The humanitarian emergency in Burundi: evaluation of the operational strategy for management of nutritional crisis

OBJECTIVE: To evaluate the impact and appropriateness of programmes for the management and treatment of severe malnutrition in emergency situations. DESIGN: A central unified database was set up with all data and statistics provided by nutritional centres (NC) active in Burundi. SETTING: The paper describes the case of Burundi as an example of the response of the humanitarian community to nutritional crisis. SUBJECTS: Since 1999, more than one million (1,054,210) severely malnourished patients were treated in NC established in Burundi. RESULTS: Peaks of beneficiaries were registered in 2000 and 2001; the admission rate started to decrease in 2002. In 2004, twenty therapeutic feeding centres (TFC) and 224 supplementary feeding centres (SFC) were active for the treatment of 127,420 beneficiaries. Nutritional programmes were present in every province with a coverage rate of 55%. The most convincing impact of the nutritional programme in Burundi was the reduction of mortality rate in children under 5 years of age; an impact on the prevalence of acute malnutrition could not be demonstrated. Children under 5 years old accounted for 62% of beneficiaries in TFC and 76% in SFC. TFC performance indicators fulfilled the minimum standards in disaster response; the performance of SFC was not so optimal with a low recovery rate (69% v. >80%) and a high non-respondent rate (16% v. <5%). With the combination of coverage and cure rate, the programme met 44% of the assessed needs in 2004. CONCLUSIONS: In Burundi the stabilisation of security conditions permitted a combination of humanitarian responses ranging from emergency activities to strengthening of community-based initiatives that could correct the coverage and impact limitations.     

Portable surface plasmon resonance immunosensor for the detection of fluoroquinolone antibiotic residues in milk

An inexpensive and portable surface plasmon resonance (SPR) sensor, SPReeta Evaluation Kit SPR3, has been used to develop a biosensor for the determination of fluoroquinolone antibiotics (FQs) and to demonstrate its performance analyzing FQ residues in milk samples. The SPReeta three-channel gold chips were activated with a mixed self-assembled monolayer (m-SAM) and functionalized with a FQ haptenized protein. Binding of the antibody produced a concentration-dependent increase of the SPR signal as a result of the change in the refraction index. Similarly, the presence of the FQ produced a dose-dependent decrease of the response, which allowed a good limit of detection (LOD) to be obtained (1.0 ± 0.4 μg L(-1) for enrofloxacin in buffer). The response was reproducible in all three channels, on different injections and days, and also between chips. Milk samples could be analyzed after a simple sample treatment involving fat removal by centrifugation and dilution with water. Under these conditions calibration curves were obtained showing that FQ residues can be analyzed in milk samples with an IC(50) value of 26.4 ± 7.2 μg L(-1) and a LOD of 2.0 ± 0.2 μg L(-1) (for enrofloxacin), far below the European Union regulations for this antibiotic family in this matrix. Finally, the paper also demonstrates that the biosensor is able to selectively detect the presence of FQs in milk samples, even in the presence of other antibiotics. Enrofloxacin, ciprofloxacin, and norfloxacin residues were detected in blind samples supplied by Nestle? Co.     

Clinical outcome following pneumonectomy for management of chronic pyothorax in four cats

Pneumonectomy is the resection of all lung lobes from one side of the thorax. The clinical findings, treatment and outcome of four cases of feline chronic pyothorax managed with exploratory thoracotomy and pneumonectomy are reported. All cases were initially medically managed with thoracic drain placement and antibiosis. However, resolution was not achieved with medical therapy and diagnostic imaging findings consistent with an area of abscessation or marked lung lobe consolidation were identified, supporting a decision for surgical management. Surgical exploration was performed via median sternotomy and, on the basis of gross inspection, non-functional lung was removed. A left-sided pneumonectomy was performed in three cats and a right-sided pneumonectomy in one. All cases survived to discharge and an excellent quality of life was reported on long-term follow-up. Pneumonectomy appears to be well tolerated in the cat.     

Validation of Diagnostic Methods for European Foulbrood on Commercial Honey Bee Colonies in the United States

One of the most serious bacterial pathogens of Western honey bees (Apis mellifera Linnaeus [Hymenoptera: Apidae]) is Melissococcus plutonius, the cause of the disease European foulbrood. Because European foulbrood is highly variable, with diverse outcomes at both the individual and colony levels, it is difficult to diagnose through visual inspection alone. Common lab diagnostic techniques include microscopic examination and molecular detection through PCR. In 2009, a lateral flow device was developed and validated for field diagnosis of European foulbrood. At the time, M. plutonius was thought to be genetically homogenous, but we have subsequently learned that this bacterium exists as multiple strains, including some strains that are classified as ‘atypical’ for which the lateral flow device is potentially less effective. These devices are increasingly used in the United States, though they have never been validated using strains from North America. It is essential to validate this device in multiple locations as different strains of M. plutonius circulate in different geographical regions. In this study, we validate the field use of the lateral flow device compared to microscopic examination and qPCR on larval samples from 78 commercial honey bee colonies in the United States with visual signs of infection. In this study, microscopic diagnosis was more sensitive than the lateral flow device (sensitivity = 97.40% and 89.47%, respectively), and we found no false positive results with the lateral flow device. We find high concurrence between the three diagnostic techniques, and all three methods are highly sensitive for diagnosing European foulbrood.     

Seed production in Polylepis australis (Rosaceae) as influenced by tree size, livestock and interannual climate variations in the mountains of central Argentina

The expansion of tree species within a grassland-woodland matrix relies heavily on an abundant seed crop, which may be affected by many factors; among these, browsing by livestock has gone largely ignored. Here we explore how seed production is affected by tree size, grazing by large domestic herbivores and interannual climate fluctuations in a small-sized tree (Polylepis australis, Rosaceae), which dominates the remnant forests in the high mountains of central Argentina. Seed number and seed mass per tree were quantified in 100 different-sized trees located in areas with and without livestock. Using seed traps we also determined seed rain variation in 15 plots between 2005 and 2008. The estimated number of seeds per tree ranged from 0 to 224,000 and increased with tree height (power relation); for trees smaller than 354 cm in height, seed production was lower in areas with livestock and higher in areas without livestock. Mass per seed increased with tree height, with no significant livestock effect. The largest seed rain was recorded in 2008, probably due to growth of seed trees and not in response to climate. To promote seed production and forest expansion, livestock should be excluded from areas with small trees, such as forest edges.     

Drivers of Deforestation in the Lake Victoria Crescent,Uganda

We examined key drivers of deforestation in the Lake Victoria Crescent, Uganda from 1989 to 2009 through a case study approach with a multiple-case design. Nineteen focus groups with local community members and forest officials, archival analysis, and field observation revealed both proximate causes and underlying drivers of deforestation. Proximate causes of deforestation included agricultural expansion into forests, unsustainable extraction of wood forest products, and clearing of forests for nonagricultural uses. Underlying drivers of these proximate causes included policy and institutional factors, economic factors, population growth, technological changes, and changes in culture—each resulting in alienation of local people from forest resources. Alienation of local people, defined as a psychological dispossession of responsibility for forest resources, is posited as the most important underlying driver of deforestation. We discuss the importance of the involvement of local people in the management and maintenance of forests in the Lake Victoria Crescent.     

Shoot development and dieback in progenies of Nothofagus obliqua

Shoot growth and dieback were compared among progenies of nursery-grown seedlings of Nothofagus obliqua belonging to seven progenies of the same provenance (Quila-Quina, Argentina). First-year shoots consisted of one growth unit (GU) and second-year shoots of one or two GUs. The probability of development of two GU was similar for all progenies. Progenies were different in terms of shoot size, terminal bud abscission, the extent of shoot dieback after shoot extension and the node of origin of the relay shoot on the first shoot. Plants with a second-year shoot consisting of two GUs had a thicker stem and more nodes than those with single-GU shoots. The selection of N. obliqua seed trees based on architectural traits suitable for forestry development at specific sites must contemplate variability among progenies and their probabilities of successful development under different conditions.     

Optimal management of a flammable multi-stand forest for timber production and maintenance of nesting sites for wildlife

Decline of cavity-using wildlife species is a major forest management issue. One of the causes of this problem is the loss in cavity tree abundance, resulting from short rotation silviculture, stand-replacing disturbance events and timber harvesting in disturbed stands. Cavity tree availability cannot be guaranteed due to the stochastic nature of disturbance events. We developed a Markov model to predict future cavity tree availability under alternative tree felling and fire protection strategies using information on cavity tree dynamics and fire history. Stochastic dynamic programming was used to find a strategy that maximizes timber revenues less forest management costs, including the cost of an artificial nest-box program that must be implemented whenever cavity trees become critically scarce. The requirement to implement a nest-box program in such circumstances strongly influenced the optimal tree felling strategy and resulted in a higher probability of having cavity trees in the future. This reflected an increase in the retention of old growth forest and stands with fire-killed cavity trees as well as stands of younger trees to provide a future source of cavities. These results demonstrate the need to consider the costs of artificial habitat enhancement and the risk of future cavity tree scarcity in multiple-use forest management planning.     

Rain forest recovery from dieback,Queensland, Australia

We present a detailed account of a dieback episode in tropical rain forest. The dieback episode took place from 1977 to 1989 within a 0.5 ha long-term demography plot monitored for stem growth and mortality from 1975 to 2005. In total 770 m² of rain forest was affected causing 13 trees >10 cm diameter at breast height (dbh) to die, and others to sicken. The dead trees came from four families, though 14 families were represented in the area. Trees of the family Elaeocarpaceae suffered significantly higher mortality. Larger trees were significantly more likely to die than small trees, but smaller trees were more likely to recover. Recruitment to >10 cm dbh size class after dieback was greater in areas that had been affected, and in 2005 stem density and basal area were higher than in 1977, before the dieback episode started. There were no significant trends in biodiversity change between affected and non-affected parts of the plot. Dieback may have dramatic effects at onset but over the medium term its impact appears to be less serious. Longer term monitoring will permit re-evaluation of this observation in the future.