农业机械的绿色设计

本文提出了农业机械进行绿色设计的观点,阐述和分析了农业机械绿色设计的综合评价体系的评价准则和评价指标的构成与内容,讨论了农业机械绿色设计的内容。     

杂交水稻及其花粉植株后代某些生理生化特性比较研究

杂交水稻(Oryza sative)汕优桂34F_1的三个花培品系89 AH-97,89 AH-167和88AHW-16叶绿素含量在分蘖盛期明显低于亲本汕优桂34F_1,在孕穗期则差异不大。但三个花培品系的净光合强度在分蘖盛期与汕优桂34F_1无明显差异,在孕穗期则显著地高于汕优桂34F_1。各品系的乙醇酸氧化酶活性均无明显差别。同时,三个花培品系的硝酸还原酶活性和核酸量均极显著高于汕优桂34F_1,其可溶性蛋白质含量在分蘖盛期和孕穗期亦均显著地高于汕优桂34F_1。经4℃低温处理后,三个花培品系脯氨酸含量增加率极显著地低于汕优桂34F_1,其超氧物歧化酶(SOD)活性下降率则极显著地高于汕优桂34F_1。而三个花培品系的酯酶和SOD等同工酶谱无质的差别,只有某些酶带的活性与汕优桂34F_1有不同程度的差异。以上结果表明,通过花药(粉)培养所得植株后代可相对地将杂交水稻亲本某些优良性状“稳定”下来。     

试析两种中文检索刊物的查全、查准率

在编辑《中国牦牛文献题录》过程中，检索了《中文科技资料目录》和《全国报刊索引》，以及进行了相应的联机检索。比较了两种中文核心期刊在检索时的查全率和查准率，从其变动中分析了影响查全、查准率的因素，以期从根本上改进中文检索工具的检索效率。     

龙蟠村迟熟椪柑疫腐病调查及防治方法

蒙山县西河镇龙蟠村15户果农,在2001年春种下迟熟椪柑15 hm2,在果农精心护理下,果树长势良好,2003年开始挂果.     

蜂花粉系列研究

系统研究了蜂花粉营养成分及生物学功能，说明花粉属于完全营养食品并具有多种生物学作用。     

A dominant antigenic epitope on SARS-CoV spike protein identified by an avian single-chain variable fragment (scFv)-expressing phage

Severe acute respiratory syndrome (SARS) is a newly emergent human disease, which requires rapid diagnosis and effective therapy. Among antibody sources, immunoglobulin Y (IgY) is the major antibody found in chicken eggs and can be used as an alternative to mammalian antibodies normally used in research and immunotherapy. In this study, phage-expressing chicken monoclonal scFv antibody was chosen and characterized with phage display antibody technology. Truncated fragments of SARS-CoV spike protein were cloned in pET-21 vector and expressed in BL-21 Escherichia coli (E. coli) cells. After purification, the purity of these recombinant spike proteins was examined on SDS-PAGE and their identity verified with Western blot analysis using anti-his antibodies and sera from convalescent stage SARS-CoV-infected patients. Using these bacteria-derived proteins to immunize chickens, it was found that polyclonal IgY antibodies in the egg yolk and sera were highly reactive to the immunogens, as shown by Western blot and immunocytochemical staining analysis. A phage displaying scFv library was also established from spleen B cells of immunized chicken with 5 x 10(7) clones. After four panning cycles, the eluted phage titer showed a 10-fold increase. In sequence analysis with chicken germline gene, five phage clones reacted, with large dissimilarities of between 31 and 62%, in the complementarity-determining regions, one dominant phage 4S1 had strong binding to fragment Se-e, located between amino acid residues 456-650 of the spike protein and this particular phage had significantly strong binding to SARS-CoV-infected Vero E6 cells. Based on the results, we conclude that generating specific scFv-expressing phage binders with the phage display system can be successfully achieved and that this knowledge can be applied in clinical or academic research.     

疏果与套袋对橘柚果实品质的影响

通过对橘柚进行不同疏果与不同套袋方法处理,试验结果表明,疏果处理显著提高橘柚单果重和可溶性固形物含量,提高优质果比例,有利于实现丰产稳产;不同套袋处理的果实均比不套袋感观指标变化明显,其中优质果所占比例明显提高,套袋后的果实可溶性固形物含量降低比较明显。综合处理效果以适量疏果(叶果比保持在30∶1)加用双层遮光纸果袋最为理想。     

Effects of Vegetation Stems on Hydraulics of Overland Flow Under Varying Water Discharges

Vegetation plays an important role in soil erosion control, but few studies have been performed to quantify the effects of vegetation stems on hydraulics of overland flow. Laboratory flume experiments were conducted to investigate the potential effects of vegetation stems on Reynolds number, Froude number, flow velocity and hydraulic resistance of silt‐laden overland flow. Cylinders with diameter D of 2·0, 3·2 and 4·0 × 10⁻² m were glued onto the flume bed to simulate the vegetation stems, and a bare slope was used as control. The flow discharge varied from 0·5 to 1·5 × 10⁻³ m³ s⁻¹ and slope gradient was 9°. Results showed that Reynolds number on vegetated slope was significantly higher than that on bare slope because of the effect of vegetation stems on effective flow width. All the flows were supercritical flow, but Froude number decreased as D increased, implying a decrease in runoff ability to carry sediment. The mean flow velocity also decreased with D, while the velocity profile became steeper, and no significant differences were found in surface flow velocities among longitudinal sections on all slopes. Darcy–Weisbach friction coefficient increased with D, implying that the energy consumption of overland flow on hydraulic resistance increased. Reynolds number was not a unique predictor of hydraulic roughness on vegetated slopes. The total resistance on vegetated slopes was partitioned into grain resistance and vegetation resistance, and vegetation resistance accounted for almost 80% of the total resistance and was the dominant roughness element. Further studies are needed to extend and apply the insights obtained under controlled conditions to actual overland flow conditions. Copyright © 2015 John Wiley & Sons, Ltd.     

Simulation Study of the Impact of Permanent Groundcover on Soil and Water Changes in Jujube Orchards on Sloping Ground

Land degradation is recognized as a major environmental problem in rainfed fruit orchards on the Chinese Loess Plateau. Six treatments were used to investigate surface runoff and soil moisture by means of simulated rainfall experiments: (i) a control (clean cultivation) (CC); (ii) strip cock's foot (Dactylis glomerata L.) cover (SCF); (iii) strip crown vetch (Coronilla varia L.) cover (SCV); (iv) strip bird's foot trefoil (Lotus corniculatus L.) cover (SBF); (v) strip white clover (Trifolium repens L.) cover (SWC); and (vi) complete white clover cover (WCC). The time to runoff was significantly longer under WCC than under other treatments (p < 0·05). The total runoff volume and sediment yield were significantly greater under CC than under the vegetation cover treatments (p < 0·05). The mean infiltration rate under WCC and CC was the largest and lowest and differed significantly from that under other treatments (p < 0·05). The change of soil water storage was the largest under WCC and the least under CC. The soil moisture was significantly greater under SCF than under other treatments (p < 0·05). Treatment SCF seemed to be the best groundcover for rainfed sloping jujube orchards on the Chinese Loess Plateau. Copyright © 2014 John Wiley & Sons, Ltd.