全文获取类型
收费全文 | 87293篇 |
免费 | 5188篇 |
国内免费 | 42篇 |
专业分类
林业 | 2978篇 |
农学 | 2711篇 |
基础科学 | 526篇 |
9920篇 | |
综合类 | 16188篇 |
农作物 | 3655篇 |
水产渔业 | 4303篇 |
畜牧兽医 | 45546篇 |
园艺 | 1082篇 |
植物保护 | 5614篇 |
出版年
2019年 | 807篇 |
2018年 | 1193篇 |
2017年 | 1376篇 |
2016年 | 1291篇 |
2015年 | 1145篇 |
2014年 | 1361篇 |
2013年 | 3453篇 |
2012年 | 2557篇 |
2011年 | 3040篇 |
2010年 | 1979篇 |
2009年 | 1948篇 |
2008年 | 2934篇 |
2007年 | 2836篇 |
2006年 | 2688篇 |
2005年 | 2535篇 |
2004年 | 2384篇 |
2003年 | 2412篇 |
2002年 | 2275篇 |
2001年 | 2904篇 |
2000年 | 2782篇 |
1999年 | 2268篇 |
1998年 | 975篇 |
1997年 | 893篇 |
1996年 | 790篇 |
1995年 | 957篇 |
1994年 | 863篇 |
1993年 | 857篇 |
1992年 | 1812篇 |
1991年 | 1811篇 |
1990年 | 1751篇 |
1989年 | 1816篇 |
1988年 | 1570篇 |
1987年 | 1680篇 |
1986年 | 1739篇 |
1985年 | 1665篇 |
1984年 | 1312篇 |
1983年 | 1167篇 |
1982年 | 842篇 |
1981年 | 723篇 |
1979年 | 1107篇 |
1978年 | 900篇 |
1977年 | 781篇 |
1976年 | 734篇 |
1975年 | 810篇 |
1974年 | 999篇 |
1973年 | 1041篇 |
1972年 | 994篇 |
1971年 | 946篇 |
1970年 | 887篇 |
1969年 | 811篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
Summary Effects of weekly (W) and fortnightly (F) irrigation schedules on established stands of lucerne (Medicago sativa L.) grown on gypsum treated (G) and untreated (C) heavy clay soil were investigated. Two irrigations were applied under the fortnightly regime and four under the weekly schedule during a single cutting cycle. Growth and light interception were measured during both the vegetative and mature phases of growth.Leaf expansion, light interception and dry matter production were greater under treatments W
G and W
C, with yield increasing from 3.4 t ha–1 under the fortnightly schedule to 5.0 t ha-1 with weekly irrigation. Gypsum treatment was effective under the more frequent irrigation schedule. Specific leaf area and the proportion of stem were both increased by treatments W
G and W
C. The responses to irrigation were therefore characteristic of those elicited by a more favourable plant moisture status. Growth was analysed in terms of light interception, the efficiency of utilisation of intercepted light, the proportion of the daily dry matter gain retained by the leaves and leaf expansion. The analysis demonstrated that impaired leaf expansion contributed to a decline of approximately 15% in yield, and that impaired efficiencies of utilisation of intercepted light contributed to losses of approximately 30% under the less frequent irrigation schedule.A comparison of growth rates and efficiencies of energy conversion with published data showed that satisfactory rates of growth and levels of productivity were achieved on the heavy soils of the local region using gypsum treatment and the more frequent irrigation schedule. 相似文献
992.
The esterification and hydrolytic activities of free and immobilized Candida rugosa lipase isoform 1 (LIP1) were investigated. Esterification activity was determined by reacting caprylic acid with glycerol in the presence of molecular sieves (30%, w/w), and the volume of 1.0 M NaOH consumed by the reaction products upon titration was used to calculate esterification activity. Caprylic acid was also reacted with cottonseed oil, and the amount of caprylic acid incorporated after 12 h of reaction was determined. Results indicated that LIP1 had little esterification activity, which was not significantly improved upon immobilization. Hydrolytic activity was determined by incubating tricaprylin emulsion (15%, w/w) with the respective lipases for 60 min, and the reaction products were titrated against 0.5 M NaOH. LIP1 showed hydrolytic activity comparable to Lipozyme RM IM. The hydrolytic activity improved significantly upon immobilization. Immobilization on Celite 545 produced the highest increase in hydrolytic activity. 相似文献
993.
Mahendran T Williams PA Phillips GO Al-Assaf S Baldwin TC 《Journal of agricultural and food chemistry》2008,56(19):9269-9276
The structural characteristics of the gum exudate of Acacia senegal (gum arabic) have been investigated by monitoring the composition and physicochemical properties before and after treatment with proteolytic enzyme and various alkaline systems. Molecular mass ( M w) and radius of gyration ( R g) measurements were performed using gel permeation chromatography (GPC) coupled to refractive index, UV absorbance, and multiangle light scattering detectors and indicated that the macromolecules present have a compact structure. It was found that treatment with proteolytic enzyme caused the arabinogalactan-protein component (AGP) with average molecular mass approximately 2 x 10 (6) Da to degrade, yielding material of molecular mass approximately 4 x 10 (5) Da, whereas the bulk of the material corresponding to the protein-deficient arabinogalactan component (AG) with molecular mass 4 x 10 (5) remained unaffected. Barium hydroxide was found to hydrolyze the polysaccharide component (AG) itself in addition to the proteinaceous component as demonstrated in control experiments using dextran. However, sodium borohydride/sodium hydroxide treatments were unable to hydrolyze dextran and were assumed to hydrolyze only the proteinaceous component of gum arabic. The AGP component was completely degraded, yielding material of molecular mass approximately 4.5 x 10 (4) Da. It has been concluded, therefore, that the enzyme did not fully hydrolyze all of the protein present and that the AGP component of gum arabic consists of carbohydrate blocks of approximately 4.5 x 10 (4) Da linked to a polypeptide chain consistent with the wattle blossom structure. Because the AGP was degraded to differing extents using a mild and more severe sodium borohydride/sodium hydroxide treatment, it was concluded that the polysaccharide moieties were linked through both O-serine and O-hydroxyproline residues. The gum arabic sample was deglycosylated by treatment with anhydrous hydrogen fluoride and revealed the presence of two putative core proteins of approximately 3 x 10 (4) and approximately 5 x 10 (3) Da, respectively, which correspond to proteins of approximately 250 and 45 amino acids in length. A new model for the structure of the AGP component has been proposed. 相似文献
994.
Rizzello CG Cassone A Di Cagno R Gobbetti M 《Journal of agricultural and food chemistry》2008,56(16):6936-6943
This article aimed at investigating the synthesis of angiotensin I-converting enzyme (ACE)-inhibitory peptides and gamma-aminobutyric acid (GABA) during sourdough fermentation of white wheat, wholemeal wheat, and rye flours. Sourdough lactic acid bacteria, selected previously for proteinase and peptidase activities toward wheat proteins or for the capacity of synthesizing GABA, were used. The highest ACE-inhibitory activity was found by fermenting flour under semiliquid conditions (dough yield 330) and, especially, by using wholemeal wheat flour. Fourteen peptides, not previously reported as ACE-inhibitory, were identified from the water/salt-soluble extract of wholemeal wheat sourdough (IC 50 0.19-0.54 mg/mL). The major part of the identified peptides contained the well-known antihypertensive epitope VAP. The synthesis of GABA increased when the dough yield was decreased to 160. The highest synthesis of GABA (258.71 mg/kg) was found in wholemeal wheat sourdough. 相似文献
995.
Nicola C. Okes Philip A.R. Hockey Lorien Pichegru Carl D. van der Lingen Robert J.M. Crawford David Grmillet 《Biological conservation》2009,142(10):2361-2368
In the southern Benguela upwelling ecosystem off the west coast of South Africa, seabird populations are decreasing dramatically because of reduced availability of pelagic fish. We tested the hypothesis that the west coast fishing industry is competing for the remaining stocks of anchovy and sardine with the largest colony of Vulnerable Cape Gannets (Morus capensis) along the Atlantic coast. Using GPS-tracking of the birds, echo-sounding of pelagic fish, and vessel log books, we located overlap areas between bird foraging ranges, pelagic fish distribution, and fishing activities. We then compared fish catches by gannets and vessels within their joint foraging zones. In October 2007, purse-seine fishing grounds and gannet foraging areas overlapped by only 13%. However, for a 1-month period, the amount of fish removed from this area by purse-seine boats amounted to 41% of the food requirements of the 72,000 gannets breeding on Malgas Island (25% of the world population). The fishery’s catch in this area is significant in terms of its potential impact on gannets, but comprises only 3.6% of total fishery catch. Based on this finding, the rapidly decreasing size of the gannet colony and the stated objectives of South Africa’s Marine Living Resources Act of 1998, the case for considering and experimenting with at-sea ‘no-take’ areas for the purse-seine fishery is strong. Efforts to establish whether ‘no-take’ fishing zones increase food availability for top predators is an important next step in conservation of the southern Benguela Ecosystem. 相似文献
996.
Residue quality has been shown to influence soil water-stable aggregation (WSA) during crop residue decomposition, but there is still little information about its interactive effect with soil mineral N availability. The aim of this study was to determine the effect of soil mineral N on WSA during the decomposition of two high-C/N crop residues (wheat straw with C/N = 125.6 and miscanthus straw with C/N = 311.3). The two crop residues were combined with three mineral N addition rates (0, 60, and 120 mg N kg−1 dry soil). Respiration, soil mineral N content, and WSA (expressed as mean-weight diameter, MWD) were measured on several dates during a 56-d incubation. The effect of decomposing crop residues on WSA followed two phases. (i) Between 0 and 7 d, the increase in WSA was related to intrinsic residue quality with higher decomposability of the wheat straw resulting in higher WSA. (ii) Thereafter, and until the end of the experiment, mineral N addition rates had a predominant but negative influence on WSA. In this second phase, the average MWD of residue-treated soils was 0.92, 0.55, and 0.44 mm for the 0, 60 and 120 mg N kg−1 dry soil addition rates, respectively. Mineral N addition which did result in higher crop residue decomposition did not lead to higher WSA. WSA during crop residue decomposition is therefore not simply positively related to the induced microbial activity, and changes in microbial community composition with differential effects on WSA must be involved. The impact of high-C/N crop residues inputs on WSA, initially assumed to be low, could actually be strong and long-lasting in situations with low soil mineral N content. 相似文献
997.
Introduced pests threaten many species and their control is generally beneficial for conservation, particularly on islands where complete eradication is possible. Unfortunately on ‘nearshore’ islands neighbouring source populations exist and unaided reinvasion is likely. Pest control programmes at these sites thus require a metapopulation context to adequately manage movements between source and sink populations. We investigated the ecology of introduced ship rats (Rattus rattus) on a nearshore island, and gene flow with adjacent mainland populations, in order to understand the metapopulation dynamics and relative levels of pest control required within the landscape. We sampled the entire population by trapping (n = 30), achieving eradication, and found a low rat density (3.2 ha−1) indicative of a sink population. Seed and other plant material constituted the major dietary component of rats. Despite its proximity to mainland source populations, the island population was genetically distinct with reduced allelic diversity caused by a recent reinvasion founder effect. Genetic analyses also detected recent migrants between the populations. In contrast, two mainland populations separated by a similar distance displayed complete genetic mixing. The small water gap therefore provides a sufficient barrier to lower the migration rate to the island and delay reinvasion, which nonetheless eventually happens. In order to maintain nearshore islands pest-free, conservation management will require a metapopulation approach simultaneously focusing on both island and source population pest control. 相似文献
998.
Diana R. Nemergut Cory C. Cleveland Christopher L. Washenberger 《Soil biology & biochemistry》2010,42(12):2153-2160
Little is known about the organisms responsible for decomposition in terrestrial ecosystems, or how variations in their relative abundance may influence soil carbon (C) cycling. Here, we altered organic matter in situ by manipulating both litter and throughfall inputs to tropical rain forest soils, and then used qPCR and error-corrected bar-coded pyrosequencing to investigate how the resulting changes in soil chemical properties affected microbial community structure. The plot-scale manipulations drove significant changes in microbial community composition: Acidobacteria were present in greater relative abundance in litter removal plots than in double-litter plots, while Alphaproteobacteria were found in higher relative abundance in double-litter and throughfall reduction plots than in control or litter removal plots. In addition, the bacterial:archaeal ratio was higher in double-litter than no-litter plots. The relative abundances of Actinobacteria, Alphaproteobacteria and Gammaproteobacteria were positively correlated with microbial biomass C and nitrogen (N), and soil N and C pools, while acidobacterial relative abundance was negatively correlated with these same factors. Bacterial:archaeal ratios were positively correlated with soil moisture, total soil C and N, extractable ammonium pools, and soil C:N ratios. Additionally, bacterial:archaeal ratios were positively related to the relative abundance of Actinobacteria, Gammaproteobacteria, and Actinobacteria, and negatively correlated to the relative abundance of Nitrospira and Acidobacteria. Together, our results support the copiotrophic/oligotrophic model of soil heterotrophic microbes suggested by Fierer et al. (2007). 相似文献
999.
1000.
Johnson TC 《Science (New York, N.Y.)》1976,192(4242):887-890
A map of the preservation pattern of siliceous microfossils was constructed from an examination of 125 piston and gravity cores of Quaternary sediments from throughout the eastern tropical Pacific. Preservation is enhanced where high surface water productivity supplies a high input rate of siliceous tests to the sea floor, except in a large area north of 5 degrees N, east of the East Pacific Rise. Here a high input rate of terrigenous silicates may adversely affect preservation of biogenic opal in the sea-floor sediments. 相似文献