Seroprevalence of malignant catarrhal fever-related gammaherpesviruses in domestic ruminants in Turkey

The prevalence of malignant catarrhal fever (MCF) virus infection in cattle, sheep and goat populations and also the prevalence of recovered and chronic MCF cases in north-western Turkey are reported. A total of 600 animals, 200 individuals of each species, located in four provinces were sampled between December 2003 and July 2005. A monoclonal antibody-based competitive inhibition (ci) ELISA were used to detect infection status of the animals. Detected antibody prevalence was 97.5%, 96.0% and 15.0% in sheep, goats and cattle, respectively. These results showed that MCF related gammaherpesvirus infections are common in north-western Turkey. There was no significant difference between prevalences detected in sheep and goats, as well as various breeds of these species. There was also no significant difference among locations. Results of this study show that sheep and goats may equally be important in the epidemiology of MCF in Turkey. Seropositivity against MCF agents among cattle was 15.0%. The results indicate that MCF infections may be maintained in intensively managed cattle herds having no close contact with small ruminants.     

A Field Survey of Promising Mulberry (M<Emphasis Type="Italic">orus spp</Emphasis>.) Genotypes from Turkey

In this study the mulberry population in the Mediterranean and Eastern Anatolia Turkey was examined with regard to fruit properties. The research material consisted of uncultivated mulberry (Morus spp.) trees. Thirty five mulberry genotypes for table, 6 genotypes for fruit juice, 11 genotypes for syrup (pekmez) and 9 genotypes for drying, were selected, 32 of which belonged to Morus alba, 12 to Morus rubra and 8 genotypes to Morus nigra. Continued and more detailed selection studies with this population of genotypes are necessary to provide material that is suitable for future breeding efforts. It may also be possible to select some of this native material that has sufficient commercial value to justify its release to growers.     

Effect of Different Treatments on Branching of Some Apple Trees in Nursery

This study was conducted to determine effect of different treatments on branching of one-year-old ‘Fuji’, ‘Granny Smith’ and ‘Red Delicious, Red Chief’ trees which grafted on M9 rootstock. Plant growth promoting rhizobacteria (Pseudomonas BA-8) strain, 6?Benzyl Adenin (BA) and pinching were used to promote branching. The BA (300?ppm) and BA-8 (10⁹CFU/ml) were applied to first twenty centimeters of the top part of trees. The BA, BA-8 and Pinching was applied to trees at 75?cm in length. According to our results, BA-8 and pinching increased number of branches compared to control and BA treatments for all cultivars. BA-8 increased number of branches in ‘Fuji’, ‘Red Delicious, Red Chief’ and ‘Granny Smith’ from 2.71, 1.70 to 4.25 branches/tree, respectively while pinching increased 1,95 branches/tree in ‘Red Delicious, Red Chief’ and 4,01 branches/tree. The highest branch’s angle was obtained from BA-8 bacteria in ‘Fuji’ (64.46°), and ‘Red Delicious, Red Chief’ (52.08°) and ‘Granny Smith’ (56.91°). BA-8 bacteria treatment was found alternative practice instead of pinching in terms of branching performance.     

Growth performance of bath sponge (Spongia officinalis Linnaeus, 1759) farmed on suspended ropes in the Dardanelles (Turkey)

Commercial bath sponge population in the Mediterranean has been recently reduced due to diseases and the depletion of natural banks. The commercial supply is far below the demand on the last 10–15 years. In this study, we aimed to investigate the farming performance of Spongia officinalis in the Dardanelles. Sponges collected from the Dardanelles cut into pieces of different weight. They were divided into two groups depending on the wet weight ranging from 50 to 150 g (mean weight 102.6 ± 4.81 g) and from 160 to 360 g (mean weight 235.8 ± 8.56 g). Three rope systems located parallel to each other with 6–7 m inter‐distance were constructed for the cultivation of sponges. At the end of the study, while small‐sized sponges could reach mean wet weight of 120.7 ± 14.8 g, large‐sized sponges reached mean wet weight of 247.6 ± 22.4 g. Growth rates were estimated 5–17% at 21st month. The survival rates were found 82% and 88%.     

Mycobacteria in aquarium fish: results of a 3‐year survey indicate caution required in handling pet‐shop fish

Fish are commonly infected with non‐tuberculous mycobacteria (NTM), which should be regarded as potential pathogens when handling aquarium fish and equipment. This study examined 107 aquarium fish from pet shops. Cultivation of the fish samples using different selective media was conducted for identification of NTM. Isolates were identified using the GenoType Mycobacterium common mycobacteria and additional species assays, sequencing of the 16S rRNA and rpoB genes, and real‐time PCR assay for identification of Mycobacterium (M.) marinum. Among the investigated fish, 79.4% (85/107) were positive for mycobacteria, with 8.2% (7 of 85) having two mycobacterial species present. Among the positive fish, the common pathogens M. marinum, Mycobacterium fortuitum (M. fortuitum group) and Mycobacterium chelonae were identified in approx. 90% of fish and other NTM species in 10%, including Mycobacterium peregrinum/septicum, Mycobacterium gordonae, Mycobacterium arupense, Mycobacterium kansasii, Mycobacterium ulcerans and Mycobacterium setense. The well‐known human pathogen M. marinum was present in 10.6% of the positive fish (9 of 85). The species of mycobacteria identified in the study are not only recognized as aquarium fish pathogens, but can also cause pathology in humans. Microbiological and clinical communities should therefore be sensitized to the role of NTM in infections associated with exposure to aquarium fish.     

Antimicrobial and antioxidant activity of the essential oil and methanol extracts of Thymus pectinatus Fisch. et Mey. Var. pectinatus (Lamiaceae)

The essential oil, obtained by using a Clevenger distillation apparatus, and water-soluble (polar) and water-insoluble (nonpolar) subfractions of the methanol extract of Thymus pectinatus Fisch. et Mey. var. pectinatus were assayed for their antimicrobial and antioxidant properties. No (or slight) antimicrobial activity was observed when the subfractions were tested, whereas the essential oil showed strong antimicrobial activity against all microorganisms tested. Antioxidant activities of the polar subfraction and the essential oil were evaluated using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, superoxide radical scavenging, and lipid peroxidation assays. The essential oil, in particular, and the polar subfraction of the methanol extract showed antioxidant activity. The essential oil was analyzed by GC/MS, and 24 compounds, representing 99.6% of the essential oil, were identified: thymol, gamma-terpinene, p-cymene, carvacrol, and borneol were the main components. An antimicrobial activity test carried out with fractions of the essential oil showed that the activity was mainly observed in those fractions containing thymol, in particular, and carvacrol. The activity was, therefore, attributed to the presence of these compounds. Other constituents of the essential oil, such as borneol, gamma-terpinene, and p-cymene, could be also taken into account for their possible synergistic or antagonistic effects. On the other hand, thymol and carvacrol were individually found to possess weaker antioxidant activity than the crude oil itself, indicating that other constituents of the essential oil may contribute to the antioxidant activity observed. In conclusion, the results presented here show that T. pectinatus essential oil could be considered as a natural antimicrobial and antioxidant source.     

Use of Onion (Allium cepa) and Garlic (Allium sativum) Wastes for the Prevention of Fungal Disease (Saprolegnia parasitica) on Eggs of Rainbow Trout (Oncorhynchus mykiss)

This study was designed to prevent fungal disease (Saprolegnia parasitica) that occurs on rainbow trout eggs (Oncorhynchus mykiss) by using wastes of onion (Allium cepa) and garlic (Allium sativum) plants. For this purpose, fertilized rainbow trout eggs were exposed to garlic skin, garlic stem and onion skin aqueous methanolic extracts by bathing in concentrations of 0.4, 0.8, 1.6 and 3.2 g/L, whereas the control group was left untreated. The larvae in all groups were monitored until they become free-swimming larvae, the number of eggs died due to fungus was recorded, and the data obtained from experimental groups and control group were compared. As a result, it was determined that onion skin had no effect on the number of eggs that died due to fungus (p > .05), while garlic skin and garlic stem extracts significantly reduced the number of fungal infestations without affecting the number of live larvae or the embryological development of the eggs (p < .05). In light of these data, we conclude that garlic skin (0.4, 0.8, and 1.6 g/L) and garlic stem (0.8 and 1.6 g/L) aqueous methanolic extracts are effective at preventing Saprolegnia parasitica infestation on rainbow trout eggs and may be used in aquaculture.     

Carcase characteristics of quail (Coturnix coturnix japonica) slaughtered at different ages

1. The present study was conducted to evaluate the relationship between slaughter ages and carcase characteristics of Japanese quail.

2. Chicks were slaughtered at 5, 6, 7, 8 and 9 weeks of age. Carcase yield, carcase parts weight, weight of liver, heart, gizzard and alimentary tract were obtained. Protein, fat, dry matter and ash contents of meat were analysed for each sex.

3. Body weight and eviscerated weight were affected by age. Age had no significant effect on the weight of liver, alimentary tract, gizzard and heart. Age had first and second order polynomial effects on breast weight and relative leg weight. Meat compositions of leg and breast were significantly affected by age.     

Genetic heterogeneity of bovine viral diarrhoea virus (BVDV) isolates from Turkey: identification of a new subgroup in BVDV-1

Genetic heterogeneity of Turkish ruminant pestiviruses was investigated by phylogenetic analysis of complete N(pro) encoding nucleotide sequences. A total of 30 virus isolates obtained from 15 provinces around the country between 1997 and 2005 were included in the phylogenetic analysis. Virus isolates mostly originated from cattle with one isolate from sheep. The bovine isolates all belonged to BVDV-1, the sheep isolate to BVDV-2. Fifteen isolates formed a new subgroup within BVDV-1, tentatively named BVDV-1l. The remaining bovine isolates were typed as BVDV-1a (n=4), BVDV-1b (n=4), BVDV-1d (n=3), BVDV-1f (n=2) and BVDV-1h (n=1). The isolates allocated to BVDV-1l originated from various geographical regions in different years. There was no correlation between genetic grouping and locations where isolates were obtained. Viruses originating from one farm in most cases belonged to the same subgroup (n=5). This study indicates that the newly detected subgroup BVDV-1l is predominant and widespread in Turkey. Moreover, an ovine virus isolate was identified as the first member of BVDV-2 reported in Turkey. A serological survey using samples from western Turkey indicated that BVDV-2 is also present in cattle.     

A survey on Tetracapsuloides bryosalmonae infections in Slovene fresh waters

Slovenia has no history of health problems related to proliferative kidney disease (PKD) either in farmed or in wild fish. However, due to the past molecular evidence for the presence of Tetracapsuloides bryosalmonae DNA in tissues of some fish from open waters, a survey was conducted on wild salmonids that were primarily sampled for other purposes. In winter 2010–2011, specimens from a total of 244 rainbow trout, Oncorhynchus mykiss (Walbaum), and brown trout, Salmo trutta L., from 30 bodies of fresh water were examined for T. bryosalmonae using a PCR method. The sampled fish showed no clinical signs or gross pathological lesions characteristic of PKD. Nineteen (7.8%) fish from seven (23.3%) fresh waters were positive for T. bryosalmonae. The identity of PCR amplicons was confirmed by sequencing. With one exception, all the positive fish were found in waters from the regions where the average yearly temperatures and the environmental pollution are higher. This preliminary countrywide survey provided the first insight into the situation regarding T. bryosalmonae infection of wild salmonids in Slovenia.