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排序方式: 共有175条查询结果,搜索用时 656 毫秒
71.
Thomas J. DELIBERTO Seth R. SWAFFORD Dale L. NOLTE Kerri PEDERSEN Mark W. LUTMAN Brandon B. SCHMIT John A. BAROCH Dennis J. KOHLER Alan FRANKLIN 《Integrative zoology》2009,4(4):426-439
As part of the USA's National Strategy for Pandemic Influenza, an Interagency Strategic Plan for the Early Detection of Highly Pathogenic H5N1 Avian Influenza in Wild Migratory Birds was developed and implemented. From 1 April 2006 through 31 March 2009, 261 946 samples from wild birds and 101 457 wild bird fecal samples were collected in the USA; no highly pathogenic avian influenza was detected. The United States Department of Agriculture, and state and tribal cooperators accounted for 213 115 (81%) of the wild bird samples collected; 31, 27, 21 and 21% of the samples were collected from the Atlantic, Pacific, Central and Mississippi flyways, respectively. More than 250 species of wild birds in all 50 states were sampled. The majority of wild birds (86%) were dabbling ducks, geese, swans and shorebirds. The apparent prevalence of low pathogenic avian influenza viruses during biological years 2007 and 2008 was 9.7 and 11.0%, respectively. The apparent prevalence of H5 and H7 subtypes across all species sampled were 0.5 and 0.06%, respectively. The pooled fecal samples (n= 101 539) positive for low pathogenic avian influenza were 4.0, 6.7 and 4.7% for biological years 2006, 2007 and 2008, respectively. The highly pathogenic early detection system for wild birds developed and implemented in the USA represents the largest coordinated wildlife disease surveillance system ever conducted. This effort provided evidence that wild birds in the USA were free of highly pathogenic avian influenza virus (given the expected minimum prevalence of 0.001%) at the 99.9% confidence level during the surveillance period. 相似文献
72.
Donnelly BP Nixon AJ Haupt JL Dahlgren LA 《American journal of veterinary research》2006,67(7):1218-1225
OBJECTIVE: To characterize the nucleotide sequence of equine platelet-derived growth factor (PDGF)-A and -B and analyze temporal expression of these genes in equine tendon after induced tendinitis injury. Animals-18 mature horses. PROCEDURES: Genes for equine PDGF-A and -B were reverse transcribed and sequenced from synovial tissue mRNA obtained from a 3-year-old horse. Collagenase-induced lesions were created in the tensile region of the superficial digital flexor tendon in 14 horses; 3 horses served as uninjured control animals. Tendons were harvested and total RNA was isolated from experimental horses 1, 2, 4, 8, and 24 weeks after collagenase injection. Temporal gene expression for PDGF-A and -B was determined by use of quantitative PCR analysis. RESULTS: Equine PDGF-A shared 83.8% sequence and 87.5% peptide homology with human PDGF-A, with a discrepancy of 70 bp from the human sequence. Equine PDGF-B was similar in length to the human gene, sharing 90.3% and 91.7% nucleotide and peptide identity, respectively. Expression of PDGF-A mRNA in collagenase-induced tendinitis lesions was unchanged, compared with expression for normal control tendon, and remained steady throughout the 24-week study. Expression of PDGF-B mRNA decreased over time, and the expression at 24 weeks was significantly reduced, compared with expression in normal and acutely injured tendon. CONCLUSIONS AND CLINICAL RELEVANCE: Injured tendon mounts a minimal constitutive PDGF-A or -B mRNA response. Serial exogenous treatment with either PDGF isoform within the first 2 to 4 weeks after tendon injury may bolster the meager PDGF paracrine-autocrine intrinsic response to injury. 相似文献
73.
Cunningham CP Brown JM Jacobson GA Brandon MR Martinod SR 《American journal of veterinary research》2006,67(9):1564-1569
OBJECTIVE: To evaluate use of covered-rod (CR) silicone implants containing ivermectin for long-term prevention of infection with Dirofilaria immitisin dogs. ANIMALS: 145 adult male and female dogs. PROCEDURES: Dogs received implants of different sizes, and ivermectin concentrations and serum ivermectin concentrations were monitored for 16, 57, and 56 weeks, respectively, in 3 preclinical dose selection studies. Ability of implants to prevent infection with D immitis was evaluated in 2 further studies; dogs were challenged with 50 infective third-stage larvae 52 weeks after implant administration and necropsied 145 days after challenge, and the total number of adult heartworms was counted. A field study was then undertaken in which client-owned dogs received an implant and plasma samples were collected at intervals until week 52 for ivermectin analysis and heartworm antigen determination. RESULTS: Use of the implants resulted in maintenance of an ivermectin concentration > or = 0.2 ng/mL for 12 months. In challenge studies, no treated dogs had adult heartworms, in contrast to untreated dogs, which all had adult heartworms at necropsy. In the field study, dogs treated with an implant had negative results of heartworm antigen testing for 12 months. CONCLUSIONS AND CLINICAL RELEVANCE: The CR silicone implant containing 7.3 mg of ivermectin was 100% effective in preventing experimental infection with D immitislarvae and resulted in negative results for heartworm antigen in a field trial. This product has the potential to alleviate poor owner compliance with monthly prevention regimens. 相似文献
74.
State-and-Transition Models for Heterogeneous Landscapes: A Strategy for Development and Application
Brandon T. Bestelmeyer Arlene J. Tugel George L. Peacock Daniel G. Robinett Pat L. Shaver Joel R. Brown Jeffrey E. Herrick Homer Sanchez Kris M. Havstad 《Strength and Conditioning Journal》2009,62(1):1-15
Interpretation of assessment and monitoring data requires information about how reference conditions and ecological resilience vary in space and time. Reference conditions used as benchmarks are often specified via potential-based land classifications (e.g., ecological sites) that describe the plant communities potentially observed in an area based on soil and climate. State-and-transition models (STMs) coupled to ecological sites specify indicators of ecological resilience and thresholds. Although general concepts surrounding STMs and ecological sites have received increasing attention, strategies to apply and quantify these concepts have not. In this paper, we outline concepts and a practical approach to potential-based land classification and STM development. Quantification emphasizes inventory techniques readily available to natural resource professionals that reveal processes interacting across spatial scales. We recommend a sequence of eight steps for the co-development of ecological sites and STMs, including 1) creation of initial concepts based on literature and workshops; 2) extensive, low-intensity traverses to refine initial concepts and to plan inventory; 3) development of a spatial hierarchy for sampling based on climate, geomorphology, and soils; 4) stratified medium-intensity inventory of plant communities and soils across a broad extent and with large sample sizes; 5) storage of plant and soil data in a single database; 6) model-building and analysis of inventory data to test initial concepts; 7) support and/or refinement of concepts; and 8) high-intensity characterization and monitoring of states. We offer a simple example of how data assembled via our sequence are used to refine ecological site classes and STMs. The linkage of inventory to expert knowledge and site-based mechanistic experiments and monitoring provides a powerful means for specifying management hypotheses and, ultimately, promoting resilience in grassland, shrubland, savanna, and forest ecosystems. 相似文献
75.
Mueller SN Hosiawa-Meagher KA Konieczny BT Sullivan BM Bachmann MF Locksley RM Ahmed R Matloubian M 《Science (New York, N.Y.)》2007,317(5838):670-674
The chemokines CCL21 and CXCL13 are immune factors that dictate homing and motility of lymphocytes and dendritic cells in lymphoid tissues. However, the means by which these chemokines are regulated and how they influence cell trafficking during immune responses remain unclear. We show that CCL21 and CXCL13 are transiently down-regulated within lymphoid tissues during immune responses by a mechanism controlled by the cytokine interferon-gamma. This modulation was found to alter the localization of lymphocytes and dendritic cells within responding lymphoid tissues. As a consequence, priming of T cell responses to a second distinct pathogen after chemokine modulation became impaired. We propose that this transient chemokine modulation may help orchestrate local cellularity, thus minimizing competition for space and resources in activated lymphoid tissues. 相似文献
76.
A genome-wide association study of type 2 diabetes in Finns detects multiple susceptibility variants
Scott LJ Mohlke KL Bonnycastle LL Willer CJ Li Y Duren WL Erdos MR Stringham HM Chines PS Jackson AU Prokunina-Olsson L Ding CJ Swift AJ Narisu N Hu T Pruim R Xiao R Li XY Conneely KN Riebow NL Sprau AG Tong M White PP Hetrick KN Barnhart MW Bark CW Goldstein JL Watkins L Xiang F Saramies J Buchanan TA Watanabe RM Valle TT Kinnunen L Abecasis GR Pugh EW Doheny KF Bergman RN Tuomilehto J Collins FS Boehnke M 《Science (New York, N.Y.)》2007,316(5829):1341-1345
Identifying the genetic variants that increase the risk of type 2 diabetes (T2D) in humans has been a formidable challenge. Adopting a genome-wide association strategy, we genotyped 1161 Finnish T2D cases and 1174 Finnish normal glucose-tolerant (NGT) controls with >315,000 single-nucleotide polymorphisms (SNPs) and imputed genotypes for an additional >2 million autosomal SNPs. We carried out association analysis with these SNPs to identify genetic variants that predispose to T2D, compared our T2D association results with the results of two similar studies, and genotyped 80 SNPs in an additional 1215 Finnish T2D cases and 1258 Finnish NGT controls. We identify T2D-associated variants in an intergenic region of chromosome 11p12, contribute to the identification of T2D-associated variants near the genes IGF2BP2 and CDKAL1 and the region of CDKN2A and CDKN2B, and confirm that variants near TCF7L2, SLC30A8, HHEX, FTO, PPARG, and KCNJ11 are associated with T2D risk. This brings the number of T2D loci now confidently identified to at least 10. 相似文献
77.
Peridotite xenoliths from the Cascade arc in the United States and in the Japan arc have neodymium and osmium isotopic compositions that are consistent with addition of 5 to 15 percent of subducted material to the present-day depleted mantle. These observations suggest that osmium can be partitioned into oxidized and chlorine-rich slab-derived fluids or melts. These results place new constraints on the behavior of osmium (and possibly other platinum group elements) during subduction of oceanic crust by showing that osmium can be transported into the mantle wedge. 相似文献
78.
Brandon C. Moore Wan-Nor Fitri Lauren Augustine 《Anatomia, histologia, embryologia》2020,49(3):390-401
As wild population threats for the endangered false gharial (Tomistoma schlegelii) persist, conservation breeding programs, including developing semen collection techniques for subsequent artificial insemination, are becoming important species conservation measures. Developing reproductive biology understanding of a species is important to developing best practices and hopefully maximizing reproductive successes. However, information on crocodylians functional copulatory anatomy in general is lacking. To that end, zoological facilities and conservation centres have the exceptional opportunity to contribute new understandings that may not otherwise be attainable regarding crocodylian reproductive anatomy, particularly during routine physical examinations or post-mortem necropsies. Therefore, to better understand T. schlegelii reproductive biology, to contribute knowledge in support of zoo breeding conservation efforts and to contribute to what is known overall about crocodylian reproduction, we investigated phallic anatomy of adult male Tomistoma from two zoological populations, the St. Louis Zoo, USA and Sungai Dusun Wildlife Reserve, Peninsular Malaysia. Here, we present the gross anatomical features and histological analysis of underlying tissue-level details in pursuit of a better understanding of copulatory function and associated gamete transfer mechanisms. While much of the overall Tomistoma phallic morphology and inferred function corresponds to that of other crocodylian species and speaks to conserved aspects of functional anatomy across taxa, species-specific aspects of glans and glans tip morphology are also identified. These novelties are discussed in a general function and overall broader evolutionary contexts. 相似文献
79.
Brumm Kyle J. Hanks R. Daniel Baldwin Robert F. Peoples Brandon K. 《Landscape Ecology》2022,37(10):2589-2605
Landscape Ecology - Cross-scale analyses are central to understanding patterns and processes in hierarchically structured ecological systems. Systematic conservation planning has progressed in... 相似文献
80.