Simplified fat extraction with sulfuric acid as cleanup procedure for residue determination of chlorinated hydrocarbons in butter

A new cleanup procedure is described for chlorinated hydrocarbon residues in butterfat. The method is based on the dropwise addition of H2SO4 to a fat solution column and continuous removal of the lipids and the acid. The cleanup of 0.25-2.0 g fat requires only 10-40 ml sulfuric acid and 12-17 ml petroleum ether. There is no need for any further cleanup step, solvent evaporation, or centrifugation. The method is easy to standardize and is suitable for automation. At least 30 fat samples can be cleaned up manually by one analyst in one day. Recoveries were complete (greater than 90%) for polychlorinated biphenyl compounds and for 13 chlorinated pesticides of 16 examined. The method was tested on chlorinated hydrocarbon residues in commercial butter and the results were compared with those obtained with the acetonitrile method. The versatility and limitations of the method were investigated by varying the sulfuric acid strength, initial fat solution concentration, and column dimensions.     

Estimation of soil strength properties for critical rooting conditions

Methods to aid in the large-scale testing and characterization of Coastal Plain soils based on their susceptibility to root-limiting strength problems were developed and analyzed. They were basically regression equations modeled after a Taylor series expansion. The equations relate changes of soil strength, bulk density and soil water content between field and “critical rooting conditions”. Once equations wered eveloped from a data set of 426 laboratory samples, critical rooting bulk density was predicted for a separate set of laboratory and field samples. All laboratory samples and appropriate field samples were equilibrated at — 100 kPa soil-water potential. Soils used were sandy Ultisols, which may limit the scope of equations.

In many cases, changes in the water contents were not a significant factor in the prediction of soil strength. This may be a reflection of the limited capabilities of the equations, the uniform equilibration of soil-water potential of the soils, or the fact that the slope of the strength vs. bulk density curve is independent of water content over the range of samples considered. Nevertheless, it does simplify the equations and may suggest that a series of several equations for different soil types would be better than a single equation that requires soil-water content.     

Herbivory of an invasive slug in a model grassland community can be affected by earthworms and mycorrhizal fungi

Invasion of non-native species is among the top threats for the biodiversity and functioning of native and agricultural ecosystems worldwide. We investigated whether the herbivory of the slug Arion vulgaris (formerly Arion lusitanicus; Gastropoda), that is listed among the 100 worst alien species in Europe, is affected by soil organisms commonly present in terrestrial ecosystems (i.e. earthworms—Annelida: Lumbricidae and arbuscular mycorrhizal fungi—AMF, Glomerales). We hypothesized that slug herbivory would be affected by soil organisms via altered plant nutrient availability and plant quality. In a greenhouse experiment, we created a simple plant community consisting of a grass, a forb, and a legume species and inoculated these systems with either two earthworm species and/or four AMF taxa. Slugs were introduced after plants were established. Earthworms significantly reduced total slug herbivory in AMF-inoculated plant communities (P?=?0.013). Across plant species, earthworms increased leaf total N and secondary metabolites, AMF decreased leaf thickness. Mycorrhizae induced a shift in slug feeding preference from non-legumes to legumes; the grass was generally avoided by slugs. AMF effects on legume herbivory can partly be explained by the AMF-induced increase in total N and decrease in C/N ratio; earthworm effects are less clear as no worm-induced alterations of legume plant chemistry were observed. The presence of earthworms increased average AMF colonization of plant roots by 140 % (P?<?0.001). Total shoot mass was significantly increased by AMF (P?<?0.001). These data suggest that the feeding behavior of this invasive slug is altered by a belowground control of plant chemical quality and community structure.     

EVALUATION OF POTENTIAL SUBSTRATES TO MONITOR RESPIRATORY NITRATE REDUCTASE ACTIVITY IN SOILS

Different inorganic ions were tested for their ability to replace nitrate as the terminal oxidant for bacterial respiration in anaerobic soils. Chlorate, bromate. selenate. tellurite and ferric ions were all unsuitable substitutes, lodate reduction in soils was similar to nitrate reduction because it required anaerobic conditions, was increased by glucose amendment and decreased by phenylmercuric acetate. Amendment of soils with iodate and measurement of iodide production can be used to measure nitrate reductase activity in a soil and so give a qualitative indication, and possibly a quantitative estimate, of the denitrification capacity of soils.