Evaluating the probiotic potential and adhesion characteristics of Bacillus spp. isolated from the intestine of Rhynchocypris lagowskii Dybowski

Probiotics have been widely used in aquaculture worldwide especially in China as antibiotics have been banned in aquaculture. In the present study, 27 potential strains were isolated from Rhynchocypris lagowskii Dybowski intestinal tract towards the assessment of their probiotic potential. Two potential probiotics were finally screened from the 27 candidate strains according to the results of enzyme-producing ability, in vitro against pathogens ability and antibiotic sensitivity test. The 2 potential probiotics were identified and confirmed on the basis of their colony morphology, biochemical characteristics, and 16S rRNA sequencing. The probiotic strains, LSG2-3–2 and LSG3-6, were identified to be Bacillus methylotrophicus and B. tequilensis, respectively. Further studies showed that LSG2-3–2 and LSG3-6 had excellent tolerance to high temperature (80℃), low pH (3–5), bile salts (0.3%), intestinal juice (10%), and gastric juice (0.5%). The adhesion rates of LSG2-3–2 and LSG3-6 in the in vitro intestinal mucosal adhesion model were 17.74% and 24.04%, respectively. Analysis of their bacteria surface adhesive proteins revealed that the lectins on the bacterial surface of LSG2-3–2 and LSG3-6 were mainly protein and glycoprotein properties, respectively. The adhesion receptor components in the mucus proteins of the two strains were all protein properties. The results of the inhibitory adhesion test indicated that LSG3-6 had a higher inhibitory effect on Aeromonas hydrophila and LSG2-3–2 had a better inhibitory effect on A. veronii. The biosafety assay confirmed that the isolates were not pathogenic to the host fish. Based on the presently observed characteristic features, it can be concluded that LSG2-3–2 (B. methylotrophicus) and LSG3-6 (B. tequilensis) retrieved from the digestive tract of R. lagowskii can be used for the healthy breeding and disease prevention of R. lagowskii.

     

营养条件对海蜇螅状体形成足囊及足囊萌发的影响

用卤虫(Artemia)无节动体作为海蜇(Rhopilema esculenta)螅状幼体的饵料,按不同投饵频次划分为7个营养水平,考察不同营养水平对海蜇螅状体形成足囊及足囊萌发的影响。结果表明,营养条件不仅对螅状体形成足囊的数量有直接影响,而且对足囊萌发也有明显的间接影响。在具备丰富的营养条件下,螅状体形成足囊活跃,个体大,易于萌发;缺乏营养则抑制足囊形成,即使形成足囊,个体也小,不萌发或萌发率很低。     

鱼在腌制过程中水份活度和有关参数的变化关系

本文研究了腌制鱼的水分活度(Aw值)变化及其与其他有关参数的关系。以鲳鱼作为试验原料,用混合腌渍法对鱼品进行不同时间的腌制,然后观察鱼体中水分活度、盐分含量和水分含量的变化情况,并进一步探索这些参数之间相互的变化关系。实验结果表明,鱼体中水分活度和水分含量的降低,盐分的增加均在开始腌制至18小时时为最快;18小时后变化缓慢;34小时后基本趋向平衡。实验表明,水分活度,盐分含量与水分含量之间存在简单的线性关系,因此在腌制过程中,测定出水分和盐分含量就可以计算出水分活度Aw值,从而达到控制鱼品质量的目的。此方法在提高鱼品的保藏效果上具有一定的实用价值。     

Choline requirements of male White Pekin ducks from 21 to 42 d of age

1. A dose–response experiment with 6 dietary choline concentrations (0, 342, 779, 1285, 1662 and 1962 mg/kg) was conducted with male White Pekin ducks to estimate the choline requirement from 21 to 42 d of age.

2. Ninety 21-d-old male White Pekin ducks were allotted to 6 dietary treatments, each containing 5 replicate pens with three birds per pen. At 42 d of age, final weight, weight gain, feed intake and feed/gain were measured. Liver was collected to determine total liver lipid, triglyceride and phospholipids.

3. Significant positive effects of dietary choline on final weight, weight gain and feed intake were observed. In addition, dietary choline supplementation significantly decreased liver lipid and triglyceride content and increased liver phospholipids of Pekin ducks.

4. According to broken-line regression analysis, the choline requirements of male White Pekin ducks from 21 to 42 d of age for weight gain, feed intake and total liver lipid were 980, 950 and 1130 mg/kg. Pekin ducks needed more choline to prevent excess liver lipid deposition than to maintain growth.     

Up-regulated expression of PD-1 and its ligands during acute Classical Swine Fever virus infection in swine

In order to investigate the relationship between the PD-1 pathway and impairment of immune responses with the CSFV infection, the mRNA expression of PD-1 and its ligands were evaluated by quantitative polymerase chain reaction (qPCR) during artificial CSFV infection. Simultaneously, expression of IL-2 and IL-10 mRNA were detected. The T cell proliferation and CSFV load in plasma were also measured. Results showed that the expression of PD-1 and its ligands mRNA were significantly increased (p < 0.01) in PBMC from 3 to 7 days post infection (dpi). Meanwhile the level of IL-10 was up-regulated (p < 0.01). The IL-2 mRNA was not obviously changed but it is significantly increased from 14 dpi. The T cell proliferation was notably decreased at 7 dpi. The CSFV load was also increased in plasma. Overall, our results suggest that the expression of PD-1 and its ligands were up-regulated and probably correlated with immune inhibition during acute CSFV infection.     

关于SBS改性沥青在旧路改造工程上的设计及施工

在良好的设计配合比和施工条件下,SBS沥青能使沥青路面的耐久性和高温稳定性明显提高。本文将根据施工试验情况,论述SBS改性沥青的施工技术要求。     

基于遥感技术的森林可燃物类型划分

应用监督分类法中的最大似然法(Maximum Likelihood Classification)以及遥感图像对塔河林业局森林可燃物类型划分的结果为:针叶林、阔叶林、针阔混交林、空地等几种类型,并绘制了可燃物类型分布图,经分类精度检验,总体分类精度为83.00%,KAPPA系数为0.769。同时,对不同可燃物类型的特点进行了分析。     

Water-use efficiency of a poplar plantation in Northern China

The water-use efficiency (WUE) of an ecosystem—defined as the gross ecosystem production (GEP) divided by the evapotranspiration (ET)—is an important index for understanding the coupling of water and carbon and quantifying water–carbon trade-offs in forests. An open-path eddy covariance technique and a microclimate measurement system were deployed to investigate the WUE of a poplar plantation ecosystem in the Daxing District of Beijing, China, during the growing seasons in 2006, 2007, and 2008. We found that WUE values changed diurnally, peaking in early morning and showing a minimum between 2 pm and 3 pm. This pattern was regulated by photosynthetically active radiation, saturated vapor pressure deficit, and stomatal opening and closure. WUE had inter-daily variations but no substantial seasonal variation. The WUE decreased with increasing soil water content due to the higher sensitivity of ET than GEP to increased soil moisture. Under moist soil conditions (i.e., relative extractable water content >0.4), GEP was stable and WUE was generally low. These results suggest that the poplar plantation does not effectively use the available soil water for carbon uptake, and that soil moisture is lost to the atmosphere through ET.     

黔中地区黄心夜合野生资源现状及其群落物种多样性特征

通过对黔中地区的黄心夜合野生资源进行调查,摸清其资源量、分布情况和生境条件,并对其群落物种多样性特征进行分析,结果显示:(1)黔中地区共有14个黄心夜合野生资源分布地,面积达4万多m2,共有大树454株,幼、小树132株,仅8个点有天然更新情况,资源量少而散,更新能力低;(2)各分布区的生境条件各异,适生范围较广;(3)全区仅有2个黄心夜合群落(乌当区王岗村群落和息烽县天台山群落),其群落多样性变化趋势基本一致,表现为:Shannon-Wiener指数,草本层>灌木层和乔木层;Pielou指数,草本层>灌木层>乔木层;Simpson指数,乔木层≥草本层>灌木层,说明草本层的物种丰富度最高,分布更均匀,乔木和草本的优势种非常明显,灌木层优势种不明显。     

野生胶红酵母糖苷酶水解媚丽新酒中香气糖苷研究

实验分离提取杨凌媚丽葡萄新酒中的香气糖苷,添加优选野生胶红酵母的糖苷酶液,进行催化水解实验,萃取水解生成的品种香气成分,进行GC-MS检测分析。以杏仁β-糖苷酶、黑曲霉果胶酶、La Fort商业果胶酶作催化比较实验。结果表明,杏仁β-糖苷酶标准品催化水解生成的游离香气成分种类和含量最多,其次是野生胶红酵母酶液和黑曲霉果胶酶。酶促水解生成的游离成分中,萜烯类和去甲类异戊二烯化合物能够赋予葡萄酒果香和花香,微量的酚酸酯可增加香气的复杂性,而含量很高的脂肪族类化合物和挥发性酚类会带给葡萄酒不良气味。因此,具有中等催化水解香气糖苷能力的野生胶红酵母的糖苷酶具有良好的增香酿造应用潜力。