Does Cattle Grazing Affect Ant Abundance and Diversity in Temperate Grasslands?

Half of the world's land base is grazed by domesticated livestock. Because of the important functional role of ants in grasslands, it is important to understand the effect of livestock grazing on ant abundance and diversity. The objectives of this study were to examine the effect of cattle grazing and site productivity on the abundance, species richness, and species diversity of ants in Lac du Bois Grasslands Provincial Park, British Columbia, Canada. We hypothesized that the measured ant variables would be lowest in grazed areas and at low site productivity. Pitfall trapping was conducted at four sites: two at each low and high site productivity levels. At each site an ungrazed (fenced exclosure) and grazed transect was sampled during May, July, and August of 2008. Captured ants were preserved in ethanol and identified. Eight genuses of ants were collected: Tapinoma, Camponotus, Formica, Lasius, Aphaenogaster, Myrmica, Solinopsis, and Temnotharox. The mean number of ants per pitfall was higher at high site productivity sites that were grazed (15.10 ±  SE) compared to high productivity sites ungrazed (3.28 ±  SE); grazing at low productivity reduced numbers of ants from 5.07 (± 0.70 SE) to 2.20 (± 0.39 SE) (F = 21.806; P [ 0.001). Tapinoma sessile and A. occidentalis had the greatest numbers in the pitfall traps. Species richness (F = 23.330, P [ 0.001) and diversity (F = 11.764, P = 0.001) followed a similar trend. Because productivity and cattle grazing affect ant diversity and abundance, and ants impact ecosystem functioning, these factors should be considered in management of grasslands.     

Population biology and epidemiology of plant virus epidemics: from tripartite to tritrophic interactions

Natural enemies have long been used in biological control programs to mitigate the damage caused by herbivory. Many herbivorous insect species also act as plant virus vectors, enabling virus transmission from plant to plant and hence disease development in a plant population. Whilst an intuitive assumption would be to expect a decrease in vector numbers to lead to subsequent reductions in virus transmission, recent evidence suggests that introduction of natural enemies (parasitoids and predators) may in some cases increase plant virus transmission while at the same time reducing vector numbers. In this paper we review the evidence for plant-virus-vector-natural enemy interactions, the signalling mechanisms involved and their implications for virus transmission, and show how a modelling approach can assist in identifying the key parameters and relationships involved in determining the disease outcome. A mathematical model linking the population dynamics of a vector-parasitoid system with virus transmission was used to investigate the effects of virus inoculation and acquisition rates, parasitoid attack rate and vector aggregation on disease dynamics across a wide range of parameter value combinations. Virus spread was found to increase with enhanced inoculation, acquisition and parasitoid attack rate but decrease with high levels of vector aggregation.     

Digestible energy intake, dry matter digestibility and mineral status of grazing New Zealand Thoroughbred yearlings

AIM: To measure the nutritive value of pasture in terms of digestible energy intake (DEI) and dry matter (DM) digestibility, and to determine the effect of Ca, P, Cu, Zn, and Se supplementation on growth rate and degree of physeal swelling in Thoroughbred yearlings grazed on pasture. METHODS: Fourteen yearling horses were randomly allocated to 1 of 2 groups and rotationally grazed on a ryegrass/white clover pasture for 7 months. One group was supplemented daily with a mineral mix. Liveweight changes were recorded at fortnightly intervals and pasture mineral composition determined at monthly intervals. The DM intake (DMI) was determined from daily faecal DM outputs divided by the indigestible DM fraction (1 - digestible DM) determined from a digestibility study. The DEI was determined from the difference between the gross energy intake and the gross energy faecal output. The DM, gross energy content, crude protein (CP), soluble carbohydrate, acid detergent fibre (ADF), neutral detergent fibre (NDF), lipid and mineral composition of pasture offered and faeces were analysed and their digestibility or apparent absorption calculated. RESULTS: The DM digestibility of pasture averaged 0.64 while the daily DMI and DEI of a 350 kg yearling, gaining 0.6 kg liveweight/day, averaged 6.9 kg DM/day and 78 MJ DE/day, respectively. The mineral supplement had no significant effect on the growth of yearlings grazing pasture that had a mineral composition of (g/kg DM) Na 0.17, K 32.8, Ca 3.6, P 3.4, Mg 2.1, S 3.0, and (mg/kg DM) Cu 7.9, Fe 177, Mn 83, Zn 28 and Se 0.016. Plasma Se was elevated (e.g. 40-218 microg/l) by Se supplementation while Cu supplementation had no effect on plasma and liver Cu concentrations. Physeal swelling was observed in all horses, regardless of treatment. CONCLUSION: Good quality ryegrass/white clover pasture that had a DE content of 11.3 MJ/kg DM and adequate mineral composition (as observed in this study, with the possible exception of Se) will provide an adequate intake of nutrients to ensure good growth in yearling horses.     

Visceral leishmaniasis in a New York foxhound kennel

Although endemic throughout much of the world, autochthonous visceral leishmaniasis has been reported on only 3 previous occasions in North America. After diagnosis of visceral leishmaniasis in 4 foxhounds from a kennel in Dutchess County, New York (index kennel), serum and ethylenediamine-tetraacetic acid (EDTA)-anticoagulated blood were collected from the remaining 108 American or cross-bred foxhounds in the index kennel and from 30 Beagles and Basset Hounds that were periodically housed in the index kennel. Samples were analyzed for antibodies to or DNA of tickborne disease pathogens and Leishmania spp. Most dogs had antibodies to Rickettsia spp., Ehrlichia spp., Babesia spp., or some combination of these pathogens but not to Bartonella vinsonii (berkhoffi). However, DNA of rickettsial, ehrlichial, or babesial agents was detected in only 9 dogs. Visceral leishmaniasis was diagnosed in 46 of 112 (41%) foxhounds from the index kennel but was not diagnosed in any of the Beagles and Basset Hounds. A positive Leishmania status was defined by 1 or more of the following criteria: a Leishmania antibody titer > or = 1:64, positive Leishmania polymerase chain reaction (PCR), positive Leishmania culture, or identification of Leishmania amastigotes by cytology or histopathology. The species and zymodeme of Leishmania that infected the foxhounds was determined to be Leishmania infantum MON-1 by isoenzyme electrophoresis. Foxhounds that were > 18 months of age or that had traveled to the southeastern United States were more likely to be diagnosed with visceral leishmaniasis. Transmission of Leishmania spp. in kennel outbreaks may involve exposure to an insect vector, direct transmission, or vertical transmission.     

Use of immunochemical techniques for the analysis of pesticides

Immunochemical assays for small molecules such as pesticides are rapidly gaining acceptance among analytical chemists. These techniques are rapid, sensitive, cost effective and can easily cope with large sample loads. This review lists the advantages and disadvantages of the technique and describes the steps in assay development using examples from this laboratory, particularly the thiocarbamate herbicide molinate and the triazine herbicides. The focus is primarily on hapten synthesis strategies, assay format considerations, sample preparation and assay validation.     

Verbena × hybrida flower volatiles attractive to Western flower thrips,Frankliniella occidentalis

Western flower thrips (WFT) are attracted to three flowering verbena cultivars. The volatile components of these cultivars contain different enantiomers of linalool oxide which have been synthesised and one shown to be attractive to WFT.