Chemical Composition of Rainwater Near Two Historical Monuments The Thann Collegiate (Alsace,France) and the Tours Cathedral (Indre et Loire,France)

In the present study, included within the framework of a Franco-German Research Program for Conservation of HistoricalMonuments, the chemical composition of rainwater was investigated in Thann (Alsace, France) and in Tours (Indre etLoire, France) between 1992 and 1993. A total of 78 and 24samples, respectively, were collected, near the Thann collegiateand the Tours cathedral on a weekly basis and analysed for Cl^-, NO₃ ^-, SO₄ ^2-, Na⁺, NH₄ ⁺, K⁺, Mg²⁺ and Ca²⁺. Results showpH ranged from 3.60 to 6.58 and from 5.49 to 7.01 for Thann andTours, respectively. In Thann, SO₄ ^2- is the major anion and rainwater acidity is neutralised by compounds ofterrigenic origin which come from the soil of the upper Rhinevalley (Ca²⁺; ‘loess’) and the potash mines of Alsace. In Tours, SO₄ ^2- is also the major anion and the acidity is neutralised partially by atmospheric ammonia and partially by compounds of terrigenic origin and by dust fromthe erosion of the cathedral and the works of restoration, in the form of CaCO₃.     

Using Monte Carlo simulations to estimate relative fire ignition danger in a low-to-medium fire-prone region

A comprehensive assessment of fire ignition danger is nowadays a basic step towards the prioritization of fire management measures. In this study we propose performing a fire selectivity analysis using Monte Carlo simulations to statistically estimate the relative fire ignition danger in a low-to-intermediate fire-prone region such as Canton Ticino, Switzerland. We define fire ignition danger as the likelihood that at a given place a fire will be ignited. For each 25 m × 25 m pixel of the study area, landscape characteristics that may be related to the probability of fire ignition such as vegetation type, elevation, aspect, slope, urban-forest interface were first split into 9-12 categories. The selectivity of each category with respect to fire ignition was then statistically tested by means of Monte Carlo simulations. Finally, we proposed two different approaches for calculating the ignition danger index: cumulating the scores of the Monte Carlo simulations to a final index or producing synthetic scores by performing a principal component analysis of the Monte Carlo results. The validation of the resulting fire danger indices highlights the suitability of both proposed approaches. The PCA-option allows a slightly better discrimination between ignition and non-ignition points and may be of more general application.     

Normal Canine Prostate Gland: Repeatability,Reproducibility, Observer‐Dependent Variability of Ultrasonographic Measurements of the Prostate in Healthy Intact Beagles

Most prostatic diseases in dogs are associated with prostatomegaly, and transabdominal ultrasonography has become the imaging modality of choice for evaluation of the prostate gland in the dog. The aim of the present study was to assess the reproducibility, the repeatability and interobserver variations of sonographic measurements of prostate and to determine which measurement had the lowest variability. Length and height of prostate gland were measured on longitudinal views, width of the prostate gland and height of left and right lobes of the gland on transversal views. The within‐day and between‐day variabilities of the prostatic parameters were determined by performing 1350 (270 length, 270 height, 270 width, 270 height of right lobe and 270 height of left lobe) examinations on ten healthy intact beagle dogs on six different days, in a two‐week period (three days for the five dogs, three different days for the five others). Three observers with different levels of experience in ultrasonography performed the examinations. The lowest within‐day and between‐day standard deviation and coefficient of variation values were observed for the width of the prostate. The width of the gland measured on transverse frozen images seems to be the most reliable measurement for evaluating size of prostate glands in healthy dogs, although the shape, position, outline, and echogenicity of the prostate should also be assessed.     

Heritability of Coffea canephora yield estimated from several mating designs

Eleven mating designs, 6 top-crosses and 5 North Carolina II, were used to estimate narrow sense, broad sense and within-cross heritability of Coffea canephora yield in Côte d'Ivoire. The objective was to predict achievable genetic gains according to different breeding strategies. Narrow sense, broad sense, family and within-cross heritabilities were 0.32, 0.43, 0.83 and 0.22, on average respectively. The percentage of additivity was high (81% on average). A degree of variation was nevertheless observed for estimates over trials. This was found to be mainly due to parent sampling in each trial. By theoretical computation, the minimum number of parents required to obtain optimum precision was found to be around 30. Predicted genetic gains for yield through clonal selection at a selection level of 1% ranged from 7 to 28% over the best hybrid in each trial, suggesting thus clones still interesting for breeders. The consequences for coffee breeding are discussed.     

Chromosomal composition of the genome in the monomorphic diplokaryotic microsporidium Paranosema grylli: analysis by two-dimensional pulsed-field gel electrophoresis

The molecular karyotype of Paranosema grylli Sokolova, Seleznev, Dolgikh et Issi, 1994, a monomorphic diplokaryotic microsporidium, comprises numerous bright and faint bands of nonstoichiometric staining intensity. Restriction analysis of chromosomal DNAs by "karyotype and restriction display" 2-D PFGE has demonstrated that the complexity of molecular karyotype of P. grylli is related to the pronounced length polymorphism of-homologous chromosomes. The background of this phenomenon is discussed in the context of ploidy state, reproductive strategy and population structure in this microsporidium. We propose that the remarkable size variation between homologous chromosomes in P. grylli may be a consequence of ectopic recombination at the chromosome extremities.     

Plant Age and Growing Season Nutritional Content Relationships of Three Artemisia tridentata Subspecies

The effect of plant age on growing season chemical compositions and rumen fermentation characteristics was determined for three subspecies of big sagebrush: basin (Artemisia tridentata [Nutt.] subsp. tridentata), mountain (A. tridentata subsp. vaseyana [Rybd.] Beetle), and Wyoming (A. tridentata subsp. wyomingensis [Beetle and Young]). In vitro dry matter (IVDMD) and organic matter (IVOMD) disappearance, ammonia nitrogen (NH₃N), and volatile fatty acid (VFA) content were determined at the end of two fermentation periods (24 h and 48 h) by combining rumen inocula with age-classified vegetative samples from each sagebrush subspecies. An additional one-way analysis of variance was performed to investigate potential differences among subspecies in IVDMD, IVOMD, total VFA, and NH₃N following a 48-h fermentation period. Crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) components were also compared among sagebrush subspecies. Age class responses were variable across the spectrum of sagebrush subspecies and response variables. Where plant age effects were indicated, the small numeric differences probably have little biological or ecological significance. Mountain sagebrush was lower in IVOMD and total VFA concentrations (P < 0.0001) than basin and Wyoming. NH₃N concentration and CP were higher (P < 0.0001) in basin sagebrush than the other two subspecies, while Wyoming sagebrush was higher in NDF, ADF, and ADL than basin and mountain subspecies (P < 0.0001). NH₃N concentration for all three subspecies was lower than the minimum level (20 mg · 100 mL^?1) required for uninhibited rumen activity. Overall, this research questions the contention that older sagebrush plants offer less nutritional value than younger ones, at least for growing season conditions. The results also provide information that can be utilized in designing supplementation strategies for domestic animals on diets with characteristically high utilization of big sagebrush.     

Short-term watering-distance and symmetry effects on root and shoot growth of bell pepper plantlets

Drip lines were located at distances ranging from 0 to 60 cm from one or both sides of a row of pepper plantlets, and we monitored the effects on their shoot development during 76 days from transplanting to full-size first fruits, on the final root system, and on the areal water and salt distributions in the upper 15-cm soil layer. The experiment was conducted in a greenhouse with a sandy soil, and excess fresh water (1.9 L d⁻¹ per plant) was applied via short daily irrigations. In addition, the effects of watering distance and symmetry on the potential water uptake rate were analyzed with a coupled-source-sink steady flow and uptake model. Initial faster shoot growth with the one-side system and short distances progressively changed to faster growth with the two-side system and longer watering distances, with the optimum at 30-40 cm. These temporal changes are attributed to temporal changes in the root uptake of irrigation water: small plants with small root systems benefit from the larger water supply to a smaller soil volume provided by the one-side system, whereas larger plants with greater water needs could extract more irrigation water when they developed larger, split root systems in the two-side irrigation. Balanced root systems and maximal shoot growth can be obtained by starting the irrigation with a line on each side, near the plants, and moving the lines after a short time.     

Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes

The compact genome of Fugu rubripes has been sequenced to over 95% coverage, and more than 80% of the assembly is in multigene-sized scaffolds. In this 365-megabase vertebrate genome, repetitive DNA accounts for less than one-sixth of the sequence, and gene loci occupy about one-third of the genome. As with the human genome, gene loci are not evenly distributed, but are clustered into sparse and dense regions. Some "giant" genes were observed that had average coding sequence sizes but were spread over genomic lengths significantly larger than those of their human orthologs. Although three-quarters of predicted human proteins have a strong match to Fugu, approximately a quarter of the human proteins had highly diverged from or had no pufferfish homologs, highlighting the extent of protein evolution in the 450 million years since teleosts and mammals diverged. Conserved linkages between Fugu and human genes indicate the preservation of chromosomal segments from the common vertebrate ancestor, but with considerable scrambling of gene order.     

A human Y-linked DNA polymorphism and its potential for estimating genetic and evolutionary distance

A human DNA sequence (p12f2), derived from a partial Y-chromosome genomic library and showing homology with the X and Y chromosomes and with an undetermined number of autosomes, detected two Y-specific restriction fragment length variants on male DNA that had been digested with Taq I and Eco RI. These variants may have been generated through a deletion-insertion mechanism and their pattern of holoandric transmission indicates that they represent a two-allele Y-linked polymorphism (RFLP). By means of DNA from patients with inborn deletions in chromosome Y, this polymorphic DNA site was mapped to the interval Yq11.1-Yq11.22. The frequency of the rarest allele was about 35 percent in Algerian and Sardinian human males, whereas it was only 4 percent among Northern Europeans. The p12f2 probe also detected Y-specific DNA fragments in the gorilla and chimpanzee. In view of the monosomy of the Y chromosome in mammalian species, Y-linked RFLP's may prove to be more useful than autosomal or X-linked markers in estimating genetic distances within and between species.