Fusariosis in rubber tree: pathogenic,morphological, and molecular characterization of the causal agent

Fusariosis, one of the phytosanitary problems found in rubber producing areas in the northwest of the state of São Paulo, is a disease that affects the bark of the adult plants, affecting the exploitation of latex. The typical symptoms appear as cracks in the bark that expand from the rootstock towards the tapping panel, causing a drying of the latex flux in the injured region, impeding latex tapping. Due to the recent incidence of this disease in rubber plantations, the goal of this study was to characterize the Fusarium associated with symptomatic rubber tissue in three different locations in the state of São Paulo. In order to identify Fusarium species, pathogenicity, morphological, cultural and molecular studies were carried out. A total of 51 isolates were obtained and separated into three groups based on macroconidium morphology, presence or absence of sporodochia, types of chlamydospores, formation of phialides and conidiogenesis of microconidia and mesoconidia, mycelial growth rate and coloring of the colonies. These groups were corroborated using DNA sequence information for five different genetic loci, and were subsequently recognized as Fusarium oxysporum, F. incarnatum and F. decemcellulare. Our results further showed that all 51 of the Fusarium isolates recovered were pathogenic to rubber tree seedlings of RRIM 600 standard clone. This study also reports for the presence of F. oxysporum and F. incarnatum in rubber plantations in the state of São Paulo and in Brazil.

     

Development of a clinical severity index for dogs with acute pancreatitis

Objective-To establish a clinical severity index that correlates severity of body system abnormalities with outcome in dogs with acute pancreatitis (AP) and determine the usefulness of serum C-reactive protein (C-RP) concentration as an objective measure of AP severity. Design-Retrospective cohort study. Animals-61 client-owned dogs with ultrasonographically or histologically confirmed AP. Procedures-Medical records of AP-affected dogs were reviewed, and signalment, physical examination findings, clinicopathologic data, and outcome (death or discharge from the hospital) were evaluated. The correlation of specific abnormalities in endocrine, hepatic, renal, hematopoietic, cardiovascular, and respiratory systems; local pancreatic complications; and intestinal integrity were evaluated, and a clinical severity index was developed for AP in dogs. The severity index score was compared with outcome and, for 12 dogs, with serum C-RP concentration. Results-The clinical severity index had a good correlation with outcome and interval from hospital admission until end point (days until outcome), but there was no difference in days until outcome between survivors and nonsurvivors. All 12 dogs evaluated had high serum C-RP concentration, but this variable was not related to outcome; however, within a 2-day period after onset of clinical signs, serum C-RP concentration in survivors and nonsurvivors differed significantly. Conclusions and Clinical Relevance-Among AP-affected dogs, the clinical severity index may be useful for treatment comparisons and prediction of intensive management requirements. Serum C-RP concentration was best related to AP severity within a 2-day period after onset of clinical signs, but daily measurement may be more useful for monitoring progress.     

Models for predicting protein requirements for meat quail

Three experiments were carried out to determine the crude protein requirements for maintenance (CPm) and weight gain (CPg) of meat quail and to develop protein‐requirement prediction models. Experiment 1 was conducted to determine CPm by the nitrogen‐balance technique. The regression of nitrogen balance on nitrogen intake revealed a CPm requirement of 2.94 g/kg^0.75/day. Experiment 2 was aimed at determining CPm by the comparative‐slaughter technique. Retained nitrogen (RN) and nitrogen intake (NI) were quantified considering the metabolic weight of the birds. The linear regression of RN on NI provided a CPm estimate of 6.63 g/kg^0.75/day. Experiment 3 was conducted to determine CPg. The regression of body nitrogen from the carcasses on fasted body weight revealed CPg estimates of 407.68 (0–7 days), 501.76 (8–14 days), 470.40 (0–14 days), 517.44 (15–21 days), 627.20 (22–28 days), 423.36 (29–35 days), and 517.44 mg/g (15–35 days). The protein‐requirement prediction models developed for meat quail aged 0–7, 8–14, 0–14, 15–21, 22–28, 29–35, and 15–35 days were CP = 2.94.W^0.75 + 0.408.G; CP = 2.94.W^0.75 + 0.502.G; CP = 2.94.W^0.75 + 0.470.G; CP = 2.94.W^0.75 + 0,517.G; CP = 2.94.W^0.75 + 0.627.G; CP = 2.94.W^0.75 + 0.423.G; CP = 2.94.W^0.75 + 0.517.G, respectively, where: W^0.75 =  metabolic weight (kg), and G =  daily weight gain (g).     

Diffuse leptomeningeal histiocytic sarcoma in the cerebrospinal fluid of 2 dogs

Two adult male castrated dogs were evaluated for progressive paraparesis and ataxia. Neurologic examination showed severe ataxia, delayed proprioceptive placement in the pelvic limbs, pain upon palpation of the lumbar spine as well as facial paresis in one dog, and decreased withdrawal reflex of the pelvic limbs in the other dog. Magnetic resonance imaging (MRI) in both dogs showed diffuse meningeal and intramedullary lesions. However, no evidence of a mass was found. Biopsies could not be performed safely due to the location of the lesions. Cerebrospinal fluid (CSF) examination revealed an inflammatory pleocytosis associated with increased protein concentration and numerous large atypical round cells, often multinucleated. Nuclear fragmentation, micronuclei, and rare atypical mitoses were observed. Immunocytochemistry revealed CD1⁺ and CD11c⁺ staining, which, in concert with the morphology confirmed the diagnosis of histiocytic sarcoma (HS). Euthanasia was elected due to poor prognosis. Histopathologic examination showed diffuse spinal and meningeal infiltration with CD18⁺ neoplastic cells, without any evidence of mass formation, which completed the diagnosis of diffuse leptomeningeal HS involving the brain and the spinal cord. Canine central nervous system (CNS) HS has been seldom reported in the literature, with only isolated cases identified on CSF cytology. The cases reported here are remarkable in describing a diffuse CNS leptomeningeal HS associated with neoplastic cells in the CSF of dogs without a tumor mass. These cases emphasize the potential critical importance of CSF analysis in providing an antemortem diagnosis of neoplasia in neurologic patients.     

Humoral immune responses to Mycoplasma hyopneumoniae in sows and offspring following an outbreak of mycoplasmosis

Previously healthy sows, seropositive to Mycoplasma hyopneumoniae, developed clinical signs of mycoplasmosis, as well as increasing amounts of antibodies to M. hyopneumoniae during an outbreak of the disease in a herd. During the early phase of the outbreak, young piglets (2 weeks) with maternal antibodies remained healthy while older seronegative piglets (4–7 weeks) developed the disease. The duration of the maternal antibodies to M. hyopneumoniae varied between litters and was related to the amount of antibodies in the serum of the dam. In sows, the level of serum antibodies decreased continuously from 4 weeks ante partum to partus, and the level of antibodies in the whey of colostrum was comparable to that in serum 4 weeks ante partum. After loss of maternal antibodies to M. hyopneumoniae, seropositive animals were not found among piglets younger than 9 weeks. Therefore peripheral blood mononuclear cells (PBMC) were collected from various age categories of piglets in order to measure the ability to produce antibodies to M. hyopneumoniae in vitro. PBMC obtained from piglets aged 1 and 3 weeks produced few antibodies to M. hyopneumoniae. Significantly higher levels of antibodies to M. hyopneumoniae were produced by PBMC obtained from pigs aged 5–9 weeks. Thus, the ability of PBMC to produce antibodies to M. hyopneumoniae in vitro seemed to be age-dependent.     

Effects of annual grass senescence on NH4 and N-glycine uptake by blue oak (Quercus douglasii) seedlings and soil microorganisms in California oak woodland

Annual grasses are stronger competitors for available soil N than blue oak seedlings and soil microorganisms. However, little is known about the dynamics of N competition during annual grass senescence. We conducted a field experiment in a California oak woodland to study effects of annual grass senescence on N uptake by grasses, blue oak seedlings and soil microorganisms. Labeled N was applied at the beginning of April, May and of June in the form of ¹⁵NH₄⁺ or ¹⁵N-glycine. Plants and soils were harvested after 5 days (¹⁵NH₄⁺ and ¹⁵N-glycine treatments) and after 26 days (¹⁵NH₄⁺ treatment only). We evaluated effects of N form, season and labeling period on N competition among oak seedlings, annual grasses and soil microorganisms. N forms did not affect competition among grasses, oak seedlings and soil microorganisms, but more ¹⁵N was incorporated into the soil organic N pool in the ¹⁵N-glycine treatments than in the ¹⁵NH₄⁺ treatments. There were no seasonal (May vs June) effects on ¹⁵N recovery in blue oak seedlings and soil microorganisms. Plant samples from April harvest were lost. In June, when grasses were senescing, more ¹⁵N was found in the soil inorganic pool than in May. Extremely dry soils in June may have limited inorganic N availability to oak seedlings and soil microorganisms. After 26-day labeling period, ¹⁵N recovery in blue oak seedlings and the soil organic N pool significantly increased, while ¹⁵N recovery in both the soil microbial and inorganic N pools decreased compared to the 5-day labeling period. Although blue oak seedling biomass changed little from early May to late June, N concentrations in oak roots increased 53%. In contrast, annual grass biomass peaked in May, and then decreased rapidly. Our results suggest that blue oak seedlings and annual grasses have different temporal competitive abilities. Blue oak seedlings appear to have a long-term strategy for N competition. Blue oaks take up N slowly but steadily, increasing N uptake from 5 to 26 days. This extended time period has a greater positive effect on N uptake than does reduced grass uptake caused by senescence.     

Temperature effects on bioremediation of PAHs and PCP contaminated south Louisiana soils: A laboratory mesocosm study

Goal, Scope and Background  Temperature and soil moisture content are important environmental variables in bioremediation technologies. Optimizing these variables in-situ would enhance and maintain remediation of hazardous wastes during cold winter seasons or in cold regions and may lead to reduced maintenance and/or cost. The effect of elevated temperature and soil moisture on bioremediation efficiency was investigated using a laboratory mesocosm approach. Selected polycyclic aromatic hydrocarbons (PAHs) and phenols degradation in contaminated flooded soils, commonly found in Superfund sites situated in coastal plains sediments/soils, were evaluated in the mesocosms. Material and Methods  Four laboratory mesocosm treatments in triplicate simulating in-situ bioremediation of contaminated site soils using an immobilized microbe bioreactor system, i.e., bioplug, were established to evaluate temperature effects. Elevated temperature treatments of site soils with and without contaminant-specific microorganisms were established at a temperature of 42±2°C. Similarly, treatment of site soils with and without contaminant-specific microorganisms were established at an ambient temperature of 21±1°C. Composite samples were analyzed for selected PAHs and chlorinated phenols to determine rates of mineralization and overall remediation efficiency for different temperature regimes. Results  Mesocosm studies indicated that the high temperature inoculated treatment demonstrated a significant reduction in mean total PAHs and total phenols with a kinetic rate (KR) of 76±13 ng g⁻¹ d⁻¹ in 49 days (approximately 84% reduction; p<0.01) The KR for low temperature inoculated treatment was 54±1 ng g⁻¹ d⁻¹ in 49 days (approximately 66% reduction; p<0.01). High temperature non-inoculated mesocosms exhibited significant mineralization of all constituents with KR of 15±6 ng g⁻¹ d⁻¹ (approximately 65% reduction; p<0.01) in 49d compared to 54% reduction for low temperature non-inoculated treatment with KR of 12±3 ng g⁻¹ d⁻¹ (p=0.1794). Phenol compounds in inoculated treatments were also significantly reduced (65%, p<0.01) at elevated temperatures compared to ambient (52%, p<0.01). Discussion  Increased bioavailability and desorption were noted for elevated temperature and moisture in the soil laboratory mesocosms simulating a field in situ remediation protocol. This protocol employing the application of immobilized microflora indicated that in situ systems provide an economical advantage if optimal elevated temperature and moisture are controlled properly. Results also suggested that temperature and moisture optimization needs to be combined with efficient nutrients delivery systems for impacted soils/sediments. Conclusions  The study demonstrated that temperature and soil moisture contents are important factors in the success of in-situ bioremediation techniques at hazardous waste sites situated in a coastal zone. Kinetic rates were significantly enhanced to remediate known recalcitrant compounds (PAHs and phenols) in aged soil. Recommendations and Perspectives  The placement of a preferred microbial consortia such as an immobilized microbial population in an entrained bioreactor, i.e., bioplug, can significantly reduce constituents of concern in a timely manner for contaminated soils/sediments. However, frequent monitoring of the soil temperature, moisture content, nutrient level, and dissolved oxygen is necessary to achieve predictable kinetic rates of mineralization. ESS-Submission Editor: Dr. Teresa Cutright (tcutright@uakron.edu)     

Adjusting P-K Fertilization and Liming Strategies to Enhance Yield of Cherry Tomato Plants Grown on an Oxisol

ABSTRACT

Although cherry-tomato is becoming more popular in the last years, there are no specific recommendations for P and K fertilization or liming strategies for this specific variety. Our aim was to assess rates of P and K and liming strategies in order to improve the yield of cherry-tomato grown on an Oxisol with a limited P-availability. Two pot experiments were developed. In the first-one, a two-factor factorial design was developed with 4 P (first-factor) and 4 K rates (second-factor), being these rates the 0%, 50%, 100%, and 200% of the recommended fertilization for tomato in South-Brazil. In the second experiment, the soil pH (4.3) was adjusted to 5.5, 6.0, and 6.5 with limestone. Soil P-availability, plant growth, diameter, number of fruits and cherry tomato yields were increased with the P addition, while a non-significant effect was observed after adding K. The highest yield was produced with 50% of the suggested amount of P for tomato in comparison with the non-fertilized plants (yield increase of 674%), while higher P rates (100% and 200%) produced a lower yield increase. The significant effect of soil liming was observed on increase of the flower bunches number when the soil pH was amended to 6.5. Cherry-tomato requirements seem to be different from the recommended for other varieties. Our results indicate that higher yields were achieved with a lower amount of chemical inputs than the recommended for tomato in general, stressing the importance of P for tomato cherry production over the other factors assessed here.     

Experimental platforms for the investigation of spatiotemporal patterns in the rhizosphere—Laboratory and field scale

The numerous feedback loops between roots, microorganisms, soil chemical and physical properties, and environmental variables result in spatial parameter patterns which are highly dynamic in time. In order to improve our understanding of the related rhizosphere processes and their relevance at the soil–plant system scale, experimental platforms are required. Those platforms should enable (1) to relate small scale observations (nm to dm) to system behaviour, (2) the integration of physical, chemical and biological sampling approaches within the same experiment, and (3) sampling at different time points during the life cycle of the system in question. Here we describe what requirements have to be met and to what extent this has been achieved in practice by the experimental platforms which were set up within the framework of DFG priority programme 2089 “Rhizosphere Spatiotemporal Organisation—a key to rhizosphere functions”. It is discussed to what extent theoretical considerations could be accommodated, in particular for the comparison across scales, i.e., from laboratory to field scale. The latter scale is of utmost importance to overcome the trade‐off between fraction of life cycle covered and the avoidance of unrealistic root length densities.