不同饲料饲喂断奶仔猪对比试验研究

为了给断奶仔猪选择合适的饲料类型,采用2种类型的饲料对60头35日龄断奶的仔猪进行饲养试验。试验分为2组,即颗粒饲料组和粉状饲料组,每组三个重复,经30 d的饲养试验,结果表明：颗粒饲料组仔猪体质量每日增加数、日头均耗料量明显高于粉状饲料组（P〈0.05）,且颗粒饲料组的经济效益要高于粉状饲料组,而饲料转化效率没有明显差异（P〉0.05）,说明颗粒饲料比粉状饲料更适合饲喂断奶仔猪.     

环青海湖地区禽流感防控工作难点与对策

青海湖是我国最大的内陆咸水湖,又是中亚一印度候鸟迁徙路线上的重要中转站,目前记录到的鸟类有189种.2005年5月青海湖流域发生H5N1型禽流感疫情,造成大量在青海湖繁殖栖息的候鸟死亡.刚察县是环湖重点县,禽流感防控工作面临着严峻的考验,探索出一条符合实际、有效的防控措施势在必行. 1 防控工作难点 多种传染源和传播途经加大禽流感防控困难.禽流感的传染来源较多,可以来自感染或发病的家禽,也可来自野生鸟类、迁徙的水禽等.病毒通过禽类的分泌物、排泄物和尸体及其他物体直接或间接接触发生感染.     

Use of monoclonal antibodies for analyses of Coxiella burnetii major antigens

Monoclonal antibodies (MAbs) to major antigens of Coxiella burnetii were produced. Some of the MAbs to a 62-kDa protein antigen, peptidoglycan protein complex and lipopolysaccharide (LPS) O-chains reacted with other bacteria whereas none of the MAbs to outer membrane proteins and LPS outer-core did. The LPS outer-core and OMPs may be useful antigens for specifically detecting antibodies to C. burnetii.     

协力构建和谐的医患关系

和谐的医患关系是社会主义和谐社会的重要内容.构建和谐的医患关系,需要政府、医院、医务人员和患方的共同努力.为此,政府应增加卫生经费投入,加快卫生立法步伐;医院应着力医德医风建设,正确处理医疗纠纷;医务人员应树立人文关怀理念,改善医疗服务态度;患方应科学认识就医行为,理性对待医疗事故.     

复合双歧杆菌制剂对肉羊生长性能、养分表观消化率及血液生化指标的影响

为研究复合双歧杆菌制剂对肉羊生长性能、养分表观消化率及血液生化指标的影响,试验选择健康、体重相近的杜寒杂交肉羊60只随机分成4组,每组15个重复,每个重复1只,1组为对照组饲喂基础日粮,试验2、3、4组分别在基础日粮中添加2.5％、5.0％、10％的复合双歧杆菌制剂,预试验7 d,试验期56 d.结果表明:(1)试验3...     

建立健全新型动物防疫体系的若干思考

建设和完善动物防疫体系,提高动物疫病的防控能力,是实现畜牧业健康稳定发展的前提.《中共中央关于制定国民经济和社会发展第十四个五年规划和二〇三五年远景目标的建议》提出:健全动物防疫和农作物病虫害防治体系,为建立健全新型动物防疫体系强化的政策基础.本文重点从机构设置、人员结构、工资待遇等层面阐述了机构改革后动物防疫体系的发...     

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised aglandular areas called caruncular (C), and intensely glandular intercaruncular areas (IC). In order to better understand the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins (DEPs) (P < 0.05), functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on. Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

高温应激诱导奶牛乳腺细胞生长周期阻滞

实验通过台盼蓝染色、MTT、细胞流式等方法研究高温(40℃/41℃)对乳腺细胞活性、细胞周期及凋亡的影响。结果表明,高温可以抑制乳腺细胞的增殖,使细胞产生S、G2期阻滞,诱导乳腺细胞凋亡,并随高温强度的增加而作用增强。试验提示高温可诱导乳腺细胞周期阻滞,抑制细胞增殖。     

葡萄糖氧化酶产生菌的快速筛选及其产酶条件的研究

通过平板快速筛选方法从土壤样品中分离得到一株产葡萄糖氧化酶菌株,此菌株属于产胞内酶的黑曲霉。采用文献报道的相关工艺测得其初始酶活为0.86μmol/min。经过培养基配方的优化试验及摇床发酵工艺的调整,其摇床发酵的产酶率有了较大的提高,发酵液过滤液酶活达到9.91μmol/min。