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81.
PCR在安祖花和白鹤芋属间杂交种早期鉴定中的应用 总被引:3,自引:0,他引:3
采用以PCR技术为基础的tRNALeuCAA基因内序列细胞核标记和内部改写空间2(ITS2)序列2种方法对7个安祖花属的红鹤芋(Anthurium andraeanum)和白鹤芋属的矮小苞叶芋(Spathiphyllum wallissii)的属间杂交种进行鉴定.使用tRNALeuCAA基因内序列细胞标记共扩增出13个位点,供试杂种中均无父本特异性谱带出现,仅在1个杂种中出现新的谱带,表明该杂种为真正的属间杂种.ITS2序列法扩增出28个位点,杂种谱带表现3种类型:无父本特异性谱带出现;出现新谱带;既出现父本特异性谱带又出现新谱带.应用ITS2序列法有6个假定杂交种均被鉴定为真正的属间杂交种.试验结果表明,tRNALeuCAA基因内序列细胞核标记和内部改写空间2(ITS2)序列2种方法可以用于属间杂种的鉴定. 相似文献
82.
S C To 《Modern veterinary practice》1984,65(1):39-41
Of 12 pregnant swine (vaccinates) given a 4-pilus enterotoxigenic E coli (ETEC) bacterin (K88, K99, 987P, F41), all developed comparable or significantly higher serum and colostral antibody levels than those of 8 pregnant swine (controls) given a 3-pilus ETEC bacterin (K88, K99, 987P). When piglets were challenged with an ETEC strain bearing the F41 antigen, those from vaccinates had significantly lower mortality, less scours, less severe clinical signs and better weight gain than those from controls. 相似文献
83.
84.
Elzbieta Gujska Joanna Michalak Joanna Klepacka 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(2):129-134
High-performance liquid chromatography was used to study the stability of folate vitamers in two types of rye breads after
baking and 16 weeks of frozen storage. Bread made using sourdough seeds contained less total folate (74.6 μg/100 g dry basis,
expressed as folic acid) than the whole rye flour (79.8 μg/100 g dry basis) and bread leavened only with baker’s yeast (82.8 μg/100 g
dry basis). Most importantly, it was generated by a significant decrease in 5-CH3-H4folate form. The baking process caused some changes in folate distribution. Storage of breads at -18°C for 2 weeks did not
have a significant effect (p < 0.05) on total folates compared to the content directly after baking. After a 5-weeks storage
period, a significant decrease (p < 0.05) in the content of total folates was recorded and it dropped on average by 14% for
both type of breads. After a longer period of storage (16 weeks), a 25% loss of folates in the bread made with baker’s yeast
and a 38% loss in the bread fermented with sourdough seeds was found. Retention of 5-CH3-H4folate and 10-HCO-H2folate forms were much lower in the bread made with a sourdough addition than with baker’s yeast only. 相似文献
85.
介绍了波兰的樱桃产量、面积和分布,主要品种和栽培模式,为中国的樱桃产业发展提供了系统的产业信息。 相似文献
86.
Slawomir Rump Teresa Gidynska Elzbieta Galecka Oktawiusz Antkowiak Marek Kowalczyk 《Pest management science》1999,55(10):1033-1035
The summary deals with the anti-convulsant and antilethal effects of a new benzodiazepine receptor partial agonist, imidazenil, in DFP intoxication. It has been demonstrated that imidazenil (2–5 mg kg−1) significantly decreases convulsion intensity, rapidly inhibits seizure patterns in brain bioelectrical activity and significantly increases the anti-lethal efficacy of atropine plus obidoxime therapy. These effects are comparable to diazepam at 5 mg kg−1. However, diazepam exhibits myorelaxant activity at therapeutic doses, which are only observed at 5–10 times the therapeutic doses of imidazenil. 相似文献
87.
Formulations containing citrus polymethoxylated flavones (PMFs), mainly tangeretin, or citrus flavanone glucosides, hesperidin and naringin, were evaluated for cholesterol-lowering potential in hamsters with diet-induced hypercholesterolemia. PMF metabolites were also investigated. Diets containing 1% PMFs significantly reduced serum total and very low-density lipoprotein (VLDL) + LDL cholesterol (by 19-27 and 32-40%, respectively) and either reduced or tended to reduce serum triacylglycerols. Comparable reductions were achieved by feeding a 3% mixture of hesperidin and naringin (1:1, w/w), implying lower hypolipidemic potency of the hesperidin/naringin vs PMFs. HPLC-MS analysis identified high serum, liver, and urine concentrations of tangeretin metabolites including dihydroxytrimethoxyflavone and monohydroxytetramethoxyflavone glucuronides and aglycones. Total liver concentrations of tangeretin derivatives corresponded to hypolipidemic concentrations of intact tangeretin in earlier experiments in vitro. This suggests that PMFs are novel flavonoids with cholesterol- and triacylglycerol-lowering potential and that elevated levels of PMF metabolites in the liver might be directly responsible for their hypolipidemic effects in vivo. 相似文献
88.
Nougayrède JP Homburg S Taieb F Boury M Brzuszkiewicz E Gottschalk G Buchrieser C Hacker J Dobrindt U Oswald E 《Science (New York, N.Y.)》2006,313(5788):848-851
Transient infection of eukaryotic cells with commensal and extraintestinal pathogenic Escherichia coli of phylogenetic group B2 blocks mitosis and induces megalocytosis. This trait is linked to a widely spread genomic island that encodes giant modular nonribosomal peptide and polyketide synthases. Contact with E. coli expressing this gene cluster causes DNA double-strand breaks and activation of the DNA damage checkpoint pathway, leading to cell cycle arrest and eventually to cell death. Discovery of hybrid peptide-polyketide genotoxins in E. coli will change our view on pathogenesis and commensalism and open new biotechnological applications. 相似文献
89.
90.
Laura Vanderkam 《中国禽业导刊》2013,(19):41-41
一批评者:学会管理要靠实践.而不能靠课程有批评者认为:学会管理要靠实践,而不能靠课堂学习。MBA课程培养出来的是脱离实战的“皇太子”,虽然理论高深,但他们对将要经营的公司却一无所知。社会上真正需要的是靠实践成长起来的企业家,而不是靠书本理论培养出来的专业经理人。那么,MBA专业的目的到底是什么? 相似文献