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121.
Rolef Ben-Shahar T Heeger S Lehane C East P Flynn H Skehel M Uhlmann F 《Science (New York, N.Y.)》2008,321(5888):563-566
Replicated chromosomes are held together by the chromosomal cohesin complex from the time of their synthesis in S phase onward. This requires the replication fork-associated acetyl transferase Eco1, but Eco1's mechanism of action is not known. We identified spontaneous suppressors of the thermosensitive eco1-1 allele in budding yeast. An acetylation-mimicking mutation of a conserved lysine in cohesin's Smc3 subunit makes Eco1 dispensable for cell growth, and we show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion. A second set of eco1-1 suppressors inactivate the budding yeast ortholog of the cohesin destabilizer Wapl. Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent. 相似文献
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Although classical genetic and biochemical approaches have identified hundreds of proteins that function in the dynamic remodeling of cell shape in response to upstream signals, there is currently little systems-level understanding of the organization and composition of signaling networks that regulate cell morphology. We have developed quantitative morphological profiling methods to systematically investigate the role of individual genes in the regulation of cell morphology in a fast, robust, and cost-efficient manner. We analyzed a compendium of quantitative morphological signatures and described the existence of local signaling networks that act to regulate cell protrusion, adhesion, and tension. 相似文献
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Soil quality and financial performance of biodynamic and conventional farms in new zealand 总被引:3,自引:0,他引:3
Biodynamic farming practices and systems show promise in mitigating some of the detrimental effects of chemical-dependent, conventional agriculture on the environment. The physical, biological, and chemical soil properties and economic profitability of adjacent, commercial biodynamic and conventional farms (16 total) in New Zealand were compared. The biodynamic farms in the study had better soil quality than the neighboring conventional farms and were just as financially viable on a per hectare basis. 相似文献
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Smartt SJ Maund JR Hendry MA Tout CA Gilmore GF Mattila S Benn CR 《Science (New York, N.Y.)》2004,303(5657):499-503
We present the discovery of a red supergiant star that exploded as supernova 2003gd in the nearby spiral galaxy M74. The Hubble Space Telescope (HST) and the Gemini Telescope imaged this galaxy 6 to 9 months before the supernova explosion, and subsequent HST images confirm the positional coincidence of the supernova with a single resolved star that is a red supergiant of 8(+4)(-2) solar masses. This confirms both stellar evolution models and supernova theories predicting that cool red supergiants are the immediate progenitor stars of type II-plateau supernovae. 相似文献
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Harris AC Kamenetsky VS White NC van Achterbergh E Ryan CG 《Science (New York, N.Y.)》2003,302(5653):2109-2111
At a porphyry copper-gold deposit in Bajo de la Alumbrera, Argentina, silicate-melt inclusions coexist with hypersaline liquid- and vapor-rich inclusions in the earliest magmatic-hydrothermal quartz veins. Copper concentrations of the hypersaline liquid and vapor inclusions reached maxima of 10.0 weight % (wt %) and 4.5 wt %, respectively. These unusually copper-rich inclusions are considered to be the most primitive ore fluid found thus far. Their preservation with coexisting melt allows for the direct quantification of important oreforming processes, including determination of bulk partition coefficients of metals from magma into ore-forming magmatic volatile phases. 相似文献