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Palavecino Pablo Martín Curti María Isabel Bustos Mariela Cecilia Penci María Cecilia Ribotta Pablo Daniel 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(3):326-336
Plant Foods for Human Nutrition - Sorghum is a major cereal crop with various agronomic advantages, contains health-promoting compounds and is gluten-free. There is a growing tendency to use... 相似文献
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The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling 总被引:1,自引:0,他引:1
Hsu PP Kang SA Rameseder J Zhang Y Ottina KA Lim D Peterson TR Choi Y Gray NS Yaffe MB Marto JA Sabatini DM 《Science (New York, N.Y.)》2011,332(6035):1317-1322
The mammalian target of rapamycin (mTOR) protein kinase is a master growth promoter that nucleates two complexes, mTORC1 and mTORC2. Despite the diverse processes controlled by mTOR, few substrates are known. We defined the mTOR-regulated phosphoproteome by quantitative mass spectrometry and characterized the primary sequence motif specificity of mTOR using positional scanning peptide libraries. We found that the phosphorylation response to insulin is largely mTOR dependent and that mTOR exhibits a unique preference for proline, hydrophobic, and aromatic residues at the +1 position. The adaptor protein Grb10 was identified as an mTORC1 substrate that mediates the inhibition of phosphoinositide 3-kinase typical of cells lacking tuberous sclerosis complex 2 (TSC2), a tumor suppressor and negative regulator of mTORC1. Our work clarifies how mTORC1 inhibits growth factor signaling and opens new areas of investigation in mTOR biology. 相似文献
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The application of tannic acid to the elimination of egg stickiness at varied moments of the egg swelling process in pikeperch,Sander lucioperca (L.) 下载免费PDF全文
Daniel Żarski Sławomir Krejszeff Dariusz Kucharczyk Katarzyna Palińska‐Żarska Katarzyna Targońska Krzysztof Kupren Pascal Fontaine Patrick Kestemont 《Aquaculture Research》2015,46(2):324-334
The aim of this article was to analyse the process of pikeperch, Sander lucioperca, egg swelling and to apply tannic acid to eliminate egg stickiness at different moments of the swelling process on artificially obtained eggs. The first experiment involved observation of egg swelling process and the second determined the effect of temperature (12, 14 and 16°C) on the egg swelling rate. The third experiment involved elimination of egg stickiness in a tannin solution (0.75 g L?1) where eggs were submerged in a solution for 0.5, 1, 2 and 5 min – 5, 10, 15, 20, 25 and 30 min following gamete activation. The results indicate that the pikeperch egg swelling process lasts 30 min. It was found that the temperature did not affect the process duration. The results of the third experiment showed that the effectiveness of tannic acid application in egg stickiness elimination increases with time. The best result was obtained in groups of eggs submerged for 1 and 2 min (86.5% and 80.5% of larvae were obtained respectively) 30 min following the gamete activation. The results presented in this study for the first time indicate the possibility of highly effective procedure of egg stickiness elimination with tannic acid in pikeperch aquaculture. 相似文献
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The effects of hydraulic dredging on the benthic infauna of a mud bottom area were investigated in Georgia in the Atlantic Intracoastal Waterway. Complete displacement of the benthic community was caused by dredging; however, the community began to recover within a month following the cessation of dredging. Within two months the diversity and species composition of the dredged channel were similar to that of a control area and little change in sediment composition resulted. Thus, no apparent limitation was imposed on the normal benthic population by habitat alteration. Recolonisation was too rapid to have been caused by the settling of immature or larval stages from the water column alone. Bank slumping and migration of adult forms have been postulated as other means of recolonisation. 相似文献