Release and activity of allelochemicals from allelopathic rice seedlings

3-Isopropyl-5-acetoxycyclohexene-2-one-1 (1), momilactone B (2), and 5,7,4'-trihydroxy-3',5'-dimethoxyflavone (3) were isolated and identified from an allelopathic rice accession PI312777. These three compounds at low concentrations could inhibit the growth of weeds Echinochloa crusgalli and Cyperus difformis associated with rice, especially mixtures of the compounds had stronger inhibitory activity than did individual compounds. Studies with hydroponic culture, continuous root exudates trapping system (CRETS), and direct resin adsorption methods showed that a total of 7.6 n moles 1, 2, and 3 were exuded from living roots of each seedling into the environment at 10 days after seedlings were transplanted. Furthermore, 1, 2, and 3 were found in the soil growing PI312777 seedlings at day 15 after seedlings emergence and reached a total of 39.5 microg/g soil at day 30. The results indicated that PI 312777 seedlings could release sufficient quantities of 1, 2, and 3 into the environment to act as allelochemicals inhibiting the growth of associated weeds. Investigations on the distribution of 1, 2, and 3 in PI 312777 plant, and its root exudates showed that the levels of 1, 2, and 3 were significantly higher in the shoots and root exudates than in the roots, and only trace 1 was observed in the roots. The results suggest that the roots of rice seedlings are not major site of synthesis or accumulation 1, 2, and 3, but a pathway for their release into the environment. The levels of 1, 2, and 3 in the root exudates were over 2-folds higher under direct resin adsorption than under hydroponic culture and CRETS, and hence, it is the preferred method to collect and identify active allelochemicals in rice exudates in future studies on rice allelopathy.     

浅圆仓充氮气调技术实仓应用探究

通过对浅圆仓采用不同方法进行充氮试验,探索最佳的浅圆仓充氮气调储粮工艺。试验结果表明,采用上充下排方式,先以设备允许的最大流量(流量165m~3/h,浓度95%)充入氮气,当仓内氮气浓度接近95%时,再逐步提高设备氮气浓度至99%(流量90m~3/h),此工艺相较其他方法充氮速率略高,能耗相对略低,仓内氮气分布均匀。     

保护性耕作条件下浅松作业的试验

浅松作业是保护性耕作中推广使用的一种表土作业方式，适用于旱作保护性耕作的播前作业，能够达到松土、除草、整地等目的，也可以用于中耕除草作业。试验结果表明，该作业有良好的除草作用。能显著地提高地表平整度，降低表土容积密度，对根荏榭盖和土壤水分影响不显著，基本上满足了保护性耕作对土壤的要求。     

基于流场数值模拟的高速磁力泵汽蚀性能研

为了改善高速磁力泵的抗汽蚀性能,在泵吸入口处设置导流栅.建立了吸入口设置导流栅和未设置导流栅的两种模型,采用Fluent软件对高速磁力泵内流场进行数值模拟,给出了各自内部压力分布以及粒子流动迹线图.通过两组数据的比较,得到了内部流场的主要特征,模拟表明高速磁力泵吸入口设置轴向可变位导流栅可以有效提高抗汽蚀性能.数值模拟与高速磁力泵真机的试验对比表明,两者几乎一致,为高速磁力泵的改进设计提供了一定的理论依据.     

北京典型边缘区25年来土壤有机质的时空变异特征

采用地统计学和GIS相结合的方法,研究了北京典型边缘区25 a来两个阶段(1981～2000年,2000～2006年)土壤有机质含量(SOM)的时空变异特征.研究结果表明:土地利用类型及土地利用方式的转换对土壤有机质含量产生重要影响.1981～2000年,荒草地向耕地的转换以及因灌溉设施的完善而引起水浇地面积的增加使得整个研究区域土壤有机质含量普遍增加,有机质含量平均值从9.64 g/kg增加到12.35 g/kg,增加了28%;在利益的驱使下,农户改变了土地利用方式,不再向种植粮食作物的土地追加投入,研究区域土壤有机质含量平均值由12.35 g/kg降低到11.46 g/kg(2000～2006年).空间上,1981年土壤有机质含量自北向南逐渐降低;与1981年相比,2000年的土壤有机质含量普遍增加一个级别,增加最多的区域分布在研究区域的中部,这些区域也是土地利用类型变化明显的地方,主要是荒草地向其他农用地的转换;与2000年相比,2006年研究区域内土壤有机质含量增加的只有3个地区,并且大部分增加不显著,增幅不到1g/kg.     

Transitioning from standard to minimum tillage: Trade-offs between soil organic matter stabilization, nitrous oxide emissions, and N availability in irrigated cropping systems

Few studies address nutrient cycling during the transition period (e.g., 1–4 years following conversion) from standard to some form of conservation tillage. This study compares the influence of minimum versus standard tillage on changes in soil nitrogen (N) stabilization, nitrous oxide (N₂O) emissions, short-term N cycling, and crop N use efficiency 1 year after tillage conversion in conventional (i.e., synthetic fertilizer-N only), low-input (i.e., alternating annual synthetic fertilizer- and cover crop-N), and organic (i.e., manure- and cover crop-N) irrigated, maize–tomato systems in California. To understand the mechanisms governing N cycling in these systems, we traced ¹⁵N-labeled fertilizer/cover crop into the maize grain, whole soil, and three soil fractions: macroaggregates (>250 μm), microaggregates (53–250 μm) and silt-and-clay (<53 μm). We found a cropping system effect on soil N_new (i.e., N derived from ¹⁵N-fertilizer or -¹⁵N-cover crop), with 173 kg N_new ha⁻¹ in the conventional system compared to 71.6 and 69.2 kg N_new ha⁻¹ in the low-input and organic systems, respectively. In the conventional system, more N_new was found in the microaggregate and silt-and-clay fractions, whereas, the N_new of the organic and low-input systems resided mainly in the macroaggregates. Even though no effect of tillage was found on soil aggregation, the minimum tillage systems showed greater soil fraction-N_new than the standard tillage systems, suggesting greater potential for N stabilization under minimum tillage. Grain-N_new was also higher in the minimum versus standard tillage systems. Nevertheless, minimum tillage led to the greatest N₂O emissions (39.5 g N₂O–N ha⁻¹ day⁻¹) from the conventional cropping system, where N turnover was already the fastest among the cropping systems. In contrast, minimum tillage combined with the low-input system (which received the least N ha⁻¹) produced intermediate N₂O emissions, soil N stabilization, and crop N use efficiency. Although total soil N did not change after 1 year of conversion from standard to minimum tillage, our use of stable isotopes permitted the early detection of interactive effects between tillage regimes and cropping systems that determine the trade-offs among N stabilization, N₂O emissions, and N availability.     

不同预处理方式对柳枝稷厌氧发酵性能的影响

以柳枝稷为原料,研究微波,蒸汽加热和NaOH预处理方法对柳枝稷的结晶度和纤维结构的影响,同时考察了预处理后的柳枝稷在中温时的厌氧发酵性能.结果显示:3种处理都可以降低原料的结晶度;微波和NaOH处理可以显著降低柳枝稷的中性洗涤纤维、酸性洗涤纤维、木质素等含量;NaOH处理会降低粗脂肪的含量;蒸汽加热处理和原料的累积产气量(28 486和28 686 mL)及产气率(453.60和456.79mL/g)接近,而NaOH预处理由于C/N较高、阳离子毒害作用和抑制物的作用使得厌氧发酵性能降低.可见预处理可以降低柳枝稷纤维含量,但是对厌氧发酵性能并无明显改善作用.     

不同硬化地面类型对城市悬铃木物质循环的影响

研究了城市不同硬化地面类型（水泥地面、水泥砖面和踏实土地面）对悬铃木物质循环、水分循环及营养元素（Ｎ,Ｐ,Ｋ）循环的影响。结果表明：自然降水在城市水泥地面,水泥砖面或坚实的地表上很容易形成较大的地表径流,其中以水泥地面最甚,达４８％;各类型地面对雨水的渗透能力有明显差异,对照（花坛土）最强,为８７６％,踏实土（７２８％）次之,水泥砖面较差（３３％）,水泥地面渗透量接近于零。城市土壤营养元素除Ｋ外,Ｎ,Ｐ含量以踏实土最高,分别为００７７７和００８６６,其次为水泥砖面（００６９９和００５８７）及水泥地面（００５６２和００６３１）;生产力排序为：踏实土＞水泥砖面＞水泥地面。树木对土壤养分的吸收量也呈现相似的趋势,表现为：吸收量越大,理论归还量越大,但由于实际归还量较小,则土壤亏损就较多。     

PCR技术在住肉孢子虫虫种鉴定上的应用

采用 RAPD 方法对水牛梭形住内孢子虫、待定种及黄牛枯氏住内孢子虫缓殖子基因组 DNA 进行了分析,待定种 PCR 产物的电泳带型与枯氏住肉孢子虫的差异较梭形住内孢子虫大,这与二者表型性状一致的研究结果不吻合.对3种包囊种特异性的 PCR 指纹进行了筛选,回收和纯化了3种包囊种特异性的 DNA 片段,待定种0.7kb 片段经α-~(32)PdATP 标记后用作探针,只与同源 DNA 杂交,不与其它包囊、宿主细胞和艾美耳球虫杂交,用 pGEM-T Easy Vector 对该片段克隆成功,并测出其全长序列,为进一步设计特异引物建立标准 PCR 诊断方法和用作核酸探针奠定了基础。     

粗山羊草(Aegilops tauschii)遗传多样性的研究进展

概述了粗山羊草（Ａｅ．ｔａｕｓｃｈｉｉ（Ｃｏｓｓ．）Ｓｃｈｍａｌ．）的类别和地理分布以及其在山羊草属（Ａｅｇｉｌｏｐｓ）分类中的地位。并从个体、细胞、生化和分子水平上对粗山羊草遗传多样性的研究进展作了系统的综述。这 结果对进一步研究和利用业山羊草以及其它小麦近缘植物具有重要意义。