茶树CsSPMS基因全长cDNA克隆和时空表达分析

为探究精胺合成酶（spermine synthase,SPMS）与茶树（Camellia sinensis）耐寒性的关系,以茶树品种‘迎霜’为试验材料,利用简并引物PCR扩增技术结合5′/3′RACE方法,获得与低温胁迫相关的CsSPMS片段cDNA全长序列（GenBank登录号为KJ580429）。该序列全长1 374 bp,包含1 113 bp的完整开放阅读框（ORF）,编码371个氨基酸,预测分子量41.28 kD;构建亚细胞定位载体pJIT166-GFP/SPMS,亚细胞定位结果显示CsSPMS定位在细胞核上。荧光定量PCR分析表明CsSPMS在根、茎、叶、芽、花和果中都有表达,在根、茎中表达量较高;低温胁迫处理下不同组织CsSPMS的表达量,在叶片中2 h达到峰值,而在根中12 h达到高峰;在低温条件下4个茶树品种的耐寒性与CsSPMS表达量密切相关。     

Mechanism of Chinese processing in patients with Broca aphasia

AIM: To explore the mechanism of Chinese processing in patients with Broca aphasia so as to offering accordance for speech rehabilitation. METHODS: The test of orthographic, semantic information and phonological were conducted. The scores in each group with Chi-square test in patients were compared to those in 10 normal people. RESULTS: No significant difference between two groups in the vocabulary identification and in the vocabulary identification from homophony (P>0.05) was observed (P>0.05). There was significant difference in the identification picture from vocabularies, the identification vocabulary from pictures and the vocabulary identification from sound (P>0.05). CONCLUSION: The patient with Broca aphasia is independent in the course of the orthographic input, and the ability to retrieve semantic information from orthographic activation word may not affected by phonological lexicon.     

重庆开县3个锦橙品种留树贮藏试验初报

对重庆市开县3个锦橙品种留树贮藏效果进行探索,结果表明,‘开陈72-1’、‘开盛76-8’和‘北碚447’3个锦橙品种留树贮藏至2月下旬,能维持较高的坐果率、商品果率、外观色泽和可溶性固形物含量;‘开陈72-1’最适宜做开县锦橙留树贮藏品种。     

无子沙糖橘(十月橘)的选育

采用芽变选种的方法,在四会市石狗镇选出了无子柑橘新品种—无子沙糖橘(十月橘)。果略小,单果重平均40g左右,单果平均种子数0.5粒以内,达到柑橘无子的标准。果实扁平,皮橘红色,薄而脆;果肉橙黄色,味清甜,可溶性固形物13%以上,肉质爽脆化渣,品质上等。早结丰产性好,发梢能力强,3月上中旬开花,12月上旬果实开始成熟。     

Effects of baicalin on CA46 cell xenografts in nude mice

AIM: To study the effects of baicalin on CA46 cell xenografts in nude mice. METHODS: The nude mice with CA46 cell xenografts were treated with drugs via intraperitoneal injection daily, and were divided into 5 groups: negative control group, 15 mg/kg baicalin group, 30 mg/kg baicalin group, 60 mg/kg baicalin group and 4 mg/kg etoposide (VP-16) positive control group. After 12-day treatment, the weight of CA46 cell xenografts stripped from some nude mice in the 5 groups was used to evaluate the effect of baicalin on xenograft growth in the nude mice. The apoptosis, necrosis and pathological changes of the xenograft cells were examined under light microscope and transmission electronic microscope respectively. The expression levels of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway-related proteins extracted from xenografts were determined by Western blotting. The other nude mice with CA46 cell xenografts in the 5 groups continued to be treated with the drugs until death in order to evaluate the effect of balcalin on survival time of the nude mice with CA46 cell xenografts. RESULTS: Baicalin remarkably inhibited the growth of CA46 cell xenografts, induced apoptosis and necrosis of xenograft cells, and reduced the protein expression of phospho-Akt (p-Akt), nuclear factor-kappa B (NF-κB), mammalian target of rapamycin (mTOR) and phospho-mTOR (p-mTOR) in the xenografts after 12-day treatment. Furthermore, baicalin prolonged the survival time of the nude mice with CA46 cell xenografts in a dose-dependent manner. CONCLUSION: Baicalin inhibits the growth and induces apoptosis of CA46 cell xenografts in the nude mice, and prolongs the survival time of the nude mice with CA46 cell xenografts through the mechanism of down-regulating PI3K/Akt/NF-κB and PI3K/Akt/ mTOR signaling pathways.     

番茄单性结实研究进展

对番茄单性结实资源、评价鉴定方法、子房内源激素水平、分子机制及新品种选育等的研究现状进行了综述,尤其对近几年分子水平的最新研究进行了评述,对今后这一领域的研究做了展望。     

白莲腐败病病原菌的分离鉴定

莲腐败病是白莲种植区发生最普遍、为害最严重的病害之一。本试验对发病白莲上的病原茵进行分离鉴定。该病原分离物在PSA培养基上气生茵丝由白色渐变成深紫色,并产生大小两种孢子。大型分生孢子镰刀形,无色,稍呈弯曲,两端尖细,大小为（39．1-55．2）μm×（2．8～4．6）p,m,多为3个隔膜,少部分为1-2隔或4~5隔;小型分生孢子椭圆形。无色,无隔膜,大小为（5．0～9．2）μm×（2．0～3．5）μm。致病性测定表明分离物能100％引起白莲出现典型的白莲腐败病的症状。ITSl／ITS4的PCR扩增产物与GenBank中Fusariumoxysporum￡sp．nelumbicola的序列同源性为99％。研究结果表明。分离到的病原物为尖孢镰刀茵莲专化型Foxysporum f.sp．ndumbicola。     

新疆野生樱桃李SSR体系的建立及应用

为建立适宜新疆野生樱桃李的SSR分子标记技术体系,通过对PCR反应程序、反应体系(DNA模板量、引物浓度、Mg2+浓度、dNTP浓度、Taq酶用量)以及退火温度进行了探索,建立了适宜野生樱桃李的SSR-PCR反应体系。结果表明:在25μL反应体系中,Mg2+1.0 mmol/L、引物0.5μmol/L、dNTPs 0.20 mmol/L、TaqDNA聚合酶1.0 U、模板DNA 30 ng、退火温度为60℃。SSR扩增程序:94℃预变性5 min,35个循环(94℃30 s,60℃1 min,72℃1 min),72℃延伸10 min,可筛选出稳定性好、多态性高的SSR引物。     

武汉市园林绿地群落结构研究

在武汉市城区范围内,采用典型抽样的方法,对武汉市园林绿地植物种类组成、群落结构现状和主要配置模式进行了详细的调查和分析.结果表明:武汉市园林绿地常用绿化植物比较丰富,生长状况良好,植被群落以乔灌草复合结构为主,比例达56.49％,主要的配植模式为常绿乔+落叶乔-常绿灌+落叶灌-草本层.结合武汉市常用的绿化植物建立了3个理想化配置模式,以期为武汉城市园林绿地的规划与管理提供参考依据.     

MdMYB73的分子克隆及其在苹果愈伤组织和拟南芥幼苗中的盐抗性功能鉴定

从‘嘎拉’苹果中克隆了一个MYB转录因子基因(序列号:MDP0000894463)。该基因包含长为729 bp完整的开放阅读框,编码243个氨基酸,预测其蛋白质分子量为26.34 kD,等电点为9.29。系统进化树分析表明,这一MYB转录因子与拟南芥AtMYB73同源序列相似性最高,因此将其命名为MdMYB73。功能域分析表明,MdMYB73蛋白含有保守的R2R3-typeMYB绑定域。荧光定量PCR分析表明,MdMYB73在苹果的各个组织均有表达,在叶片和花中表达相对较高;MdMYB73的表达明显受盐胁迫的诱导。将异位表达MdMYB73的拟南芥幼苗进行抗盐鉴定,结果表明MdMYB73负调控拟南芥盐胁迫抗性;同时,AtSOS1,AtSOS3和AtNHX1抗盐相关基因的表达水平显著降低,表明MdMYB73可能负调控SOS反应,影响拟南芥抵抗高盐胁迫过程。将MdMYB73基因遗传转化苹果愈伤组织,抗盐表型分析表明,MdMYB73过量表达也明显降低了转基因愈伤组织对盐胁迫的抗性。