Diagnosis of naturally occurring Fasciola hepatica infections in cattle with an enzyme-linked immunosorbent assay

Sera from 100 herds of cattle located in the state of Washington were examined with an enzyme-linked immunosorbent assay for antibody to Fasciola hepatica in a screening procedure that included 5 to 10 samples/herd. Twenty-eight herds contained infected cattle and F hepatica was most prevalent in 3 distinct geographic areas. Subsequent retesting of all sera available from 14 herds (mean of 109 samples/herd) revealed that the screening procedure correctly detected 7 of 7 operations in which greater than 40% of samples were positive or suspect and 3 of 3 operations in which 12% to 13% of the samples were positive or suspect. One of 3 herds considered negative after screening was found to contain a few (7%) positive samples and 1 herd considered possibly infected was negative on retest. These results were compared with those obtained by fecal examination for F hepatica eggs in 9 of the 14 herds. A good correlation (5 of 5) was found in which a high percentage (48% to 85%) of sera were positive or suspect. Fasciola eggs were not found in samples from 2 herds with few (7% to 12%) positive or suspect sera or in 2 herds that were negative by an enzyme-linked immunosorbent assay.     

刚棘颚口线虫的扫描电镜观察

刚棘颚口线虫扫描电镜显示：头球顶端有两片半球形的大侧唇，大侧唇背面有1对双乳突和1个头感器。在轮节皮棘第6-7排或第8-9排或第10-11之间各有1对颈乳突，与樊氏等及古贺正崇等描述颈乳突位置有所差异。并观察到雄虫尾端长交合刺的皮纹上有细小的凹陷小乳，其顶端有开口处；有2对尾乳突。雌虫阴门位于虫体腹面中线之后；尾端前腹面有半月状的肛门，有1对尾乳突。其中头钩、体棘、交合刺和尾乳突等形态特征与前人记述相似。     

蒽酮-硫酸比色法检测多糖条件的优化

本研究通过单因素实验和L9(33)正交实验优化蒽酮-硫酸比色法检测多糖含量的条件,研究反应温度、反应时间、蒽酮-硫酸液的体积对吸光度值的影响。结果表明,蒽酮-硫酸比色法检测的糖含量的最优条件为蒽酮-硫酸2 mL,于100℃反应10 min。验证实验结果表明,总标糖获得的标准曲线方程A=0.005W-0.0162,r=0.9998,优于葡萄糖标准曲线方程。在620 nm下测定,能得到较高的吸光度值,重现性好。     

凋亡素基因真核表达载体的构建及在人肿瘤细胞中的表达

为探讨凋亡素对肿瘤的基因治疗效果,构建了凋亡素基因的真核表达载体。将凋亡素基因重组入 ｐｃ Ｄ Ｎ Ａ３ 载体,并通过脂质体介导凋亡素在人喉癌、人肺癌细胞系中表达;通过 Ｒ Ｔ Ｐ Ｃ Ｒ 在转染细胞中检测到了凋亡素的 ｍ Ｒ Ｎ Ａ,这为应用凋亡素进行肿瘤的基因治疗奠定了基础。     

桑尺蠖和丝棉木金星尺蠖的微孢子虫对家蚕病原性和胚种传染性研究

从桑尺蠖（ＨｅｍｅｒｏｐｈｉｌａａｔｒｉｌｉｎｅａｔａＢｕｔｌｅｒ）中分离的微孢子虫（简称桑尺蠖微孢子虫），形态为长卵圆形，大小为３．５～４．１×１．６～１．９μｍ。从丝棉木金星尺蠖（ＣａｌｏｓｐｉｌｏｓｓｕｓｐｅｃｔａＷａｒｒｅｎ）中分离的微孢子虫（简称丝棉木金星尺蠖微孢子虫），形状为卵圆形，大小为３．２～３．７×１．６～２．１μｍ。两种微孢子虫均能感染寄生家蚕的主要组织器官，引起蚕儿发病，但致病力要比家蚕微孢子虫（Ｎｏｓｅｍａｂｏｍｂｙｃｉｓ）弱，且能经卵传染给下一代，其胚种传染率要比家蚕微孢子虫低。     

嘉兴平原桑园土壤分类及肥力的调查

选择浙北嘉兴平原桑园代表性土壤，进行调查，研究其成因、分类及特性，并对高低产桑园土壤的理化性状进行化验分析，明确了嘉兴平原桑园土壤是历史上由人工堆迭而成，在分类系统上归属于潮湿土纲、正常潮湿土亚纲、潮土土类、暗潮土亚类、堆迭土土属，又分为粘质堆迭土、壤质堆迭土、粉质堆迭土３个土种。在统计众多土壤理化性状数据的基础上，暂拟本区高产桑园土壤１６项理化性状，作为本地区桑园地力建设的参考。     

丝素/明胶共混膜的结构与溶解性能的初探

将丝素溶液与明胶溶液按不同共混比在不同条件下进行混合,制备成丝素/明胶共混膜。用环境扫描电镜、红外光谱仪、差示扫描量热分析系统分别测定共混膜的结构与热性能,采用烘干质量法测定共混膜的溶胀率和溶解率。结果表明:丝素与明胶的共混性好,共混膜结构改变,热稳定性好;烘干时间对共混膜的溶胀率和溶解率影响不显著,不同浓度乙醇处理对溶胀率有极显著影响,不同共混比则对溶胀率和溶解率都有极显著影响。     

肉鸭实验性锰缺乏症及高磷对缺锰影响的病理学研究

６９只一日龄天府肉鸭随机分为三组，各喂以基础、高磷、对照。试验分为两个阶段，第一阶（０－４周）结束时，每组各留２只鸭继续进行第二阶段（５－２０或２６周）试验。缺锰组第一周开始发病，第二周迅速达到高峰。     

克伦特罗缓释药囊的制备,体外释药及对育肥猪生产性能的影响

选用可生物降的白氨酸，谷氨酸甲酯，谷氨酸三元聚物制成克伦特罗缓释药囊。体外释药研究所表明，药囊能保持长期缓慢恒速释药，释药速率随取合物中谷氨酸含量增多而增大，其中ＰＬＭＧＧ－２５的克伦特罗药囊释药时间为８０ｄ、稳态后每天释药量平均为１．２３ｍｇ／ｄ。