鸭疫里氏杆菌免疫原性研究Ⅰ.荚膜的形态学观察及荚膜物质的提取

本研究应用电镜对Ⅰ型鸭疫里氏杆菌的荚膜进行了初步观察,在电镜下,负染的菌体周围呈一电子透明层;未经抗体处理的细菌超薄切片,荚膜在菌体周围呈松散的絮状结构,而应用同源抗体处理后,荚膜则呈一均匀层。同时在超薄切片中还看到细菌的空泡和外膜结构。采用十六烷基三甲基溴化铵（ＣＴＡＢ）成功地提取了培养物上清液中的荚膜成分,经过测定比较,加入５ｍＭＣＴＡＢ时效果最佳。     

新城疫病毒糖蛋白致病作用的研究

本实验以分子生物学和现代免疫学方法,对新城疫病毒（ＮＤＶ）中国毒株Ｆ４８Ｅ９和ＬａＳｏｔａ等毒株的糖蛋白的致病作用进行了探讨。结果表明,ＮＤＶ Ｆ和ＨＮ蛋白为糖蛋白,强弱毒株之间Ｆ蛋白裂解位点的氨基酸序列有明显的不同,并决定了毒株的毒力,但ＮＤＶ的致病作用尚需ＨＮ、Ｆ蛋白的协同作用,单一的Ｆ或ＨＮ蛋白不能构成ＮＤＶ的致病作用。     

用发酵罐制备嗜水气单胞菌疫苗用菌液培养条件的优化

本试验探讨了用发酵罐培养嗜水气单胞菌灭活疫苗生产菌株Ｊ－１株菌液的最佳条件。分别以发酵罐和摇床在不同的培养条件下培养Ｊ－１株制备菌液,前者１２个批次,后者６个批次。比较不同的培养条件对细菌菌体数量及ＨＥＣ毒素产量的影响。结果表明：发酵罐培养优于摇床培养,以产毒素培养基于２８℃发酵罐通气培养Ｊ－１株,培养２８小时效果最佳,培养菌液含菌量达２．９×１０１０ｃｆｕ／ｍｌ,培养液上清的溶血价达２７,发酵液中残糖为０．１２ｇ／Ｌ,符合发酵工艺要求。     

草麻黄的组织培养及植株再生

将草麻黄幼苗节间幼茎部分作外植体,培养在附加KT 0.054mg/l+2,4-D 1.11mg/l的MS培养基中,可成功诱导出愈伤组织,愈伤组织分化出芽;同样的外植体培养在附加BA3mg/l+IAA0.2mg/l的MS培养基中,诱导出大量不定芽;不同途径得到的芽转移到MS附加6-BA 0.056mg/l+1.11mg/l2,4-D的培养基后,可分化出不定根,从而形成完整植株.     

土壤低温对嫁接黄瓜生理生化特性的影响

黄瓜嫁接及自根植株在结果初期经１１～１３℃土壤低温处理１周后,根系中酶的活性降低,根系生长受到抑制,伤流速率降低,伤流液中矿质元素含量明显减少,地上部生长也受到抑制,叶绿素含量减少,叶片水势降低,气孔阻力和细胞间隙ＣＯ２浓度增大,光合速率和蒸腾速率降低。嫁接植株各项测定指标均优于自根植株。由此得出,土壤低温通过根系对整个植株产生了不良影响;嫁接能够增强根系抗土壤低温的能力。     

Biomass evaluation of concealed insect pests at parasitism based on allometric scaling laws

In general, it is difficult to evaluate host resource utilization of parasitoids by examining the biomass of concealed wood borers at parasitism, because the larval tissues of parasitized hosts observed in field surveys are often almost completely consumed. This work explored a precise and convenient method for evaluating the biomass of concealed wood-boring insect pests after they are parasitized. Allometric scaling laws were used to determine mathematical relationships between larval weight and other size variables in two host insects: the emerald ash borer [EAB], Agrilus planipennis Fairmaire, and the oak long-horned beetle [OLB], Massicus raddei (Blessig). These insects are hosts of the parasitoid Sclerodermus pupariae Yang et Yao (Hymenoptera: Bethylidae). Our results showed that body weight among different instars was significantly different for both host species, with greater overlap between neighboring instars. Mean larval weight had a highly significant exponential relationship with instar number. EAB larval weight showed a highly significant power relationship to body length and width, width of the prothoracic plate, peristoma width, urogomphus length, and anteriormost width of the urogomphus. Significant power relationships were also observed between OLB larval weight and body size parameters (including body length, length of the mesothoracic spiracle, width of the prothoracic plate, distance between the main ocelli, and mandible length). These findings indicated that host biomass could be easily calculated if any one size variable could be measured at the time of parasitism. Allometric methods provide a precise means of evaluating time-specific biomass of concealed wood borers.     

鲜食和加工兼用品种新胡萝卜1号的选育

新胡萝卜１号是从当地农家品种自然混杂后代中经过近１０ａ（年）选育而成的一个新品种。植株生长势较强，肉质根粗圆柱形，长１４～１６ｃｍ，粗４～５ｃｍ，单根质量１２０～１４０ｇ。肉质根皮色、肉色和心柱色都为橙红色且群体“三红”比率达９５％以上，适合于鲜食和加工，其质脆味甜，水分适中，综合性状均优于原始亲本和当地其它生产用品种。该品种平均产量达６４．５ｔ·ｈｍ－２，适应性强，目前已在新疆、陕西和四川等地推广２００ｈｍ２以上。     

Quantitative dynamics on stimulating regeneration and sowing seedlings of Larix gmelinii in Daxing''''an Mountains

Introduction4LarixgmeliniiisoneofmajortimberspeciesinthenortheastDaxing'anMountainsinChina.Itplaysanimportantroleinprovidinglumber,maintainingthenaturalenvironmentinthisregionandadjustingecologicalequilibrium.Alargenumberofburnedareasandcut-overareasneedtoregenerateeveryyear.Larixgmeliniihasgreatnaturalregenerationcapabilityandenoughseedsources,andalmostmeetsabumperharvestyeareverythreeyears(Xuetal.1992;Shan1990;Xuetal.1993).Someburnedandloggedareaswouldberapidlyrestoredthroughafewofassista…     

Comparison of Blood Physiological and Biochemical Parameters of Two Kinds of Minipigs in Different Altitudes

This study was designed to compare the blood physiological and biochemical parameters between plateau Juema minipigs and plain Bama minipigs,and provide a theoretical basis for Juema minipigs served as experimental animals in the future.The blood samples of Juema minipigs were taken in Gannan,and the blood samples of the Bama minipigs were taken in Xi'an,24 blood physiological and biochemical parameters were tested using an automatic analyzer of blood cells and biochemistry.Between the plateau Juema minipigs and Bama minipigs,there were significant differences in PLT,RDW-CV and RDW-SD (P< 0.05) and extremely significant differences in RBC,HGB,HCT,MCH,MCMH,GGT,GLU and CRE (P< 0.01).The plateau Juema minipig had an altitude acclimatization,so it was better than Bama minipig in plateau medical science.     

2007年11月全国饲料生产情况分析

1生产总况 2007年11月，国内饲料生产形势有所好转。进入11月国内大部分地区的气温明显下降，促进了肉类的消费。而生猪价格已经接近甚至超过了7、8月份的最高水平。从消费方面看，已经进入元旦、春节消费高峰期，后期市场需求将增加，养殖户补栏积极。大体重的仔猪尤其抢手。在猪市旺盛的带动下，其他畜产品市场也水涨船高，除水产料产量下降外，其他品种饲料均呈现出产销两旺的可喜局面。