Brooding temperatures for chicks acclimated to heat during incubation: effects on post-hatch intestinal development and body weight under heat stress

1.?The objective was to evaluate the effects of brooding temperature on intestinal development, oxidative organ damage, and performance of chicks acclimated to high temperature during incubation. The effects of acclimation and brooding temperatures on slaughter weights of broilers under heat stress were also investigated.

2.?Eggs were incubated at either 37?8°C (INC_Cont) or heat-acclimated at 39?5°C for 6 h daily from d 10 to d 18 of incubation (INC_H). Brooding temperatures at floor level were set at 32, 33?5 and 35°C (Bt₃₂, Bt_33?5, Bt₃₅, respectively) for the first 5 d. The temperature was reduced gradually to 30°C from d 6 to d 10. From 21 to 42 d, broilers from INC_Cont Bt₃₂ and INC_H Bt₃₂ and Bt₃₅ were divided into two groups; half from each group was exposed to daily cyclic higher ambient temperatures, while the other half was reared at control temperature.

3.?INC_H chicks had lower jejunum, but greater liver and residual yolk sac weights than INC_Cont chicks on the day of hatching. Although INC_H chicks from Bt_33?5 and Bt₃₅ had lighter body weights than Bt₃₂ on d 5, no significant differences were observed in the body weight of broilers among treatments at 10 and 21 d.

4.?Similar jejunum protein, alkaline phosphatase, maltase, glutathione, and malondialdehyde contents of chicks from INC_Cont and INC_H suggested that heat acclimation during incubation has no effect on jejunum enzyme activity or oxidative status of chicks.

5.?Taking into account INC_H Bt₃₅ chicks having lower T₃ levels on d 5, lower heterophil/lymphocyte (H/L) ratios and similar weights at 42 d under heat stress compared with control broilers, the results suggested that although higher brooding temperatures had no effect on body weights of INC_H chicks during the brooding period, those broilers may able to cope better with heat stress.     

Five-year effects of cutting and herbicide treatments on control of <Emphasis Type="Italic">Rhododendron flavum</Emphasis> Don., and macronutrient pools in eastern beech (<Emphasis Type="Italic">Fagus orientalis</Emphasis> Lipsky) forests of Turkey

This experiment was carried out in a mature (>100-year-old) eastern beech forest in the Eastern Black Sea Region (BSR) of Turkey. The effects of experimental practices to control the woody plant Rhododendron flavum Don., including grubbing, manual cutting, and foliar and cut-stump spraying with imazapyr and triclopyr, were assessed on understory and forest floor biomass and nutrients, as well as soil bulk density and nutrient content. The cut plots had 5, 15, and 38 times greater competing vegetation biomass than did the plots treated with triclopyr, grubbing, and imazapyr, respectively. Because of the increased biomass, the amount of understory N, P, Ca, K, S, and Mg content on the cut plots was also substantially higher when compared to the grubbed, imazapyr-treated, and triclopyr-treated plots. Triclopyr and imazapyr applications brought about two- and threefold greater dead organic matter on the forest floor than did cutting and grubbing, respectively. As a result, forest floor macronutrient contents on the imazapyr- and triclopyr-treated plots were significantly greater than on the cut plots. Five years after treatments, soil bulk density at the 10–20 cm depth on the imazapyr-sprayed plots was about 14% higher than that on other treatments. Herbicides, especially imazapyr and grubbing were effective in controlling rhododendron. Macronutrients stored in the rhododendron were released to the forest floor after vegetation was controlled with herbicides.     

Production of human milk fat analogue containing docosahexaenoic and arachidonic acids

Human milk fat (HMF) analogue containing docosahexaenoic acid (DHA) and arachidonic acid (ARA) at sn-1,3 positions and palmitic acid (PA) at sn-2 position was produced. Novozym 435 lipase was used to produce palmitic acid-enriched hazelnut oil (EHO). EHO was then used to produce the final structured lipid (SL) through interesterification reactions using Lipozyme RM IM. Reaction variables for 3 h reactions were temperature, substrate mole ratio, and ARASCO/DHASCO (A:D) ratio. After statistical analysis of DHA, ARA, total PA, and PA content at sn-2 position, a large-scale production was performed at 60 °C, 3:2 A:D ratio, and 1:0.1 substrate mole ratio. For the SL, those results were determined as 57.3 ± 0.4%, 2.7 ± 0.0%, 2.4 ± 0.1%, and 66.1 ± 2.2%, respectively. Tocopherol contents were 84, 19, 85, and 23 μg/g oil for α-, β-, γ-, and δ-tocopherol. Melting range of SL was narrower than that of EHO. Oxidative stability index (OSI) value of SL (0.80 h) was similar to that of EHO (0.88 h). This SL can be used in infant formulas to provide the benefits of ARA and DHA.     

A modified enzymic browning assay for potato glandular trichomes

Tetralobulate (Type A) glandular trichomes are present on foliage of many wild as well as cultivated species of potatoes. These trichomes confer resistance to insect pests if their density, gland size and biochemical products are similar as those ofSolanum berthaultti Hawkes andS. polyadenium Greenm. Hybrids ofS. tuberosum L. ×S. berthaultii have been screened for resistance by measurement of phenolic oxidation activity in their Type A trichomes by use of an enzymic browning assay. However, variability in the procedure has prompted the development of an improved assay.     

Effect of different housing systems on fattening performance,slaughter and carcass characteristics of Akkeçi (White Goat) male kids

The aim of this study was to determine the fattening performance and the slaughter and carcass characteristics of Akkeçi (Saanen × Kilis (B₁) crossbred) male kids raised in individual crates (IC) or in group pen housing (GP). In both GP and IC groups, total 13 heads of male kids, whose initial body weights were 21.43 ± 1.03 kg and 20.61 ± 0.98 kg, respectively, evaluated for their fattening performances. After 56 days of the fattening period, the final body weights and average daily weight gains of the GP and IC kids were recorded as 30.84 ± 0.82 kg and 25.84 ± 0.76 kg; 182.42 ± 14.77 g and 92.09 ± 13.76 g, respectively (P < 0.001). Adrenal gland weights of the GP and IC groups were similar as 1.66 ± 0.25 g and 1.77 ± 0.22 g, respectively. Although, the carcass conformation of kids was similar between groups, there were significant differences between GP and IC groups for some slaughter and carcass characteristics. In general, although housing kids reared in individual crates provides opportunity to individually feed and monitor a kid, the results obtained from the individual crates in the present study were not satisfactorily from the ethological and production points of view.     

Origin, course and distribution of the hypoglossal nerve in the New Zealand rabbit (Oryctolagus cuniculus L)

With 8 figures SUMMARY: This study aimed at revealing the origin, course and distribution of the hypoglossal nerve in 20 adult male New Zealand rabbits. In all the animals dissected, the hypoglossal nerve arose from the ventrolateral side of the medulla oblongata with two main roots and gave off a descending branch to the ansa cervicalis before reaching the division of the common carotid artery. This branch was not seen on the right side of only one case. At the lateral aspect of the hyoglossus muscle, the nerve then divided into the lateral and medial main branches, sent branches to the styloglossus, hyoglossus, genioglossus and geniohyoideus muscles and terminated in the intrinsic tongue muscles. A communicating branch was observed between the hypoglossal and accessory nerves in the right side of one animal and between the hypoglossal nerve and the ganglion nodosum in the right retropharyngeal area of another animal. An additional branch was observed innervating the stylohyoideus muscle in one animal only. A lateral lingual-hypoglossal communication was also seen between the lateral branch of the hypoglossal nerve and terminal branches of the lingual nerve.     

Evaluation of haemodynamic changes of uterine arteries using Doppler ultrasonography during different stages of pregnancy in Bos indicus cows

The objectives of the study were to evaluate haemodynamic changes and their relationships among ipsilateral (IPS) and contralateral (CONT) uterine arteries (UA) during different stages of pregnancy in Bos indicus cows. Multiparous pregnant cows (n = 40) having a gestation length 30.47 ± 0.54 (mean ± SD) days were randomly enrolled and subjected to Doppler ultrasonography sequentially at 1st, 2nd, 4th, 6th and 8th months of gestation. Blood flow indices including diameter of UA (mm), blood flow volume (BFVo, ml/min), blood flow velocity (BFVe, cm/s), time-averaged maximum velocity (TAMV, cm/s), pulsatility index (PI) and resistance index (RI) were recorded. Data were analysed with mixed models using the PROC MIXED procedures, and Pearson correlation coefficients were calculated using the PROC CORR statement in SAS. The final statistical models included the fixed effects of side of UA, gestation month and the interaction between side of UA and gestation month. Results revealed that the mean diameter of the UA (12.13 ± 0.22 vs. 10.09 ± 0.22), BFVo (1236.33 ± 0.55 vs. 770.41 ± 0.55), BFVe (17.18 ± 0.42 vs. 15.58 ± 0.42) and TAMV (17.11 ± 0.44 vs. 15.77 ± 0.44) was higher (p < .05) in IPS as compared to CONT side of the UA in cows. However, PI and RI did not differ between IPS and CON arteries of uterus in cows. A very high and positive correlation (r = .89; p < .05) existed between the diameter of UA and BFVo starting from 1st to 8th months of gestation in IPS as well as CONT sides of UA. Moreover, TAMV was highly and positively correlated (r = .91; p < .05) with BFVe throughout the gestation. In conclusion, these haemodynamic changes in the UA could be used as a valuable validity tool to differentiate the compromised pregnancy in Bos indicus cows.     

Morphometric analysis of the mandible of Bardhoka autochthonous sheep in Kosovo

Kosovo, one of the countries in the Balkan Peninsula, has a long tradition in sheep farming, and sheep breeds share triple purpose breed characteristics: milk, meat and wool. Bardhoka sheep, also with triple productive profile, is an important sheep breed because of its economic value with its milk production. In this study, 24 mandibles of adult Bardhoka sheep (12 male and 12 female) aged between 2 and 5 were used. The sheep were acquired from slaughterhouse around Kosovo, and the mandibles were selected without any deformities from healthy sheep. After cleaning the mandibles, a total of 12 morphometric measurements were taken from each sample using digital calliper. The difference between female and male was statistically significant (p < .05) for measurement 11 (height of mandible level of alveolar edge of 3rd molar tooth). The mean value of measurement 11 in male individuals was 42.26 ± 3.96, and it had a higher value than in female individuals (39.37 ± 2.57). In the literature, knowledge related to mandible morphometry of Bardhoka sheep was insufficient. The aim of this study was to determine mean values from osteometric measurements of Bardhoka sheep mandible and obtain references with the values acquired.     

Effect of humic substances in nutrient film technique on nutrient uptake

An experiment was conducted to find out how humic substances affected nutrient uptake of plants. The test plants, oregano, thyme, and basil, were grown in nutrient film technique at two pH levels (4.5 and 6.5), in two substrates (peat and perlite), and at three levels of humic substance that was a peat extract (control, low, and high concentration). Nutrient uptake of potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) were determined by elemental contents in aerial parts of the plant and its weight. Humic substance had no effect on K, Ca, and Mg uptake but lowered the uptake of Fe, Mn, Zn, and Cu for the three test plants, more pronounced with perlite than peat and more at low pH than at high pH. The lowering of the uptake might be caused by complexation of Fe, Mn, Zn, and Cu by the humic substance and the lower availability of these metals in complexed form than as a cation or as EDTA‐chelate in the case of Fe. It is not clear why the effect of the humic substance on micro‐element uptake is larger at low than at high pH. The complexation is expected to be stronger at pH 6.5 than at pH 4.5. At low pH, the high concentration of humic substance caused a low fresh weight of the shoots, perhaps caused by a toxicity of the humic substance at low pH. This was less pronounced at high pH.     

THE EFFECT OF NITROGEN SOURCES ON YIELD AND QUALITY OF SALAD ROCKET GROWN IN DIFFERENT MONTHS OF THE YEAR

The effects of months of the year and nitrogen (N) sources on salad rocket (Eruca sativa Mill.) yield, quality, and nitrate accumulation was investigated during the years 2002 and 2003. In both years, seeds were sown on the first day of April, May, June, July, August and September. Three different nitrogen sources were used: farmyard (cattle) manure (100 tonnes·ha^?1), calcium nitrate [Ca(NO₃)₂]-15.5% N (150 kg N·ha^?1) and ammonium sulfate [(NH₄)₂SO₄)]-21% N (150 kg N·ha^?1). Yield, leaf color, dry matter, vitamin C and total glucosinolate content and nitrate accumulation was assessed. Growing months affected all the assessed parameters significantly both years, with the exception of hue angle in 2003, whereas nitrogen source only influenced yield and nitrate accumulation in 2003. In both years the highest yield was obtained in April, but vitamin C and total glucosinolate contents were higher during summer months, and leaves were slightly darker colored. Chemical fertilizers increased the yield compared to farmyard manure; however, they also increased nitrate accumulation slightly, without any significant difference between them. Nitrate accumulation never exceeded 300 mg kg^?1 fresh weight (FW), which is well below the acceptable daily intake of 3.7 mg nitrate per kg^?1 bodyweight set by European Commission's Scientific Committee on Food.