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31.
Meighan Daly Kelly L Diegel Scott D Fitzgerald Angie Schooley Dale E Berry John B Kaneene 《Journal of veterinary diagnostic investigation》2006,18(4):401-404
The state of Michigan has recognized the presence of Mycobacterium bovis in its free-ranging white-tailed deer population since 1994. This endemic infection is primarily located in a 12-county area in the northeastern lower peninsula of Michigan. A statewide surveillance and eradication program of the disease has been in effect since 1994. Worldwide, Mycobacterium tuberculosis complex organisms have a known predilection toward development of antimicrobial resistance. The objective of this study was to investigate the antimicrobial susceptibility of M. bovis isolates from white-tailed deer in Michigan and detect any changes in susceptibility over time. M. bovis isolates from 2 fall hunting seasons (1999 and 2004) were used in this study. The fall season of 2004 marked the first documented case of direct transmission of M. bovis from a wild deer to a human in Michigan. Since M. bovis is a zoonotic disease, knowledge of susceptibility can expedite treatment options in humans. M. bovis isolates were obtained from 58 deer, 4 coyotes, 3 cattle, 2 raccoons, and 1 human case from the 2 years combined. Methods of susceptibility testing included 1% proportion agar plates and Bactec radiometric broth testing. M. bovis was found to be uniformly resistant to the antibiotic pyrazinamide; this resistance is common to all M. bovis isolates. No other antimicrobial resistance was found in any of the tested M. bovis isolates, which may be, in part, attributed to the lack of any significant treatment pressure in wildlife. 相似文献
32.
David J Everest Susan Waterhouse Tina Kelly Elena Velo-Rego Maurice J Sauer 《Journal of veterinary diagnostic investigation》2007,19(5):552-557
Management of prion diseases in livestock would benefit greatly from availability of a validated blood test. A promising immunocapillary electrophoresis technique (also known as capillary electrophoresis fluoroimmunoassay) to detect abnormal prion protein in blood from live sheep is evaluated here. Capillary electrophoresis fluoroimmunoassay was applied to analysis of extracted blood from scrapie-exposed sheep (n = 87; 347 samples) at various stages of incubation, and to control sheep (n = 194; 489 samples). Overall, test values for the control and test populations were not significantly different, and a similar proportion of control (7%) and test (10%) sheep were classified as positive. Over 2-3 month intervals from birth until clinical disease, test specificity and sensitivity ranged from 66.7% to 100% and 0% to 66.7%, respectively, indicating poor diagnostic performance at all stages of pathogenesis. In routine application, in its present form, the capillary electrophoresis fluoroimmunoassay procedure proved to be insufficiently robust for use as a blood test for scrapie diagnosis. 相似文献
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Nickolee Zollinger Roger Kjelgren Teresa Cerny-Koenig Kelly Kopp Rich Koenig 《Scientia Horticulturae》2006
Although low water use landscaping is becoming common in arid regions, little is known about drought tolerance and drought responses of many ornamental plants, especially herbaceous perennials. Drought responses were assessed for six herbaceous ornamental landscape perennials in a 38 l pot-in-pot system in northern Utah over a 2-year period. The first year was an establishment period. During the second year, drought responses were evaluated for established Echinacea purpurea (L.) Moench, Gaillardia aristata Pursh, Lavandula angustifolia P. Mill., Leucanthemum × superbum (J.W. Ingram) Berg. ex Kent, ‘Alaska’, Penstemon barbatus Roth var. praecox nanus rondo, and Penstemon × mexicali Mitch. ‘Red Rocks’. Plants were irrigated at frequencies of 1 (control), 2, or 4 weeks between June and September, simulating well-watered conditions, moderate drought, or severe drought. Osmotic potential (Ψs), gas exchange, visual quality, leaf area, and dry weight were assessed. In a confined root zone, P. barbatus showed the greatest tolerance to all levels of drought, avoiding desiccation by increasing root:shoot ratio and decreasing stomatal conductance as water became limiting. L. angustifolia and P. × mexicali showed tolerance to moderate drought conditions, but died after exposure to the first episode of severe drought. Neither G. aristata nor L. superbum were able to regulate shoot water loss effectively. Instead, both species displayed drought avoidance mechanisms, dying back when water was limiting and showing new growth after they were watered. Compared to control plants, G. aristata shoot dry weight was reduced by 50% and 84%, and L. superbum shoot dry weight was reduced by 47% and 99% for the 2- and 4-week irrigation intervals, respectively. Root dry weights were affected similarly for both species. E. purpurea exhibited poor visual quality at all irrigation intervals, in particular wilting severely in both drought treatments, but regaining turgor when watered again. P. barbatus is recommended for ornamental landscapes that receive little or no supplemental irrigation, while E. purpurea is not recommended for low water landscapes because of low visual quality under even mild drought. 相似文献
36.
A Syndrome Resembling Idiopathic Noncirrhotic Portal Hypertension in 4 Young Doberman Pinschers 总被引:1,自引:0,他引:1
JoAnn DeMarco Sharon A. Center Nathan Dykes Amy E. Yeager Bruce Kornreich Ed Gschrey Kelly A. Credille Magali Guffroy Fabio del Piero Beth A. Valentine 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(3):147-156
We describe 4 young male Doberman Pinschers (3 littermates and 1 unrelated dog) with a syndrome resembling idiopathic or noncirrhotic portal hypertension of humans. Each dog was evaluated for a hepatopathy resulting in portal hypertension, development of portosystemic collateral vessels, and hepatic encephalopathy. These dogs differ from previous reports of young dogs with hepatic insufficiency associated with portal hypertension and acquired portal systemic shunting by their lack of intrahepatic arteriovenous fistulae, portal vein atresia, or intrahepatic fibrosis. Clinicopathologic features included erythrocyte microcytosis, normal to mildly increased liver enzyme activities, increased concentrations of serum bile acids, reduced plasma indocyanine green clearance, and normal total bilirubin concentration. Abdominal ultrasonography disclosed a small liver and portosystemic collateral vessels. Radiographic imaging studies confirmed hepatofugal portal circulation and discounted hepatic arteriovenous fistulae. Histopathologic features in liver tissue from each dog were similar and consistent in all sections examined. Common findings included increased cross-sectional views of hepatic arterioles; hepatic lobular atrophy; scanty increase in connective tissue around some large portal triads; and absence of inflammation, disturbed lobular architecture, bile duct proliferation, or intrahepatic cholestasis. 相似文献
37.
Robert A. Spotts Kelly M. Wallis Maryna Serdani Daniel T. O’Gorman Peter L. Sholberg 《Postharvest Biology and Technology》2009,51(2):227-231
The objectives of this study were to develop primers and a real time PCR protocol for the postharvest biocontrol yeast Cystofilobasidium infirmominiatum (Cim). The application of this technology was developed to quantify Cim on the surfaces of apple, two pear cultivars, and sweet cherry fruit treated over a range of concentrations. Statistically significant relationships were observed between Cim DNA on fruit surfaces, expressed as μg/m2, and CFU/L of dip suspensions for apple, pear, and sweet cherry. In addition, the relationship for each fruit was significantly different from the other three fruits. Threshold values of concentrations of Cim DNA on the fruit surface were calculated based on regression equations and a dose of 2.0 × 1011 CFU/L of dip suspension, the dose for optimum decay control, and were 4.8, 7.0, 16.5, and 25.2 μg/m2 for Bosc pear, Lapins sweet cherry, d’Anjou pear, and Golden Delicious apple, respectively. Monitoring Cim DNA concentration on fruit surfaces will assure that Cim is being properly applied to fruit and that a sufficient number of cells are present for optimum decay control. 相似文献
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Veterinary Research Communications - 相似文献
40.
Cryptosporidiosis in the domestic cat 总被引:2,自引:0,他引:2
M Bennett D Baxby N Blundell C J Gaskell C A Hart D F Kelly 《The Veterinary record》1985,116(3):73-74