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161.
Specific breeding for organic systems may help reduce their yield gap relative to conventional systems by exploiting genotype × system (GS) interaction. Likewise, specific breeding for distinct subregions within a region could capitalize on genotype × location (GL) interaction. Grain yield and test weight of common wheat varieties were evaluated under organic and conventional systems in ten locations spanning from northern to southern Italy, with the objectives of: (i) comparing production systems; (ii) investigating the extent of GS and GL interactions and their relationship with genotypic and environmental characteristics; and (iii) preliminarily comparing, in terms of predicted selection gains, different strategies to cope with GS and GL effects. These effects were investigated in the 2-year Data set 1 including seven genotypes. GS effects were also assessed in the annual Data sets 2 and 3 including 13 and 11 genotypes, respectively. The yield reduction of organic systems relative to conventional ones averaged 28% in Data set 1, 29% in Data set 2 and 14% in Data set 3. Organic systems also tended to a modest test weight reduction. Genetic correlations between systems ranged from high to very high (0.88 ≤ rg ≤ 0.98) for yield and test weight, owing to nil or limited GS interaction. Broad-sense heritability tended to be higher in conventional systems than organic ones for yield in two data sets (mainly due to lower experimental error) while being similar in the two systems in the other cases. Predicted selection gains suggested nil (yield) or very modest (test weight) advantage of direct selection in organic systems relative to indirect selection in conventional systems, when targeting organic systems. The scope for selection only in conventional systems was reinforced when comparing predicted gains for selection scenarios which target both systems in relation to their foreseeable marketing importance. GL effects for yield and test weight were significant and were modeled by additive main effects and multiplicative interaction analysis. Site classification based on GL effects for yield revealed a larger subregion A including northern and central Italy and a smaller subregion B comprising southern Italy, accordingly with previous, independent studies. Yield selection only in subregion A (with indirect selection gain for subregion B) implied slightly higher predicted gain for A (+4%) and much lower gain for B (−24%) relative to independent, direct selection in each subregion. Selection for specific geoclimatic subregions may have greater importance than selection for specific production systems. 相似文献
162.
The purpose of this research was to study the influence of the vegetation on the soil C pool of forests of pines (Pinus sylvestris) and oaks (Quercus pyrenaica), located in Central-Western Spain. Horizons from selected soils located in these forests were sampled, and the soil organic
C (SOC) was determined. In addition, in vitro incubation experiments were carried out, under controlled conditions, to monitor
the stability of SOC against the microbial activity. Soil humus fractions were isolated following a classical procedure of
chemical fractionation using alkaline solutions, before and after the incubation experiment. A deeper O horizon was found under the pine forest than under oak one; however, higher SOC content was found in the oak site than that
under pine one. During the in vitro mineralization process, a lower CO2 production by the soil sample from pine forest was observed, in relation to that emitted by the oak soil. In addition, a
lower humification degree was estimated for the soil humus under pines than for that under oaks. In conclusion, replacement
of oaks by pines produced a decrease in SOC accumulation and a lower quality of humus in the forest soils. 相似文献
163.
Elena Marcos Leonor Calvo José Antonio Marcos Ángela Taboada Reyes Tárrega 《European Journal of Forest Research》2010,129(1):25-30
We aimed to study tree effects on the chemical properties of forest soils. We compared soil features of three types of forest ecosystems, each with four stands (replicates): beech forests (Fagus sylvatica), oak forests (dominated by Quercus pyrenaica) and pine plantations (Pinus sylvestris). Five samples from the top 10 cm of soil were taken per stand, from which pH, organic matter content (O.M.), total nitrogen (N) and available calcium (Ca2+), magnesium (Mg2+), potassium (K+) and sodium (Na+) were determined. Litter layer depth was measured at each soil sampling point. We also measured tree density and crown diameters at each stand. Our results indicated that soil samples from the four pine plantation stands were more similar while oak and beech stands were characterised by great variability in terms of soil properties and leaf litter depth. Although the identity of the dominant tree species significantly influenced several topsoil chemical properties (increase in pH and available cations in oak forests and higher organic matter and total nitrogen in beech and pine ecosystems), there were other important factors affecting soil features that may be taken under consideration. Differences between soil properties of the three types of forest ecosystems were mainly related to the characteristics of the litter layer and less related to the tree layer structure. Finally, the establishment of pine plantations in naturally deciduous tree areas made the topsoil features more homogeneous. 相似文献
164.
Elena Fringuelli Lorena Urbanelli Omar Tharuni Patrizia Casagrande Proietti Annalisa Bietta Irit Davidson Maria Pia Franciosini 《Research in veterinary science》2010,89(3):367-372
This is the first paper describing the cloning of pigeon IFN-γ gene (PiIFN-γ) and the analysis of the in vitro expressed recombinant protein. The PiIFN-γ gene was identified by RT-PCR as a 498 bp, fragment coding for a precursor protein of 165 amino acids instead of 164 amino acids, as observed in the other avian species. The recombinant protein was expressed in vitro by an eukaryotic system and the biological properties of the cytokine were tested using a chicken macrophage cell line. The high degree of amino acid and nucleotide identity, shared with the ChIFN-γ, and the fact that the pigeon protein was functional on chicken cells, indicates a cross-reactivity between pigeon and chicken IFN-γ. The detection of the PiIFN-γ could represent an useful instrument in understanding the role played by this cytokine in immune response related to vaccinations and infectious diseases in the pigeon. 相似文献
165.
Buim MR Buzinhani M Yamaguti M Oliveira RC Mettifogo E Timenetsky J Ferreira AJ 《Comparative immunology, microbiology and infectious diseases》2010,33(1):15-23
Mycoplasma synoviae (MS) is an important avian pathogen may cause both respiratory disease and joint inflammation synovitis in poultry, causing economic losses to the Brazilian poultry industry. The genotypic variation in 16S rRNA gene is unknown. Partial sequences of 16S rRNA gene of 19 strains of M. synoviae were sequenced and analyzed in order to obtain molecular characterization and evaluation of the genetic variability of strains from distinct Brazilian areas of poultry production. Different polymorphic patterns were observed. The number of polymorphic alterations in the studied strains ranged from 0 to 6. The nucleotide variations, including deletion, insertion and substitutions, ranged from 3 to 5. The genotypic diversity observed in this study may be explained by spontaneous mutations that may occur when a lineage remains in the same flock for long periods. The culling and reposition in poultry flocks may be responsible for the entry of new strains in different areas. 相似文献
166.
167.
Biostimulant activity of two protein hydrolyzates in the growth and nitrogen metabolism of maize seedlings 总被引:2,自引:0,他引:2
Andrea Ertani Luciano Cavani Diego Pizzeghello Elena Brandellero Adriano Altissimo Claudio Ciavatta Serenella Nardi 《植物养料与土壤学杂志》2009,172(2):237-244
Two protein hydrolyzate–based fertilizers (PHFs), one from alfalfa (AH) and one from meat flour (MFH), were studied chemically and biologically. AH and MFH revealed a different degree of hydrolysis and a different amino acid composition. The biostimulant activity was investigated using two specific and sensitive bioassays of auxins and gibberellins. Extracts of AH and MFH elicited a gibberellin‐like activity and a weak auxin‐like one. To improve our understanding of the biostimulant activity, AH and MFH were supplied to maize plants and their effect on growth and nitrate metabolism was studied. Both PHFs increased root and leaf growth and induced morphological changes in root architecture. Besides, the treatments increased nitrate reductase (NR) and glutamine synthetase (GS) activities, suggesting a positive role of the two hydrolyzates in the induction of nitrate conversion into organic nitrogen. Moreover, treatments enhanced GS1 and GS2 isoforms in maize leaves. The latter isoform, amounting to 5‐ to 7‐fold the level of the former, appears to be a superior form in the assimilation of ammonia. The high NR and GS activities together with the high induction of GS isoforms indicate a stimulatory effect of the two PHFs on the assimilation of nitrate. In addition, a role of amino acids and small peptides of the two PHFs is suggested in the regulation of the hormone‐like activity and nitrogen pathway. 相似文献
168.
The impact of storm water runoff on a small urban stream 总被引:2,自引:0,他引:2
Vilmantė Karlavičienė Sigita Švedienė Danutė Elena Marčiulionienė Peter Randerson Mindaugas Rimeika William Hogland 《Journal of Soils and Sediments》2009,9(1):6-12
Background, aim, and scope In urban areas, storm water runoff often transports various pollutants, some of which settle and form sediments. In order
to have the comprehensive view of the ecological state of storm water runoff recipients, both water and sediments of the stream
must be assessed. In the Baltic Sea Area, the Water Framework Directive & HELCOM Recommendations aim to prevent or minimise
pollution caused by harmful substances arising from storm water runoff, in order to promote the ecological restoration of
the Baltic Sea—one of the most vulnerable seas. The aim of the study was to investigate the toxicity of bottom sediments of
a small storm water runoff recipient focusing on the potential impact of successive discharges of urban storm water. Some
storm water runoff quality parameters and the toxicity of bottom sediments of recipients was studied in this research.
Materials and methods During 9 years, at four discharge points, minimum four grab samples per year at each discharge point were taken for chemical
characterisation. General parameters (pH, SS, BOD7, CODCr and TPH) in liquid phase samples were analysed according to standard methods. Annual limit values were taken from the Lithuanian
EPA requirements for the management of storm water runoff with a focus on prevention and control of contamination. Eleven
composite samples of stream bottom sediments, each consisting of ten sub-samples, were collected in 2006. Toxicity screening
from sediments was performed using the plant Lepidium sativum according to modified I. Magone’s methodology (Magone I, Bioindication of phytotoxicity of transport emission. In: Kachalova
O-L, Zinatne (eds) Bioindication of toxicity of transport emissions in the impact of highway emissions on natural environment. Riga, pp 108–116, 1989). The level of toxic impact of Lepidium sativum (compared to control) was assessed according to the modified method of Wang (Rev Environ Contam Toxicol 126:88–127, 1992).
Results The mean pH of urban storm water runoff does not vary much from neutral, but range values are quite different, from 4.0 up
to 8.7. The highest concentration of SS reached 800 mg L−1, TPH—2.4 mg L−1, BOD7—300 mg O2 L−1 and CODCr—1,400 mg L−1. The SS was above the limit in 64% of total amount of grab samples, TPH—37%, BOD7—41% and CODCr—55%. The toxicity analysis of the bottom sediments showed varying toxicity of bottom sediments along the stream. From nine
analysed samples of bottom sediments, 30% had weak toxicity, 30% medium and 30% strong toxicity on the test organism plant
L. sativum. There was one single sample with no toxic effects, so that the results showed that urban storm water has an unacceptable
environmental impact on recipients. It was also indicated that storm water runoff discharge alone is not the potential source
of toxicity of bottom sediments. The litter demonstrated a weak toxicity of bottom sediments as well.
Discussion Most local authorities do not consider storm water runoff discharges to be a matter of great concern because they believe
that surface runoff arising from rainfall is still relatively clean. The study showed that the current method of monitoring
storm water runoff quality by chemical analyses is not the best tool for environmental impact assessment and must be combined
with toxicity tests of bottom sediments of recipients.
Recommendations and perspectives To avoid the environmental impact of storm water runoff more attention should be paid to the development and implementation
of storm water runoff pollution prevention measures. The study implies that future research concerning the relationships between
storm water runoff deposit characteristics and biological activities must be developed to evaluate the contamination potential
of stream sediment deposits for local aquatic ecosystems. Further studies should be developed to characterise the activities
of the microbial community of storm water runoff sediments, and to monitor bioremediation in situ. 相似文献
169.
M Gil N Kontoudakis E González M Esteruelas F Fort JM Canals F Zamora 《Journal of agricultural and food chemistry》2012,60(32):7988-8001
The aim of this paper was to study how maturity and maceration length affect color, phenolic compounds, polysaccharides, and sensorial quality of Cabernet Sauvignon and Tempranillo wines at three stages of grape ripening. Ripeness increased color extractability, phenolic compounds, and polysaccharide concentrations. Moreover, the proanthocyanidin mean degree of polymerization (mDP) and the percentage of prodelphinidins also increased with maturity, whereas the percentage of galloylation decreased. In general, wines from riper grapes contain higher proportions of skin proanthocyanidins. Color and anthocyanin concentration decreased when the maceration was longer, whereas polysaccharide and proanthocyanidin concentrations did the opposite. It was also detected that the mDP and the percentage of prodelphinidins decreased when the maceration was extended, whereas the percentage of galloylation increased. These data seem to indicate that proanthocyanidin extraction from seeds is clearly increased throughout the maceration time. 相似文献
170.
Elena Bernalte Carmen Marín Sánchez Eduardo Pinilla Gil Francisco Cereceda Balic Víctor Vidal Cortez 《Water, air, and soil pollution》2012,223(8):5143-5154
A set of 72?PM10 samples from low-polluted urban and rural locations belonging to the regional air monitoring network of Extremadura (Spain) were collected in a 1?year sampling period. Sample pre-treatment and analytical determination by gas chromatography?Cion trap mass spectrometry were optimised and validated for the analysis of the priority 16 US Environmental Protection Agency polycyclic aromatic hydrocarbons (PAHs). The influence of meteorological conditions (temperature, relative humidity and solar radiation) and other atmospheric pollutants (O3, NO2, SO2, PM10) has been covered in detail and Pearson correlation test were used for this purpose. Spatial distribution of particulate PAHs was evaluated and the comparison with other European sites was also established. Possible emission sources were identified and assigned by using molecular diagnostic criteria. 相似文献