miRNA-7 inhibits proliferation of human nasopharyngeal 5-8F carcinoma cells via EGFR/PI3K/Akt pathway

AIM:To investigate the relationship of microRNA-7 (miRNA-7) over-expression and epidermal growth factor receptor (EGFR)/phosphatidylinositol kinase-3 (PI3K)/protein kinase B (PKB, also called Akt) pathway in human nasopharyngeal carcinoma 5-8F cells. METHODS:The 5-8F cells were transfected with miRNA-7 mimics (carrying by Lipofectamine 2000). The expression of miRNA-7 was detected by real-time PCR. The cell proliferation was analyzed by CCK-8 assay. The cell colony-forming capability was determined by cell colony formation test. The expression of EGFR/PI3K/Akt at mRNA and protein levels was examined by real-time PCR and Western blotting. RESULTS:The expression level of miRNA-7 was significantly increased in 5-8F cells compared with negative control (NC) group and control group (P<0.01). The proliferation of NPC 5-8F cells was decreased extremely after tansfected with the miRNA-7 mimics (P<0.01), so did the result of the cell colony-formation test. The expression of EGFR/PI3K/Akt at mRNA and protein levels was significantly down-regulated compared with NC group and control group (P<0.01). CONCLUSION: Over-expression of miRNA-7 significantly inhibits the proliferation and colony-forming ability of nasopharyngeal carcinoma 5-8F cells by down-regulation of EGFR/PI3K/Akt pathway.     

反刍家畜饲用微生物添加剂的研究与应用

选用贵州省畜牧兽医科学研究所分离鉴定和引购的无毒芽胞杆菌、酵母菌、乳酸菌、粪链球菌等多株益生菌 ,进行培养发酵、配方、生产工艺、主要技术指标等研究 ,研制成反刍家畜饲用微生物添加剂。用 0 2 %～ 0 4%的反刍家畜微生物添加剂对肉牛、山羊补饲精料进行 2 4h发酵处理后饲喂 ,饲养试验表明 ,试验组牛和羊比对照组可分别提高增重 1 4 5 %和 1 2 3 %。     

山毛豆草粉颗粒料对肉兔的饲用价值评定

为探讨山毛豆(Tephrosia candida)草粉对肉兔的饲用价值,将60只新西兰青年肉兔分为5组,分别添加0％(对照),10％,20％,30％和40％的山毛豆草粉制成全价颗粒饲料,饲喂90 d后测定各组饲料的营养成分及肉兔采食量、日增重、料重比和屠宰性能.结果表明:山毛豆营养生长期粗蛋白含量为17.77％,肉兔对山毛豆中粗蛋白的消化率为78.09％,山毛豆的可消化总养分为56.18％.与对照组相比,添加20％草粉日增重达到20.80 g·d-1(P＜0.01);料重比为4.45∶ 1(P＜0.05);屠宰率为57.78％(P＜0.01);单位kg增重平均最低饲料成本差异显著(P＜0.05).因此,添加20％山毛豆草粉制成全价颗粒饲料可显著提高肉兔的生产性能和养殖效益.     

“一带一路”背景下河南食用菌出口贸易发展研究

十多年来我国食用菌产量快速增长,食用菌总产量占全球75%以上。2004—2018年,河南食用菌产量、出口量连续居全国第一。河南自然资源、生产成本的优势,以及相关政策和科研创新的推动,形成了河南食用菌出口贸易发展的有利因素;但由于规划缺位、研发力量薄弱、工厂化发展滞后,河南食用菌在国际市场上还存在品种单一、结构单一、市场过于集中的问题。为此,建议突出食用菌产业的战略地位,做好顶层设计,充分利用好“一带一路”的机遇和本省的资源优势,加强食用菌出口供应链管理,加大研发推广和产业升级力度,营造一流发展环境,提升食用菌国际市场品质,将河南食用菌供应链延伸到更多的国家和地区。     

不同维生素预混料对科宝肉鸡生长性能、胫骨质量和抗氧化能力的影响

试验研究3种维生素预混料(处理1、2、3)对科宝肉鸡生长性能、胫骨质量及抗氧化能力的影响。1 200只科宝肉鸡随机分为3个处理,每个处理10个重复,每个重复40只鸡,试验期42 d。结果表明:与处理1相比,处理3和2可显著提高肉鸡21 d体重,显著降低1～21 d饲料增重比;处理3还显著提高肉鸡1～21 d体增重、采食量和21 d胫骨灰分和磷比例及42 d胫骨灰分、钙比例(P<0.05);与处理2相比,处理3显著提高肉鸡42 d胫骨磷比例(P<0.05)。与处理2和1相比,处理3显著提高肉鸡21 d血清总超氧化物歧化酶(T-SOD)活力(P<0.05)。结果表明,根据科宝肉鸡的生长性能、胫骨质量和机体抗氧化能力,适宜维生素预混料为处理3。     

LED连续光和不同频率间歇光对黄瓜幼苗生长及光合特性的影响

以"津优1号"黄瓜为试材,采用单因素方差分析方法,研究了LED光源不同发光频率对植物幼苗生长发育和光合的影响,以期获得最适宜黄瓜幼苗生长的频率和占空比组合,实现节约能源和优质高产的目标。试验设置连续光(CK)和发光频率分别是0.1 Hz(T1)、100Hz(T2)、100 000Hz(T3)的间歇光共4个处理作为黄瓜生长光源,每个处理3次重复。结果表明:频率越低,黄瓜幼苗的株高和茎叶鲜质量均显著大于对照;频率越高,黄瓜幼苗的茎粗、叶面积、叶绿素含量、暗适应下PSII的最大量子产量均显著大于对照。不同频率的间歇光在一定程度上对黄瓜幼苗的生长和光合作用起促进作用。较低频率的间歇光可能有助于增加黄瓜幼苗干物质的积累量、植株生长率和增强植株的健壮程度;较高频率的间歇光可能有助于黄瓜幼苗的植株形态向有利于光合作用的方向发展。     

梨Ty1-copia类反转录转座子GAG开放阅读框预测和光响应下的表达分析

基于生物信息学方法,对‘砀山酥梨’基因组中Ty1-copia类型的GAG开放阅读框进行预测,共获得315条GAG序列。通过系统聚类,梨基因组中GAG序列可分为7个支系,第一和第三支系成员较多且保守性高。序列比对结果显示,不同支系GAG序列具有较高的异质性。在‘美人酥’梨的套袋和摘袋后的果皮中发现了34个gag成员,其中有6个（Ppgag16、Ppgag19、Ppgag23、Ppgag27、Ppgag29和Ppgag30）为高丰度表达成员。遮光条件下,‘美人酥’果皮中有部分gag成员发生表达,说明copia类反转座子不仅仅受光照影响发生表达。在摘袋后,‘美人酥’果皮中有2个成员（Ppgag5和Ppgag23）发生明显上调表达,说明梨中部分反转录转座子的转录受到光照的调控。     

大棚不同地膜栽培对蔬菜生长及产量的影响

通过在大棚内进行不同地膜,研究了大棚内麻地膜覆盖对辣椒、黄瓜生长及产量的影响。试验结果表明,大棚内麻地膜覆盖栽培促进蔬菜生长,提高辣椒、黄瓜产量。麻地膜覆盖栽培辣椒、黄瓜比聚乙烯农用黑膜增产5．2％、14．5％,比CK（不覆盖）增产24．7％、23．0％,与对照相比均达到显著差异。     

Osmolarity,cell volume and proliferation in nasopharyngeal carcinoma cells

AIM: To investigate the relationship between osmolarity, cell volume and cell proliferation in nasopharyngeal carcinoma cells. METHODS: MTT method was applied to detect the proliferation ability of the poorly-differentiated nasopharyngeal carcinoma cell (CNE-2Z) under various osmolarity conditions. The flow cytometry was used to analyse cell cycle distribution. Cell volume was obtained by the image analysis of living cells and cell viability was determined by the trypan blue assay. RESULTS: Cultivation of cells under the hypertonic conditions of 370 and 440 mOsmol/L increased cell volume by 8.7% and 27.8% and facilitated cell proliferation by 22.2% and 33.9%, respectively. However, hypotonic incubation of cells with osmolarity of 160 and 230 mOsmol/L decreased cell volume by 12.8% and 4.1% and inhibited cell proliferation by 34.0% and 15.6%, respectively. Cell volume was positively correlated with cell proliferation rate. Long-term cultivation of cells under anisotonic conditions did not significantly alter cell cycle distribution, but hypotonic cultivation decreased cell viability. CONCLUSION: Proliferation of nasopharyngeal carcinoma cells was closely correlated with the osmolarity of culture medium and cell volume. Hypotonic cultivation may inhibit cell proliferation by decreasing cell volume to facilitate cell death mechanisms.     

取食不同猎物对黄带犀猎蝽生长发育和繁殖的影响

黄带犀猎蝽Sycanus croceovittatus可捕食多种害虫, 是农林业害虫生物防治的重要天敌昆虫?为评价黄粉虫Tenebrio molitor?果蝇Drosophila sp.?斜纹夜蛾Spodoptera litura和棉蚜Aphis gossypii 4种猎物作为规模化饲养黄带犀猎蝽的食物来源的效果, 在室内温度(25±1)℃, 相对湿度(60±5)%, 光周期L∥D=14 h∥10 h条件下, 测定了分别取食上述4种猎物的黄带犀猎蝽生长发育和繁殖的相关生物学参数?结果表明, 黄带犀猎蝽以这4种猎物为食均可完成1个世代, 且可以继续繁殖, 但猎物种类对其相关生物学参数有显著影响?取食斜纹夜蛾的黄带犀猎蝽成虫获得率(69.9±7.6)%, 若虫总历期(70.3±0.6) d, 产卵前期(21.5±0.5) d, 卵期(20.0±0.1) d, 单雌产卵量(213.4±22.7)粒, 卵孵化率(76.5±3.6)%?其若虫死亡率较低, 成虫获得率较高, 若虫发育历期最短, 各发育阶段的黄带犀猎蝽体重最重?与黄粉虫组相比, 其成虫产卵前期显著缩短, 卵孵化率显著提高, 但成虫寿命较短?说明斜纹夜蛾幼虫可作为室内扩繁黄带犀猎蝽的猎物?