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71.
在调查研究的基础上,分析导致东北平原北部的望奎县耕地黑土退化的相关因素,并相应地提出了保持土地资源生产力、实现可持续发展的治理策略。 相似文献
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主要介绍CAN(Controller Area Network)总线在电动车门和车灯两个智能节点上的应用。本研究方法是对其选定的节点进行软硬件设计以及协议的制定,最后实现其模拟仿真。 相似文献
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LVQ学习矢量量化神经网络模型在旱涝预测中的应用 总被引:1,自引:0,他引:1
旱涝灾害的预测对预防灾害和保护环境有着重要的作用。运用神经网络中LVQ学习矢量量化神经网络建立了旱涝预测模型,模型输入层神经元数目为3,输出层神经元数目为1,隐含层神经元数目采用实验法,最终确定为7。预测结果表明:该方法与传统的预测方法及BP神经网络相比,具有更强的容错性和鲁棒性,预测精度较高且计算简单等优点。 相似文献
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AIM:To investigate the inhibitory effect of thioredoxin 1 (Trx-1) over-expression on oxidative stress injury in 1-methyl-4-phenylpyridinium (MPP+)-induced rat pheochromocytoma PC12 cells by regulating NF-κB signaling pathway.METHODS:The PC12 cells were damaged by treatment with MPP+ at 1, 3 and 5 mmol/L, and the optimal concentration of 3 mmol/L was selected. The cell viability was measured by MTT assay. The oxidative stress indexes lactate dehydrogenase (LDH) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in the cell culture supernatant were detected, and the protein expression of Trx-1 was determined by Western blot. Lentiviral infection with Ad-Trx-1-GFP sequence was used to establish a model of MPP+-treated PC12 cells with Trx-1 over-expression. The effects of Trx-1 over-expression on the cell viability, oxidative stress responses and NF-κB signaling pathway were determined by MTT assay, commercial kits and Western blot. The effects of phorbol 12-myristate 13-acetate (PMA), an activator of NF-κB signaling pathway, on the viability and oxidative stress of PC12 cells were observed. The NF-κB signaling pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used in MPP+-treated PC12 cells with Trx-1 over-expression, and the cell viability and oxidative stress responses were measured. RESULTS:The viability of PC12 cells, SOD activity in the supernatant and protein expression of Trx-1 were decreased, while LDH activity and MDA content in the supernatant were increased significantly by treatment with MPP+ at 1, 3 and 5 mmol/L. The effect of MPP+ at 3 mmol/L and 5 mmol/L was significantly greater than that at 1 mmol/L (P<0.05), and no significant difference between 3 mmol/L and 5 mmol/L was observed (P>0.05). The inhibitory effect of MPP+ on the viability of PC12 cells, and the oxidative stress injury and activation of NF-κB signaling pathway induced by MPP+ were significantly attenuated by over-expression of Trx-1. The inhibitory effect of MPP+ on the viability of PC12 cells and the oxidative stress injury induced by MPP+ were promoted by the activation of NF-κB signaling pathway, while the protective effects of Trx-1 over-expression on the MPP+-treated PC12 cells were enhanced by the inhibition of NF-κB signaling pathway. CONCLUSION:Over-expression of Trx-1 protects MPP+-treated PC12 cells from oxidative stress injury by regulating NF-κB signaling pathway. 相似文献
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AIM: To investigate the expression and roles of family with sequence similarity 3, member C (FAM3C) in oral squamous-cell carcinoma cells. METHODS: The mRNA and protein expression levels of FAM3C in dysplastic oral keratinocyte (DOK) and oral squamous-cell carcinoma WSU-HN6 cells were detected by RT-qPCR and Western blot. The WSU-HN6 cells were treated with siFAM3C or FAM3C antibody. After 24, 48 and 72 h, the viability of WSU-HN6 cells was measured by CCK-8 assay, and the activation of protein kinase B (Akt) was detected by Western blot. Adenovirus was used to mediate over-expression of FAM3C in the DOK cells. The DOK cell viability was measured by CCK-8 assay after adenovirus infection for 24, 48 and 72 h, and the activation of Akt was detected by Western blot. RESULTS: Compared with the DOK cells, the mRNA and protein levels of FAM3C were significantly increased in the WSU-HN6 cells (P<0.05). The viability of WSU-HN6 cells transfected with siFAM3C was significantly inhibited at 48 h and 72 h (P<0.05). siFAM3C treatment inhibited the activation of Akt (P<0.05). FAM3C antibody treatment also suppressed the viability of the WSU-HN6 cells at 48 h and 72 h and the activation of Akt (P<0.05). Over-expression of FAM3C in the DOK cells promoted the cell viability at 48 h and 72 h and activated Akt (P<0.05). CONCLUSION: FAM3C might promote oral squamous-cell carcinoma cell growth by activating Akt. 相似文献
77.
为探究紫花苜蓿不同种植布局对苹果园害虫及天敌的影响,在山东省沂水市苹果园设置了紫花苜蓿逐行、隔1行和隔2行种植的布局方式,以自然生草区为对照,系统调查了4种生草方式苹果园果树上和生草上害虫及天敌的发生动态。结果表明:害虫的调查总量,紫花苜蓿逐行种植区最少,比隔1行种植区少20.29%,比隔2行种植区少48.53%,比自然生草区少49.91%;苹果树上天敌的调查总量,紫花苜蓿隔1行种植区最多,是逐行种植区的1.13倍、隔2行种植区的1.29倍、自然生草区的1.78倍;生草上天敌的调查总量,紫花苜蓿逐行种植区最多,是隔1行种植区的1.05倍、隔2行种植区的1.19倍、自然生草区的1.66倍;益害比方面,紫花苜蓿逐行种植区和隔1行种植区的益害比明显高于其他生草区。综合防治效果和种植成本,紫花苜蓿隔1行种植的布局方式更合理。 相似文献
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