Fish meal replacement by plant and animal by-products in diets for the Australian short-finned eel, Anguilla australis australis (Richardson)

In this experiment, 23% of fish meal protein was replaced with protein from Australian soybean meal (SBM), lupin meal (LM), corn gluten meal (CGM) and meat meal (MM) in nutritionally balanced experimental diets. Growth and growth efficiencies of juvenile Australian short‐finned eel Anguilla australis australis (Richardson) elvers (2.23±0.4‐g average wet weight) were compared. Elvers were fed twice a day to a total of 5% body weight per day for 63 days. Total weight gain (g), specific growth rate (% day^?1), protein efficiency ratio (%) and productive protein value (%) were significantly higher (P<0.05) for elvers fed the MM diet than for those fed the LM diet. There were no differences in diurnal ammonia‐nitrogen excretion rates. However, diurnal urea‐nitrogen excretion rates were significantly (P<0.05) affected by diet and rates were two to three times higher for all diets, except CGM, 4 h following the afternoon feed. Diet significantly affected apparent digestibility (AD); ADs of protein for SBM and LM were significantly (P<0.001) higher than for control diet, CGM and MM; AD of energy for LM was significantly (P<0.05) lower than for all the other diets. Fish meal protein was replaced by CGM, SBM and MM without compromising growth rates of the Australian short‐finned eel. However, whole lupin seed meal (LM) depressed growth and growth efficiencies most likely because of the high indigestible carbohydrate content.     

Fish and flows: Abiotic drivers influence the recruitment response of a freshwater fish community throughout a regulated lotic system of the Murray-Darling Basin,Australia

1. Fish assemblages in dryland rivers have life-history strategies that have evolved in response to environmental conditions and triggers, particularly water temperatures and flow regimes. The regulation of rivers through the construction of dams, weirs and other water diversion structures has altered natural flow regimes and the associated ecological processes of river systems worldwide.
2. Over a 3-year period, using standardized fish sampling and daily otolith ageing, the recruitment of eight freshwater fish species was monitored in response to various abiotic drivers, including hydrology and water temperatures, throughout the Macquarie River, a large regulated river system of the Australian Murray-Darling Basin.
3. A data-driven statistical classification system is provided that groups species into reproductive guilds, based on their recruitment response to hydrology and water temperature, specifically designed for use in environmental flow management.
4. The eight species were grouped into three distinct reproductive guilds that showed similar recruitment responses to the abiotic drivers. Murray-Darling rainbowfish, Murray cod, and eel-tailed catfish were considered as a single guild, characterized by seasonal recruitment during a relatively narrow thermal window under low to moderate stable discharges. The second group included common carp, bony herring, and golden perch, which recruited primarily in association with larger flow events. Un-specked hardyhead and Australian smelt formed a more differentiated guild, recruiting over a broad range of temperatures and discharges. Limitations associated with using a reproductive guild approach to simplify water management are discussed.
5. This study highlighted important relationships among hydrology, water temperatures, and successful recruitment that can, in turn, be used to inform development of adaptive flow management plans and effective use of environmental water for the conservation management of native fish communities. Important considerations in the design of studies that aim to examine relationships between recruitment and abiotic drivers are also discussed.

     

Hypotheses to explain patterns of population change among breeding bird species in England

Population declines are now evident across many taxa, but within each assemblage there is often considerable variation in constituent population trends. We used bird population trends produced from the BTO/JNCC Common Birds Census (CBC) and the RSPB/BTO/JNCC Breeding Bird Survey (BBS), to test five main hypotheses to explain population changes of 59 breeding species in England (1967-2006): (1) breeding habitat, (2) predation risk to nest sites, (3) species climatic niche, (4) migration strategy, and (5) over-wintering bioclimatic zones of migrants, accounting for additional demographic and ecological traits. In absence of phylogenetic inter-relatedness, farmland species declined more than woodland species, most pronounced prior to 1986, probably reflecting agricultural intensification (1). We found limited support that ground nesters have declined more than above-ground or cavity nesters (2), and there was some indication that species with more northerly European distributions showed larger declines than more southerly-distributed species (3). Larger population declines were recorded for Afro-tropical migrants than species wintering in Europe or in the UK, most notable prior to 1986 (4). However, declines were not uniform across all migrants (5) - species over-wintering in the arid savannah bioclimatic zone of Africa decreased in population between 1967 and 1976, whereas species wintering in humid West African forest and savannah declined more after 1987. These results suggest both breeding and over-wintering factors influenced population trends. European countries signed to the Convention on the Conservation of Migratory Species of Wild Animals are required to protect and conserve populations of migrants. Understanding connectivity between breeding and over-wintering populations, and similar environmental pressures experienced within over-wintering areas may be a useful step towards mitigating against further declines in migrants.     

Sample size and power determination for detecting interactions in mixtures of chemicals

In the analysis of mixtures of drugs/chemicals it is often of interest to test for the presence of interaction. If the hypothesis of no interaction (additivity) is not rejected, then the analyst may reasonably claim additivity if and only if the study is powered to a desired (e.g., biologically meaningful) level. The objective of this article is to address the sample size and power issues related to testing the hypothesis of additivity at specified mixture points. The study of disinfectant by-products (DBPs) found in drinking water, described in earlier literature, is used to illustrate the procedures for estimating power and sample sizes for detecting interactions at specified mixtures. The four trihalomethanes used in the study are bromodichloromethane (BDCM), chlorodibromomethane (CDBM), chloroform (CHCl₃), and bromoform (CHBr₃)     

16alpha-Bromo-epiandrosterone therapy modulates experimental feline immunodeficiency virus viremia: initial enhancement leading to long-term suppression

16alpha-Bromo-epiandrosterone (epiBr), a synthetic derivative of the natural hormone dehyroepiandrosterone (DHEA), was evaluated for its effects on feline immunodeficiency virus (FIV) infection in experimental cats. The rationale for this study was based on the ability of DHEA to significantly reduce the mortality to viral infections in mice. DHEA and epiBr also have demonstrable in vitro anti-viral activity for both HIV-1 and FIV. Preliminary pharmacokinetic studies in cats demonstrated that subcutaneously injected epiBr was rapidly absorbed, completely metabolized, and nontoxic. Metabolites were excreted in both urine and feces, with the latter having the most complex pattern of breakdown products. Cats were then divided into four groups; two groups were infected with FIV and two uninfected. Two groups, one infected and one uninfected were treated on 5 consecutive days of weeks 0, 4, 8, 12 and 16 with epiBr. The remaining two groups were mock treated with the drug vehicle alone. Treatment started 1 week prior to infection and extended for 4 weeks after infection. Cats were observed for 20 weeks post-FIV infection. Infected cats had identical decreases in blood neutrophil and lymphocyte counts following, regardless of whether they were treated with epiBr or vehicle alone. The CD4/CD8 T-cell ratio was decreased following FIV exposure, but was significantly more decreased for the epiBr treated animals from week 2 post-infection onward. CD4+ T cells were decreased in FIV-infected cats treated with epiBr compared to their untreated cohort, while CD8+ T cells tended to be higher in treated animals. FIV infected cats that were treated with epiBr had over one-log higher virus loads at week 2 post-infection than non-epiBr treated cohorts. In spite of this enhanced initial viremia, the subsequent levels of virus in the blood were significantly lower in epiBr treated versus untreated animals. EpiBr treated cats had significantly higher FIV-p24 antibody responses than control cats receiving vehicle alone, although primary and secondary antibody responses to a T-cell dependent non-FIV antigen, keyhole limpet hemocyanin (KLH), were unaffected. EpiBr treatment significantly decreased the expected FIV-induced suppression of IL-12 p40 mRNA levels in peripheral blood mononuclear cells (PBMCs) observed at weeks 4, 5, 8, 9 and 16 post-infection, but had no influence on FIV-induced changes in IL-4, IL-6, IL-10, IFN-gamma, MIP-1alpha and RANTES.     

Effect of processing conditions on trace elements in fish roe from six commercial new zealand fish species

The concentrations of trace elements in fish roes and the effect of processing conditions (karasumi-like or karashi mentaiko) were investigated in six commercial fish species from New Zealand. The studied elements were As, Cd, Cr, Cu, Hg, Pb, and Zn, and the roes were from the following species: chinook salmon ( Oncorhynchus tshawytscha), hoki ( Macruronus novaezelandiae), southern blue whiting ( Micromesistius australis), hake ( Merluccius australis), blue warehou ( Seriolella brama), and barracouta ( Thyrsites atun). The concentrations of As, Cd, Cr, Hg, and Pb in the roes were lower than literature values for fish muscles. Only Zn in barracouta roe and Cu in salmon roe and their products were relatively higher than the generally accepted levels in fish muscles and could be of safety concern. Hence, the consumption of barracouta and salmon roes among certain parts of the population needs to be monitored and assessed. Dry salting (karasumi-like) processing increased ( P < 0.001) the concentrations of the studied trace elements while salting fermentation (karashi mentaiko) processing tended to decrease the levels of trace elements. Fermentation may be a useful process to decrease the level of toxic trace elements.     

Simulation of the soil-water dynamics and corn yields under deficit irrigation

Summary A simulation model capable of predicting the yield response of corn to a limited water supply was developed by combining two existing mathematical models. The resulting computer model was evaluated using experimental data taken under a wide range of soil moisture conditions. The soil profile water balances was simulated using SWATRE and SUCROS was used to model the crop growth in response to environmental conditions. In addition to the integration of the two existing models, some minor changes were made to each in an effort to improve the accuracy of the combined models. The model input parameters were derived entirely from published literature. The experimental data necessary for model validation were available from irrigation studies at the Sandhills Agricultural Laboratory of the University of Nebraska. These experiments not only provided the required input soil and climatic data, but also the observed irrigation levels, soil moisture distributions and crop yield required for model validation. Initial evaluation of the computer model indicates that the combined model adequately describes crop evapotranspiration, soil moisture extraction and crop yield under a fairly wide range of soil moisture stress. Additional modifications for the prediction of leaf area expansion and senescence, especially under moisture stress, are needed to improve the accuracy of the model.