆界线 ,在 GIS支持下对黄河三角洲地区的水陆边界进行比较 ,确定侵蚀区与淤积区 ,并计算其面积。结果发现 ,黄河三角洲地区 1976 - 0 6～ 1981- 0 9年净增面积最大 ,平均每年增加 2 90 8.2 75 hm2 ,1989- 11～ 1992 - 0 9年净增面积最少 ,仅为 5 13.5 98hm2。总的来看 ,黄河三角洲的淤积速度呈现为递减的趋势 ,主要的淤积区为黄河入海口处 ,侵蚀区为河口北部和东部沿海地区。     

PCV-2感染对PK-15细胞抗病毒相关因子转录时相的影响

【目的】了解猪圆环病毒2型(PCV-2)感染对PK-15细胞抗病毒相关因子转录时相的影响,探讨宿主-病毒之间的作用关系。【方法】运用荧光定量PCR技术,测定和分析PCV-2感染PK-15细胞后0(未接种病毒),1,6,12,24,48,72 h时病毒DNA含量及细胞因子IFN-βI、FN-γI、FNAR-1I、FNAR-2、MHC-Ⅰ、MHC-Ⅱ、MX1、NOS、RNaseL和IRF-3 mRNA转录水平的变化。【结果】PCV-2感染PK-15后1 h就可以检测到病毒DNA,在感染后24 h病毒开始大量迅速增殖,于感染72 h时达到最高峰。与0 h相比,PCV-2感染后IFN-βI、RF-3的表达量在感染12 h时显著增加,其他时间表达量下降;IFN-γ、MHC-Ⅱ的表达量在感染12 h时增加不明显,其他时间表达量下降;感染PCV-2后IFNAR-2、MHC-Ⅰ、MX1的表达量下降;RNaseL的表达量在感染12 h时显著减少,其他时间表达量增加,48 h时表达量增加显著;IFNAR-1的表达量在感染72 h时显著增加,其他时间表达量下降;未检测出NOS的表达。【结论】随着PCV-2病毒含量的增加,以上几种主要抗病毒因子的表达量不明显或有所下降,表明PCV-2感染PK-15细胞后可逃避机体的抗病毒作用机制。     

正交试验法筛选人参皂苷提取工艺的研究

目的筛选人参皂苷最佳提取工艺。方法采用正交试验法进行优选,高效液相色谱法测定人参皂苷Rg1和人参皂苷Re的含量。结果提取次数和乙醇浓度对提取工艺有显著影响。结论人参皂苷最佳提取工艺为A2B2C2D3,即药材用70%乙醇回流提取3次,每次加醇8倍量,提取时间1.5h。     

Technology and Infrastructure Considerations for E-Commerce in Chinese Agriculture

Information technology （IT） was one of the most intensively studied and extensively applied technologies in the 20th century and its research and application will be even more accelerated in the 21st century. The impact of IT is more farreaching than any one may have had imagined, encompassing all facets and sectors of society. Economically, e-commerce generated more than $1.5 trillion in the US in 2004 and is projected to be 30, 15 and 2.6% of the total manufacturing, whole sales and retail sales in the US in 2006 respectively. IT research in agriculture has so far been particularly applied to such fields as precision farming and bioinformatics and in extension and farming practices. As such, its potential application in agricultural e-commerce has only begun to be explored. The United States Department of Agriculture has identified four different functions that e-commerce firms perform in the agricultural sector. They are： （1） information distribution, （2） input supply, （3） commodity trading floor, and （4） logistics/supply chain management. Unlike other industrial products, agricultural products are much more diverse and difficult to process and handle. For instance, fruits and vegetables are perishable and have to reach the market and the hands of consumers within a short period of time. One of the problems for IT applications in agriculture is the lack of standard measures in characteristics and quality of perishable produce and processing foods. Standards are pre-requisites for effective IT application and e-commerce. According to the Global Commerce Initiative, standards bring scalability, portability and affordability to the business process. Standards speed up the supply chain and reduce errors and protect technology investments and ensure system to system interaction and interoperability. Because the complexity of issues involved in standardization, the process of standardization must involve the input and discussion from all segments of the society. Being the largest agricultural country in the world and one of the most active trading partners in the international market, China must also be involved in determining the standards for both domestic and international trades. These measures are the necessary foundations for e-commerce in agriculture. In this paper, we provide a review of the technological development of standardization and coding that are prerequisites for IT application in e-commerce. This understanding is important for professionals in China to promote e- commerce in agriculture. Finally, we propose that a national committee to be formed in China, which includes IT experts from private sectors, academic professionals and governmental officials. This committee should consider the progress being made elsewhere in the world, the unique properties of Chinese commodities and appropriate business models to determine a set of standard measures that are transportable and communicable digitally in the world market for China＇s e- commerce in agriculture.     

关于农产品物流的供求状况分析及对策

无农不稳，无商不活，要提高我国农业发展活力，大力发展正在兴起的农产品物流是一条重要途径。从农产品物流的需求与供给状况入手分析，当前存在的问题及影响其发展的主要原因，即为流通渠道结构单一、成本高、技术落后等，今后应从增强农业各部门对农产品物流的认识，改进技术，加强基础设施建设，发展第三方物流等方面着手并加以重视，加大对农产品物流的发展。     

太行山羊遗传检测

应用随机整群抽样方法，对太行山羊的两个群体共72只成年羊，用淀粉凝胶电泳法测定了33个血液蛋白座位，并对毛色和外形特征进行了检测。结果表明，运铁蛋白（Tf）、前白蛋白－3（Pa－3）、肽酶－B（Pep-B）、碱性磷酸酶（Alp）、亮氨酸胺肽酶（LAP）和酯酶－D（Es-D)6个血液蛋白座位表现多态性，多态位点比例为0．1818；12个类别 25种外型特征中7个有变异体；4个毛色座位有变异。平均杂合度分析表明，太行山羊变异程度较大（H＝0．555），说明太行山羊群体有效规模较大，目前尚未面临急 待保种的局面.     

马属动物急性失血时的心电图变化

对马属动物静脉放血,在此过程中动态描记心电图,并同时监测动脉血压和中心静脉压等指标,以了解并分析急性失血时动物心功能和血液动力学的变化。     

近视眼患者头发中钙、镁、锌、铜含量检测分析

用原子吸收法测定了45例近视眼患者及34例正视眼者头发中Ca、Mg、Zn、Cu元素的含量。结果表明:近视眼患者头发中Zn、Cu、Ca含量显著低于正视眼(P<0.05),Mg含量与正视眼者差异不显著。揭示人体内Zn、Cu、Ca元素含量与近视眼发病存在一定关系。     

N-苯基-N′-单氯代苯基脲的合成及生物活性研究

在三乙胺或三正丁胺存在下,一氯苯胺与氯甲酸甲酯加热回流生成一氯苯胺基甲酸甲酯对氯代苯基,后者不经分离再与过量苯胺反应生成N-苯基-N′-邻氯代苯基脲、N-苯基-N′-间氯代苯基脲和N-苯基-N′-对氯代苯基脲3种化合物,其中以N-苯基-N′-间氯代苯基脲的产率最高。采用红外光谱和质谱对目标化合物进行了结构表征。以丰光萝卜种子为材料,采用萝卜子叶扩张生长法测定了3种化合物促进细胞分裂的活性,结果表明,合成的3种化合物质量浓度为1.00～0.01mg/L时,都能显著促进萝卜子叶生长,其中以1.00mg/L的N-苯基-N′-间氯代苯基脲的活性最高。