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91.
本文以青鳞鱼鱼肉为原料,采有冷水法和酶水解法制备提取物并对其在加热过程中的性质进行了研究。结果表明:①青鳞鱼鱼肉蛋白含量为68.3-71%且水溶性蛋白与水不溶性蛋白之比约为2:9;②酶水解法提取物非蛋白氮约为其总氮的50%.而冷水法提取物则为41.5%;③酶水解法提取物在50℃、60℃、70℃、80℃和90℃的温度条件下加热2h后.颜色明显加深,同时产生大量的呈香物质。但常规成分基本无变化;而冷水法提取物在同样的温度条件下,其颜色、滋味等感官特性变化不明显,但常规成分变化却明显;④提取物水溶性蛋白的变性温度为60—70℃,70—90℃时提取物总氮大部分是非蛋白氮。青鳞鱼提取物在加热过程中的这些性质为其加工和利用提供了重要的理论依据。 相似文献
92.
Spotted scat Scatophagus argus exhibits a typical sexual growth dimorphism in which the females grow faster than the males. Growth hormone (GH) is best known as an anterior pituitary hormone fundamental in regulating growth. To clarify the roles in sexual growth dimorphism in the spotted scat, gh cDNA was isolated from the pituitary. A phylogenetic tree was constructed based on the GH amino acid sequences of spotted scat and other vertebrates, and the resulting topology clearly reflects the taxonomic relationship of the Perciformes species selected. Alignment of GH amino acid sequences displayed very high similarity between the spotted scat and the other Perciformes species. The qRT-PCR analysis demonstrated that females exhibited higher pituitary gh mRNA levels than males at 6, 18, and 30 months (P < 0.05), which was 1.84, 4.61 and 6.34 times greater, respectively. In addition, immature males (6 months) exhibited higher pituitary gh mRNA levels than mature males (18 and 30 months). These results imply that the sexual growth dimorphism may be ascribable to the gh levels in pituitary in spotted scat. 相似文献
93.
The Chinese giant salamander (Andrias davidianus) as food and medicinal product has been an important aquaculture object in China. Study of gene function in the Chinese giant salamander requires accurate normalization though the use of appropriate reference genes. In this study, the expression levels of three candidate reference genes including β‐actin, GAPDH and cytb of different tissues, different developmental stages and different challenges in Chinese giant salamander were evaluated by qPCR. The stabilities of these three reference genes were analysed by geNorm, NormFinder and BestKeeper software. The results showed that the expression of GAPDH was more stable than that of β‐actin and cytb in four tissues and at two developmental stages of Chinese giant salamander. Compared with GAPDH and cytb, β‐actin was the most stable in spleen of Chinese giant salamander treated with LPS or GSIV. Therefore, the result showed that GAPDH was the suitable reference gene in different tissues and at different developmental stages of Chinese giant salamander. The β‐actin could be used as a reference gene in spleen of Chinese giant salamander challenged with LPS and GSIV. This study provides convincing information for the GAPDH and β‐actin as suitable reference gene in Chinese giant salamander of different tissues, different developmental stages and different challenges respectively. 相似文献
94.
Preliminary study to evaluate the effects of dietary bile acids on growth performance and lipid metabolism of juvenile genetically improved farmed tilapia (Oreochromis niloticus) fed plant ingredient‐based diets 下载免费PDF全文
A 9‐week feeding experiment was conducted to investigate the effects of dietary bile acids (BAs) on juvenile genetically improved farmed tilapia (GIFT) (Oreochromis niloticus) based on the evaluations of growth performance and parameters relevant to lipid metabolism. Each of five vegetable protein‐based diets containing BAs at a level of 0, 0.05, 0.15, 0.45 or 1.35 g/kg diet was fed to three replicates with 40 fish (8.2 g per fish). The results showed that weight gain (WG) increased significantly with the increase in BAs from 0 to 0.15 g/kg diet and then decreased significantly at a higher BA supplementation. Dietary BAs significantly reduced the crude lipid content in the whole body, muscle and liver tissue of GIFT. Fish fed diet with 1.35 g BAs/kg diet developed serious nuclear migration and vacuolization in hepatocytes. Gall bladder appeared to contain white solid and has fragile capsules. Dietary BA supplementation had significant effects on serum biochemical indices and activities of lipid metabolism enzymes in liver and intestine. In conclusion, dietary bile acid supplementation (0.15 g/kg) can facilitate the lipid metabolism and therefore promote the growth of tilapia. However, overdosed dietary BAs induced gallstone development, disrupted lipid metabolism and depressed the growth performances of GIFT. 相似文献
95.
小瓜虫病是淡水鱼类和观赏鱼类的一种常见寄生虫病。鱼感染小瓜虫后,在血清和体表黏液中产生抗体,这种抗体主要是鱼受到小瓜虫体细胞表面膜蛋白的刺激,通过血液和体表黏液中淋巴细胞产生出来的,以阻止小瓜虫的运动并杀死虫体。目前还没有正式用于生产的小瓜虫疫苗,但实验室疫苗已研制成功,包括经冷冻和福尔马林处理后的死疫苗、小瓜虫活疫茁以及小瓜虫基因疫苗等。综述了,多年来小瓜虫病的免疫研究概况、鱼类对小瓜虫免疫反应类型、反应机制以及抗小瓜虫疫苗等。 相似文献
96.
珠江斑鳠年龄和生长的研究↑(*) 总被引:4,自引:0,他引:4
观察珠江斑鳠的胸鳍和鳃盖骨,研究其年龄特征、渔获物年龄组成和体长、体重组成,并对其体长与鳃盖骨长、体长与体重的关系,以及生长规律进行了分析。其年轮形成时间为12 月~翌年3 月;体长与鳃盖骨长的关系式为L= 34 .76 R+ 0 .453 3 ,体长与体重的关系式为 W= 0 .032 L3 .187 2 。生长Von Bertalanffy方程分别为Lt = 156 .75〔1 - e- 0 .089(t- 0 .016 8)〕和Wt = 31 193 .17〔1 - e- 0 .089( t- 0 .016 8)〕3 .187 2 。还讨论了年轮形成原因和生长特性,并对合理利用自然资源和生产养殖提出建议。 相似文献
97.
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99.
采用PAC-IMBR组合工艺处理屠宰废水,考察了该工艺对COD、BOD、NH3-N和浊度的去除效果。实验结果表明:组合工艺对COD、BOD和NH3-N的去除效果较好,去除率分别为95.56%、95.9%和91.1%,出水COD约为40 mg/L,NH3-N基本在10 mg/L以下,分别达到了《污水综合排放标准》(GB 8978-1996)的二级和一级标准;出水浊度<1 NTU,出水水质稳定。 相似文献
100.