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排序方式: 共有912条查询结果,搜索用时 31 毫秒
191.
Alexander N. Shikov Ekaterina D. Obluchinskaya Elena V. Flisyuk Inna I. Terninko Yulia E. Generalova Olga N. Pozharitskaya 《Marine drugs》2022,20(5)
In recent years, natural deep eutectic solvents (NADES) have been widely investigated for the extraction of food and medicinal plants as well as seaweeds. However, the ability of NADES for trace elements co-extraction from natural sources is not well investigated. The aim of this study was to investigate the ability of common NADES for trace elements co-extraction from Fucus vesiculosus. All of the tested NADES did not recover As and Co (concentration <LOQ). Moreover, all of the tested NADES provided a low recovery (<9%) of Ba, Ca, Fe, Mg, Mn, Sr, and Zn. The method of extraction had not shown a statistically significant effect on the co-extraction of all elements (excluding Ba and Ca). In contrast, the water content in NADES was significantly affected on the recovery of Ba, Ca, Mg, Mn, Sr, and Zn. The recovery of Al and Cr was relatively high and considerably varied (from 1.5 to 59.9%). NADES comprising lactic acid:glucose:H2O (5:3:1) provided the lowest contents of all elements, and the highest extracted amounts were obtained employing water contents of 60–80%. The calculated daily intake of all the elements contained in NADES extracts were less than the daily dose risk estimators. The hazard quotients, hazard indexes, and carcinogenic risk calculated for all trace elements and their combination were considerably less than 1. This evidences no health risk, and carcinogenic risk after topical application of all studied NADES. For the first time, the results of the current study demonstrated that NADES extracts of F. vesiculosus contain a lower amount of trace metals and are safer than the extracts obtained with water and 70% acetone. This indicates a significant advantage for NADES compared with the other solvents. 相似文献
192.
IMAGING DIAGNOSIS — MAGNETIC RESONANCE IMAGING OF INTRACRANIAL INFLAMMATORY FIBROSARCOMA IN A MIXED BREED DOG
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Elena Scarpante Viktor Palus Brian Alan Summers Abby Caine Giunio Bruto Cherubini 《Veterinary radiology & ultrasound》2016,57(1):E5-E9
An 8‐year‐old mixed‐breed dog presented with progressive behavioral changes and altered mentation. Magnetic resonance imaging (MRI) of the brain revealed an olfactory and frontal lobe extra‐axial mass. The mass exhibited the following MRI signal intensity characteristics: T2W mixed, T1W iso‐ to hypointense, FLAIR hyperintense, and strong contrast enhancement. The mass was removed with cavitronic ultrasonic surgical aspirator (CUSA) assisted neurosurgery. Based on histopathological appearance and immunohistochemistry, the diagnosis of inflammatory fibrosarcoma was made. To our knowledge, this is the first report describing MRI characteristics of intracranial inflammatory fibrosarcoma in the veterinary literature. 相似文献
193.
Katia Varello Marzia Pezzolato Daniela Mascarino Francesco Ingravalle Maria Caramelli Elena Bozzetta 《Journal of veterinary diagnostic investigation》2008,20(2):164-169
Rapid diagnosis of tuberculosis in cattle reacting positive in antemortem assays is crucial in countries where eradication programs are operated to confirm the presence of the infection in tuberculosis-free herds. This study evaluated the accuracy of histopathologic examination by hematoxylin and eosin and Ziehl-Neelsen (ZN) staining applied in this framework, when suspected lesions are caused by low infectious doses and are detected in early stages of the disease. For this purpose, histologic methods were compared with mycobacterial culture as reference test on suspected lymph node samples from 173 cattle reacting positive in antemortem tests. Histopathology demonstrated high sensitivity (93.4%) and specificity (92.3%), while ZN sensitivity and specificity were respectively 33.9% and 100%. There was good agreement between histopathology and bacterial culture, suggesting that histopathologic examination is a reliable tool for rapid diagnosis in countries where active tuberculosis eradication programs allow the prompt identification and elimination of reactor cattle. Histopathology permits identification of typical mycobacterial lesions and its differentiation from other causes. 相似文献
194.
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196.
Camila Arroyo‐Salvo Francisco Sanhueza Fernanda Fuentes Favin Treuln María Elena Arias Paulina Cabrera Mauricio Silva Ricardo Felmer 《Reproduction in domestic animals》2019,54(2):184-194
Conventional in vitro fertilization has not yet been implemented in the equine species. One of the main reasons has been the inability to develop a culture medium and incubation conditions supporting high levels of stallion sperm capacitation and hyperactivation in vitro. Although different culture media have been used for this purpose, human tubal fluid (HTF) medium, widely used in the manipulation of human and mice gametes, has not been reported so far in stallion sperm culture. The first part of this study aimed to compare HTF and Whitten's media on different stallion sperm quality and capacitation variables. Additionally, the effect of procaine, aminopyridine and caffeine in both media was evaluated on sperm motility parameters at different incubation times. Integrity and destabilization of the plasma membrane were evaluated by merocyanine 540/SYTOX Green (MC540), mitochondrial membrane potential (?Ψm) using tetramethylrhodamine methyl ester perchlorate (TMRM), acrosome membrane integrity by PNA/FITC and tyrosine phosphorylation by P‐tyrosine mouse mAb conjugated to Alexa Fluor® by flow cytometry. Motility parameters were evaluated using the integrated semen analysis system (ISAS®). We found no differences between Whitten's and HTF media and incubation time in terms of sperm viability, uninduced acrosome membrane damage or mitochondrial membrane potential at 30‐ and 120‐min incubation. Membrane fluidity (MC540) increased in both media at 30‐ and 120‐min incubation compared to noncapacitating conditions. Similarly, tyrosine phosphorylation increased in both media in capacitating conditions at 2‐ and 4‐hr incubation compared to noncapacitating conditions. Although procaine showed the best result in terms of sperm hyperactivated motility in both media, aminopyridine also showed parameters consistent with the hyperactivation including an increase in curvilinear velocity and decrease in straightness. In conclusion, HTF medium and aminopyridine equally support capacitation‐related parameters in stallion sperm. 相似文献
197.
Effect of dietary conjugated linoleic acid and monounsaturated fatty acids on productive, carcass and meat quality traits of pigs 总被引:1,自引:0,他引:1
Diana Martin Elena Muriel Elena Gonzalez Javier Viguera Jorge Ruiz 《Livestock Science》2008,117(2-3):155-164
Three levels (0, 1 and 2%) of conjugated linoleic acid (CLA) were combined with two levels (low and high) of monounsaturated fatty acids (MUFA) for pig feeding. Productive, carcass and meat quality traits were studied. Large White ♂ × Landrace × Large White ♀ gilts (n = 288) weighting 70 kg were randomly allotted to 6 different feeding treatments and fed to a final average weight of 107 kg. Loins were taken from 48 animals (8 animals randomly selected from each treatment). No differences due to dietary CLA, MUFA or CLA × MUFA interaction were found on average daily gain, average daily consumption, feed conversion ratio, carcass yield, backfat thickness, loin weight, loin pH and loin colour. A significant increase in intramuscular fat content (p = 0.010) and in saturated fatty acids (SFA) (p < 0.001), and a decrease in MUFA (p = 0.001) and desaturase indices were found as consequence of dietary CLA, regardless the MUFA level. Therefore, dietary CLA, MUFA and their interaction did not influence productive and carcass traits of pigs. However, the use of CLA for swine feeding increased the intramuscular fat content and modified the fatty acid profile, regardless the MUFA level of the diets. 相似文献
198.
Agricultural intensification is a major cause for biodiversity loss. It occurs at field scales through increased inputs and
outputs, and at landscape scales through landscape simplification. Agri-environment schemes (AES) of the European Common Agricultural
Policy (CAP) aim at reducing biodiversity loss by promoting extensification of agricultural practises mostly at field scales.
We present a conceptual model for the relationship between landscape complexity and ecological effectiveness of AES based
on (a) non-linear relationships between landscape complexity and abundance and diversity at field scales and (b) four possible
interactive scenarios between landscape- and field scale effects on abundance and diversity. We then evaluated whether and
how effectiveness of AES interacted with landscape-scale effects of intensification along a landscape complexity gradient
established in central Spain. Pairs of cereal fields with and without AES but with the same landscape context were selected
in three regions differing in landscape complexity. Effectiveness of AES was measured as differences between paired fields
in species richness and abundance of five target groups (birds, grasshoppers and crickets, spiders, bees and plants). Landscape
metrics were measured in 500–m radius circular plots around field centres. Positive, negative and no effects of landscape
complexity on effectiveness of AES were found, suggesting that effects of complexity on effectiveness of AES changes from
positive to negative along gradients of landscape complexity. Effectiveness of AES for improving biodiversity was then constrained
by landscape. Compulsory measures aimed at enhancing or maintaining landscape complexity would enhance the effectiveness of
AES for preserving biodiversity in farmed landscapes.
For M. Díaz: New address from October 2007: Instituto de Recursos Naturales, Centro de Ciencias Medioambientales–CSIC, Serrano
115, Madrid E-28006, Spain 相似文献
199.
Rodents play an important role in rangelands through the engineering of extensive burrow systems, which provides key habitats for many animal and plant species. We have analyzed the long‐term variation in the abundance and distribution of rodent burrows in grazing ecosystems of southern Russia (Kalmykia) under the landscape change from desert to steppe caused by the drastic reduction of livestock after the collapse of the USSR in the early 1990s. We conducted burrow surveys in the “desert” (1980) and “steppe” (2017) periods on 19 3‐km transects. We found considerable changes in burrow abundance and distribution, as well as evidence of desert habitat fragmentation and isolation caused by the expansion of tall‐grass communities. Burrows of the open‐dwelling diurnal ground squirrel (Spermophilus pygmaeus), the dominant and the keystone species during the “desert” period, almost completely disappeared from the rodent burrow network by 2017, indicating significant habitat loss. In contrast, the burrows of the folivorous social vole (Microtus socialis) which was rare in the 1980s, became abundant and ubiquitously distributed. The burrow density of the desert‐dwelling psammophilous midday gerbil (Meriones meridianus) decreased, while the distances between occupied patches increased, indicating desert habitat fragmentation and loss of population connectivity. Burrows of the folivorous tamarisk gerbils (M. tamariscinus) were recorded only sporadically in both 1980 and 2017. The observed changes in the rodent burrow network, the key component of grazing ecosystems, correlate with rodent species ecology and can have long‐term and important consequences for ecosystem functioning. 相似文献
200.
Ordaz-Ochoa Gerardo Juarez-Caratachea Aureliano Pérez-Sánchez Rosa Elena Martínez-Flores Héctor Eduardo Esquivel-Cordova Juvenal Ortiz-Rodríguez Ruy 《Tropical animal health and production》2018,50(7):1671-1682
Tropical Animal Health and Production - The effect of cactus (Opuntia ficus-indica) added to the diet of lactating (21 days of lactation) sows on voluntary feed intake, and its impact on... 相似文献