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51.
M. Scortichini D. D'Ascenzo Daniela Di Silvestro 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):625-627
In spring 1996, extensive leaf necrosis and twig dieback were observed on young sweet persimmon (Dyospiros kaki L.) trees, cultivars O'Gosho, Hachija, Mercatelli and Kaki-tipo planted in the Abruzzo region (central Italy). Many trees were killed. When the dieback reached the trunk, in many cases, new vegetation was noticed above the graft point. The cultivar Jiro-C was not affected by the disease. During 1997, no symptoms were observed on any plant. The orchard was planted in a clay soil with a very low content of organic matter. Biochemical, nutritional and pathogenicity tests indicated Pseudomonas syringae pv. syringae van Hall as the causal agent of the disease. This is the first report of this bacterium as a pathogen of sweet persimmon in Europe. 相似文献
52.
Pietro Rocculi Emiliano Cocci Santina Romani Giampiero Sacchetti Marco Dalla Rosa 《Postharvest Biology and Technology》2009,51(3):371-377
The effects of both 1-MCP treatment of pineapples and packaging of their fresh-cut products with an alternative modified atmosphere (MA: 86.13 kPa N2O, 10.13 kPa O2 and 5.07 kPa CO2) on physiological and quality changes of these minimally processed products were investigated. Fresh-cut fruit treated or not with 1-MCP were packed in Air or in MA and were stored at 4 °C for 10 d. The following parameters were monitored during storage: ripening index; O2, CO2 and C2H4 in the package headspace; firmness and colour. Microbial spoilage of MP pineapple samples was also investigated and a mathematical model based on the Zwietering modified Gompertz equation was used to obtain growth parameters of mesophilic bacteria, yeasts and moulds.The results showed that 1-MCP treatment and MAP in a N2O enriched atmosphere had a positive combined effect on the inhibition of respiration and ethylene production of fresh-cut pineapple and on its softening delay, confirming previous findings about 1-MCP and N2O preservative effects on fresh-cut fruit quality. This combined effect was not extended to the ripening index and colour maintenance, as MAP at 86.13 kPa of N2O did not add any benefit to that of the 1-MCP treatment. From a microbiological point of view, N2O MAP extended the shelf-life of the products of 3–4 d by increasing the lag phase of microbial growth. 相似文献
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The methanol and ethyl acetate extracts of the aerial parts of Cirsium tenoreanum, have been evaluated for their phytochemical constituents and antibacterial and cytotoxic properties. Three flavonoids, apigenin, quercetin-3-O-galactoside and kaempferol-3-O-ramnoside, were isolated. The ethyl acetate extract showed a good antiproliferative activity. 相似文献
56.
Summary The effects of heating cork in water vapour at 100°C and 300°C are studied and compared with those produced by heating in
air at the same temperatures. Dimensional and mass changes were measured and radial compression curves were obtained following
various treatments. The 300°C treatments originate a straightening of the originally corrugated cell walls, while the cell
wall material undergoes thermal degradation. Larger swellings and larger mass changes are observed in the treatments in water
vapour as compared to those in air. The 100°C treatments do not induce degradation and produce reversible changes in technological
properties which are simply due to differences in the water content. 相似文献
57.
Canine cutaneous melanocytic tumours: significance of β‐catenin and survivin immunohistochemical expression 下载免费PDF全文
58.
Rosa Gagliardi Silvia Llambí M. Victoria Arruga 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(3):273-280
The fields of pharmacogenetics and pharmacogenomics have become increasingly promising regarding the clinical application of genetic data to aid in prevention of adverse reactions. Specific screening tests can predict which animals express modified proteins or genetic sequences responsible for adverse effects associated with a drug. Among the genetic variations that have been investigated in dogs, the multidrug resistance gene (MDR) is the best studied. However, other genes such as CYP1A2 and CYP2B11 control the protein syntheses involved in the metabolism of many drugs. In the present study, the MDR-1, CYP1A2 and CYP2B11 genes were examined to identify SNP polymorphisms associated with these genes in the following four canine breeds: Uruguayan Cimarron, Border Collie, Labrador Retriever and German Shepherd. The results revealed that several SNPs of the CYP1A2 and CYP2B11 genes are potential targets for drug sensitivity investigations. 相似文献
59.
A sensitive and specific method is described for the simultaneous determination of oxytetracycline, tetracycline (TC), and chlortetracycline residues in edible swine tissues, by combining liquid chromatography with spectrofluorometric and mass spectrometry detection. The procedure involved a preliminary extraction with EDTA-McIlvaine buffer acidified at pH 4.0, followed by solid-phase extraction cleanup using a polymeric sorbent. The liquid chromatography analysis was performed with spectrofluorometric detection after postcolumn derivatization with magnesium ions. The limits of quantification were 50 microg/kg for muscle and 100 microg/kg for kidney tissues. The recovery values were greater than 77.8% for muscle and 65.1% for kidney. The method has been successfully used for the quantification of tetracyclines in swine tissues samples. The selective liquid chromatography mass spectrometric analysis for confirmation of oxytetracycline in one positive swine muscle sample was made by atmospheric pressure chemical ionization (APCI). The APCI mass spectra of the TCs gave the protonated molecular ion and two typical fragment ions, required for their confirmation in single ion monitoring scan mode in animal tissues. 相似文献
60.
Penndorf I Biedermann D Maurer SV Henle T 《Journal of agricultural and food chemistry》2007,55(3):723-727
To obtain information about the extent of the early Maillard reaction between the N-termini of peptides and lactose, alpha-N-(2-furoylmethyl) amino acids (FMAAs) were quantified together with epsilon-N-(2-furoylmethyl)lysine (furosine) in acid hydrolyzates of hypoallergenic infant formulas, conventional infant formulas, and human milk samples using RP-HPLC with UV-detection. FMAAs are formed during acid hydrolysis of peptide-bound N-terminal Amadori products (APs), and furosine is formed from the Amadori products of peptide-bound lysine. Unambiguous identification was achieved by means of LC/MS and UV-spectroscopy using independently prepared reference material. The extent of acid-induced conversion of APs to FMAAs was studied by RP-HPLC with chemiluminescent nitrogen detection (CLND). Depending on the corresponding alpha-N-lactulosyl amino acid, between 6.0% and 18.1% of FMAAs were formed during hydrolysis for 23 h at 110 degrees C in 8 N HCl. From epsilon-N-lactulosyllysine, 50% furosine is formed under these conditions. Whereas furosine was detectable in all assayed samples, five different FMAAs, alpha-FM-Lys, alpha-FM-Ala, alpha-FM-Val, alpha-FM-Ile, and alpha-FM-Leu, were exclusively detected in acid hydrolyzates of hypoallergenic infant formulas in amounts ranging from 35 to 396 mumol/100 g protein. Taking the conversion factors into account, modification of N-terminal amino acids in peptides by reducing carbohydrates was between 0.3% and 8.4%. This has to be considered within the discussion concerning the nutritional quality of peptide-containing foods. 相似文献