全文获取类型
收费全文 | 441篇 |
免费 | 17篇 |
国内免费 | 1篇 |
专业分类
林业 | 40篇 |
农学 | 13篇 |
135篇 | |
综合类 | 46篇 |
农作物 | 29篇 |
水产渔业 | 36篇 |
畜牧兽医 | 106篇 |
园艺 | 6篇 |
植物保护 | 48篇 |
出版年
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 14篇 |
2020年 | 14篇 |
2019年 | 7篇 |
2018年 | 13篇 |
2017年 | 12篇 |
2016年 | 17篇 |
2015年 | 8篇 |
2014年 | 9篇 |
2013年 | 15篇 |
2012年 | 28篇 |
2011年 | 45篇 |
2010年 | 16篇 |
2009年 | 12篇 |
2008年 | 48篇 |
2007年 | 28篇 |
2006年 | 26篇 |
2005年 | 34篇 |
2004年 | 23篇 |
2003年 | 35篇 |
2002年 | 22篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
排序方式: 共有459条查询结果,搜索用时 46 毫秒
121.
122.
Long-term organic farming fosters below and aboveground biota: Implications for soil quality, biological control and productivity 总被引:3,自引:1,他引:3
Klaus Birkhofer T. Martijn Bezemer Jaap Bloem Michael Bonkowski Sren Christensen David Dubois Fleming Ekelund Andreas Fließbach Lucie Gunst Katarina Hedlund Paul Mder Juha Mikola Christophe Robin Heikki Setl Fabienne Tatin-Froux Wim H. Van der Putten Stefan Scheu 《Soil biology & biochemistry》2008,40(9):2297-2308
Organic farming may contribute substantially to future agricultural production worldwide by improving soil quality and pest control, thereby reducing environmental impacts of conventional farming. We investigated in a comprehensive way soil chemical, as well as below and aboveground biological parameters of two organic and two conventional wheat farming systems that primarily differed in fertilization and weed management strategies. Contrast analyses identified management related differences between “herbicide-free” bioorganic (BIOORG) and biodynamic (BIODYN) systems and conventional systems with (CONFYM) or without manure (CONMIN) and herbicide application within a long-term agricultural experiment (DOK trial, Switzerland). Soil carbon content was significantly higher in systems receiving farmyard manure and concomitantly microbial biomass (fungi and bacteria) was increased. Microbial activity parameters, such as microbial basal respiration and nitrogen mineralization, showed an opposite pattern, suggesting that soil carbon in the conventional system (CONFYM) was more easily accessible to microorganisms than in organic systems. Bacterivorous nematodes and earthworms were most abundant in systems that received farmyard manure, which is in line with the responses of their potential food sources (microbes and organic matter). Mineral fertilizer application detrimentally affected enchytraeids and Diptera larvae, whereas aphids benefited. Spider abundance was favoured by organic management, most likely a response to increased prey availability from the belowground subsystem or increased weed coverage. In contrast to most soil-based, bottom-up controlled interactions, the twofold higher abundance of this generalist predator group in organic systems likely contributed to the significantly lower abundance of aboveground herbivore pests (aphids) in these systems. Long-term organic farming and the application of farmyard manure promoted soil quality, microbial biomass and fostered natural enemies and ecosystem engineers, suggesting enhanced nutrient cycling and pest control. Mineral fertilizers and herbicide application, in contrast, affected the potential for top-down control of aboveground pests negatively and reduced the organic carbon levels. Our study indicates that the use of synthetic fertilizers and herbicide application changes interactions within and between below and aboveground components, ultimately promoting negative environmental impacts of agriculture by reducing internal biological cycles and pest control. On the contrary, organic farming fosters microbial and faunal decomposers and this propagates into the aboveground system via generalist predators thereby increasing conservation biological control. However, grain and straw yields were 23% higher in systems receiving mineral fertilizers and herbicides reflecting the trade-off between productivity and environmental responsibility. 相似文献
123.
Successful engraftment of cultured autologous mesenchymal stem cells in a surgically repaired soft palate defect in an adult horse 总被引:6,自引:0,他引:6
下载免费PDF全文
![点击此处可从《Canadian journal of veterinary research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Bianca Carstanjen Christophe Desbois Mehrak Hekmati Luc Behr 《Canadian journal of veterinary research》2006,70(2):143-147
The objective of this study was to graft autologous mesenchymal stem cells (MSCs) at the site of surgical repair of a soft palate defect in an adult horse in an attempt to improve wound healing and to investigate whether the transplanted MSCs would integrate into the soft palate structure and participate in regeneration. Bone marrow was collected from an adult horse with a full-thickness soft palate defect. The MSCs were isolated, cultured in monolayers, and labeled with 5-bromo-2-desoxymidine (BrdU) and chloromethylbenzamido-DiI-derived (cm-DiI) before transplantation. The soft palate defect was repaired by mandibular symphysiotomy, and the labeled MSCs were injected into the repaired soft palate. Postmortem examination revealed that 90% of the soft palate defect had been sutured. Staining by BrdU and cm-DiI was intense in the soft palate tissue. Labeled MSCs were detected in tissue slices from the injection sites. The cells were organized in a manner similar to that in native soft palate tissue, indicating successful engraftment. 相似文献
124.
Site fidelity and movements of an amphidromous goby revealed by otolith multi‐elemental signatures along a tropical watershed
下载免费PDF全文
![点击此处可从《Ecology of Freshwater Fish》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Nils Teichert Helene Tabouret Raphaël Lagarde Henri Grondin Dominique Ponton Christophe Pécheyran Gilles Bareille 《Ecology of Freshwater Fish》2018,27(3):834-846
Otolith microchemistry can provide crucial information to address gaps of knowledge in spatio‐temporal ecology of fish species. However, understanding the seasonal variability of water chemistry and its effect on otolith signatures is needed to interpret fish movements. Otolith multi‐elemental signatures were used to examine the diadromous migration and small‐scale movements of a tropical goby (Sicyopterus lagocephalus) within a watershed in La Réunion Island, Indian Ocean. The elemental ratios Sr:Ca, Ba:Ca and Sr:Ba recorded monthly in the water of nine sampling sites varied between localities and were correlated with otolith signatures, whereas the Mg:Ca signature was not. The incorporation of some trace elements in otoliths was influenced by the size of fish and site where they were caught, reflecting an effect of endogenous and exogenous factors. Despite these sources of variation, the elemental signatures did not overlap for three river sections distributed along the 26‐km‐long watershed. The diadromous migration and fish lifetime movements between these river sections were reconstructed using an original process based on random forest analysis. This approach revealed distinct migratory behaviours after fish entered in the river, with some individuals progressing slowly in the watershed whereas other rapidly reached the upper localities. As adults, S. lagocephalus showed a restricted exploratory behaviour and a strong site fidelity to a particular part of the watershed. The detection of adult movement was typically only between adjacent locations and is possibly explained by shifts in local intraspecific hierarchies and/or displacement during extreme discharge caused by cyclones. 相似文献
125.
Croucher NJ Harris SR Fraser C Quail MA Burton J van der Linden M McGee L von Gottberg A Song JH Ko KS Pichon B Baker S Parry CM Lambertsen LM Shahinas D Pillai DR Mitchell TJ Dougan G Tomasz A Klugman KP Parkhill J Hanage WP Bentley SD 《Science (New York, N.Y.)》2011,331(6016):430-434
Epidemiological studies of the naturally transformable bacterial pathogen Streptococcus pneumoniae have previously been confounded by high rates of recombination. Sequencing 240 isolates of the PMEN1 (Spain(23F)-1) multidrug-resistant lineage enabled base substitutions to be distinguished from polymorphisms arising through horizontal sequence transfer. More than 700 recombinations were detected, with genes encoding major antigens frequently affected. Among these were 10 capsule-switching events, one of which accompanied a population shift as vaccine-escape serotype 19A isolates emerged in the USA after the introduction of the conjugate polysaccharide vaccine. The evolution of resistance to fluoroquinolones, rifampicin, and macrolides was observed to occur on multiple occasions. This study details how genomic plasticity within lineages of recombinogenic bacteria can permit adaptation to clinical interventions over remarkably short time scales. 相似文献
126.
Philippot P Van Zuilen M Lepot K Thomazo C Farquhar J Van Kranendonk MJ 《Science (New York, N.Y.)》2007,317(5844):1534-1537
Microscopic sulfides with low 34S/32S ratios in marine sulfate deposits from the 3490-million-year old Dresser Formation, Australia, have been interpreted as evidence for the presence of early sulfate-reducing organisms on Earth. We show that these microscopic sulfides have a mass-independently fractionated sulfur isotopic anomaly (Delta33S) that differs from that of their host sulfate (barite). These microscopic sulfides could not have been produced by sulfate-reducing microbes, nor by abiologic processes that involve reduction of sulfate. Instead, we interpret the combined negative delta34S and positive Delta33S signature of these microscopic sulfides as evidence for the early existence of organisms that disproportionate elemental sulfur. 相似文献
127.
Barrangou R Fremaux C Deveau H Richards M Boyaval P Moineau S Romero DA Horvath P 《Science (New York, N.Y.)》2007,315(5819):1709-1712
Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity. 相似文献
128.
Yu H Braun P Yildirim MA Lemmens I Venkatesan K Sahalie J Hirozane-Kishikawa T Gebreab F Li N Simonis N Hao T Rual JF Dricot A Vazquez A Murray RR Simon C Tardivo L Tam S Svrzikapa N Fan C de Smet AS Motyl A Hudson ME Park J Xin X Cusick ME Moore T Boone C Snyder M Roth FP Barabási AL Tavernier J Hill DE Vidal M 《Science (New York, N.Y.)》2008,322(5898):104-110
Current yeast interactome network maps contain several hundred molecular complexes with limited and somewhat controversial representation of direct binary interactions. We carried out a comparative quality assessment of current yeast interactome data sets, demonstrating that high-throughput yeast two-hybrid (Y2H) screening provides high-quality binary interaction information. Because a large fraction of the yeast binary interactome remains to be mapped, we developed an empirically controlled mapping framework to produce a "second-generation" high-quality, high-throughput Y2H data set covering approximately 20% of all yeast binary interactions. Both Y2H and affinity purification followed by mass spectrometry (AP/MS) data are of equally high quality but of a fundamentally different and complementary nature, resulting in networks with different topological and biological properties. Compared to co-complex interactome models, this binary map is enriched for transient signaling interactions and intercomplex connections with a highly significant clustering between essential proteins. Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy. 相似文献
129.
Toudic B Garcia P Odin C Rabiller P Ecolivet C Collet E Bourges P McIntyre GJ Hollingsworth MD Breczewski T 《Science (New York, N.Y.)》2008,319(5859):69-71
Numerous crystalline materials, including those of bioorganic origin, comprise incommensurate sublattices whose mutual arrangement is described in a superspace framework exceeding three dimensions. We report direct observation by neutron diffraction of superspace symmetry breaking in a solid-solid phase transition of an incommensurate host-guest system: the channel inclusion compound of nonadecane/urea. Strikingly, this phase transition generates a unit cell doubling that concerns only the modulation of one substructure by the other-an internal variable available only in superspace. This unanticipated pathway for degrees of freedom to rearrange leads to a second phase transition, which again is controlled by the higher dimensionality of superspace. These results reveal nature's capacity to explore the increased number of phases allowed in aperiodic crystals. 相似文献
130.
Genetic surveys reveal the diversity of bacteria and lead to the questioning of species concepts used to categorize bacteria. One difficulty in defining bacterial species arises from the high rates of recombination that results in the transfer of DNA between relatively distantly related bacteria. Barriers to this process, which could be used to define species naturally, are not apparent. Here, we review conceptual models of bacterial speciation and describe our computer simulations of speciation. Our findings suggest that the rate of recombination and its relation to genetic divergence have a strong influence on outcomes. We propose that a distinction be made between clonal divergence and sexual speciation. Hence, to make sense of bacterial diversity, we need data not only from genetic surveys but also from experimental determination of selection pressures and recombination rates and from theoretical models. 相似文献