全文获取类型
收费全文 | 28917篇 |
免费 | 1532篇 |
国内免费 | 11篇 |
专业分类
林业 | 1199篇 |
农学 | 918篇 |
基础科学 | 195篇 |
4650篇 | |
综合类 | 3681篇 |
农作物 | 1101篇 |
水产渔业 | 1868篇 |
畜牧兽医 | 14596篇 |
园艺 | 376篇 |
植物保护 | 1876篇 |
出版年
2021年 | 286篇 |
2020年 | 277篇 |
2019年 | 384篇 |
2018年 | 624篇 |
2017年 | 663篇 |
2016年 | 646篇 |
2015年 | 463篇 |
2014年 | 618篇 |
2013年 | 1238篇 |
2012年 | 1045篇 |
2011年 | 1228篇 |
2010年 | 845篇 |
2009年 | 854篇 |
2008年 | 1209篇 |
2007年 | 1137篇 |
2006年 | 994篇 |
2005年 | 905篇 |
2004年 | 822篇 |
2003年 | 840篇 |
2002年 | 731篇 |
2001年 | 884篇 |
2000年 | 891篇 |
1999年 | 686篇 |
1998年 | 301篇 |
1997年 | 305篇 |
1995年 | 297篇 |
1992年 | 444篇 |
1991年 | 537篇 |
1990年 | 457篇 |
1989年 | 430篇 |
1988年 | 411篇 |
1987年 | 421篇 |
1986年 | 371篇 |
1985年 | 396篇 |
1984年 | 348篇 |
1983年 | 300篇 |
1982年 | 249篇 |
1979年 | 389篇 |
1978年 | 295篇 |
1977年 | 296篇 |
1976年 | 304篇 |
1975年 | 318篇 |
1974年 | 336篇 |
1973年 | 304篇 |
1972年 | 295篇 |
1971年 | 250篇 |
1969年 | 301篇 |
1968年 | 272篇 |
1967年 | 332篇 |
1966年 | 306篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
171.
A Cirujeda G Pardo A I Marí J Aibar Y Pallavicini J L Gonzlez‐Andújar J Recasens X O Sol‐Senan 《Weed Research》2019,59(4):300-311
172.
J R López S Núñez B Magariños N Castro J I Navas R de la Herran A E Toranzo 《Journal of fish diseases》2009,32(7):603-610
The first isolation of Tenacibaculum maritimum from wedge sole, Dicologoglossa cuneata, is reported. The pathogen was recovered from ulcers of cultured fish, from three different outbreaks. The six isolates obtained were biochemically and serologically characterized and diagnosis was confirmed by polymerase chain reaction using specific primers and partial 16S rRNA gene sequencing. The isolates constituted a homogeneous phenotypic group; however, they belong to two of the different serotypes described within this species. A virulence evaluation of the isolates using Wedge sole fry was also performed. 相似文献
173.
In the Mediterranean area, wild fish have often been suggested as either the reservoirs of the causative agents or at least the carriers of the pathogens responsible for disease outbreaks in cultured fish. However, no epidemiological investigations on actual pathogen/disease interactions between farmed and wild fish have been conducted even for the most important fish pathogens. Only sporadic isolations and identifications of various pathogens in wild fish have been done and real associations with the pathological conditions that exist within the farm environment and vice versa have not been established. Monogenean ectoparasite Sparicotyle chrysophrii and isopod Ceratothoa oestroides are commonly found in the Mediterranean cage-reared sea bream and sea bass and in the surrounding wild fish population. Both species were recognized as pathogens that seasonally inflict serious losses in fingerlings and juveniles of sea bream and sea bass, being potentially propagated and exchanged during wild and caged fish interaction. In order to evaluate the degree of pathogens transfer between wild and farmed fish, we investigated genetic population structures of these two important parasitic pathogens inferred by mtDNA cytochrome oxidase I locus. Parasites isolated from wild and farmed fish on the two most productive Adriatic fish farms showed genetic heterogeneity, contradicting widely accepted hypothesis of cross-contamination, at least in case of S. chrysophrii and C. oestroides. As far as we know, this is the first report that in a study of pathogen transfer molecular evidence was employed to asses the genetic population structure of shared parasites. 相似文献
174.
Lancefield group C Streptococcus dysgalactiae (GCSD) causes severe necrotic lesions in the caudal peduncle in the genus Seriola farmed in Japan. To develop a sero‐diagnostic method for GCSD infection in farmed fish, we attempted to identify a surface immunogenic protein that induces an antibody after infection with GCSD by immunoblot analysis using sera collected from infected fish. A protein obtained from sodium dodecyl sulfate (SDS) extracts of GCSD was identified as S. dysgalactiae surface immunogenic protein (Sd‐Sip). Sd‐Sip exhibited more than 94% homology with a surface antigen or a hypothetical protein from S. dysgalactiae mammalian isolates at the nucleotide sequence level. Expression of the recombinant Sd‐Sip (rSd‐Sip) was confirmed by immunoblot analysis, that is, its reactivity to GCSD‐infected sera. Antibody detection ELISA using rSd‐Sip and their usefulness for diagnosis of GCSD infection were examined. GCSD‐infected sera collected from farmed amberjack, Seriola dumerili (Risso), showed strong reaction with immobilized rSd‐Sip. Meanwhile, sera immunized by other pathogenic bacteria of fish were showed ELISA values similar to those of non‐infected sera. These results of this study suggest that the antibody detection ELISA using rSd‐Sip is an effective diagnostic method for GCSD infection in fish. 相似文献
175.
Rodkhum C Hirono I Stork M Di Lorenzo M Crosa JH Aoki T 《Journal of fish diseases》2006,29(3):157-166
The genome of Vibrio anguillarum strain H775-3 was partially determined by a random sequencing procedure. A total of 2,300 clones, 2,100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence-related genes were identified, 36 of which are novel in V. anguillarum, including a repeat in toxin gene cluster, haemolysin genes, enterobactin gene, protease genes, lipopolysaccharide biosynthesis genes, capsule biosynthesis gene, flagellar genes and pilus genes. 相似文献
176.
Routine metabolic rates of green sturgeon (Acipenser medirostris) from hatching to 31 days post hatch (dph) were determined under normoxic conditions. During the endogenous feeding stage
that comprised period from hatching to 15 dph, the oxygen consumption rate (MO2, μg O2 larva−1 h−1) increased 5-fold before yolk reserves became exhausted and MO2 rates steady. The allometric relationships between MO2 and body mass had mass exponents greater than 1.0 (b=1.64±0.21) and equal to 1.0 (b=1.04±0.07) during the endogenous and exogenous feeding phases, respectively. The magnitude and changes of MO2 rates in green sturgeon larvae reflected their early ontogeny, especially during the endogenous feeding phase when the increase
in metabolic rates was associated with organogenesis, acquisition of organ functions, and the conversion of yolk sac into
new tissues.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
177.
W. D. SUMPTON I. W. BROWN D. G. MAYER M. F. McLENNAN A. MAPLESTON A. R. BUTCHER D. J. WELCH & J. M. KIRKWOOD B. SAWYNOK & G. A. BEGG 《Fisheries Management and Ecology》2010,17(1):77-88
Abstract Common coral trout, Plectropomus leopardus Lacepède, crimson snapper, Lutjanus erythropterus Bloch, saddletail snapper, Lutjanus malabaricus (Bloch & Schneider), red emperor, Lutjanus sebae (Cuvier), redthroat emperor, Lethrinus miniatus (Schneider) and grass emperor, Lethrinus laticaudis Alleyne & Macleay, were tagged to determine the effects of barotrauma relief procedures (weighted shot-line release and venting using a hollow needle) and other factors on survival. Release condition was the most significant factor affecting the subsequent recapture rate of all species. Capture depth was significant in all species apart from L. malabaricus and L. miniatus , the general trend being reduced recapture probability with increasing capture depth. Recapture rates of fish hooked in either the lip or mouth were generally significantly higher than for those hooked in the throat or gut. Statistically significant benefit from treating fish for barotrauma was found in only L. malabaricus , but the lack of any negative effects of treating fish indicated that the practices of venting and shot-lining should not be discouraged by fisheries managers for these species. 相似文献
178.
The growth data of a commercial aquaculture recirculation system were analysed to investigate the growth performance of reared
turbot (Psetta maxima). Three common growth models (von Bertalanffy, Gompertz and Schnute) were fitted to the growth data documented over a time
period of 6 years. To determine the most suitable model, three different criteria were used: (1) the Akaike index criterion,
(2) the sum of squared residuals and (3) the average daily deviation between the estimated final weight and the observed final
weight. The evaluation of the growth models showed that the Schnute model had the lowest Akaike index, the lowest sum of squared
residuals and the lowest daily deviation between estimated and real weight of all tested growth models. The Schnute model
produced sigmoid growth curves. The estimated growth coefficients were the most realistic ones in regard to biological interpretation.
In contrast, the von Bertalanffy growth model and the Gompertz model estimated inaccurate exponential growth curves and are
therefore unable to simulate the growth data as well as the Schnute model. The results indicate that the von Bertalanffy growth
model is not the optimal model to simulate the present growth data and that the growth potential of reared turbot has probably
not yet been fully exploited in the aquaculture system(s) examined (so far). 相似文献
179.
Rocío González Jesús Domingo Celada José Manuel Carral Vanesa García María Sáez‐Royuela Álvaro González 《Aquaculture Research》2011,42(9):1385-1392
Recent advances in crayfish feeding have enabled the development of size grading studies from the start of first‐feeding. A 180‐day experiment aimed at intensive rearing of Pacifastacus leniusculus was carried out under controlled conditions, evaluating the effects of size grading at two different periods from the onset of exogenous feeding. Stage 2 juveniles were stocked in fibreglass tanks at a density of 100 m2, and fed a dry diet for salmonids combined with restricted amounts of Artemia cysts. Five groups were tested: no grading, grading at 60 days (large and small size) and grading at 100 days (large and small size). After 6 months, no significant differences were found in the survival among groups (mean: 73.06%). The highest final growth (pooled results from upper and lower classes: 17.39 mm carapace length, 1.43 g weight) was achieved by the crayfish sorted at 60 days, showing significant differences from the ungraded group. Smaller crayfish graded at 60 days grew significantly faster than smaller crayfish graded at 100 days. The food conversion ratio was lower in the graded groups (mean: 2.64), showing significant differences from the ungraded group (3.23). This study shows that size grading allows a better performance and an improved feeding efficiency. 相似文献
180.