全文获取类型
收费全文 | 636篇 |
免费 | 41篇 |
专业分类
林业 | 37篇 |
农学 | 16篇 |
134篇 | |
综合类 | 44篇 |
农作物 | 45篇 |
水产渔业 | 63篇 |
畜牧兽医 | 271篇 |
园艺 | 24篇 |
植物保护 | 43篇 |
出版年
2024年 | 2篇 |
2023年 | 11篇 |
2022年 | 20篇 |
2021年 | 44篇 |
2020年 | 31篇 |
2019年 | 41篇 |
2018年 | 29篇 |
2017年 | 23篇 |
2016年 | 30篇 |
2015年 | 21篇 |
2014年 | 30篇 |
2013年 | 34篇 |
2012年 | 51篇 |
2011年 | 59篇 |
2010年 | 30篇 |
2009年 | 28篇 |
2008年 | 41篇 |
2007年 | 37篇 |
2006年 | 40篇 |
2005年 | 24篇 |
2004年 | 23篇 |
2003年 | 9篇 |
2002年 | 10篇 |
2000年 | 4篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
排序方式: 共有677条查询结果,搜索用时 15 毫秒
61.
Celano G Smejkalová D Spaccini R Piccolo A 《Journal of agricultural and food chemistry》2008,56(16):7360-7366
The adsorption of three s-triazines (atrazine, terbutylazine, and simazine) on three different humic acids (HAs), before and after acidic hydrolysis, was studied at four diverse pH values from 3 to 4.5. The Freundlich sorption affinity (K(f)) and intensity (1/n) of s-triazines were related to the chemical and structural composition of HA and used in a multivariate statistical analysis. At low pH, the sorption values for s-triazines were not directly justified by the content of carboxyl groups in original HA, while only an increase of phenolic carbons in hydrolyzed HA supported the increased s-triazine adsorption. The structural composition of both s-triazines and humic samples explained 86% of the significance in multivariate analyses, whereas the role of pH remained hidden in only 14% of the statistical significance. Adsorption of s-triazines was mainly related to carbon content, hydrophobicity, and aromaticity of HA, thereby implying a predominant binding role of weak dispersive forces. Steric properties explained the larger adsorption affinity of atrazine and terbutylazine with respect to simazine. The occurrence of charge-transfer interactions between atrazine and mainly hydrolyzed HA was further suggested by the increased number of free radicals detected in atrazine-HA complexes at different pH values. Charge-transfer interactions were postulated to occur when HA conformations became progressively controlled by aromatic components. This work indicates that humic matter rich in hydrophobic and aromatic constituents is more likely to adsorb s-triazines and reduce their environmental mobility. 相似文献
62.
Elena?Zini Franco?Biasioli Flavia?Gasperi Daniela?Mott Eugenio?Aprea Tilmann?D.?M?rk Andrea?Patocchi Cesare?Gessler Matteo?KomjancEmail author 《Euphytica》2005,145(3):269-279
The availability of genetic linkage maps enables the detection and analysis of QTLs contributing to quality traits of the
genotype. Proton Transfer Reaction Mass Spectrometry (PTR-MS), a relatively novel spectrometric technique, has been applied
to measure the headspace composition of the Volatile Organic Compounds (VOCs) emitted by apple fruit genotypes of the progeny
‘Fiesta’ × ‘Discovery’. Fruit samples were characterised by their PTR-MS spectra normalised to total area. QTL analysis for
all PTR-MS peaks was carried out and 10 genomic regions associated with the peaks at m/z = 28, 43, 57, 61, 103, 115 and 145 were identified (LOD > 2.5). We show that it is possible to find quantitative trait loci
(QTLs) related to PTR-MS characterisation of the headspace composition of single whole apple fruits indicating the presence
of a link between molecular characterisation and PTR-MS data. We provide tentative information on the metabolites related
to the detected QTLs based on available chemical information. A relation between apple skin colour and peaks related to carbonyl
compounds was established.
The two authors contributed equally to this work. 相似文献
63.
Cocchi M Franchini G Manzini D Manfredini M Marchetti A Ulrici A 《Journal of agricultural and food chemistry》2004,52(13):4047-4056
A comparison of different digestion procedures has been carried out for the analysis of metal concentration in samples of vinegars and Aceto Balsamico Tradizionale of Modena (ABTM) coming from an unique barrel set. In particular, classical wet, dry ashing, and closed vessel microwave digestion procedure have been utilized and compared for each investigated species. In a few cases, direct metal determination on ABTM (without treatment procedure) is proposed as possible alternative to sample manipulation. Flame atomic absorption spectrometry was used for the quantification of iron and zinc, while graphite furnace atomic absorption spectrometry was used for all the other elements (i.e., chromium, manganese, cobalt, nickel, copper, cadmium, and lead). The comparison among the different sample treatments was carried out by the use of statistical and chemometric tools. In particular, principal component analysis and ANOVA approaches were used to discriminate between the diverse analytical methods. Furthermore, for all the dissolving techniques, the analytical metal recovery was always evaluated by the application of the recovery function on the same sample matrix. In general, the recoveries were fairly good, ranging from 90 to 103%, except for Cd and Pb with dry ashing, which showed recovery values close to 55% and 67%, respectively. As regards the metals concentration of the investigated samples, the experimental data reveal for some species the presence of concentration slightly over the legal limit fixed for wine and wine vinegar. 相似文献
64.
65.
Zhou M Morgner N Barrera NP Politis A Isaacson SC Matak-Vinković D Murata T Bernal RA Stock D Robinson CV 《Science (New York, N.Y.)》2011,334(6054):380-385
The ability of electrospray to propel large viruses into a mass spectrometer is established and is rationalized by analogy to the atmospheric transmission of the common cold. Much less clear is the fate of membrane-embedded molecular machines in the gas phase. Here we show that rotary adenosine triphosphatases (ATPases)/synthases from Thermus thermophilus and Enterococcus hirae can be maintained intact with membrane and soluble subunit interactions preserved in vacuum. Mass spectra reveal subunit stoichiometries and the identity of tightly bound lipids within the membrane rotors. Moreover, subcomplexes formed in solution and gas phases reveal the regulatory effects of nucleotide binding on both ATP hydrolysis and proton translocation. Consequently, we can link specific lipid and nucleotide binding with distinct regulatory roles. 相似文献
66.
Objective To determine corneal sensitivity in 20 healthy adult alpacas (40 eyes) in order to establish reference values. Design Prospective study. Animals Twenty healthy, adult alpacas. Procedures Corneal sensitivity was determined by the corneal touch threshold (CTT) using a Cochet‐Bonnet esthesiometer. Five different regions of the cornea were evaluated (nasal, ventral, lateral, dorsal, and central). Results Corneal touch threshold values (in mm filament length) obtained from five corneal regions demonstrated varying corneal sensitivities. The central region (34.5 ± 7.1 mm) was the most sensitive, followed by the ventral (29.5 ± 7.2 mm), medial (29.3 ± 7.3 mm), dorsal (25.6 ± 6.3 mm), and lateral (21.8 ± 5.7 mm) regions. All pairwise comparisons of regional differences were statistically significant (P < 0.05), except for the comparison between the ventral and medial regions (P = 0.88). Evaluation of the CCT while controlling for age (2–5 years vs. 6–10 years) demonstrated an increased CTT with an increasing age for the central, ventral, medial and lateral regions. A decrease in the CTT of the dorsal region was noted with increasing age. Conclusion and Clinical Relevance Corneal touch threshold values in 40 eyes of 20 healthy adult alpacas were determined using a Cochet‐Bonnet esthesiometer. This study demonstrated the central corneal region to be most sensitive. Values obtained may serve as reference values in subsequent studies. 相似文献
67.
Emanuela Manno Massimo Vassallo Daniela Varrica Gaetano Dongarrà Sergio Hauser 《Water, air, and soil pollution》2007,178(1-4):179-193
In the study area physical and chemical factors control the composition of surface and groundwaters, which in turn determine the water quality of the “Biviere di Gela” lake. These factors combine to create diverse water types which change their compositional character spatially as rainfall infiltrates the soil zone, moves down a topographically defined flow path, and interacts with bedrock minerals. Low-salinity waters, which represent the initial stage of underground circulation, start dissolving calcium carbonate from the local rocks. The progressive increase in salinity, characterized by substantially higher Ca, SO4, Na and Cl concentrations, suggests that dissolution of CaSO4 and NaCl is an important process during water–rock interactions. The “Biviere di Gela” lake is often separated into two units (Lago Grande and Lago Piccolo). “Lago Grande” water is generally of Na-SO4-Cl-type, whereas “Lago Piccolo” water is of Na-Cl-SO4-type. Their total content of dissolved salts varies with season, the amount of rainfall, and inflow of ground and drainage water. Over time, an increasing trend towards greater salinity and also sudden changes in the relative abundances of Cl and SO4 have been recorded for the “Lago Grande.” The isotope composition of the lake water appears to be affected by inflow of ground and surface waters and also by evaporative loss. The nitrate content of waters from the recharge basin is of particular concern because it contributes to lake eutrophication. The trace element contents do not evidence the presence of any significant metal contamination of lake waters, although a future potential hazard of metals bioaccumulation by the aquatic biota must be taken into consideration. Finally, a water balance for the basin shows that a drop in precipitation of about 20% might be critical for lake survival. 相似文献
68.
69.
Rossi A Rigano D Pergola C Formisano C Basile A Bramanti P Senatore F Sautebin L 《Journal of agricultural and food chemistry》2007,55(13):5053-5061
Feijoa sellowiana Berg. fruits and especially the acetonic extract have been shown to possess biological activities, although the responsible compounds have never been identified. The present study was designed to evaluate the anti-inflammatory activity of an acetonic extract from F. sellowiana Berg. fruits on the nitric oxide (NO) pathway, which plays an important role in inflammation. To this aim the J774 cell line, which expresses inducible nitric oxide synthase (iNOS) following stimulation with lipopolysaccharide (LPS), has been utilized, and the effects of this extract and its fractions on NO production, iNOS protein expression, and signal pathways involved in its regulation have been evaluated. This study demonstrates that at least some part of the anti-inflammatory activity of the acetonic extract is due to the suppression of NO production by flavone and stearic acid. The mechanism of this inhibition seems to be related to an action on the expression of the enzyme iNOS through the attenuation of nuclear factor kappaB (NF-kappaB) and/or mitogen-activated protein kinase (MAPK) activation. 相似文献
70.
Williams SE Wootton P Mason HS Bould J Iles DE Riccardi D Peers C Kemp PJ 《Science (New York, N.Y.)》2004,306(5704):2093-2097
Modulation of calcium-sensitive potassium (BK) channels by oxygen is important in several mammalian tissues, and in the carotid body it is crucial to respiratory control. However, the identity of the oxygen sensor remains unknown. We demonstrate that hemoxygenase-2 (HO-2) is part of the BK channel complex and enhances channel activity in normoxia. Knockdown of HO-2 expression reduced channel activity, and carbon monoxide, a product of HO-2 activity, rescued this loss of function. Inhibition of BK channels by hypoxia was dependent on HO-2 expression and was augmented by HO-2 stimulation. Furthermore, carotid body cells demonstrated HO-2-dependent hypoxic BK channel inhibition, which indicates that HO-2 is an oxygen sensor that controls channel activity during oxygen deprivation. 相似文献