Flow alteration and thermal pollution depress modelled growth rates of an iconic riverine fish,the Murray cod Maccullochella peelii

The serial discontinuity concept (SDC) proposes that hypolimnetic‐releasing impoundments cause major disruptions to the naturally occurring physical, chemical and biological gradients of rivers but that this impact diminishes with distance downstream. Such a gradient in discharge, flow velocity and temperature regime occurs below a large hypolimnetic‐releasing impoundment, the Hume Dam, on the River Murray in south‐eastern Australia. To examine the effects of this disturbance gradient on a warm‐water large‐bodied freshwater fish, the Murray cod (Maccullochella peelii), a bioenergetics model was developed and calibrated to explore energy expended under differing water velocities and temperature regimes. Model simulations predicted negative growth of juveniles directly downstream of the impoundment, due largely to the energetic costs associated with active and, to a lesser extent, standard metabolism outweighing the achievable energetic gains through food consumption. As flow velocity and temperature regimes became more favourable downstream, so did the simulated growth of the species. It was not until +239 km downstream of the impoundment that the model predicted that flow velocity and temperature regimes were suitable for greater weight gains. The modelled growth responses of juvenile Murray cod are consistent with the predictions of the SDC, emphasising that changes in the bioenergetics of individuals are likely to be reflected in reduced growth rates under the changed flow velocity and temperature regimes imposed by disturbance gradients. This research represents a valuable step in the biological understanding of Murray cod within variable riverine environments and emphasises the urgency required to mitigate impacts associated with hypolimnetic impoundments.     

Evaluation of Heterotrophic Algae Meal as a Diet Ingredient for Channel Catfish,Ictalurus punctatus

Most microalgae evaluated in aquaculture diets have been produced autotrophically. In order to produce a cost‐efficient biomass at greater magnitudes for biofuel feedstock, heterotrophic production may be warranted. However, the chemical/nutritional attributes of these microalgae could differ from those grown autotrophically. An 8‐wk feeding trial was conducted to evaluate Chlorella spp. algae meal (AM) that had been cultured heterotrophically. The oil (lipid) was extracted to simulate biofuel production. Juvenile channel catfish, Ictalurus punctatus (5.7 ± 1.4 g; 8.9 ± 0.8 cm), were stocked at 10 fish/tank into fifteen 37.7‐L aquaria in a closed recirculating system and fed one of the five experimental diets to apparent satiation twice daily. Diets contained either 0 (control, CTL), 10, 20, or 40% AM and an additional diet containing 40% AM was supplemented with 2% lysine (40% AM+LYS). After 8 wk, there were no statistically significant differences in terms of survival, dressout percentages, whole‐body proximate composition, or fatty acid composition of the fillets among fish fed the diets containing varying levels of AM without added lysine. Feed consumption and weight gain for fish fed the 10, 40, and 40% AM+LYS diets were significantly greater than those fed the CTL diet. Feed conversion ratio was significantly lower for fish fed the 40% AM+LYS diet compared to those fed all other diets, which did not differ significantly from each other. These data indicate that channel catfish readily accept and can efficiently utilize heterotrophically produced AM at levels up to at least 40% of the total diet and that AM may enhance diet palatability.     

Population‐level effects of egg predation on a native planktivore in a large freshwater lake

Using a 37‐year recruitment time series, we uncovered a field pattern revealing a strong, inverse relationship between bloater Coregonus hoyi recruitment success and slimy sculpin Cottus cognatus biomass in Lake Michigan (United States), one of the largest freshwater lakes of the world. Given that slimy sculpins (and deepwater sculpin Myoxocephalus thompsonii) are known egg predators that spatiotemporally overlap with incubating bloater eggs, we used recently published data on sculpin diets and daily ration to model annual bloater egg consumption by sculpins for the 1973–2010 year‐classes. Although several strong year‐classes were produced in the late 1980s when the proportion of eggs consumed by slimy sculpins was extremely low (i.e., <0.001) and several weak year‐classes were produced when the proportion of bloater eggs consumed was at its highest (i.e., >0.10–1.0), egg predation failed to explain why recruitment was weak for the 1995–2005 year‐classes when the proportion consumed was also low (i.e., <0.02). We concluded that egg predation by slimy and deepwater sculpins could have limited bloater recruitment in some years, but that some undetermined factor was more important in many other years. Given that slimy sculpin densities are influenced by piscivorous lake trout Salvelinus namaycush, the restoration of which in Lake Michigan has lagged behind those in lakes Superior and Huron, our study highlights the importance of an ecosystem perspective when considering population dynamics of fishes.     

Effect of Caffeine Added to the Activating Solution on Sperm Motility of Fresh and Thawed Semen of Pacu,Piaractus mesopotamicus,and Curimba,Prochilodus lineatus

The aim was to evaluate the effect of different concentrations of caffeine added in activating solution over sperm motility in fresh and thawed semen of pacu, Piaractus mesopotamicus, and curimba, Prochilodus lineatus. The activating solutions were prepared with sodium bicarbonate solution of 0.76% (NaHCO₃) and caffeine was added at concentrations of 2.5, 5.0, 10.0, and 20.0 mM. As control, a solution of NaHCO₃ 0.76 without caffeine was used. Eight males of pacu and 20 males of curimba were used. Aliquots of 200 μL of semen were diluted in 800 μL extender solution (DMSO 10% and BTS 5%), placed in 0.5 mL straws and cryopreserved for 7 d in a liquid nitrogen tank. There was a linear increase in sperm motility for fresh semen of pacu, and for curimba fresh and thawed semen (P < 0.05), due to the increase in the concentration of caffeine. There was a quadratic response for duration of motility for thawed semen of pacu and for fresh semen of curimba (P < 0.05), respectively. These results indicate that addition of caffeine in the activator solution can improve sperm motility parameters, however, is dependent on the species and concentration used.     

Transient bilateral blindness associated with presumptive idiopathic pachymeningitis in a 22-year-old Irish Sport Horse

A 22-year-old Irish Sport Horse presented following a period of bilateral blindness with subsequent spontaneous resolution. Brain magnetic resonance imaging (MRI) demonstrated features consistent with pachymeningitis. Cerebrospinal fluid (CSF) analysis revealed increased numbers of basophils, suggestive of an immunologic or neoplastic aetiology. It was suspected that the enlarged meninges led to compression of the optic nerves in the optic canals, causing bilateral blindness. Initial spontaneous remission, as seen in this case, has been reported in human patients with this condition. Pachymeningitis should be considered as a possible cause of cranial nerve deficits, including bilateral and unilateral blindness, in horses.     

Major ascaroside pheromone component asc-C5 influences reproductive plasticity among isolates of the invasive species pinewood nematode

Pheromones are communication chemicals and regulatory signals used by animals and represent unique tools for organisms to mediate behaviors and make “decisions” to maximize their fitness. Phenotypic plasticity refers to the innate capacity of a species to tolerate a greater breadth of environmental conditions across which it adapts to improve its survival, reproduction, and fitness. The pinewood nematode, Bursaphelenchus xylophilus, an invasive nematode species, was accidentally introduced from North America into Japan, China, and Europe; however, few studies have investigated its pheromones and phenotypic plasticity as a natural model. Here, we demonstrated a novel phenomenon, in which nematodes under the condition of pheromone presence triggered increased reproduction in invasive strains (JP1, JP2, CN1, CN2, EU1, and EU2), while it simultaneously decreased reproduction in native strains (US1 and US2). The bidirectional effect on fecundity, mediated by presence/absence of pheromones, is henceforth termed pheromone-regulative reproductive plasticity (PRRP). We further found that synthetic ascaroside asc-C5 (ascr#9), the major pheromone component, plays a leading role in PRRP and identified 2 candidate receptor genes, Bxydaf-38 and Bxysrd-10, involved in perceiving asc-C5. These results suggest that plasticity of reproductive responses to pheromones in pinewood nematode may increase its fitness in novel environments following introduction. This opens up a new perspective for invasion biology and presents a novel strategy of invasion, suggesting that pheromones, in addition to their traditional roles in chemical signaling, can influence the reproductive phenotype among native and invasive isolates. In addition, this novel mechanism could broadly explain, through comparative studies of native and invasive populations of animals, a potential underlying factor behind of the success of other biological invasions.     

Death of a neonatal lamb due to Clostridium perfringens type B in New Zealand

ABSTRACT

Case history and clinical findings: A flock of 20 sheep was kept within three paddocks on a single property. None of the animals in the flock had been vaccinated against any disease for at least three years. Abdominal bloating and haemorrhagic diarrhoea were observed in Lamb 1 at 24 hours-of-age. The lamb subsequently died within an hour of the onset of clinical signs. Lamb 2 was 3-days-old when observed to be recumbent with opisthotonus. The lamb was treated with dextrose, vitamins B1 and B12, and penicillin G, but died 4 hours later.

Pathological findings: Examination of Lamb 1 revealed markedly increased gas within the peritoneum and within dilated loops of intestine. The intestines were dark red and contained large quantities of haemorrhagic fluid. Histology of the intestines revealed peracute mucosal necrosis with minimal accompanying inflammation. The intestinal lumen contained cell debris, haemorrhage, and myriad large Gram-positive bacilli. The intestines of Lamb 2 did not appear bloated or reddened. However, multiple fibrin clots were visible within the pericardial sac. Histopathological examination revealed small foci of necrosis within the mucosa of the distal intestine. The necrotic foci were often associated with large numbers of large Gram-positive bacilli.

Immunohistochemsitry and molecular biology: Intestinal samples from Lamb 1 were processed for Clostridium perfringens immunohistochemistry, which revealed large numbers of intralesional, positively immunostained rods. Fragments corresponding to the expected sizes for genes encoding alpha, beta, and epsilon C. perfringens typing toxins were amplified by PCR from DNA extracted from formalin-fixed sections of intestine.

Diagnosis: Lamb dysentery due to C. perfringens type B.

Clinical relevance: C. perfringens bacteria have a worldwide distribution, but disease due to C. perfringens type B has only been diagnosed in a small number of countries and has never been reported in New Zealand or Australia. C. perfringens type B produce both beta toxin and epsilon toxins, therefore both haemorrhagic enteritis and systemic vascular damage can develop. As many animals are exposed to C. perfringens without developing disease, there must be additional unknown factors that resulted in disease in these particular sheep. Vaccines that specifically protect against C. perfringens type B are available and may be recommended for use in smaller non-commercial flocks, as in the present case.     

Xylanase and xylo- oligosaccharide prebiotic improve the growth performance and concentration of potentially prebiotic oligosaccharides in the ileum of broiler chickens

ABSTRACT

1. The objective of this study was to investigate the effect of supplementing broiler diets with xylanase or xylo- oligosaccharide (XOS) on growth performance, the concentration of non-starch polysaccharide (NSP) hydrolysis products in the ileum and concentration of short chain fatty acids (SCFA) in the caeca of broiler chickens.

2. In total, 500 male Ross 308 broilers were used in this 29-day (d) study. The treatments were organised into a 2 × 2 plus 1 factorial arrangement consisting of two additives (xylanase or XOS) at two levels (low or high) plus a control treatment with no additives. This gave five treatments with 100 birds in each treatment group. The diets were slightly deficient in protein by 20 g/kg and energy by 1 MJ/kg.

3. On d 14 and 28, two birds per pen were euthanised, the caeca content collected and analysed for short chain fatty acid (SCFA) concentration. On d 29, six birds per pen were euthanised and ileal digesta were collected and analysed for the concentration of NSP fractions.

4. On d 14, caecal acetic acid, iso-butyric acid, iso-valeric acid, n-valeric acid and total SCFA concentrations were significantly greater (P ≤ 0.05) when diets were supplemented with XOS compared with xylanase.

5. Ileal concentration of arabinose, galactose and glucuronic acid (GlucA2) were significantly greater (P ≤ 0.05) in the insoluble NSP fraction when diets were supplemented with a high level of xylanase, compared with the control treatment. Ileal concentration of fructose was significantly greater (P ≤ 0.05) in the water soluble NSP when a high level of xylanase or low level of XOS were included in the diet compared with the control.

6. It was concluded that xylanase and XOS had similar effects on NSP concentration and SCFA in the caeca, although there was little effect on performance. This observation demonstrated further benefits of xylanase supplementation in wheat-based broiler diets beyond digesta viscosity reduction and the release of extra nutrients.     

Bilateral intracorporeally sutured inguinal herniorrhaphy using 3-dimensional laparoscopy in a dog

A 7-month-old, intact male, mixed breed dog with bilateral inguinal hernias underwent general anesthesia for laparoscopic bilateral inguinal herniorrhaphy via a 3-port approach. A 3-dimensional laparoscopic system was used to perform the procedure immediately following prescrotal open castration. Intracorporeal suturing with polypropylene was performed, and 2 cruciate sutures were placed to close each inguinal ring. The caudal aspect of each inguinal ring was left slightly open so as not to disrupt the passage or patency of vessels and nerves. No intra- or post-operative complications occurred. One year after surgery, the dog has no evidence of recurrence of the inguinal hernias.Key clinical message:This case report demonstrates a novel minimally invasive approach to inguinal herniorrhaphy in a dog with no reported complications and a good long-term outcome. Intracorporeally sutured inguinal herniorrhaphy is feasible in dogs with good results, although additional cases are needed to gain experience with this technique in dogs with varying presentations of inguinal hernias.     

Time‐lapse monitoring of soil water content using electromagnetic conductivity imaging

The volumetric soil water content (θ) is fundamental to agriculture because its spatiotemporal variation in soil affects the growth of plants. Unfortunately, the universally accepted thermogravimetric method for estimating volumetric soil water content is very labour intensive and time‐consuming for use in field‐scale monitoring. Electromagnetic (EM) induction instruments have proven to be useful in mapping the spatiotemporal variation of θ. However, depth‐specific variation in θ, which is important for irrigation management, has been little explored. The objective of this study was to develop a relationship between θ and estimates of true electrical conductivity (σ) and to use this relationship to develop time‐lapse images of soil θ beneath a centre‐pivot irrigated alfalfa (Medicago sativa L.) crop in San Jacinto, California, USA. We first measured the bulk apparent electrical conductivity (EC_a – mS/m) using a DUALEM‐421 over a period of 12 days after an irrigation event (i.e. days 1, 2, 3, 4, 6, 8 and 12). We used EM4Soil to generate EM conductivity images (EMCIs). We used a physical model to estimate θ from σ, accounting for soil tortuosity and pore water salinity, with a cross‐validation RMSE of 0.04 cm³/cm³. Testing the scenario where no soil information is available, we used a three‐parameter exponential model to relate θ to σ and then to map θ along the transect on different days. The results allowed us to monitor the spatiotemporal variations of θ across the surveyed area, over the 12‐day period. In this regard, we were able to map the soil close to field capacity (0.27 cm³/cm³) and approaching permanent wilting point (0.03 cm³/cm³). The time‐lapse θ monitoring approach, developed using EMCI, has implications for soil and water use and management and will potentially allow farmers and consultants to identify inefficiencies in water application rates and use. It can also be used as a research tool to potentially assist precision irrigation practices and to test the efficacy of different methods of irrigation in terms of water delivery and efficiency in water use in near real time.