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AIM and METHODS:To analysis the factor that involved in renal carcinogenesis, we used the bait gene AK001518 to screen GenBank. To understand the relationship between cell cycle related gene(CCRG) and p15, we did RT-PCR and Northern Blot experiments. Then we examined CCRG expression level in renal carcinogenesis. RESULTS:Gained a function unknown gene CCRG that was 67% a mino acid identical with the gene AK001518 that was regulated by p15. It was shown that the CCRG mRNA was dramatically decreased when p15 gene was over-expressed. CCRG expression level was much higher in tumor tissues and cells than normal tissues and cells. CONCLUSION:The novel gene CCRG expressed highly in the renal carcinoma, which might play a significant role in the renal carcinogenesis. 相似文献
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城市绿地系统对经济发展提升作用的机制——与大连市实证研究 总被引:6,自引:2,他引:6
城市绿地系统具有生态、经济、社会等多重属性 ,在城市复合系统中占有特殊的位置 ,对城市经济发展亦具有直接、间接的提升作用 :( 1 )改善城市环境质量 ,创造环境优势 ,促进城市地价及布局其间的各种经济成分增值 ;集聚外来资金和高新产业发展 ,带动整个城市产业结构优化升级。( 2 )绿地建设作为城市经济产业的有机组成部分 ,形成环境经济产业链 ,拉动其相关经济产业的发展 ,促进经济增长。 ( 3 )提高城市知名度 ,促进城市旅游业发展 ,集聚高素质人才 ,促进城市经济发展 ,本文以北方海滨城市大连为例 ,在定性分析绿地经济提升作用机制的基础上 ,建立计量经济模型 ,定量分析城市绿地系统的经济提升作用 相似文献
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AIM: To investigate expression of CD44s in lung cancer and it's clinical significance. METHODS: A total of 117 primary lung cancer from patients were examined for CD44s expression by immunohistochemical staining. RESULTS: CD44s mostly expressed in non-small cell lung cancer (NSCLC) but not in small ecll lung cancer (SCLC), and squamous cell carcinoma(SCC) showed much stronger expression of CD44s than adenocarcinoma(ADC)(P<0.05). In comparison of the lung cancer with/ without lymph node metastasis, the latter showed stronger expression of CD44s(P<0.01). According to TNM, there was a distinct statistic difference between early stage and advanced stage(P<0.05). CONCLUSION: CD44s might be a better indicant in histological classification of lung cancer, lymph node metastasis, clinical stage and prognosis. 相似文献
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AIM: To observe effects of homocysteine and antagonized effects of taurine on electronic leakage and free radical production in myocardial mitochondria. METHODS: Myocardial mitochondria of rat heart was isolated, and was broken by supersonic wave to prepare submitochondria. Recombinant of succinic acid cytochrome c reductase was prepared with mitochondria of porcine heart. They were co-incubated with homocysteine and/or taurine with various concentration. The H2O2 and O2- were determined by chemiluminescence methods. The taurine transporter of heart mitochondria and its propert, and effects of homocysteine on its function were studied with glass filter. RESULTS: Homocysteine stimulated oxygen free radical production in heart mitochondria, submitochondria, and succinic acid cytochrome c in a concentration-dependent manner. Although taurine itself did not affect oxygen free radical production, taurine did inhibit oxygen free radical production in mitochondria, submitochondria and succinic acid cytochrome c in a concentration-dependent manner. Taurine transporters of Na+-dependent were existed in mitochondria membrane. Homocysteine inhibited taurine transtport in mitochondria in a concentration-dependent manner. CONCLUSIONS: Taurine inhibited electronic leakage and oxygen free radical production induced by homocysteine in electron transport chain. There were taurine transporters in mitochondria membrane, and transport functions of taurine transporter were inhibited by homocysteine. 相似文献
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AIM: To observe the change of insulin receptor in rabbit kidney with acute ischemic-reperfusion injury. METHODS: 15 Japanese white rabbits were allocated randomly into control group, ischemic-reperfusion group(IR group). IR group received clamping for 1 h followed by 2 h or 48 h of reperfusion. At 2 h or 48 h after reperfusion, glucose and insulin in serum were determined. Insulin receptor in renal tissue was analyzed by radioligand binging assay(BAD). RESULTS: The level of serum glucose increased after 2 h reperfusion in 2 groups, but in IR group the value increased much more higher than those in control groups(P<0.05). Plasma insulin of IR group was significantly higher than that in control after 2 h reperfusion(P<0.05). Scatchard analysis of data resulted in curvilinear profiles, indicating that there are two classes of receptors with different affinity or the presence of a single class of receptors with a negative cooperative hormone-receptor interaction. Data analyzed for a two-site model showed that the values of Bmax1(high affinity site), Bmax2(low affinity site) and Kd1, Kd2 were significantly lower than that of control (P<0.05) after 2 h perfusion. 48 h after IR there was no difference of Bmax1, Bmax2, and Kd1 between 2 groups,but Kd2 of IR group was higher than that of control (P<0.05). CONCLUSIONS: The results indicate that the effect of intrinsic insulin decreases in the progress of the renal ischemic-reperfusion. The resulting high serum glucose may aggravate renal injury in the progress of ischemic-reperfusion. 相似文献
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三种优良禾本科牧草与本地品种生长性状对比分析 总被引:1,自引:0,他引:1
分析了三种引进优良禾本科牧草与一种当地禾本科牧草的生长性状,结果表明:三种引进禾本科牧草的鲜重均比当地禾本科优良牧草垂穗披碱草高,种植第三年引进牧草品种的鲜草产量为无芒雀麦19316kg/hm^2,达乌里披碱草16457kg/hm^2,猫尾草15754kg/hm^2。引进的三种牧草品种表现出较好的抗逆性,推广价值较高,适宜在高寒地区种植。 相似文献
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基于研究区多年降水特征,设置高频低量、中频中量和低频高量3种降水格局,采用凋落物分解袋法,对科尔沁沙地3种常见一年生草本植物一个生长季地上和地下凋落物分解特征进行了研究。结果表明,降水格局和凋落物埋深均是影响分解率的关键因素。其中地表凋落物的分解率显著低于地下凋落物,随埋深增加地下凋落物的分解率有增加的趋势,10~20 cm层狗尾草地下凋落物分解率显著高于0~10 cm层(P<0.01)。低频高量降水状况下地上凋落物的分解率显著降低(P<0.01)。降水格局与凋落物埋深存在显著的耦合关系(F=3.60, P=0.008),随土壤深度增加,降水格局对凋落物分解率的影响减少,且物种间存在差异性,不同降水格局下狗尾草和黄蒿地上凋落物和0~10 cm埋深地下凋落物分解率均存在显著差异性(P<0.05),而尖头叶藜仅地上凋落物在不同降水格局下的分解率表现出显著差异性(F=48.42, P<0.001)。 相似文献