植物病害管理战略决策的一个量化模型

 从植物病害流行结构分析入手,导出了以Logistic模式描述病害流行系统管理战略决策的数学模型:Z=In((1-X₀)/X₀)-r*t,并给出了相应的使用准则。初步试用的结果表明:它不仅十分简便和经济,而且是值得信赖的。     

Seasonal changes of azimuthal,radial, and tree-to-tree variations in sap flux affect stand transpiration estimates in a <Emphasis Type="Italic">Cryptomeria japonica</Emphasis> forest,central Taiwan

Stand transpiration (E) estimated using the sap flux methods is affected by the azimuthal, radial, and tree-to-tree variations of sap flux. Although several studies have examined the relative importance of the three variations in estimating E, the seasonality of the three variations remains unknown. In the current study, we attempted to clarify whether the relative importance of these three variations could show seasonal changes. Using sap flux data measured in a subtropical cloud forest from August 2010 to July 2011, we calculated the differences resulting from omitting the three variations in estimating E. The effects of the three variations in estimating E showed seasonality. The azimuthal and tree-to-tree variations were more pronounced during winter, whereas the radial variation was more pronounced during summer. However, the effect of tree-to-tree variation was consistently much larger than the other two variations throughout the study period. The tree-to-tree variation is more important in estimating E monthly, seasonally and annually than both the azimuthal and radial variations, although all three variations have shown seasonality. In addition, the sensor allocation for summer would be acceptable for the practical estimation of E if aiming at the long time scale.     

Nucleotide polymorphisms in the waxy gene of NaN3-induced waxy rice mutants

Spontaneous and induced waxy phenotype, associated with endosperm containing little or no amylose, has been recognized in rice (Oryza sativa L.). Mutation of a dominant gene Wx into a recessive gene wx, which causes the inactivation or absence of granule bound starch synthase, is believed to be responsible for the change in endosperm starch leading to the waxy grain. In the present study, the nucleotide polymorphism in the Wx gene of rice genotype Tainung 67 (wild type) and its 35 NaN₃-induced wx mutants were examined. Iodine staining confirmed that all the mutants had waxy grain trait. The G-to-T single base substitution analysis indicated that the wild type genotype Tainung 67 and its waxy mutants carried Wx^b allele. Moreover, 23-bp duplication in exon 2 was detected in all the waxy mutants. Microsatellite polymorphism (CT)_n was also detectable on the Wx gene of the tested genotypes and mutants, with at least 5 classes of (CT)_n microsatellites identified at the Wx locus. Electrophoretic analyses also confirmed the observed nucleotide polymorphsim. Thus, nucleotide polymorphsim exist among NaN₃-induced waxy mutants in rice. However, only the 23-bp duplication in exon 2 may be used as a molecular marker to characterize waxy grain trait in rice genotypes.     

Effects of different management practices on Portulaca oleracea emergence in soyabean

Portulaca oleracea (common purslane) is a summer annual weed with wide geographic distribution and is problematic in many crops worldwide. Field experiments were conducted to determine the effects of different management practices on P. oleracea emergence in soyabean fields. Two tillage systems [conventional tillage (CT) and no‐till (NT)], three soyabean seeding rates (SR) (200 000, 300 000 and 400 000 seeds ha^?1) and three imazethapyr doses (0, 50, and 100 g a.i. ha^?1 applied pre‐emergence) were considered as experimental factors. Portulaca oleracea emergence was affected by management practices including tillage system, soyabean SR and imazethapyr dose. Conventional tillage required a thermal time (TT) of 195.95 and 221.30 d °C to reach 50% emergence in 2016 and 2017, respectively, while for NT, the respective TT requirements were 182.34 and 203.32 d °C. On increasing soyabean SR from 200 000 to 400 000 seeds ha^?1, the TT requirements for 50% emergence (T₅₀) of P. oleracea also increased. The T₅₀ at the herbicide dose of 100 g a.i. ha^?1 was 193.05 and 220.67 d °C in 2016 and 2017, respectively, while for the non‐herbicide treatment, the respective TT requirements were 165.98 and 202.94 d °C. From an integrated weed management perspective, a combination of CT with a SR of 400 000 seeds ha^?1 and a 100 g a.i. ha^?1 imazethapyr dose not only resulted in the lowest P. oleracea seedling density m^?2 but also caused the longest delay in the time to reach the T₅₀. Findings from our study may facilitate the development of effective P. oleracea management strategies.     

Induction of autophagy and apoptosis by the extract of Solanum nigrum Linn in HepG2 cells

Solanum nigrum L. (SN) has been used in traditional folk medicine to treat different cancers. It is also used as a hepatoprotective and anti-inflammatory agent. In this study, we demonstrated that the extract of SN (SNE) induced a strong cytotoxic effect toward HepG2 cells but much less to Chang liver and WRL-68 cells. The mechanisms of the cytotoxic effect were concentration-dependent. High doses of SNE (2 and 5 mg/mL) induced apoptotic cell death in HepG2 cells, as evidenced by increases in the expressions of p-JNK and Bax, mitochodrial release of cytochrome c, and caspase activation. On the other hand, cells treated with low concentrations of SNE (50-1000 microg/mL) revealed morphological and ultrastructural changes of autophagocytic death under electron microscopic observation. Furthermore, these cells showed increased levels of autophagic vacuoles and LC3-I and LC3-II proteins, specific markers of autophagy. The levels of Bcl-2 and Akt that have been implicated in the down-regulation of autophagy were decreased upon SNE treatment. Taken together, these findings indicate that SNE induced cell death in hepatoma cells via two distinct antineoplastic activities of SNE, the ability to induce apoptosis and autophagocytosis, therefore suggesting that it may provide leverage to treat liver cancer.     

颗粒分选速度与土体温度之关系

着重于从事同一堆积材料试体在不同温度下受振荡之上升速度的变化试验,采用粒径 3.9m m之百丽珠做为堆积材,底层堆积高度（H_d 分别为 4,8,12,18cm共 4种高度,于放置同粒径不同颜色之百丽珠做为追迹珠后,上层堆积厚度（H_u）为 2 cm,试体初始温度 t设定为 20,21,22,23,24,25,26,27,28,29,30,31℃等 12种温度一共 48种处理。每一处理 5重复试验得一追迹珠上升 2 cm所需时间（T）与试体温度（t）之关系式。     

Antibody production in Japanese eels, Anguilla japonica Temminck & Schlegel

Adult Japanese eels, Anguilla japonica Temminck & Schlegel, (200–250 g, 45–55 cm) were immunized by intramuscular injection with goat IgG. After 5 weeks, eel immunoglobulin (Ig) was purified using affinity chromatography. The purified eel Ig was used to immunize rabbits to produce anti-eel Ig antibody. The highest antibody ELISA value in eels was reached 3 weeks after initial immunization with goat IgG, and then gradually decreased. The antibody could still be detected at 140 days post-immunization. The optimal temperature for antibody production was 30°C. Freund's complete adjuvant and secondary immunization both increased antibody production in eels.